双波长薄层色谱扫描法测定刺糖中蔗果三糖的含量
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摘要
目的建立双波长薄层色谱扫描法测定不同时间、产地刺糖中蔗果三糖(GF2)含量。方法以GF2为标准品,采用双波长薄层扫描分析,展开剂为冰乙酸-三氯甲烷-95%乙醇(9∶11∶11),显色剂为二苯胺-苯胺-丙酮-磷酸(1∶1∶50∶5),最大吸收波长510nm,参比波长610nm。结果蔗果三糖(GF2)在0.642 8~15.975 2μg范围内线性关系良好,r=0.999 2,平均回收率为102.5%,RSD为1.55%。不同产地刺糖中GF2含量为在12.957 3~36.328 1mg/g。结论双波长薄层色谱扫描法分离效果好,操作简便,可用于刺糖中GF2的定性及定量分析。
Objective To establish the method for the determination of kestose in different time and origin of Alhagi-honey by Dual Wave length TLC-Scanning.Methods Dual Wavelength TLCS canner was selected with GF2 as standard substance.The developing solvent was glacial acetic acid-trichloromethane-95% ethanol(9∶11∶11).The color developing agent was diphenylamine-aniline-acetone-phosphoric acid(1∶1∶50∶5);λS=510nm,λR=610nm.Results The calibration curve was liner in range of 0.642 8-15.975 2μg for kestose(r =0.999 2).The average recovery was 102.5%,and RSD was 1.55%.The content of GF2 from different places of origin habitats in Alhagi-honeywere between 12.957 3-36.328 1mg/g.Conclusion The established method has a good separation effect and simple operation,so can be used for the qualitative and quantitative analysis of kestose from Alhagi-honey.
Objective To establish the method for the determination of kestose in different time and origin of Alhagi-honey by Dual Wave length TLC-Scanning.Methods Dual Wavelength TLCS canner was selected with GF2 as standard substance.The developing solvent was glacial acetic acid-trichloromethane-95% ethanol(9∶11∶11).The color developing agent was diphenylamine-aniline-acetone-phosphoric acid(1∶1∶50∶5);λS=510nm,λR=610nm.Results The calibration curve was liner in range of 0.642 8-15.975 2μg for kestose(r =0.999 2).The average recovery was 102.5%,and RSD was 1.55%.The content of GF2 from different places of origin habitats in Alhagi-honeywere between 12.957 3-36.328 1mg/g.Conclusion The established method has a good separation effect and simple operation,so can be used for the qualitative and quantitative analysis of kestose from Alhagi-honey.
引文
[1]菅丽君,常军民,李改茹.柱前衍生毛细管电泳法测定刺糖多糖的单糖组成[J].中国新药杂志,2012,21(5):78-81.
[2]程煜凤.维药刺糖化学成分的基础研究[D].乌鲁木齐:新疆医科大学,2010.
[3]Mabel MJ,Sangeetha PT,Kalpana P,et al.Physicochemical characterization of fructooligosaccharides and evaluation of their suitability as a potential sweetener for diabetics[J].Carbohydrate Res,2008,3(1):56-59.
[4]Jackson CL,Dreaden TM,Theobald LK,et al.Pectininduces apoptosis inhuman prostate cancer cells:correlation of apoptotic function with pectin structure[J].Glycobiology,2007,17(8):805-819.
[5]陈亚非,罗琦珊,葛亚中.低聚果糖调节机体免疫功能作用的研究进展[J].现代食品科技,2005,21(4):83-87.
[6]吴晶,李改茹,常军民,等.刺糖中多糖的提取工艺研究[J].中成药,2011,33(5):902-904.
[7]郑杰,李改茹,程煜凤,等.HPLC/ESI-MS法测定刺糖中低聚果糖的含量[J].中药新药与临床药理,2015,28(5):671-675.
[8]李继民,王彦吉,邹宁,等.薄层色谱扫描法测定复印纸中单糖的研究[J].分析试验室,2008,27(11):451-455.
[9]达丽.维药刺糖中糖类化合物的分离与鉴定[J].健康必读,2012,11(8):95-96.
[2]程煜凤.维药刺糖化学成分的基础研究[D].乌鲁木齐:新疆医科大学,2010.
[3]Mabel MJ,Sangeetha PT,Kalpana P,et al.Physicochemical characterization of fructooligosaccharides and evaluation of their suitability as a potential sweetener for diabetics[J].Carbohydrate Res,2008,3(1):56-59.
[4]Jackson CL,Dreaden TM,Theobald LK,et al.Pectininduces apoptosis inhuman prostate cancer cells:correlation of apoptotic function with pectin structure[J].Glycobiology,2007,17(8):805-819.
[5]陈亚非,罗琦珊,葛亚中.低聚果糖调节机体免疫功能作用的研究进展[J].现代食品科技,2005,21(4):83-87.
[6]吴晶,李改茹,常军民,等.刺糖中多糖的提取工艺研究[J].中成药,2011,33(5):902-904.
[7]郑杰,李改茹,程煜凤,等.HPLC/ESI-MS法测定刺糖中低聚果糖的含量[J].中药新药与临床药理,2015,28(5):671-675.
[8]李继民,王彦吉,邹宁,等.薄层色谱扫描法测定复印纸中单糖的研究[J].分析试验室,2008,27(11):451-455.
[9]达丽.维药刺糖中糖类化合物的分离与鉴定[J].健康必读,2012,11(8):95-96.