油菜EIN3/EIL基因家族的鉴定及结构分析
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摘要
Ethylene-insensitive3(EIN3)和EIN3-like(EIL)蛋白是乙烯信号转导途径中重要的核转录因子。EIN3/EIL基因家族在高等植物中分布广泛,在植物的生长、发育等多个过程中有重要的作用。为了揭示油菜EIN3/EIL基因家族的生物学信息,利用生物信息学的方法鉴定了油菜EIN3/EIL基因家族的成员,并从理化性质、亚细胞定位、跨膜结构、二级结构等几个方面对油菜EIN3/EIL基因家族成员进行了预测和分析。这些研究结果有助于进一步研究油菜EIN3/EIL基因家族成员的功能,同时在生产实践中也具有一定的应用价值。
Ethylene-insensitive3(EIN3) and EIN3-like(EIL) proteins are important nuclear transcription factors in ethylene signal transduction pathways. EIN3/EIL gene family is widely distributed in higher plants, and plays an important role in in many processes such as plant growth and development. To reveal biological information of EIN3/EIL gene family in Brassica napus, the members of EIN3/EIL gene family in Brassica napus were identified by using the method of bioinformatics, and the members of EIN3/EIL gene family were predicted and analyzed in terms of physicochemical properties, subcellular localization, transmembrane structure and secondary structure. The results would be beneficial for further research on the function of EIN3/EIL gene family members in Brassica napus, and would also have a certain application value in production practice.
Ethylene-insensitive3(EIN3) and EIN3-like(EIL) proteins are important nuclear transcription factors in ethylene signal transduction pathways. EIN3/EIL gene family is widely distributed in higher plants, and plays an important role in in many processes such as plant growth and development. To reveal biological information of EIN3/EIL gene family in Brassica napus, the members of EIN3/EIL gene family in Brassica napus were identified by using the method of bioinformatics, and the members of EIN3/EIL gene family were predicted and analyzed in terms of physicochemical properties, subcellular localization, transmembrane structure and secondary structure. The results would be beneficial for further research on the function of EIN3/EIL gene family members in Brassica napus, and would also have a certain application value in production practice.
引文
Bleecker A.B.,and Kende H.,2000,Ethylene:a gaseous signa molecule in plants,Annual Review Cell and Developmenta Biology,16:1-18
Cao W.H.,Liu J.,He X.J.,Mu R.L.,Zhou H.L.,Chen S.Y.,and Zhang J.S.,2006,Modulation of ethylene responses affects plant salt-stress responses,Plant Physiology,143(2):707-719
Chao Q.M.,Rothenberg M.,Solano R.,Roman G.,Terzaghi W.and Ecker J.R.,1997,Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHY-LENE-INSENSITIVE3 and related proteins,Cell,89(7):1133-1144
Duan X.H.,Sun L.H.,Guo X.R.,Wang H.N.,and Zu Y.G.,2009,Morphological difference of Arabidopsis thaliana ethylene mutants affected by drought stress,Zhiwu Yanjiu(Bulletin of Botanical Research),29(1):39-42(段喜华,孙莲慧,郭晓瑞,王化楠,祖元刚,2009,拟南芥乙烯突变体在干旱胁迫下的形态学差异,植物研究,29(1):39-42)
