海洋生物小GTPase蛋白家族研究进展
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摘要
小GTPase蛋白家族是一类普遍存在于真核生物的GTP结合蛋白超家族,具有众多家族成员,按照其序列结构特征和生物学功能的不同,主要可以分为Ras、Rho、Rab、Ran和Arf 5个亚家族。诸多研究显示,小GTPase蛋白家族成员在细胞分化、生长发育、肿瘤发生等过程中均发挥着重要作用。小GTPase蛋白家族成员在调控海洋生物细胞分化、骨骼发育和免疫应答等生理生化过程中的作用受到人们的广泛关注。本研究中综述了近年来对海洋生物中小GTPase蛋白家族成员的研究进展,包括其种类、功能和作用机制,以期为进一步深入了解海洋生物小GTPase蛋白家族成员的基因序列特征、进化特点和生物学功能提供更多的信息和资料。
Small GTPases(containing more than 100 members) as a large superfamily in eukaryotic cells can be classified into five subfamilies(Ras, Rho, Rab, Ran and Arf) according to their sequence characterization and bio-functions and play vital roles in various physiological processes of organisms, including cell differentiation, growth and development, and tumorigenesis, especially in cell differentiation, cytoskeleton development and innate immune responses in marine animals. In this study, we reviewed the recent advances on the research of small GTPase family members in marine organisms, including species composition, functions and mechanisms to provide more information and data for further understanding of the gene sequence, evolutionary characteristics and biological functions of members in the small GTPase family in marine organisms.
Small GTPases(containing more than 100 members) as a large superfamily in eukaryotic cells can be classified into five subfamilies(Ras, Rho, Rab, Ran and Arf) according to their sequence characterization and bio-functions and play vital roles in various physiological processes of organisms, including cell differentiation, growth and development, and tumorigenesis, especially in cell differentiation, cytoskeleton development and innate immune responses in marine animals. In this study, we reviewed the recent advances on the research of small GTPase family members in marine organisms, including species composition, functions and mechanisms to provide more information and data for further understanding of the gene sequence, evolutionary characteristics and biological functions of members in the small GTPase family in marine organisms.
引文
[1] 张冬梅.稻瘟病菌Rab蛋白功能研究[D].福州:福建农林大学,2009.
[2] Seabra M C,Wasmeier C.Controlling the location and activation of Rab GTPases[J].Current Opinion in Cell Biology,2004,16(4):451-457.
[3] Chang E H,Gonda M A,Ellis R W,et al.Human genome contains four genes homologous to transforming genes of Harvey and Kirsten murine sarcoma viruses[J].Proceedings of the National Academy of Sciences of the United States of America,1982,79(16):4848-4852.
[4] Madaule P,Axel R.A novel ras-related gene family[J].Cell,1985,41(1):31-40.
[5] Munemitsu S,Innis M A,Clark R,et al.Molecular cloning and expression of a G25K cDNA,the human homolog of the yeast cell cycle gene CDC42[J].Molecular and Cellular Biology,1990,10(11):5977-5982.
[6] Wennerberg K,Rossman K L,Der C J.The Ras superfamily at a glance[J].Journal of Cell Science,2005,118(Pt 5):843-846.
[7] Bustelo X R,Sauzeau V,Berenjeno I M.GTP-binding proteins of the Rho/Rac family:regulation,effectors and functions in vivo[J].Bio Essays,2007,29(4):356-370.
[8] Stenmark H,Olkkonen V M.The Rab GTPase family[J].Genome Biology,2001,2(5):3007.1-3007.7
[9] Avis J M,Clarke P R.Ran,a GTPase involved in nuclear processes:its regulators and effectors[J].Journal of Cell Science,1996,109(Pt 10):2423-2427.
[10] Goodsell D S.The molecular perspective:the ras oncogene[J].The Oncologist,1999,4(3):263-264.
[11] Donaldson J G,Honda A.Localization and function of Arf family GTPases[J].Biochemical Society Transactions,2005,33(Pt 4):639-642.
[12] Ridley A J.Rho GTPase signalling in cell migration[J].Current Opinion in Cell Biology,2015,36:103-112.
