SRAP分子标记预测萝卜遗传距离与杂种优势的相关性
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摘要
探讨应用SRAP分子标记分析萝卜亲本遗传距离从而预测F1杂种优势的可行性,为萝卜杂交种亲本的性状改良及F1杂种优势选配提供参考。选择来源不同、农艺性状有差异的11个耐抽薹萝卜亲本材料,按不完全双列杂交法组配30个杂交组合,运用SRAP分子标记分析萝卜亲本遗传距离以及其和萝卜农艺性状杂种优势之间的关系。结果证明:通过运用327个多态性SRAP标记来计算11个亲本间的遗传距离,其范围在0.162~0.486,且平均为0.291。亲本间SRAP标记的遗传距离与F_1杂种表现既有相关显著,也有相关不显著;与各农艺性状杂种优势相关达到极显著、显著相关或不显著相关水平,表明SRAP分子标记遗传距离在一定程度上能反映出农艺性状杂种优势的强弱,但其相关程度还不能准确地预测杂种优势。
To explore the feasibility of predicting F_1 heterosis with Raphanus sativus parental genetic distance by using SRAP molecular markers, and to provide references for trait improvement of Raphanus sativus parents and selection of F_1 heterosis, 30 hybrid combinations were assembled by incomplete doublecrossing between 11 glucosinolate-resistant Raphanus sativus parental materials with different agronomic traits. SRAP molecular markers were used to analyze the genetic distance of parents and its correlation relationship with heterosis of agronomic characters. The results showed that the genetic distance range between 11 parents was from 0.162 to 0.486, and the average was 0.291 by using 327 polymorphic SRAP loci. The genetic distance of SRAP markers between parents and F1 hybrids were both significantly correlated and not significantly related; they were extremely significantly correlated or significantly correlated or not significantly correlated with the heterosis of agronomic characters. It was indicated that the genetic distance of SRAP markers could somewhat reflect the strength of heterosis of agronomic characters, but its correlation could not predict the heterosis accurately.
To explore the feasibility of predicting F_1 heterosis with Raphanus sativus parental genetic distance by using SRAP molecular markers, and to provide references for trait improvement of Raphanus sativus parents and selection of F_1 heterosis, 30 hybrid combinations were assembled by incomplete doublecrossing between 11 glucosinolate-resistant Raphanus sativus parental materials with different agronomic traits. SRAP molecular markers were used to analyze the genetic distance of parents and its correlation relationship with heterosis of agronomic characters. The results showed that the genetic distance range between 11 parents was from 0.162 to 0.486, and the average was 0.291 by using 327 polymorphic SRAP loci. The genetic distance of SRAP markers between parents and F1 hybrids were both significantly correlated and not significantly related; they were extremely significantly correlated or significantly correlated or not significantly correlated with the heterosis of agronomic characters. It was indicated that the genetic distance of SRAP markers could somewhat reflect the strength of heterosis of agronomic characters, but its correlation could not predict the heterosis accurately.
引文
[1]袁力行,傅骏骅,刘新芝,等.利用分子标记预测玉米杂种优势的研究[J].中国农业科学,2000,33(6):6-12.
[2]MAKUMBI D,BETRAN J F,BANZIGER M,et al.Combining ability,heterosis and genetic diversity in tropical maize(Zea mays L.)under stress and nonstress conditions[J].Euphytica,2011,180(2):143-162.
[3]BETRAN F J,RIBAUT J M,BECK D,e t a l.Genetic diversity,specific combining ability,and heterosis in tropical maize under stress and nonstress environments[J].Crop Science,2003,43(3):797-806.
[4]KRYSTKOWIAK K,ADAMSKI T,SURMA M,et al.Relationship between phenotypic and genetic diversity of parental genotypes and the specific combining ability and heterosis effects in wheat(Trilicum aeslivum L.)[J].Euphytica,2009,165(3):419-434.
[5]BENIN G,MATEI G,OLIVERIRA A C,et a l.Relationship between four measures of genetic distance and breeding behavior in spring wheat[J].Genetics and Molecular Research,2012,11(3):2390-2400.
[6]张涛,倪先林,蒋开锋,等.水稻功能基因标记遗传距离与杂种优势的相关性研究[J].中国水稻科学,2009,23(6):567-572.
