持续低产蛋率蛋鸭群主要病毒感染的检测与分析
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摘要
为了明确持续低产蛋率开产蛋鸭群的主要病毒病感染情况,应用已建立的间接ELISA方法和血凝抑制试验对2013年1月至2015年10月采自福建、广东、广西、江西、浙江、江苏和安徽等7省(区)免疫过H5亚型和H9亚型禽流感灭活疫苗但未免疫禽1型副黏病毒病疫苗和禽坦布苏病毒病疫苗,表现为持续低产蛋率的29个开产蛋鸭群的4 737份血清样品进行抗体检测,同时采集蛋鸭卵巢组织进行RT-PCR检测。结果显示,29个开产蛋鸭群中,H5亚型禽流感、H9亚型禽流感、禽1型副黏病毒病和禽坦布苏病毒病抗体的群阳性率分别为100.0%,100.0%,10.3%和100.0%,群内阳性率分别为79.4%~100.0%,82.5%~100.0%,0.0%~13.1%和33.7%~100.0%;蛋鸭卵巢组织中H5亚型禽流感病毒、H9亚型禽流感病毒、禽1型副黏病毒和禽坦布苏病毒的阳性率依次为13.8%(4/29),0.0%(0/0),3.5%(1/29)和86.2%(25/29)。上述结果表明,我国以上7省(区)表现持续低产蛋率的开产蛋鸭群存在禽坦布苏病毒的严重感染,应引起养鸭生产者的高度重视。
In order to evaluate the primary virus infection in persistent low-productivity duck flocks,a total of 4 737 serum samples and ovary samples(3 samples per flock)from 29 egg-laying duck flocks distributed in Fujian,Guangdong,Guangxi,Jiangxi,Zhejiang,Jiangsu,and Anhui provinces from January 2013 to October 2015 were collected for indirected ELISA and RT-PCR detection,respectively.All flocks were immunized with H5 and H9 vaccines but no avian paramyxovirus type 1 and avian Tembusu virus vaccines,which presented persistently low laying rate.The results showed that the antibody positive rates in inter-flock of AIV-H5,AIV-H9,avian paramyxovirus type 1,and avian Tembusu virus infection were 100.0%,100.0%,10.3%,and 100%,respectively,those in intra-flock of 79.4%-100.0%,82.5%-100.0%,0.0%-13.1% and 33.7%-100.0%,respectively.Meanwhile,the flock positive rates of AIV-H5,AIV-H9,avian paramyxovirus type 1 and avian Tembusu virus detected by RT-PCR were 13.8%(4/29),0.0%(0/0),3.5%(1/29),and86.2%(25/29),respectively.The above results proved that avian Tembusu virus infection was widely spread in egg-laying duck flocks and directly related to persistently low-yield in duck flocks.
In order to evaluate the primary virus infection in persistent low-productivity duck flocks,a total of 4 737 serum samples and ovary samples(3 samples per flock)from 29 egg-laying duck flocks distributed in Fujian,Guangdong,Guangxi,Jiangxi,Zhejiang,Jiangsu,and Anhui provinces from January 2013 to October 2015 were collected for indirected ELISA and RT-PCR detection,respectively.All flocks were immunized with H5 and H9 vaccines but no avian paramyxovirus type 1 and avian Tembusu virus vaccines,which presented persistently low laying rate.The results showed that the antibody positive rates in inter-flock of AIV-H5,AIV-H9,avian paramyxovirus type 1,and avian Tembusu virus infection were 100.0%,100.0%,10.3%,and 100%,respectively,those in intra-flock of 79.4%-100.0%,82.5%-100.0%,0.0%-13.1% and 33.7%-100.0%,respectively.Meanwhile,the flock positive rates of AIV-H5,AIV-H9,avian paramyxovirus type 1 and avian Tembusu virus detected by RT-PCR were 13.8%(4/29),0.0%(0/0),3.5%(1/29),and86.2%(25/29),respectively.The above results proved that avian Tembusu virus infection was widely spread in egg-laying duck flocks and directly related to persistently low-yield in duck flocks.
引文
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[23]祖立闯,苗立中,王艳,等.鸭混合感染病例中鸭坦布苏病毒的分离鉴定与E基因序列分析[J].中国兽医学报,2015,35(4):553-557.
[24]张英,赵丹丹,刁有祥,等.坦布苏病毒在种鸭中的垂直传播[J].中国兽医学报,2016,36(11):1847-1852.
[25]赵立媛,李秀丽,刁有祥,等.坦布苏病毒在樱桃谷雏鸭体内的排毒规律及病毒在血液、组织中载量的变化[J].中国兽医学报,2016,36(5):718-722.
[26]李小康,张巫凡,杨静,等.鸭坦布苏病毒HN1株囊膜E蛋白抑制肽的筛选及鉴定[J].中国兽医学报,2016,36(11):1842-1846.