Gao S.,Xu D.X.,Chen Z.S.,Xie X.X.,Zhong K.Q.,Lin B.Y.,and Wen Q.F.,2015,Cloning and expression analysis of transcription factor McEIL2 in Momordica charantia L.,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(6):1309-1317(高山,许端祥,陈中钐,谢鑫鑫,钟开勤,林碧英,温庆放,2015,苦瓜转录因子McEIL2的克隆及其表达分析,分子植物育种,13(6):1309-1317)
Hiraga S.,Sasaki K.,Hibi T.,Yoshida H.,Uchida E.,Kosugi S.,Kato T.,Mie T.,Ito H.,Katou S.,Seo S.,Matsui H.,Ohashi Y.,and Mitsuhara I.,2009,Involvement of two rice ETHYLENEINSENSITIVE3-LIKE genes in wound signaling,Molecular Genetics and Genomics,282(5):517-529
Kosugi S.,and Ohashi Y.,2000,Cloning and DNA-binding properties of a tobacco ethylene-Insensitive3(EIN3)homolog,Nucleic Acids Research,28(4):960-967
Li J.,2007,Construction of Le-EIN3 expression vectors and purification of the recombinant protein,Thesis for M.S.,Chongqing University,Supervisor:Li G.Z.,pp.3-4(李季,2007,番茄EIN3蛋白质表达载体的构建及表达纯化,硕士学位论文,重庆大学,导师:李国正,pp.3-4)
Li W.Y.,Ma M.D.,and Guo H.W.,2013,Advances in the action of plant hormone ethylene,Zhongguo Kexue:Shengming Kexue(Scientia Sinica Vitae),43(10):854-863(李文阳,马梦迪,郭红卫,2013,植物激素乙烯作用机制的最新进展中国科学:生命科学,43(10):854-863)
Lin Z.F.,Zhong S.L.,and Grierson D.,2009,Recent advances in ethylene research,Journal of Experimental Botany,60(12)3311-3336
Mao C.Z.,Wang S.M.,Jia Q.J.,and Wu P.,2006,OsEIL1,a rice homolog of the Arabidopsis EIN3 regulates the ethylene response as a positive component,Plant Molecular Biology61(1-2):141-152
Maruyama-Nakashita A.,Nakamura Y.,Tohge T.,Saito K.,and Takahashi H.,2006,Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism,The Plant Cell,18(11):3235-3251
Ma Y.,Shen S.H.,Bade R.G.,and Hasi A.G.L.,2014,Genome-wide identification and phylogenetic analysis of the EIN3/EILs gene family in melons,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(1):105-112(马勇莘少红,巴德仁贵,哈斯阿古拉,2014,甜瓜EIN3/EILs基因家族全基因组鉴定与进化分析,基因组学与应用生物学,33(1):105-112)
Rieu I.,Mariani C.,and Weterings K.,2003,Expression analysis of five tobacco EIN3 family members in relation to tissue specific ethylene responses,Journal of Exprimental Botany54(391):2239-2244
Solano R.,Stepanova A.,Chao Q.M.,and Ecker J.R.,1998,Nuclear events in ethylene signaling:a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1,Genes&Development,12(23):3703-3714
Sun Y.W.,2016,Phylogeny of the EIN3/EIL genes family,Thesis for M.S.,Zhejiang A&F University,Supervisor:Huang Y.J.,pp.8-9(孙义伟,2016,EIN3/EIL家族的系统进化,硕士学位论文,浙江农林大学,导师:黄有军,pp.8-9)
Tieman D.M.,Ciardi J.A.,Taylor M.G.,and Klee H.J.,2001
Members of the tomato LeEIL(EIN3-like)gene family are functionally redundant and regulate ethylene responses throughout plant development,The Plant Journal,26(1):47-58
Wang P.,Zhang B.,Li X.,Xu C.J.,Yin X.R.,Shan L.L.,Ferguson I.,and Chen K.S.,2010,Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit,Journal of Experimental Botany,61(1):179-190
Wang Y.N.,Liu C.,Li K.X.,Sun F.F.,Hu H.Z.,Li X.,Zhao Y.K.,Han C.Y.,Zhang W.S.,Duan Y.,Liu M.Y.,and Li X.,2007,Arabidopsis EIN2 modulates stress response through abscisic acid response pathway,Plant Molecular Biology,64(6):633-644
