牛粪好氧堆肥中真菌群落组成的动态特征
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摘要
采用高通量测序技术,探究牛粪堆肥过程中真菌群落时空动态变化、营养型及理化参数对真菌群落结构组成影响。结果表明,牛粪堆肥中未分类子囊菌门(Ascomycota)在升温期相对丰度最高,鬼伞属(Coprinus)和Guehomyces为高温期优势菌群,未分类子囊菌门为降温期优势菌属,未分类散囊菌目(Eurotiales)和Zopfiella为腐熟期优势菌群。在空间层次上,Guehomyces和未分类散囊菌目为上层和中层优势菌群,鬼伞属(Coprinus)和Zopfiella为下层优势菌群。在牛粪堆肥过程中腐生真菌相对丰度逐渐升高,堆肥下层相对丰度高于上、下两层。冗余分析(RDA)表明,含水率和温度对真菌群落结构变化影响显著(P<0.05)。牛粪堆肥过程中真菌群落结构组成在不同时期和空间上均存在差异,堆肥内真菌群落多数为腐生营养型,真菌群落结构变化与理化因子相关。
High-throughput sequencing was used to study the temporal and spatial dynamics of fungal community and its guilds during composting of cow manure, and the effects of physical and chemical parameters on the structure of fungal community. The results showed that the relative abundance of unclassified Ascomycota was the highest during heating stages, Coprinus and Guehomyces were the dominant fungi in high temperature stages, unclassified Ascomycota was the dominant fungi in cooling stages, unclassified Eurotiales and Zopfiella were the dominant fungi in maturity stages.At the spatial level, Guehomyces and unclassified Eurotiales were dominant fungi in the top and middle piles, while Coprinus and Zopfiella were dominant fungi in the botttom piles. The relative abundance of saprophytic fungi increased gradually during the composting of the cow manure, and the bottom pile was higher than that of the top and middle pile. Redundancy analysis showed that water content and temperature had significant effects on fungal community structure(P<0.05). During the composting process of cow manure, the composition of fungal community was different in temporal and spatial. Most fungal communities in the composting were saprophytic trophic. There was a correlation between the changes of fungal community structure and physicochemical factors.
High-throughput sequencing was used to study the temporal and spatial dynamics of fungal community and its guilds during composting of cow manure, and the effects of physical and chemical parameters on the structure of fungal community. The results showed that the relative abundance of unclassified Ascomycota was the highest during heating stages, Coprinus and Guehomyces were the dominant fungi in high temperature stages, unclassified Ascomycota was the dominant fungi in cooling stages, unclassified Eurotiales and Zopfiella were the dominant fungi in maturity stages.At the spatial level, Guehomyces and unclassified Eurotiales were dominant fungi in the top and middle piles, while Coprinus and Zopfiella were dominant fungi in the botttom piles. The relative abundance of saprophytic fungi increased gradually during the composting of the cow manure, and the bottom pile was higher than that of the top and middle pile. Redundancy analysis showed that water content and temperature had significant effects on fungal community structure(P<0.05). During the composting process of cow manure, the composition of fungal community was different in temporal and spatial. Most fungal communities in the composting were saprophytic trophic. There was a correlation between the changes of fungal community structure and physicochemical factors.
引文
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[25]朱丽平,许修宏,张文浩,等.牛粪堆肥中反硝化细菌与理化参数的关系[J].农业环境科学学报,2016,35(9):1781-1787.
[26]Garcia C,Ceccanti B,Masciandaro G,et al.Phosphatase and glucosidase activities in humic substances from animal wastes[J].Bioresource Technology,1995,53(1):79-87.
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[2]Juan A L,María C V.Biodiversity and succession of mycobiota associated to agricultural lignocellulosic waste-based composting[J].Bioresource Technology,2015,187:305-313.
[3]Zhang J,Zeng G M,Chen Y N,et al.Effects of physico-chemical parameters on the bacterial and fungal communities during agricultural waste composting[J].Bioresource Technology,2011,102:2950-2956.
[4]Meng Q X,Yang W,Men M Q,et al.Microbial community succession and response to environmental variables during cow manure and corn straw composting[J].Frontiers in Microbiology,2019,10:529.
[5]Ren G M,Xu X H,Qu J J,et al.Evaluation of microbial population dynamics in the co-composting of cow manure and rice straw using high throughput sequencing analysis[J].World Journal of Microbiology and Biotechnology,2016,32(6):101.
