新疆阿魏内生真菌AW57菌液对小麦种子萌发及幼苗生长的影响
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摘要
【目的】分析新疆阿魏内生真菌AW57菌液对小麦种子萌发及幼苗生长的影响,研究有实用价值的内生菌株。【方法】以内生真菌AW57和新春31号小麦种子为材料,采用不同浓度的菌液浸种和浇灌处理方式,测定分析小麦种子的发芽率、发芽势以及幼苗的根长、根数、苗高、苗鲜重和苗干重等相关指标。【结果】浸种和喷施不同浓度AW57菌液均对小麦生长发育有促进作用,喷施20%浓度菌液小麦促生效果最佳,发芽率高达89.67%,苗鲜、干重分别比对照提高了54.41%、45.45%,对株高、叶长、叶宽和茎粗作用显著,对根数影响不显著,但对根长显著。【结论】适宜浓度AW57菌液有利于小麦生长,可作为小麦生长调节剂。
【Objective】 The effects of endophytic fungus AW57 from Xinjiang Ferula sinkiangensis K.M.Shen on the germination and seedling growth of wheat were studied, and endophytic strains with practical value were explored.【Method】The endophytic fungi AW57 and Xinchun No.31 wheat seeds were used as testing materials. The germination rate and germination potential of wheat seeds and the root length, root number, seedling height and seedling fresh weight were treated with different concentrations of bacterial liquid soaking and watering treatment. The relevant indicators such as fresh weight and dry weight of the seedlings were measured and analyzed. 【Result】Soaking seeds and spraying different concentrations of AW57 liquid all promoted the growth and development of wheat. The effect of spraying 20% concentration of bacteria liquid on wheat grow was the best effect, the germination rate was as high as 89.67%, and the seedling fresh and dry weight were 54.41% and 45.45% higher than those of the control, respectively. 54.41% and 45.45% had significant effects on plant height, leaf length, leaf width and stem diameter, but had no significant effect on root number, but significant on root length. The effect of spraying 20% concentration of bacteria solution on wheat growth was the best, the germination rate was as high as 89.67%, the seedling fresh and dry weight were 54.41% and 45.45% higher than those of the control, respectively. The effects on plant height, leaf length, leaf width and stem diameter were significant, but the effect on root number was not significant, but on root length was significant.【Conclusion】The suitable concentration of AW57 is beneficial to wheat growth and can be used as a wheat growth regulator.
【Objective】 The effects of endophytic fungus AW57 from Xinjiang Ferula sinkiangensis K.M.Shen on the germination and seedling growth of wheat were studied, and endophytic strains with practical value were explored.【Method】The endophytic fungi AW57 and Xinchun No.31 wheat seeds were used as testing materials. The germination rate and germination potential of wheat seeds and the root length, root number, seedling height and seedling fresh weight were treated with different concentrations of bacterial liquid soaking and watering treatment. The relevant indicators such as fresh weight and dry weight of the seedlings were measured and analyzed. 【Result】Soaking seeds and spraying different concentrations of AW57 liquid all promoted the growth and development of wheat. The effect of spraying 20% concentration of bacteria liquid on wheat grow was the best effect, the germination rate was as high as 89.67%, and the seedling fresh and dry weight were 54.41% and 45.45% higher than those of the control, respectively. 54.41% and 45.45% had significant effects on plant height, leaf length, leaf width and stem diameter, but had no significant effect on root number, but significant on root length. The effect of spraying 20% concentration of bacteria solution on wheat growth was the best, the germination rate was as high as 89.67%, the seedling fresh and dry weight were 54.41% and 45.45% higher than those of the control, respectively. The effects on plant height, leaf length, leaf width and stem diameter were significant, but the effect on root number was not significant, but on root length was significant.【Conclusion】The suitable concentration of AW57 is beneficial to wheat growth and can be used as a wheat growth regulator.
引文
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[8] Namdeo,A.,Patil,S.,& Fulzele,D.P.(2010).Influence of fungal elicitors on production of ajmalicine by cell cultures of catharanthus roseus.Biotechnology Progress,18(1),159-162.
