北京地区健康鸭肠道大肠杆菌血清型鉴定和耐药性分析
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摘要
为了解北京地区北京鸭肠道大肠杆菌分离株的血清型和耐药情况,从北京市周边地区的散养鸭场采集健康北京鸭的泄殖腔拭子100份,通过细菌分离纯化、PCR鉴定,共分离鉴定出78株大肠杆菌,对其进行分子分群、药敏试验以及血清型检测。结果显示:分离的78株大肠杆菌中A群、B1群、B2群、D群分别占48.72%、10.26%、15.38%和25.64%;有66株分别属于13个血清型,其中O4、O6、O8、O75和O88血清型分离株均大于10%,为优势血清型;分离株对氨苄西林、阿莫西林克拉维酸、氯霉素、恩诺沙星、多西环素、四环素、氟苯尼考等16种抗生素均表现出不同程度的耐药性,对4种以上抗生素耐药的菌株占94.87%,呈现严重的多重耐药性。综上所述,北京地区健康北京鸭肠道内的大肠杆菌分子分群各不相同,多重耐药现象严重,同时血清型众多,应该加强散养户鸭源大肠杆菌耐药性和血清型的监测。
引文
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[15]吴信明,连洪梅,王艳,等.38株鸭源大肠杆菌的分离鉴定与药敏试验[J].中国畜牧兽医,2010,37(10):178-180.
[2]MA J,LIU J H,LV L,et al.Characterization of extended-spectrumβ-lactamase genes found among Escherichia coli isolates from duck and environmental samples obtained on a duck farm[J].Applied and Environmental Microbiology,2012,78(10):3668-3673.
[3]WANG Y,TANG C,YU X,et al.Distribution of serotypes and virulence-associated genes in pathogenic Escherichia coli isolated from ducks[J].Avian Pathology,2010,39(4):297-302.
[4]刘砚涵,陈诗佳音,冯献程,等.北京地区鸭源大肠杆菌耐药表型和Tet aac耐药基因的检测[J].中国兽医杂志,2018,54(9):79-83.
[5]CLERMONTl O,BONACORSI S,BINGEN E.Rapid and simple determination of the Escherichia coli phylogenetic group[J].Applied and Environmental Microbiology,2000,66(10):4555-4558.
[6]严杜建.鸭源大肠杆菌抗O血清制备与同源性测定[J].黑龙江畜牧兽医,2016(22):144-146.
[7]刘传凯,夏庆祥.鸭大肠杆菌病的防治技术要点[J].家禽科学,2019(1):45-46.
[8]郭磊,李开锋,王述柏,等.山东地区鸭源大肠杆菌耐药性分析[J].中国兽医杂志,2018,54(2):87-89.
[9]ABDALLAH W S A.Prevalence,molecular characterization and antibiotic susceptibility of Escherichia coli isolated from duck meat and giblets[J].Journal of Food and Safety,2015,35(3):410-415.
[10]央珍,聂奎.重庆市部分地区鸭源大肠杆菌的O抗原血清型及耐药性[J].西南大学学报(自然科学版),2011,33(8):52-56.
[11]伍莉,陈鹏飞.鸭致病性大肠杆菌的血清型鉴定及敏感药物的临床应用[J].中国兽医杂志,2015,51(12):41-43.
[12]NICOLASCHANOINE M,Blanco J,LEFLINGUIBOUT V,et al.Intercontinental emergence of Escherichia coli clone O25∶H4-ST131 producing CTX-M-15[J].Journal of Antimicrobial Chemotherapy,2008,61(2):273.
[13]张代,赵耀光.鸭致病性大肠杆菌的分离鉴定及药敏试验[J].现代农村科技,2018(10):71-72.
[14]徐振娜,洪伟彬,李永福,等.东莞市屠宰场环境及屠宰猪体内致病性大肠杆菌的分离与鉴定[J].中国兽医杂志,2016,52(3):101-103.
[15]吴信明,连洪梅,王艳,等.38株鸭源大肠杆菌的分离鉴定与药敏试验[J].中国畜牧兽医,2010,37(10):178-180.