摘要
为有效利用大鲵加工副产物大鲵皮,研究了大鲵皮胶原蛋白制备抗氧化肽的工艺条件。采用化学发光法,以羟自由基清除率为评价指标,通过单因素试验法,分别研究了酶的种类、酶添加量、酶解pH、温度和时间对大鲵皮胶原蛋白肽抗氧化活性的影响。在单因素试验的基础上,通过正交试验进一步优化了大鲵皮胶原蛋白制备抗氧化肽的工艺,确定了最适工艺条件:即酶添加量7000U/g,pH 8.5,温度55℃,时间4h,在此工艺条件下,大鲵皮胶原蛋白抗氧化肽清除羟自由基的清除率为83.15%。
In order to utilize the by-product of giant salamander during processing,the processing conditions for the preparation of antioxidant peptides from the giant salamander skin were studied. The hydroxyl radical scavenging rate was used as the evaluation index by chemiluminescence method. The single factor test method was used to study the effects of enzyme type,enzyme addition amount,enzymatic pH value,temperature and time on antioxidant activity of giant salamander collagen peptide. On the basis of single factor experiment,the process of preparing antioxidant peptides from the collagen skin was further optimized by orthogonal test,and the optimum technological conditions were determined:the enzyme addition amount was 7000 U/g,pH value was 8.5,temperature was 55℃ and time was 4 h,under these process conditions,the scavenging rate of scavenging hydroxyl radicals by giant salamander collagen antioxidant peptide was 83.15%.
引文
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