[7]SAGHAI MAROOF M A,YANG G P,ZHANG Q.Correlation between molecular marker distance and hybrid performance in U.S.southern long grain rice[J].Crop Science,1997,37(1):145-150.
[8]赵庆勇,朱镇,张亚东,等.SSR标记遗传距离与粳稻杂种优势的相关性分析[J].中国水稻科学,2009,23(2):141-147.
[9]武耀廷,张天真,朱协飞,等.陆地棉遗传距离与杂种F1、F2产量及杂种优势的相关分析[J].中国农业科学,2002,35(1):22-28.
[10]姚艳梅,聂平,杜德志.不同生态类型甘蓝型油菜的SRAP分析及其遗传距离与杂种优势的关系[J].西北农业学报,2012,21(1):80-87.
[11]LI G,QUIROS C R.Sequence-related amplified polymorphism(SRAP),a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica[J].Theoretical and Applied Genetics,2001,103(2-3):455-461.
[12]张冬菊,李世超,吴鹏夫,等.基于表型和SRAP标记的切花菊品种遗传多样性分析[J].园艺学报,2014,41(1):118-130.
[13]李秀,徐坤,巩彪.生姜种质遗传多样性和亲缘关系的SRAP分析[J].中国农业科学,2014,47(4):718-726.
[14]蔡元保,杨祥燕,陈豪军,等.SRAP结合SCoT标记分析番木瓜种质的遗传多样性[J].植物遗传资源学报,2014,15(2):292-298.
[15]朱献文.萝卜品种遗传多样性的SRAP标记分析[C]//中国农学会.2005植物分子育种国际学术研讨会论文集,2005.
[16]周娜.萝卜种质资源指纹图谱RAPD和SRAP分析[C]//中国园艺学会.中国园艺学会2012年学术年会论文摘要集.中国园艺学会,2012.
[17]刘哲,许园园,苏小俊.萝卜抽薹相关SRAP分子标记筛选与分析[J].江苏农业科学,2016,44(8):74-76.
[18]任喜波,魏毓棠.萝卜主要性状与产量性状间的灰色关联度分析[J].沈阳农业大学学报,2007,38(4):598-601.
[19]ZHANG G Q,QI J M,ZHANG X C,et al.A Genetic linkage map of kenaf(Hibiscus cannabinus L.)based on SRAP,ISSR and RAPD markers[J].Agricultural Sciences in China,2011,10(9):1346-1353.
[20]缪体云,薄天岳,陈锦秀,等.甘蓝种质资源遗传多样性的SRAP分析[J].分子植物育种,2010,8(1):94-98.
[21]徐美兰,金正勋,李晓光,等.7个粳稻SSR和SRAP分子标记遗传距离比较及其与产量性状杂种优势的关系[J].分子植物育种,2009,7(6):1084-1092.
[22]LEE M,GODSHALK K,LAMKEY K R,e t a l.Associat ion of restriction fragment length polymorph isms among maize inbreds with agronomic performance of their of crosses[J].Crop Science,1989,29(4):1067-1071.
[23]SMITH O S,SMITH J S C,BOWEN S L,et a l.Similarities among a group of elite maize inbreds as measured by pedigree,F1 grain yield,grain yield,heterosis,and RFLPs[J].Theoretical and Applied Genetics,1990,80(6):833-840.
[24]廖伏明,周坤炉,阳和华,等.杂交水稻亲本遗传差异及其与杂种优势关系[J].中国水稻科学,1998,12(4):193-199.
[25]张涛,韩磊,徐建第,等.杂交香稻亲本遗传距离与产量杂种优势的相关性研究[J].中国农业科学,2006,39(4):831-835.
[2]MAKUMBI D,BETRAN J F,BANZIGER M,et al.Combining ability,heterosis and genetic diversity in tropical maize(Zea mays L.)under stress and nonstress conditions[J].Euphytica,2011,180(2):143-162.
[3]BETRAN F J,RIBAUT J M,BECK D,e t a l.Genetic diversity,specific combining ability,and heterosis in tropical maize under stress and nonstress environments[J].Crop Science,2003,43(3):797-806.