[6]许修宏,成利军,许本姝,等.基于高通量测序分析牛粪堆肥中细菌群落动态变化[J].东北农业大学学报,2018,49(3):10-20.
[7]鲍士旦.土壤农化分析[M].北京:中国农业出版社,2008.
[8]张文浩,门梦琪,许本姝,等.牛粪稻秸新型静态堆肥中真菌群落组成的动态特征[J].农业环境科学学报,2018,37(9):2029-2036.
[9]Bokulich N A,Mills D A.Improved selection of internal transcribed spacer-specific primers enables quantitative,ultra-highthroughput profiling of fungal communities[J].Applied and Environmental Microbiology,2013,79(8):2519-2526.
[10]Ihrmark K,B?deker I T M,Cruz-Martinez K,et al.New primers to amplify the fungal its2 region-evaluation by 454-sequencing of artificial and natural communities[J].Fems Microbiology Ecology,2012,82(3):666-677.
[11]栾冬梅,关静姝,孙黎,等.碳氮比对牛粪好氧堆肥过程的影响[J].东北农业大学学报,2008,39(8):77-81.
[12]Xiao Y,Zeng G M,Yang Z H,et al.Continuous thermophilic composting(CTC)for rapid biodegradation and maturation of organic municipal solid waste[J].Bioresource Technology,2009,100(20):4807-4813.
[13]刘佳,许修宏,李洪涛,等.利用PCR-DGGE技术对自然堆肥微生物群落多样性的分析[J].东北农业大学学报,2009,40(9):35-38.
[14]李思琦.应用PCR-DGGE分析添加菌剂对堆肥微生物群落多样性的影响[D].哈尔滨:东北农业大学,2015.
[15]徐丹妮.温度对蚯蚓处理城镇污泥过程微生物群落结构的影响[D].兰州:兰州交通大学,2017.
[16]Song C,Chi Z,Li J,et al.β-Galactosidase production by the psychrotolerant yeast Guehomyces pullulans 17-1 isolated from sea sediment in Antarctica and lactose hydrolysis[J].Bioprocess and Biosystems Engineering,2010,33(9):1025-1031.
[17]Klamer M,B??th E.Microbial community dynamics during composting of straw material studied using phospholipid fatty acid analysis[J].FEFEMS Microbiology Ecology,1998,27(1):9-20.
[18]Heinzkill M,Bech L,Halkier T,et al.Characterization of laccases and peroxidases from wood-rotting fungi(family Coprinaceae)[J].Applied and Environmental Microbiology,1998,64(5):1601-1606.
[19]Josefa B,Frutos C M,Jose A P,et al.Microbiota characterization of compost using omics approaches opens new perspectives for phytophthora root rot control[J].Plo S One,2016,11(8):e0158048.
[20]Wang K,Yin X B,Mao H L,et al.Changes in structure and function of fungal community in cow manure composting[J].Bioresource Technology,2018,255:123-130.
[21]聂三安,王祎,雷秀美,等.黄泥田土壤真菌群落结构和功能类群组成对施肥的响应[J].应用生态学报,2018,29(8):270-278.
[22]Seifert K,Morgan J G,Gams W,et al.The genera of hyphomycetes.CBS biodiversity series 9[M].Utrecht,The Netherlands:CBS-KNAW Fungal Biodiversity Centre,2011.
[23]张嘉超.基于分子生物学的堆肥功能微生物种群与体系基质特性关系研究[D].长沙:湖南大学,2013.
[24]唐聪.农业废物好氧堆肥中不同位置微生物群落分布特征的驱动机制响应研究[D].长沙:湖南大学,2013.
[25]朱丽平,许修宏,张文浩,等.牛粪堆肥中反硝化细菌与理化参数的关系[J].农业环境科学学报,2016,35(9):1781-1787.
[26]Garcia C,Ceccanti B,Masciandaro G,et al.Phosphatase and glucosidase activities in humic substances from animal wastes[J].Bioresource Technology,1995,53(1):79-87.
[27]Tiquia S M,Wan J H C,Tam N F Y.Dynamics of yard trimmings composting as determined by dehydrogenase activity,ATP content,arginineammonification,and nitrification potential[J].Process Biochemistry,2002,37(10):1057-1065.