[27]钱晨,汪义娟,宋振华.种鸭产蛋下降的原因分析[J].中国家禽,2006,28(21):526-26.
[28]孟定,何其彬,钱志伟.蛋鸭腺病毒感染的诊治[J].畜禽业,2014(10):80-81.
[2]黄夏.产蛋麻鸭大肠杆菌病的诊疗[J].畜牧与兽医,1997(1):28-29.
[3]司红彬,陈鹏举,宋剑武.一例由大肠杆菌引起种鸭产蛋下降病例的思考[J].黑龙江畜牧兽医,2011(3):97-98.
[4]程龙飞,彭春香,郑腾,等.种番鸭肠炎沙门氏菌的分离鉴定[J].福建畜牧兽医,2007,29(3):24-25.
[5]黄瑜,程龙飞,傅光华,等.鸭流感的临床病型[J].福建畜牧兽医,2006,28(3):54-55.
[6]黄瑜,苏荣茂,傅光华,等.禽坦布苏病毒感染的宿主及临床表现[J].中国兽医杂志,2014,30(11):21-25.
[7]CAO Z,ZHANG C,LIU Y,et al.Tembusu virus in ducks,China[J].Emerg Infect Dis,2011,17(10):1873-1875.
[8]YAN P,ZHAO Y,ZHANG X,et al.An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China[J].Virology,2011,417(1):1-8.
[9]SAIF Y M.禽病学[M].11版.苏敬良,高福,索勋,主译.北京:中国农业出版社,2005:65-98.
[10]黄勇.产蛋麻鸭大肠杆菌病与禽Ⅰ型副粘病毒病混合感染的诊治[J].四川畜牧兽医,2009,36(6):51-52.
[11]陈伯伦.鸭病[M].北京:中国农业出版社,2008:49-50.
[12]施少华,傅光华,万春和,等.检测鸭坦布苏病毒卵黄抗体间接ELISA方法的建立[J].养禽与禽病防治,2012(2):2-4.
[13]中华人民共和国国家质量监督检验检疫总局.SPF鸡微生物学监测第2部分:SPF鸡红细胞凝集抑制试验:GB/T 17999.2-2008[S].北京:中国标准出版社,2009.
[14]中华人民共和国国家质量监督检验检疫总局.H5亚型禽流感病毒荧光RT-PCR检测方法:GB/T19438.2-2004[S].北京:中国标准出版社,2005.
[15]中华人民共和国国家质量监督检验检疫总局.H9亚型禽流感病毒荧光RT-PCR检测方法:GB/T19438.2-2004:[S].北京:中国标准出版社,2005.
[16]施少华,程龙飞,陈红梅,等.水禽源禽1型副黏病毒强毒RT-PCR方法的建立[J].福建农业学报,2009,24(1):11-13.
[17]万春和,施少华,程龙飞,等.鸭出血性卵巢炎病毒RT-PCR检测方法的建立[J].福建农业学报,2011,26(1):10-12.
[18]万春和,施少华,程龙飞,等.一种引起种(蛋)鸭产蛋骤降新病毒的分离与初步鉴定[J].福建农业学报,2010,25(6):663-666.
[19]胡旭东,路浩,刘培培,等.我国发现的一种引起鸭产蛋下降综合征的新型黄病毒[J].中国兽医杂志,2011,47(7):43-47.
[20]SU J,LI S,HU X,et al.Duck egg-drop syndrome caused by BYD virus,a new Tembusu-related flavivirus[J].PLoS One,2011,6(3):e18106.
[21]王红琳,商雨,温国元.一株鸭源副黏病毒的分离鉴定[J].中国家禽,2011,33(9):50-51.
[22]谭刘刚,鲁梅,朱凤株,等.1株中国北方鸭H4N6亚型禽流感病毒的分子特征[J].中国兽医学报,2015,35(2):207-211.
[23]祖立闯,苗立中,王艳,等.鸭混合感染病例中鸭坦布苏病毒的分离鉴定与E基因序列分析[J].中国兽医学报,2015,35(4):553-557.
[24]张英,赵丹丹,刁有祥,等.坦布苏病毒在种鸭中的垂直传播[J].中国兽医学报,2016,36(11):1847-1852.
[25]赵立媛,李秀丽,刁有祥,等.坦布苏病毒在樱桃谷雏鸭体内的排毒规律及病毒在血液、组织中载量的变化[J].中国兽医学报,2016,36(5):718-722.
[26]李小康,张巫凡,杨静,等.鸭坦布苏病毒HN1株囊膜E蛋白抑制肽的筛选及鉴定[J].中国兽医学报,2016,36(11):1842-1846.
[27]钱晨,汪义娟,宋振华.种鸭产蛋下降的原因分析[J].中国家禽,2006,28(21):526-26.
[28]孟定,何其彬,钱志伟.蛋鸭腺病毒感染的诊治[J].畜禽业,2014(10):80-81.