[13] Ridley A J.Anne ridley:networking with Rho GTPases[J].Trends in Cell Biology,2016,26(7):465-466.
[14] Heasman S J,Ridley A J.Mammalian Rho GTPases:new insights into their functions from in vivo studies[J].Nature Reviews Molecular Cell Biology,2008,9(9):690-701.
[15] 孙中雅.靶向肿瘤相关重要蛋白BRD4和RhoA小分子抑制剂的发现及作用机制的研究[D].南昌:南昌大学,2017.
[16] Boureux A,Vignal E,Faure S,et al.Evolution of the Rho family of Ras-Like GTPases in eukaryotes[J].Molecular Biology and Evolution,2007,24(1):203-216.
[17] Li Kaiquan,Liu Lin,Shang Shengnan,et al.cDNA cloning,expression and immune function analysis of a novel Rac1 gene (AjRac1) in the sea cucumber Apostichopus japonicus[J].Fish & Shellfish Immunology,2017,69:218-226.
[18] Cha Guihong,Wang Weina,Peng Ting,et al.A Rac1 GTPase is a critical factor in the immune response of shrimp (Litopenaeus vannamei) to Vibrio alginolyticus infection[J].Developmental & Comparative Immunology,2015,51(2):226-237.
[19] 刘岚萍.大黄鱼Rac、Rab7和Rab5A基因的分子特征与免疫功能的研究[D].厦门:集美大学,2017.
[20] Nohata N,Uchida Y,Stratman A N,et al.Temporal-specific roles of Rac1 during vascular development and retinal angiogenesis[J]. Developmental Biology,2016,411(2):183-194.
[21] Goicoechea S M,Zinn A,Awadia S S,et al.A RhoG-mediated signaling pathway that modulates invadopodiadynamics in breast cancer cells[J].Journal of Cell Science,2017,130(6):1064.
[22] Allaire K M,Watson G M.Rho participates in chemoreceptor-induced changes in morphology to hair bundle mechanoreceptors of the sea anemone,Nematostella vectensis[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2013,165(2):139-148.
[23] Gray S M,Kelly S,Robles L J.Rho signaling mediates cytoskeletal re-arrangements in octopus photoreceptors[J].American Malacological Bulletin,2008,26(1-2):19-26.
[24] Aguirre-Armenta B,López-Godínez J,Martínez-Cadena G,et al.Rho-kinase in sea urchin eggs and embryos[J].Development,Growth & Differentiation,2011,53(5):704-714.
[25] Li Xue,Wang Ruijia,Xun Xiaogang,et al.The Rho GTPase family genes in bivalvia genomes:sequence,evolution and expression analysis[J].PLoS One,2015,10(12):e0143932.
[26] Muramatsu T,Kozaki K I,Imoto S,et al.The hypusine cascade promotes cancer progression and metastasis through the regulation of RhoA in squamous cell carcinoma[J].Oncogene, 2016,35(40):5304-5316.
[27] Peng Ting,Wang Weina,Gu Meimei,et al.Essential roles of Cdc42 and MAPK in cadmium-induced apoptosis in Litopenaeus vannamei[J]. Aquatic Toxicology,2015,163:89-96.
[28] 李媛,史册,张雪,等.细胞极性相关蛋白CDC42和PAR3在小鼠牙胚发育过程中的表达[J].吉林大学学报(医学版), 2018(1):41-44.
[29] Seabra M C,Mules E H,Hume A N.Rab GTPases,intracellular traffic and disease[J].Trends in Molecular Medicine,2002,8(1):23-30.
[30] Yu E,Kanno E,Choi S,et al.Role of Rab27 in synaptic transmission at the squid giant synapse[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(41):16003-16008.
[31] W?llert T,Patel A,Lee Y L,et al.Myosin5a tail associates directly with Rab3A-containing compartments in neurons[J].Journal of Biological Chemistry,2011,286(16):14352-14361.
[32] Wang Lei,Wang Xiaorong,Liu Jin,et al.Rab from the white shrimp Litopenaeus vannamei:characterization and its regulation upon environmental stress[J].Ecotoxicology,2015,24(7-8):1765-1774.