[4]KRYSTKOWIAK K,ADAMSKI T,SURMA M,et al.Relationship between phenotypic and genetic diversity of parental genotypes and the specific combining ability and heterosis effects in wheat(Trilicum aeslivum L.)[J].Euphytica,2009,165(3):419-434.
[5]BENIN G,MATEI G,OLIVERIRA A C,et a l.Relationship between four measures of genetic distance and breeding behavior in spring wheat[J].Genetics and Molecular Research,2012,11(3):2390-2400.
[6]张涛,倪先林,蒋开锋,等.水稻功能基因标记遗传距离与杂种优势的相关性研究[J].中国水稻科学,2009,23(6):567-572.
[7]SAGHAI MAROOF M A,YANG G P,ZHANG Q.Correlation between molecular marker distance and hybrid performance in U.S.southern long grain rice[J].Crop Science,1997,37(1):145-150.
[8]赵庆勇,朱镇,张亚东,等.SSR标记遗传距离与粳稻杂种优势的相关性分析[J].中国水稻科学,2009,23(2):141-147.
[9]武耀廷,张天真,朱协飞,等.陆地棉遗传距离与杂种F1、F2产量及杂种优势的相关分析[J].中国农业科学,2002,35(1):22-28.
[10]姚艳梅,聂平,杜德志.不同生态类型甘蓝型油菜的SRAP分析及其遗传距离与杂种优势的关系[J].西北农业学报,2012,21(1):80-87.
[11]LI G,QUIROS C R.Sequence-related amplified polymorphism(SRAP),a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica[J].Theoretical and Applied Genetics,2001,103(2-3):455-461.
[12]张冬菊,李世超,吴鹏夫,等.基于表型和SRAP标记的切花菊品种遗传多样性分析[J].园艺学报,2014,41(1):118-130.
[13]李秀,徐坤,巩彪.生姜种质遗传多样性和亲缘关系的SRAP分析[J].中国农业科学,2014,47(4):718-726.
[14]蔡元保,杨祥燕,陈豪军,等.SRAP结合SCoT标记分析番木瓜种质的遗传多样性[J].植物遗传资源学报,2014,15(2):292-298.
[15]朱献文.萝卜品种遗传多样性的SRAP标记分析[C]//中国农学会.2005植物分子育种国际学术研讨会论文集,2005.
[16]周娜.萝卜种质资源指纹图谱RAPD和SRAP分析[C]//中国园艺学会.中国园艺学会2012年学术年会论文摘要集.中国园艺学会,2012.
[17]刘哲,许园园,苏小俊.萝卜抽薹相关SRAP分子标记筛选与分析[J].江苏农业科学,2016,44(8):74-76.
[18]任喜波,魏毓棠.萝卜主要性状与产量性状间的灰色关联度分析[J].沈阳农业大学学报,2007,38(4):598-601.
[19]ZHANG G Q,QI J M,ZHANG X C,et al.A Genetic linkage map of kenaf(Hibiscus cannabinus L.)based on SRAP,ISSR and RAPD markers[J].Agricultural Sciences in China,2011,10(9):1346-1353.
[20]缪体云,薄天岳,陈锦秀,等.甘蓝种质资源遗传多样性的SRAP分析[J].分子植物育种,2010,8(1):94-98.
[21]徐美兰,金正勋,李晓光,等.7个粳稻SSR和SRAP分子标记遗传距离比较及其与产量性状杂种优势的关系[J].分子植物育种,2009,7(6):1084-1092.
[22]LEE M,GODSHALK K,LAMKEY K R,e t a l.Associat ion of restriction fragment length polymorph isms among maize inbreds with agronomic performance of their of crosses[J].Crop Science,1989,29(4):1067-1071.
[23]SMITH O S,SMITH J S C,BOWEN S L,et a l.Similarities among a group of elite maize inbreds as measured by pedigree,F1 grain yield,grain yield,heterosis,and RFLPs[J].Theoretical and Applied Genetics,1990,80(6):833-840.
[24]廖伏明,周坤炉,阳和华,等.杂交水稻亲本遗传差异及其与杂种优势关系[J].中国水稻科学,1998,12(4):193-199.
[25]张涛,韩磊,徐建第,等.杂交香稻亲本遗传距离与产量杂种优势的相关性研究[J].中国农业科学,2006,39(4):831-835.