Wang Y.J.,Zhang C.,Wang X.Q.,and Dong L.,2012,Research advances of EIN3/EIL transcription factors in higher plants,Shengwu Jishu Tongbao(Biotechnology Bulletin),29(3):1-8(王彦杰,张超,王晓庆,董丽,2012,高等植物EIN3/EILs转录因子研究进展,生物技术通报,29(3):1-8)
Xu Q.,Yin X.R.,and Chen K.S.,2014,EIN3/EIL and AP2/ERFare involved in transcriptional regulation on fruit quality,Yuanyi Xuebao(Acta Horticulturae Sinica),41(9):1913-1923(徐倩,殷学仁,陈昆松,2014,基于乙烯受体下游转录因子的果实品质调控机制研究进展,园艺学报,41(9):1913-1923)
Yin X.R.,Allan A.C.,Chen K.S.,and Ferguson I.B.,2010,Kiwifruit EIL and ERF genes involved in regulating fruit ripening,Plant Physiology,153(3):1280-1292
Yokotani N.,Tamura S.,Nakano R.,Inaba A.,and Kubo Y.,2003,Characterization of a novel tomato EIN3-like gene(Le EIL4),Journal of Experimental Botany,54(393):2775-2776
Zhang X.,Li X.L.,Guo W.T.,Zhang P.F.,and Zhang W.,2017,Sequence analysis of EIN3/EIL gene family and cloning of GhEIL3 in cotton,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),37(3):445-452(张析,李潇玲,郭文婷,张鹏飞,张薇,2017,棉花EIN3/EIL家族基因序列分析及GhEIL3基因克隆,西北植物学报,37(3):445-452)
Cao W.H.,Liu J.,He X.J.,Mu R.L.,Zhou H.L.,Chen S.Y.,and Zhang J.S.,2006,Modulation of ethylene responses affects plant salt-stress responses,Plant Physiology,143(2):707-719
Chao Q.M.,Rothenberg M.,Solano R.,Roman G.,Terzaghi W.and Ecker J.R.,1997,Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHY-LENE-INSENSITIVE3 and related proteins,Cell,89(7):1133-1144
Duan X.H.,Sun L.H.,Guo X.R.,Wang H.N.,and Zu Y.G.,2009,Morphological difference of Arabidopsis thaliana ethylene mutants affected by drought stress,Zhiwu Yanjiu(Bulletin of Botanical Research),29(1):39-42(段喜华,孙莲慧,郭晓瑞,王化楠,祖元刚,2009,拟南芥乙烯突变体在干旱胁迫下的形态学差异,植物研究,29(1):39-42)
Gao S.,Xu D.X.,Chen Z.S.,Xie X.X.,Zhong K.Q.,Lin B.Y.,and Wen Q.F.,2015,Cloning and expression analysis of transcription factor McEIL2 in Momordica charantia L.,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(6):1309-1317(高山,许端祥,陈中钐,谢鑫鑫,钟开勤,林碧英,温庆放,2015,苦瓜转录因子McEIL2的克隆及其表达分析,分子植物育种,13(6):1309-1317)
Hiraga S.,Sasaki K.,Hibi T.,Yoshida H.,Uchida E.,Kosugi S.,Kato T.,Mie T.,Ito H.,Katou S.,Seo S.,Matsui H.,Ohashi Y.,and Mitsuhara I.,2009,Involvement of two rice ETHYLENEINSENSITIVE3-LIKE genes in wound signaling,Molecular Genetics and Genomics,282(5):517-529
Kosugi S.,and Ohashi Y.,2000,Cloning and DNA-binding properties of a tobacco ethylene-Insensitive3(EIN3)homolog,Nucleic Acids Research,28(4):960-967
Li J.,2007,Construction of Le-EIN3 expression vectors and purification of the recombinant protein,Thesis for M.S.,Chongqing University,Supervisor:Li G.Z.,pp.3-4(李季,2007,番茄EIN3蛋白质表达载体的构建及表达纯化,硕士学位论文,重庆大学,导师:李国正,pp.3-4)
Li W.Y.,Ma M.D.,and Guo H.W.,2013,Advances in the action of plant hormone ethylene,Zhongguo Kexue:Shengming Kexue(Scientia Sinica Vitae),43(10):854-863(李文阳,马梦迪,郭红卫,2013,植物激素乙烯作用机制的最新进展中国科学:生命科学,43(10):854-863)
Lin Z.F.,Zhong S.L.,and Grierson D.,2009,Recent advances in ethylene research,Journal of Experimental Botany,60(12)3311-3336