[33] 向赟,吴宇学,王刚,等.不同温度下哈维氏弧菌和白斑综合症病毒对凡纳滨对虾的致病性[J].大连海洋大学学报,2015,30(1):56-61.
[34] Hino H,Arimoto K,Yazawa M,et al.Ran and calcineurin can participate collaboratively in the regulation of spermatogenesis in scallop[J].Marine Biotechnology,2012,14(4):479-490.
[35] Zhou Falin,Zheng Liming,Yang Qibin,et al.Molecular analysis of a Ras-like nuclear (Ran) gene from Penaeus monodon and its expression at the different ovarian stages of development[J].Molecular Biology Reports,2012,39(4):3821-3827.
[36] Zhao Zhe,Jiang Chunxia,Zhang Xiaobo.Effects of immunostimulants targeting Ran GTPase on phagocytosis against virus infection in shrimp[J].Fish & Shellfish Immunology,2011,31(6):1013-1018.
[37] Liu Weifeng,Han Fang,Zhang Xiaobo.Ran GTPase regulates hemocytic phagocytosis of shrimp by interaction with myosin[J].Journal of Proteome Research,2009,8(3):1198-1206.
[38] Fang Hang,Wang Xiaoqing,Yao Cuiluan,et al.Molecular characterization of Ran gene up-regulated in large yellow croaker (Pseudosciaena crocea) immunity[J].Fish & Shellfish Immunology,2010,29(2):327-333.
[39] Jouaux A,Franco A,Heude-Berthelin C,et al.Identification of Ras,Pten and p70S6K homologs in the Pacific oyster Crassostrea gigas and diet control of insulin pathway[J].General and Comparative Endocrinology,2012,176(1):28-38.
[40] Di Yanan,Schroeder D C,Highfield A,et al.Tissue-specific expression of p53 and Ras genes in response to the environmental genotoxicant Benzo(α)pyrene in marine mussels[J].Environmental Science & Technology,2011,45(20):8974-8981.
[41] Di Yanan,Aminot Y,Schroeder D C,et al.Integrated biological responses and tissue-specific expression of p53 and Ras genes in marine mussels following exposure to benzo(α)pyrene and C60 fullerenes,either alone or in combination[J].Mutagenesis,2017,32(1):77-90.
[42] Downward J.Targeting RAS signalling pathways in cancer therapy[J].Nature Reviews Cancer,2003,3(1):11-22.
[43] Davis J E,Xie Xiayang,Guo Jianhui,et al.ARF1 promotes prostate tumorigenesis via targeting oncogenic MAPK signaling[J].Oncotarget,2016,7(26):39834-39845.
[44] Stepicheva N A,Dumas M,Kobi P,et al.The small GTPase Arf6 regulates sea urchin morphogenesis[J].Differentiation,2017,95:31-43.
[45] Sané A T,Seidman E,Peretti N,et al.Understanding chylomicron retention disease through Sar1b GTPase gene disruption:insight from cell culture[J].Arteriosclerosis,Thrombosis,and Vascular Biology,2017,37(12):2243-2251.
[2] Seabra M C,Wasmeier C.Controlling the location and activation of Rab GTPases[J].Current Opinion in Cell Biology,2004,16(4):451-457.
[3] Chang E H,Gonda M A,Ellis R W,et al.Human genome contains four genes homologous to transforming genes of Harvey and Kirsten murine sarcoma viruses[J].Proceedings of the National Academy of Sciences of the United States of America,1982,79(16):4848-4852.
[4] Madaule P,Axel R.A novel ras-related gene family[J].Cell,1985,41(1):31-40.
[5] Munemitsu S,Innis M A,Clark R,et al.Molecular cloning and expression of a G25K cDNA,the human homolog of the yeast cell cycle gene CDC42[J].Molecular and Cellular Biology,1990,10(11):5977-5982.
[6] Wennerberg K,Rossman K L,Der C J.The Ras superfamily at a glance[J].Journal of Cell Science,2005,118(Pt 5):843-846.
