溶剂效应与磁场强化协同作用结晶纯化植物甾醇
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摘要
以大豆油脱臭馏出物为原料,经过酯化预处理分离出粗甾醇,以乙酸乙酯与甲醇水的混合液为结晶溶剂,与磁场协同作用进行单因素试验和正交试验。确定的最佳工艺条件为:料液比1︰30 (g/mL)、甲醇水用量6%、磁场强度0.6 T,在磁场作用下,测得结晶时间为42 min,养晶1 h得到纯度为98.43%、回收率为91.61%的精制甾醇。
The crude sterols were separated from deodorizer distillate of soybean oil by esterification pretreatment, and then single factor experiment and orthogonal experiment were carried out under the magnetic field and with the mixture of ethyl acetate and methanol-water as crystal solvent. The optimum technological condition was the ratio of solid to liquid 1︰30(g/m L), the amount of methanol-water 6% and the magnetic field strength 0.6 T. The crystallization time was observed to be 42 min under the action of magnetic field. Under this condition, the purity of sterol was 98.43% and the recovery rate of sterol was 91.61%.
The crude sterols were separated from deodorizer distillate of soybean oil by esterification pretreatment, and then single factor experiment and orthogonal experiment were carried out under the magnetic field and with the mixture of ethyl acetate and methanol-water as crystal solvent. The optimum technological condition was the ratio of solid to liquid 1︰30(g/m L), the amount of methanol-water 6% and the magnetic field strength 0.6 T. The crystallization time was observed to be 42 min under the action of magnetic field. Under this condition, the purity of sterol was 98.43% and the recovery rate of sterol was 91.61%.
引文
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[24]吕双双,任婷婷,李书国.玉米胚芽油脱臭馏出物中植物甾醇和VE同时提取的研究[J].食品科技,2014(6):182-187.
[25]张海燕,张杰,尚富德.玉米须中高含量植物甾醇的提纯工艺[J].食品工业科技,2017,38(3):192-195.
[26]李俊杰.高γ-谷维素、β-谷甾醇米糠油的提取及精炼工艺研究[D].长沙:湖南农业大学,2016.
[27]万建春,姜波.结晶法分离纯化大豆甾醇中β-谷甾醇和豆甾醇单体[J].食品科技,2008,33(8):127-131.
[2]杨振强,谢文磊,李海涛,等.植物甾醇的开发与应用研究进展[J].粮油加工与食品机械,2006(1):53-56.
[3]左玉.植物甾醇研究与应用[J].粮食与油脂,2012,25(7):1-4.
[4]KENDRICK M.The great cholesterol con[M].Lodon:John-Blake,2008.
[5]NES W D.Biosynthesis of cholesterol and other sterols[J].Chem Rev,2011,111:6423-6451.
[6]王稳航.植物甾醇及其在食品中的应用[J].西部粮油科技,2002,27(6):41-44.
[7]张莉华,许新德,邵斌,等.油脂加工副产物--脱臭馏出物的综合利用[J].中国食品添加剂,2015(1):157-164.
[8]鲁海龙,史宣明,张旋,等.植物甾醇制取及应用研究进展[J].中国油脂,2017,42(10):134-137.
[9]韩军花.植物甾醇的性质、功能及应用[J].国外医学卫生学分册,2001,28(5):285-291.
[10]RUDKOWSKA I,Plant sterols and stanols for healthy ageing[J].Maturitas 2010,66(2):158-162.
[11]BOUIC P J.The role of phytosterols and phytosterolins in immune modulation:a review of the past 10 years[J].Curr Opin Clin Nutr Metab Care,2001,4(6):471-475.
[12]WOYENGO T A,RAMPRASATH V R,JONES P J H,Anticancer effects of phytosterols[J].European J Clin Nutr,2009,63(7):813-820.
[13]XIE X,XIAO N,HE J.Physiological function of phytosterol and its application[J].Animal Husbandry and Feed Science,2015(2):67-69.
[14]GUO M M,WANG Y Y,LUAN X,et al.Review of the extraction,physiological function and application in food of phytosterol[J].Journal of Food Safety&Quality,2014,7544:75443C-75443C-7.
[15]ZHANG Z X,CHANG C,LIU D B.Advance research on origin,function and extraction of natural phytosterol[J].Food&Machinery,2014.
[16]MOREIRA E A,BALTANáS M A.Recovery of phytosterols from sunflower oil deodorizer distillates[J].Journal of the American Oil Chemists Society,2004,81(2):161-167.
[17]庞利苹,徐雅琴.植物甾醇提纯及单体分离工艺的研究进展[J].中国粮油学报,2010,25(3):124-128.
[18]曹莹,谷克仁,孟冬,等.植物甾醇提取方法研究进展[J].粮油食品科技,2006,14(5):25-28.
[19]傅小峰.络合法提取植物甾醇的工艺研究及甾醇的溶解度测定[D].杭州:浙江大学,2004.
[20]谢慧明,张文成,王华锋,等.电磁诱导对葛根素结晶速率、晶体形貌及纯度影响研究[J].农业工程学报,2006,22(7):220-222.
[21]YANAGIYA S I,SAZAKI G,DURBIN S D,et al.Effects of a magnetic field on the growth rate of tetragonal lysozyme crystals[J].Journal of Crystal Growth,2000,208(1/4):645-650.
[22]龚晓明,葛红花,顾勇,等.磁处理对碳酸钙结晶以及沉降过程的影响[C].2010中国环境科学学会学术年会论文集(第三卷),2010.
[23]赵一凡,谷克仁,牛付欢.植物甾醇提取新工艺[J].食品科学,2008,29(7):192-196.
[24]吕双双,任婷婷,李书国.玉米胚芽油脱臭馏出物中植物甾醇和VE同时提取的研究[J].食品科技,2014(6):182-187.
[25]张海燕,张杰,尚富德.玉米须中高含量植物甾醇的提纯工艺[J].食品工业科技,2017,38(3):192-195.
[26]李俊杰.高γ-谷维素、β-谷甾醇米糠油的提取及精炼工艺研究[D].长沙:湖南农业大学,2016.
[27]万建春,姜波.结晶法分离纯化大豆甾醇中β-谷甾醇和豆甾醇单体[J].食品科技,2008,33(8):127-131.