摘要
2010年,从山东省水禽市场健康鸭体内分离鉴定了1株H4N6禽流感病毒,命名为A/duck/Shandong/1/2010(DK/SD/1/2010)。对该病毒进行全基因组测序,并与Gen Bank中相关序列进行遗传和进化分析。结果表明,DK/SD/1/2010的7个基因(PB2,PB1,PA,HA,NP,M和NS)来源于欧亚系,NA基因来自于欧亚系和北美系。DK/SD/1/2010 HA裂解位点氨基酸序列(PKKASR↓GLF)和低致病性禽流感的特征一致。动物接种试验表明该病毒不能在鸡和小鼠中复制。推测DK/SD/1/2010可能是不同来源的流感病毒在鸭体内重组和演变形成的重组病毒,该病毒对鸡和鸭无明显致病性。
In 2010,an H4N6 avian influenza virus( AIV) was isolated and identified from healthy ducks in a waterfowl market in Shandong province in northern China. This virus was named A / duck / Shandong /1 /2010( H4N6)( DK / SD /1 /2010 here after). The gene sequence of the virus was determined,and genetic and evolutionary analysis were conducted by combining related sequences in Gen Bank. Results indicated that seven genes of DK / SD /1 /2010( PB2,PB1,PA,HA,NP,M and NS) originated from the Eurasian lineage. Another gene,the NA gene,originated from both the Eurasian and the North American lineages. The amino acid sequence near the cleavage site of DK / SD /1 /2010 HA( PKKASR↓GLF) corresponded to the characteristics of AIV of low pathogenicity. Animal inoculation tests showed that the virus can not replicate in chickens and mice. Therefore,DK / SD /1 /2010 may be a recombinant virus formed by influenza virus genes from different sources through complicated restructuring and evolution in ducks,and is avirulent to chickens and ducks.
引文
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