基于HPLC-DAD法及响应曲面法的稻谷叶黄素含量测定方法研究
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摘要
本研究采用HPLC-DAD法,在单因素试验的基础上,用响应曲面法(RSM)对影响稻谷中叶黄素提取效果的四氢呋喃用量、KOH甲醇溶液质量浓度和提取温度三个因素进行优化,建立各因素与叶黄素提取量关系的数学模型。响应曲面法优化稻谷中叶黄素最佳提取方法为四氢呋喃15.5mL、KOH甲醇溶液质量浓度0.1g/mL、提取温度51℃,稻谷中叶黄素提取量为(1.63±0.03)μg/g。运用HPLC-DAD及响应曲面法可有效缩短稻谷中叶黄素提取时间,为高效提取稻谷中的叶黄素提供了参考。
On the basis of single factor experiment, response surface methodology(RSM) was used to optimize the three factors affecting the extraction of lutein from rice, namely the amount of tetrahydrofuran, the concentration of KOH methanol solution and the extraction temperature. The mathematical model of the relationship between each factor and the extraction amount of lutein was established. Response surface methodology was used to optimize the extraction of lutein from paddy. The optimum extraction methods were as follows: the amount of tetrahydrofuran was 15.5 mL, the concentration of KOH methanol solution was 0.10 g/mL, the extraction temperature was 51 ℃, and the extraction amount of lutein from paddy was(1.63±0.03)ug/g. The extraction time of lutein in rice can be effectively shortened by using HPLC-DAD and response surface methodology, which provides a reference for efficient extraction of lutein in rice.
On the basis of single factor experiment, response surface methodology(RSM) was used to optimize the three factors affecting the extraction of lutein from rice, namely the amount of tetrahydrofuran, the concentration of KOH methanol solution and the extraction temperature. The mathematical model of the relationship between each factor and the extraction amount of lutein was established. Response surface methodology was used to optimize the extraction of lutein from paddy. The optimum extraction methods were as follows: the amount of tetrahydrofuran was 15.5 mL, the concentration of KOH methanol solution was 0.10 g/mL, the extraction temperature was 51 ℃, and the extraction amount of lutein from paddy was(1.63±0.03)ug/g. The extraction time of lutein in rice can be effectively shortened by using HPLC-DAD and response surface methodology, which provides a reference for efficient extraction of lutein in rice.
引文
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[15]BOONNOUN P,TUNYASITIKUN P,CLOWUTIMON W,et al.Production of free lutein by simultaneous extraction and de-esterification of marigold flowers in liquefied dimethyl ether(DME)-KOH-EtOH mixture[J].Food&Bioproducts Processing,2017(106):11.
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[19]KIM D E,SHANG X,ASSEFA A D,et al.Metabolite profiling of green,green/red,and red lettuce cultivars:variation in health benefi cial compounds and antioxidant potential[J].Food Research International,2018(5):361-370.
[20]刘春菊,谢晓叶,刘春泉,等.馒头加工和贮藏过程中营养强化剂叶黄素酯的稳定性[J].食品工业科技,2016(11):265-268.
[21]PRABHU A,ABDUL K S,REKHA P D.Isolation and purifi cation of lutein from indian spinach basella alba[J].Research Journal of Pharmacy&Technology,2015(10):1379.
[22]HIDALGO A,BRANDOLINI A,POMPEI C.Carotenoids evolution during pasta,bread and water biscuit preparation from wheat fl ours[J].Food Chemistry,2010(3):746-751.
[23]AHMAD F T,MATHER D E,LAW H Y,et al.Genetic control of lutein esterifi cation in wheat(triticum aestivum,L.)grain[J].Journal of Cereal Science,2015(4):109-115.
[24]ZEB A.A simple,sensitive HPLC-DAD method for simultaneous determination of carotenoids,chlorophylls andα-tocopherol in leafy vegetables[J].Journal of Food Measurement&Characterization,2017(1):1-8.
[25]YUHAS R,MCCORMICK M,YACHETTI S,et al.A method for the measurement of lutein in infant formula[J].Food&Nutrition Sciences,2011(2):145-149.
[26]ALTEMIMI A,LIGHTFOOT D A,KINSEL M,et al.Employing response surface methodology for the optimization of ultrasound assisted extraction of lutein andβ-carotene from spinach[J].Molecules,2015(4):6611-25.
[27]KANG J H,KIM S,MOON B.Optimization by response surface methodology of lutein recovery from paprika leaves using accelerated solvent extraction[J].Food Chemistry,2016(5):140.
[28]MELLADO-ORTEGA E,ATIENZA S G,HORNERO-MéN D E Z D.Carotenoid evolution during postharvest storage of durum wheat(triticum turgidum,conv.durum)and tritordeum(tritordeum,ascherson et graebner)grains[J].Journal of Cereal Science,2015(10):134-142.
[29]庞慧丽,李大婧,刘春泉.叶黄素在有机溶剂中的稳定性[J].食品科学,2013(23):15-19.
[30]杨秋明,宋江峰,李大婧,等.响应面法优化超声波提取南瓜皮叶黄素的工艺研究[J].食品工业科技,2018(1):149-155.
[2]徐秀红,卢华兵,吕桂华,等.玉米黄质的研究进展及展望[J].中国农学通报,2011(2):333-339.
[3]李育楠.玉米叶黄素提取纯化技术研究[D].大庆:黑龙江八一农垦大学,2014.
[4]YUHAS R,MCCORMICK M,YACHETTI S,et al.A method for the measurement of lutein in infant formula[J].Food&Nutrition Sciences,2011(2):145-149.