Mao C.Z.,Wang S.M.,Jia Q.J.,and Wu P.,2006,OsEIL1,a rice homolog of the Arabidopsis EIN3 regulates the ethylene response as a positive component,Plant Molecular Biology61(1-2):141-152
Maruyama-Nakashita A.,Nakamura Y.,Tohge T.,Saito K.,and Takahashi H.,2006,Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism,The Plant Cell,18(11):3235-3251
Ma Y.,Shen S.H.,Bade R.G.,and Hasi A.G.L.,2014,Genome-wide identification and phylogenetic analysis of the EIN3/EILs gene family in melons,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(1):105-112(马勇莘少红,巴德仁贵,哈斯阿古拉,2014,甜瓜EIN3/EILs基因家族全基因组鉴定与进化分析,基因组学与应用生物学,33(1):105-112)
Rieu I.,Mariani C.,and Weterings K.,2003,Expression analysis of five tobacco EIN3 family members in relation to tissue specific ethylene responses,Journal of Exprimental Botany54(391):2239-2244
Solano R.,Stepanova A.,Chao Q.M.,and Ecker J.R.,1998,Nuclear events in ethylene signaling:a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1,Genes&Development,12(23):3703-3714
Sun Y.W.,2016,Phylogeny of the EIN3/EIL genes family,Thesis for M.S.,Zhejiang A&F University,Supervisor:Huang Y.J.,pp.8-9(孙义伟,2016,EIN3/EIL家族的系统进化,硕士学位论文,浙江农林大学,导师:黄有军,pp.8-9)
Tieman D.M.,Ciardi J.A.,Taylor M.G.,and Klee H.J.,2001
Members of the tomato LeEIL(EIN3-like)gene family are functionally redundant and regulate ethylene responses throughout plant development,The Plant Journal,26(1):47-58
Wang P.,Zhang B.,Li X.,Xu C.J.,Yin X.R.,Shan L.L.,Ferguson I.,and Chen K.S.,2010,Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit,Journal of Experimental Botany,61(1):179-190
Wang Y.N.,Liu C.,Li K.X.,Sun F.F.,Hu H.Z.,Li X.,Zhao Y.K.,Han C.Y.,Zhang W.S.,Duan Y.,Liu M.Y.,and Li X.,2007,Arabidopsis EIN2 modulates stress response through abscisic acid response pathway,Plant Molecular Biology,64(6):633-644
Wang Y.J.,Zhang C.,Wang X.Q.,and Dong L.,2012,Research advances of EIN3/EIL transcription factors in higher plants,Shengwu Jishu Tongbao(Biotechnology Bulletin),29(3):1-8(王彦杰,张超,王晓庆,董丽,2012,高等植物EIN3/EILs转录因子研究进展,生物技术通报,29(3):1-8)
Xu Q.,Yin X.R.,and Chen K.S.,2014,EIN3/EIL and AP2/ERFare involved in transcriptional regulation on fruit quality,Yuanyi Xuebao(Acta Horticulturae Sinica),41(9):1913-1923(徐倩,殷学仁,陈昆松,2014,基于乙烯受体下游转录因子的果实品质调控机制研究进展,园艺学报,41(9):1913-1923)
Yin X.R.,Allan A.C.,Chen K.S.,and Ferguson I.B.,2010,Kiwifruit EIL and ERF genes involved in regulating fruit ripening,Plant Physiology,153(3):1280-1292
Yokotani N.,Tamura S.,Nakano R.,Inaba A.,and Kubo Y.,2003,Characterization of a novel tomato EIN3-like gene(Le EIL4),Journal of Experimental Botany,54(393):2775-2776
Zhang X.,Li X.L.,Guo W.T.,Zhang P.F.,and Zhang W.,2017,Sequence analysis of EIN3/EIL gene family and cloning of GhEIL3 in cotton,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),37(3):445-452(张析,李潇玲,郭文婷,张鹏飞,张薇,2017,棉花EIN3/EIL家族基因序列分析及GhEIL3基因克隆,西北植物学报,37(3):445-452)