[7] Bustelo X R,Sauzeau V,Berenjeno I M.GTP-binding proteins of the Rho/Rac family:regulation,effectors and functions in vivo[J].Bio Essays,2007,29(4):356-370.
[8] Stenmark H,Olkkonen V M.The Rab GTPase family[J].Genome Biology,2001,2(5):3007.1-3007.7
[9] Avis J M,Clarke P R.Ran,a GTPase involved in nuclear processes:its regulators and effectors[J].Journal of Cell Science,1996,109(Pt 10):2423-2427.
[10] Goodsell D S.The molecular perspective:the ras oncogene[J].The Oncologist,1999,4(3):263-264.
[11] Donaldson J G,Honda A.Localization and function of Arf family GTPases[J].Biochemical Society Transactions,2005,33(Pt 4):639-642.
[12] Ridley A J.Rho GTPase signalling in cell migration[J].Current Opinion in Cell Biology,2015,36:103-112.
[13] Ridley A J.Anne ridley:networking with Rho GTPases[J].Trends in Cell Biology,2016,26(7):465-466.
[14] Heasman S J,Ridley A J.Mammalian Rho GTPases:new insights into their functions from in vivo studies[J].Nature Reviews Molecular Cell Biology,2008,9(9):690-701.
[15] 孙中雅.靶向肿瘤相关重要蛋白BRD4和RhoA小分子抑制剂的发现及作用机制的研究[D].南昌:南昌大学,2017.
[16] Boureux A,Vignal E,Faure S,et al.Evolution of the Rho family of Ras-Like GTPases in eukaryotes[J].Molecular Biology and Evolution,2007,24(1):203-216.
[17] Li Kaiquan,Liu Lin,Shang Shengnan,et al.cDNA cloning,expression and immune function analysis of a novel Rac1 gene (AjRac1) in the sea cucumber Apostichopus japonicus[J].Fish & Shellfish Immunology,2017,69:218-226.
[18] Cha Guihong,Wang Weina,Peng Ting,et al.A Rac1 GTPase is a critical factor in the immune response of shrimp (Litopenaeus vannamei) to Vibrio alginolyticus infection[J].Developmental & Comparative Immunology,2015,51(2):226-237.
[19] 刘岚萍.大黄鱼Rac、Rab7和Rab5A基因的分子特征与免疫功能的研究[D].厦门:集美大学,2017.
[20] Nohata N,Uchida Y,Stratman A N,et al.Temporal-specific roles of Rac1 during vascular development and retinal angiogenesis[J]. Developmental Biology,2016,411(2):183-194.
[21] Goicoechea S M,Zinn A,Awadia S S,et al.A RhoG-mediated signaling pathway that modulates invadopodiadynamics in breast cancer cells[J].Journal of Cell Science,2017,130(6):1064.
[22] Allaire K M,Watson G M.Rho participates in chemoreceptor-induced changes in morphology to hair bundle mechanoreceptors of the sea anemone,Nematostella vectensis[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2013,165(2):139-148.
[23] Gray S M,Kelly S,Robles L J.Rho signaling mediates cytoskeletal re-arrangements in octopus photoreceptors[J].American Malacological Bulletin,2008,26(1-2):19-26.
[24] Aguirre-Armenta B,López-Godínez J,Martínez-Cadena G,et al.Rho-kinase in sea urchin eggs and embryos[J].Development,Growth & Differentiation,2011,53(5):704-714.
[25] Li Xue,Wang Ruijia,Xun Xiaogang,et al.The Rho GTPase family genes in bivalvia genomes:sequence,evolution and expression analysis[J].PLoS One,2015,10(12):e0143932.
[26] Muramatsu T,Kozaki K I,Imoto S,et al.The hypusine cascade promotes cancer progression and metastasis through the regulation of RhoA in squamous cell carcinoma[J].Oncogene, 2016,35(40):5304-5316.
[27] Peng Ting,Wang Weina,Gu Meimei,et al.Essential roles of Cdc42 and MAPK in cadmium-induced apoptosis in Litopenaeus vannamei[J]. Aquatic Toxicology,2015,163:89-96.