[5]FERNANDEZ-OROZCO R,GALLARDO-GUERREROL,HORNERO-M??NDEZ D.Carotenoid profiling in tubers of different potato(solanum sp)cultivars:accumulation of carotenoids mediated by xanthophyll esterification[J].Food Chemistry,2013(3):2864-2872.
[6]LOYPIMAI P,MOONGNGARM A,CHOTTANOM P.Phytochemicals and antioxidant capacity of natural food colorant prepared from black waxy rice bran[J].Food Bioscience,2016(15):34-41.
[7]马娜,孙伟鹏,包玲,等.响应面优化盐析萃取万寿菊花中叶黄素的研究[J].中国食品添加剂,2017(6):57-64.
[8]刘会芳,赵元华,王淑静,等.叶黄素抗肿瘤作用的研究进展[J].医学综述,2015(9):1654-1656.
[9]ROBERTS R L.Lutein,zeaxanthin and skin health[J].American Journal of Lifestyle Medicine,2013(3):182-185.
[10]AHMAD F T,ASENSTORFER R E,SORIANO I R,et al.Effect of temperature on lutein esterifi cation and lutein stability inwheat grain[J].Journal of Cereal Science,2013(3):408-413.
[11]PEREIRACARO G,WATANABE S,CROZIER A,et al.Phytochemical profile of a Japanese black-purple rice[J].Food Chemistry,2013(3):2821-2827.
[12]PEREIRACARO G,CROS G,YOKOTA T,et al.Phytochemical profiles of black,red,brown,and white rice from the camargue region of France[J].Journal of Agricultural&Food Chemistry,2013(33):7976-7986.
[13]马空军,潘言亮,张卫红,等.响应面法优化叶黄素酯皂化方法及其动力学特性[J].食品科技,2015(10):197-201.
[14]MA?LLE DERRIEN,MILAD AGHABARARNEJAD,ANDRéGOSSELIN,et al.Optimization of supercritical carbon dioxide extraction of lutein and chlorophyll from spinach by-products using response surface methodology[J].Lwt,2018(3):9-11.
[15]BOONNOUN P,TUNYASITIKUN P,CLOWUTIMON W,et al.Production of free lutein by simultaneous extraction and de-esterification of marigold flowers in liquefied dimethyl ether(DME)-KOH-EtOH mixture[J].Food&Bioproducts Processing,2017(106):11.
[16]FAN X D,HOU Y,HUANG X X,et al.Ultrasound-enhanced subcritical CO2 extraction of lutein from chlorella pyrenoidosa[J].Journal of Agricultural&Food Chemistry,2015(18):4597.
[17]COBB B F,KALLENBACH J,HALL C A,et al.Optimizing the supercritical fluid extraction of lutein from corn gluten meal[J].Food&Bioprocess Technology,2018(4):757-764.
[18]PAL S,BHATTACHARJEE P.Supercritical carbon dioxide extraction of lutein from yellow maize(Zea mays)kernels:process optimization based on lutein content,antioxidant activity,andω-6/ω-3fatty acid ratio[J].Food Science&Biotechnology,2017(6):1-11.
[19]KIM D E,SHANG X,ASSEFA A D,et al.Metabolite profiling of green,green/red,and red lettuce cultivars:variation in health benefi cial compounds and antioxidant potential[J].Food Research International,2018(5):361-370.
[20]刘春菊,谢晓叶,刘春泉,等.馒头加工和贮藏过程中营养强化剂叶黄素酯的稳定性[J].食品工业科技,2016(11):265-268.
[21]PRABHU A,ABDUL K S,REKHA P D.Isolation and purifi cation of lutein from indian spinach basella alba[J].Research Journal of Pharmacy&Technology,2015(10):1379.
[22]HIDALGO A,BRANDOLINI A,POMPEI C.Carotenoids evolution during pasta,bread and water biscuit preparation from wheat fl ours[J].Food Chemistry,2010(3):746-751.
[23]AHMAD F T,MATHER D E,LAW H Y,et al.Genetic control of lutein esterifi cation in wheat(triticum aestivum,L.)grain[J].Journal of Cereal Science,2015(4):109-115.
[24]ZEB A.A simple,sensitive HPLC-DAD method for simultaneous determination of carotenoids,chlorophylls andα-tocopherol in leafy vegetables[J].Journal of Food Measurement&Characterization,2017(1):1-8.
[25]YUHAS R,MCCORMICK M,YACHETTI S,et al.A method for the measurement of lutein in infant formula[J].Food&Nutrition Sciences,2011(2):145-149.
[26]ALTEMIMI A,LIGHTFOOT D A,KINSEL M,et al.Employing response surface methodology for the optimization of ultrasound assisted extraction of lutein andβ-carotene from spinach[J].Molecules,2015(4):6611-25.
[27]KANG J H,KIM S,MOON B.Optimization by response surface methodology of lutein recovery from paprika leaves using accelerated solvent extraction[J].Food Chemistry,2016(5):140.
[28]MELLADO-ORTEGA E,ATIENZA S G,HORNERO-MéN D E Z D.Carotenoid evolution during postharvest storage of durum wheat(triticum turgidum,conv.durum)and tritordeum(tritordeum,ascherson et graebner)grains[J].Journal of Cereal Science,2015(10):134-142.
[29]庞慧丽,李大婧,刘春泉.叶黄素在有机溶剂中的稳定性[J].食品科学,2013(23):15-19.
[30]杨秋明,宋江峰,李大婧,等.响应面法优化超声波提取南瓜皮叶黄素的工艺研究[J].食品工业科技,2018(1):149-155.