毛细管电泳技术的发展与应用
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摘要
毛细管电泳法具有多分离模式、高分离效率、快速检测、易于调控以及环境污染小等优点,是发展最为迅速的分离分析技术手段之一。当前毛细管电泳法处于稳步发展的状态,相关标准方法也陆续出台,其主要用于食品中大分子以及成分较为复杂的中医药分析等。与传统的色谱方法相比,毛细管电泳法对于成分较复杂的样品以及生物大分子的分析检测更为便利,实用性较强,同时其检测能力范围不低于液相色谱,具有较为广阔的应用前景。本文主要介绍了毛细管电泳的相关标准和在食品安全、中医药方面的应用。
As one of the fastest growing separation techniques, capillary electrophoresis(CE) has the advantages of multiple separation modes, high separation efficiency, rapid analysis, easy control and low environmental pollution, etc. Nowadays, CE is at a state of steady development, and the relevant standard methods are gradually introduced. CE is mainly used in the analysis of food macromolecules and the complex compositions of traditional Chinese medicine. Compared with traditional chromatographic methods, CE is more convenient and practical for the analysis and detection of complex components of the samples and biological macromolecules, while the detection range is not less than liquid chromatographic method, and it has wide application prospects. This paper mainly introduced the related standards of capillary electrophoresis and its application in food safety and traditional Chinese medicine.
As one of the fastest growing separation techniques, capillary electrophoresis(CE) has the advantages of multiple separation modes, high separation efficiency, rapid analysis, easy control and low environmental pollution, etc. Nowadays, CE is at a state of steady development, and the relevant standard methods are gradually introduced. CE is mainly used in the analysis of food macromolecules and the complex compositions of traditional Chinese medicine. Compared with traditional chromatographic methods, CE is more convenient and practical for the analysis and detection of complex components of the samples and biological macromolecules, while the detection range is not less than liquid chromatographic method, and it has wide application prospects. This paper mainly introduced the related standards of capillary electrophoresis and its application in food safety and traditional Chinese medicine.
引文
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[12]SH/T 1687-2000工业用精对苯二甲酸(PTA)中对羧基苯甲醛和对甲基苯甲酸含量的测定高效毛细管电泳法(HPCE)[S].SH/T 1687-2000 Purified terephthalic acid(PTA)for industrial use—Determination of 4-a Crboxybenzaldehyde and p-toluic acid-High performance capillary electrophoresis(HPCE)[S].
[13]NY/T 3001-2016饲料中氨基酸的测定毛细管电泳法[S].NY/T 3001-2016 Determination of amino acids in feeds-Capillary electrophoresis[S].
[14]董亚蕾,陈晓娇,胡敬,等.高效毛细管电泳在食品安全检测中的应用进展[J].色谱,2012,30(11):1117?1126.Dong YL,Chen XJ,Hu J,et al.Recent advances in the application of high performance capillary electrophoresis for food safety[J].Chin J Chromatogr,2012,30(11):1117?1126.
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