[28] 李媛,史册,张雪,等.细胞极性相关蛋白CDC42和PAR3在小鼠牙胚发育过程中的表达[J].吉林大学学报(医学版), 2018(1):41-44.
[29] Seabra M C,Mules E H,Hume A N.Rab GTPases,intracellular traffic and disease[J].Trends in Molecular Medicine,2002,8(1):23-30.
[30] Yu E,Kanno E,Choi S,et al.Role of Rab27 in synaptic transmission at the squid giant synapse[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(41):16003-16008.
[31] W?llert T,Patel A,Lee Y L,et al.Myosin5a tail associates directly with Rab3A-containing compartments in neurons[J].Journal of Biological Chemistry,2011,286(16):14352-14361.
[32] Wang Lei,Wang Xiaorong,Liu Jin,et al.Rab from the white shrimp Litopenaeus vannamei:characterization and its regulation upon environmental stress[J].Ecotoxicology,2015,24(7-8):1765-1774.
[33] 向赟,吴宇学,王刚,等.不同温度下哈维氏弧菌和白斑综合症病毒对凡纳滨对虾的致病性[J].大连海洋大学学报,2015,30(1):56-61.
[34] Hino H,Arimoto K,Yazawa M,et al.Ran and calcineurin can participate collaboratively in the regulation of spermatogenesis in scallop[J].Marine Biotechnology,2012,14(4):479-490.
[35] Zhou Falin,Zheng Liming,Yang Qibin,et al.Molecular analysis of a Ras-like nuclear (Ran) gene from Penaeus monodon and its expression at the different ovarian stages of development[J].Molecular Biology Reports,2012,39(4):3821-3827.
[36] Zhao Zhe,Jiang Chunxia,Zhang Xiaobo.Effects of immunostimulants targeting Ran GTPase on phagocytosis against virus infection in shrimp[J].Fish & Shellfish Immunology,2011,31(6):1013-1018.
[37] Liu Weifeng,Han Fang,Zhang Xiaobo.Ran GTPase regulates hemocytic phagocytosis of shrimp by interaction with myosin[J].Journal of Proteome Research,2009,8(3):1198-1206.
[38] Fang Hang,Wang Xiaoqing,Yao Cuiluan,et al.Molecular characterization of Ran gene up-regulated in large yellow croaker (Pseudosciaena crocea) immunity[J].Fish & Shellfish Immunology,2010,29(2):327-333.
[39] Jouaux A,Franco A,Heude-Berthelin C,et al.Identification of Ras,Pten and p70S6K homologs in the Pacific oyster Crassostrea gigas and diet control of insulin pathway[J].General and Comparative Endocrinology,2012,176(1):28-38.
[40] Di Yanan,Schroeder D C,Highfield A,et al.Tissue-specific expression of p53 and Ras genes in response to the environmental genotoxicant Benzo(α)pyrene in marine mussels[J].Environmental Science & Technology,2011,45(20):8974-8981.
[41] Di Yanan,Aminot Y,Schroeder D C,et al.Integrated biological responses and tissue-specific expression of p53 and Ras genes in marine mussels following exposure to benzo(α)pyrene and C60 fullerenes,either alone or in combination[J].Mutagenesis,2017,32(1):77-90.
[42] Downward J.Targeting RAS signalling pathways in cancer therapy[J].Nature Reviews Cancer,2003,3(1):11-22.
[43] Davis J E,Xie Xiayang,Guo Jianhui,et al.ARF1 promotes prostate tumorigenesis via targeting oncogenic MAPK signaling[J].Oncotarget,2016,7(26):39834-39845.
[44] Stepicheva N A,Dumas M,Kobi P,et al.The small GTPase Arf6 regulates sea urchin morphogenesis[J].Differentiation,2017,95:31-43.
[45] Sané A T,Seidman E,Peretti N,et al.Understanding chylomicron retention disease through Sar1b GTPase gene disruption:insight from cell culture[J].Arteriosclerosis,Thrombosis,and Vascular Biology,2017,37(12):2243-2251.