毛细管区带电泳法分析药桑多糖中单糖的组成
|
摘要
目的了解高效毛细管区带电泳法分析药桑多糖中单糖组成的作用。方法将药桑多糖经酸水解后,经1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生化,采用高效毛细管电泳法分析各单糖组成。电泳条件:180 mmol/L硼酸缓冲液溶液,pH=10.05,柱温25℃,电压15kV,气压进样3.447 5kPa下进样10s,检测波长245nm。结果药桑多糖中主要由木糖、阿拉伯糖、葡萄糖、鼠李糖、甘露糖、半乳糖、半乳糖醛酸组成,其物质的量之比为1∶7.4∶15.8∶3.4∶10.8∶14.3∶4.5。结论高效毛细管电泳法用于多糖中单糖的组成分析具有快速、简便、精度高的特点,可用于药桑多糖中单糖组成测定和质量控制。
Objective The High Performance Capillary Zone Electrophoresis was used to analysis monosaccharide composition in polysaccharide of Morus nigra Linn.Methods The polysaccharide of Morus nigra Linn were derivatization with 1-Phenyl-3-methyl-5-pyrazolone(PMP) after hydrolyzed into monosaccharides with Trifluoroacetic Acid(TFA)and were separated by HPCE.The CE condition:Buffer:180mmol/L boric acid solution(pH 10.05),column temperature:25℃;Voltage:15kV;injection time:3.447 5kPa×10s,the detection:245nm.Results The polysaccharide was mainly composed of xylose,Arabinose,Glucose,Rhamnose,Mannose,Galactose,GalUA.The amount of the substance is 1∶7.4∶15.8∶3.4∶10.8∶14.3∶4.5.Conclusion High performance capillary electrophoresis method for the analysis of polysaccharide is rapid,simple and high accuracy.It can be used for the determination and quality control of polysaccharide in Morus nigra Linn.
Objective The High Performance Capillary Zone Electrophoresis was used to analysis monosaccharide composition in polysaccharide of Morus nigra Linn.Methods The polysaccharide of Morus nigra Linn were derivatization with 1-Phenyl-3-methyl-5-pyrazolone(PMP) after hydrolyzed into monosaccharides with Trifluoroacetic Acid(TFA)and were separated by HPCE.The CE condition:Buffer:180mmol/L boric acid solution(pH 10.05),column temperature:25℃;Voltage:15kV;injection time:3.447 5kPa×10s,the detection:245nm.Results The polysaccharide was mainly composed of xylose,Arabinose,Glucose,Rhamnose,Mannose,Galactose,GalUA.The amount of the substance is 1∶7.4∶15.8∶3.4∶10.8∶14.3∶4.5.Conclusion High performance capillary electrophoresis method for the analysis of polysaccharide is rapid,simple and high accuracy.It can be used for the determination and quality control of polysaccharide in Morus nigra Linn.
引文
[1]买买提依明.新疆药桑的研究[J].北方蚕业,2007,28(1):1-3.
[2]买买提依明,卢红,木合达尔,等.新疆药桑的有效成分测定及药理作用的研究初报[J].北方蚕业,2006,27(4):16-18.
[3]新疆维吾尔自治区维吾尔医药研究所.中华本草·维吾尔药卷[M].上海:上海科学技术出版社,2005:325-326.
[4]吴志平,周巧霞,顾振纶,等.桑树不同药用部位的降血糖效果比较[J].蚕业科学,2005,31(2):215-217.
[5]宋大可,买买提依明·乃比江.新疆药桑果汁化学成份及药物价值探讨[J].蚕桑通报,1994,25(4):23-34.
[6]刘勇民.维吾尔药志[M].乌鲁木齐:新疆科技卫生出版社,1999:43-47.
[7]夏娜,陈义磊,陶海燕,等.药桑多糖对大鼠肝损伤的保护作用[J].食品科学,2015,36(13):247-251.
[8]宋峰峰,倪雪雪,魏鸿,等.基于FTIR的药桑主成分分析[J].新疆医科大学学报[J].2014,37(5):565-567.
[9]王贺,刘健健,杨俊玲,等.新疆药桑不同部位降血糖活性研究[J].广州化工,2014,42(14):76-78.
[10]于小蓉,孙莲,孟磊.新疆药桑叶单糖组成的气相色谱分析[J].西北药学杂志,2013,28(1):25-29.
[2]买买提依明,卢红,木合达尔,等.新疆药桑的有效成分测定及药理作用的研究初报[J].北方蚕业,2006,27(4):16-18.
[3]新疆维吾尔自治区维吾尔医药研究所.中华本草·维吾尔药卷[M].上海:上海科学技术出版社,2005:325-326.
[4]吴志平,周巧霞,顾振纶,等.桑树不同药用部位的降血糖效果比较[J].蚕业科学,2005,31(2):215-217.
[5]宋大可,买买提依明·乃比江.新疆药桑果汁化学成份及药物价值探讨[J].蚕桑通报,1994,25(4):23-34.
[6]刘勇民.维吾尔药志[M].乌鲁木齐:新疆科技卫生出版社,1999:43-47.
[7]夏娜,陈义磊,陶海燕,等.药桑多糖对大鼠肝损伤的保护作用[J].食品科学,2015,36(13):247-251.
[8]宋峰峰,倪雪雪,魏鸿,等.基于FTIR的药桑主成分分析[J].新疆医科大学学报[J].2014,37(5):565-567.
[9]王贺,刘健健,杨俊玲,等.新疆药桑不同部位降血糖活性研究[J].广州化工,2014,42(14):76-78.
[10]于小蓉,孙莲,孟磊.新疆药桑叶单糖组成的气相色谱分析[J].西北药学杂志,2013,28(1):25-29.