1株中国北方鸭H4N6亚型禽流感病毒的分子特征
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摘要
2010年,从山东省水禽市场健康鸭体内分离鉴定了1株H4N6禽流感病毒,命名为A/duck/Shandong/1/2010(DK/SD/1/2010)。对该病毒进行全基因组测序,并与Gen Bank中相关序列进行遗传和进化分析。结果表明,DK/SD/1/2010的7个基因(PB2,PB1,PA,HA,NP,M和NS)来源于欧亚系,NA基因来自于欧亚系和北美系。DK/SD/1/2010 HA裂解位点氨基酸序列(PKKASR↓GLF)和低致病性禽流感的特征一致。动物接种试验表明该病毒不能在鸡和小鼠中复制。推测DK/SD/1/2010可能是不同来源的流感病毒在鸭体内重组和演变形成的重组病毒,该病毒对鸡和鸭无明显致病性。
In 2010,an H4N6 avian influenza virus( AIV) was isolated and identified from healthy ducks in a waterfowl market in Shandong province in northern China. This virus was named A / duck / Shandong /1 /2010( H4N6)( DK / SD /1 /2010 here after). The gene sequence of the virus was determined,and genetic and evolutionary analysis were conducted by combining related sequences in Gen Bank. Results indicated that seven genes of DK / SD /1 /2010( PB2,PB1,PA,HA,NP,M and NS) originated from the Eurasian lineage. Another gene,the NA gene,originated from both the Eurasian and the North American lineages. The amino acid sequence near the cleavage site of DK / SD /1 /2010 HA( PKKASR↓GLF) corresponded to the characteristics of AIV of low pathogenicity. Animal inoculation tests showed that the virus can not replicate in chickens and mice. Therefore,DK / SD /1 /2010 may be a recombinant virus formed by influenza virus genes from different sources through complicated restructuring and evolution in ducks,and is avirulent to chickens and ducks.
In 2010,an H4N6 avian influenza virus( AIV) was isolated and identified from healthy ducks in a waterfowl market in Shandong province in northern China. This virus was named A / duck / Shandong /1 /2010( H4N6)( DK / SD /1 /2010 here after). The gene sequence of the virus was determined,and genetic and evolutionary analysis were conducted by combining related sequences in Gen Bank. Results indicated that seven genes of DK / SD /1 /2010( PB2,PB1,PA,HA,NP,M and NS) originated from the Eurasian lineage. Another gene,the NA gene,originated from both the Eurasian and the North American lineages. The amino acid sequence near the cleavage site of DK / SD /1 /2010 HA( PKKASR↓GLF) corresponded to the characteristics of AIV of low pathogenicity. Animal inoculation tests showed that the virus can not replicate in chickens and mice. Therefore,DK / SD /1 /2010 may be a recombinant virus formed by influenza virus genes from different sources through complicated restructuring and evolution in ducks,and is avirulent to chickens and ducks.
引文
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[2]Iamnikova S S,Kurinov G V,Lomakina N F,et al.Infection of pigs with influenza A/H4 and A/H5 viruses isolated from wild b irds on the territory of Russia[J].Vopr Virusol,2008,53:30-34.
[3]Senne D A.Avian influenza in the Western Hemisphere including the Pacific Islands and Australia[J].Avian Dis,2003,47:798-805.
[4]Ninomiya A,Takada A,Okazaki K,et al.Seroepidemiological evidence of avian H4,H5,and H9 influenza A virus transmission to pigs in south-eastern China[J].Vet Microbiol,2002,88:107-114.
[5]Chen H,Deng G,Li Z,et al.The evolution of H5N1 influenza viruses in ducks in southern China Proc[J].Natl Acad Sci USA,2004,101(28):10452-10457.
[6]Lee H J,Kwon J S,Lee D H,et al.Continuing evolution and interspecies transmission of influenza viruses in live bird markets in Korea[J].Avian Dis,2010,54:738-748.
[7]Pasick J,Berhane Y,Kehler H,et al.Survey of influenza A viruses circulating in wild birds in Canada 2005 to2007[J].Avian Dis,2010,54:440-445.
[8]Kayali G,Ortiz E J,Chorazy M L,et al.Evidence of previous avian influenzainfection among US turkey workers[J].Zoonoses Public Health,2010,57:265-272.
[9]Hanson B A,Stallknecht D E,Swayne D E,et al.Avian influenza viruses in Minnesota ducks during 1998-2000[J].Avian Dis,2003,47:867-871.
[10]Cheng M C,Lee M S,Ho Y H,et al.Avian influenza monitoring in migrating birds in Taiwan during 1998-2007[J].Avian Dis,2010,54:109-114.
[11]Webster R G,Bean W J,Gorman O T,et al.Evolution and ecology of influenza A viruses[J].Microbiol Rev,1992,56:152-79.
[12]Robertson J S.Sequence analysis of the haemagglutinin of A/Taiwan/1/86,a new variant of human influenza A(H1N1)virus[J].Gen Virol,1987,68(4):1205-1208.