[9] 郭顺星,徐锦堂.真菌在罗河石斛和铁皮石斛种子萌发中的作用[J].中国医学科学院学报,1991(1):46-49,81-84.GUO Shun-xing,XU Jin-tang.(1991).The role of fungi in the germination of Dendrobium nobile and Dendrobium candidum [J].Acta Academiae Medicinae Sinicae,(1):46-49,81-84.(in Chinese)
[10] 黄晖,邵士成,高江云.不同内生真菌对齿瓣石斛幼苗生长的效应[J].中国中药杂志,2016,41(11):2019-2024.HUANG Hui,SHAO Shi-cheng,GAO Jiang-yun,et al.(2016).Effects of different endophytic fungi on seedling growth of Dendrobium devonianum [J].China Journal of Chinese Materia Medica,41(11):2019-2024.(in Chinese)
[11] 徐欢,徐彩瑶,林勇明,等.5株内生真菌对杉木幼苗生长的影响[J].热带作物学报,2016,37(04):685-689.XU Huan,XU Cai-yao,LIN Yong-ming,et al.(2016).Effects of Five Endophytic Fungi on the Growth of Cunninghamia lanceolata Seedlings [J].Chinese Journal of Tropical Crops,37(4):685-689.(in Chinese)
[12] 宋文玲,戴传超,刘晓珍,等.不同内生真菌对菊花产量和品质的影响[J].中药材,2010,33(1):4-7.SONG Wen-ling,DAI Zhuang-chao,LIU Xiao-zhen,et al.(2010).Effects of Different Endophytic Fungi on Chrysanthemum Yield and Quality [J].Journal of Chinese Medicinal Materials,33(1):4-7.(in Chinese)
[13] 唐坤,李标,郭顺星.一株促进丹参生长和提高丹酚酸含量的活性内生真菌[J].菌物学报,2014,33(03):594-600.TANG Kun,LI Biao,GUO Shun-xing.(2014).An active endophytic fungus that promotes the growth of Salvia miltiorrhiza and increases salvianolic acid content [J].Mycosystema,33(3):594-600.(in Chinese)
[14] CARADUS J R.Genetic control of phosphorus uptake and pho-sphorus status in plants[J].Genetic manipulation of crop plantsto enhance integrated nutrient management in cropping systems,1995,15(1):55-74.
[15] Ju,L.T.,Han,Q.C.,& Jun,T.J.(2002).Studies on relationship between the character parameters of root and yield formation in rice.Scientia Agricultura Sinica,35(11),1416-1419.
[2] 谢彩香,石明辉,郭宝林,等.濒危野生新疆阿魏低空遥感资源调查[J].世界科学技术-中医药现代化,2014,16(11):2480-2486.XIE Cai-xia,SHI Ming-hui,GUO Bao-ling,et al.(2014).Investigation on low altitude remote Sensing Resources of Endangered Wild Xinjiang Ferula sinkiangensis K.M.Shen [J].Modernization of Traditional Chinese Medicine and Materia Materia-World Science and Technology,16(11):2480-2486.
[3] 中华人民共和国卫生部药典委员会.中国药典[S].1部.北京:化学工业出版社,2015:190.Pharmacopoeia Committee of the Ministry of Health of the people's Republic of China.(2015).Chinese pharmacopoeia [M].Beijing:Chemical Industry Publishing House:190.(in Chinese)
[4] Pertrini,O.(1991).Fungal endophytes of tree leaves[M].//Andrews J H,Hirano S S.Microbial ecology of leaves.New York:Spri-ger-verlag,1991:179-197.
[5] 陈贝贝,王敏,胡鸢雷,等.地黄内生真菌促生作用的初步研究[J].中国中药杂志,2011,36(09):1137-1140.查不到?CHEN Bei-bei,WANG Ming,HU Yuan-lei,et al.(2011).Preliminary study on the growth promoting effect of endophytic fungi from Rehmannia glutinosa [J].China Journal of Chinese Materia Medica,36(9):1137-1140.(in Chinese)
[6] 詹寿发,卢丹妮,毛花英,等.2株溶磷、解钾与产IAA的内生真菌菌株的筛选、鉴定及促生作用研究[J].中国土壤与肥料,2017,269(3):142-151.ZHAN Shou-fa,LU Dan-ni,MAO Hua-ying,et al.(2017).Screening,Identification and Promoting Effect of Two Strains of Endophytic Fungi Dissolved in Phosphorus,Potassium and IAA [J].Soil and Fertilizer Sciences in China,69(3):142-151.(in Chinese)
[7] Júlia Kuklinsky-Sobral,Welington Luiz Araújo,Mendes,R.,Isaias Olívio Geraldi,Pizzirani-Kleiner,A.A.,& Lúcio Azevedo.(2004).Isolation and characterization of soybean-associated bacteria and their potential for plant growth promotion.Environmental microbiology,6(12),1244-1251.
[8] Namdeo,A.,Patil,S.,& Fulzele,D.P.(2010).Influence of fungal elicitors on production of ajmalicine by cell cultures of catharanthus roseus.Biotechnology Progress,18(1),159-162.
[9] 郭顺星,徐锦堂.真菌在罗河石斛和铁皮石斛种子萌发中的作用[J].中国医学科学院学报,1991(1):46-49,81-84.GUO Shun-xing,XU Jin-tang.(1991).The role of fungi in the germination of Dendrobium nobile and Dendrobium candidum [J].Acta Academiae Medicinae Sinicae,(1):46-49,81-84.(in Chinese)
[10] 黄晖,邵士成,高江云.不同内生真菌对齿瓣石斛幼苗生长的效应[J].中国中药杂志,2016,41(11):2019-2024.HUANG Hui,SHAO Shi-cheng,GAO Jiang-yun,et al.(2016).Effects of different endophytic fungi on seedling growth of Dendrobium devonianum [J].China Journal of Chinese Materia Medica,41(11):2019-2024.(in Chinese)
[11] 徐欢,徐彩瑶,林勇明,等.5株内生真菌对杉木幼苗生长的影响[J].热带作物学报,2016,37(04):685-689.XU Huan,XU Cai-yao,LIN Yong-ming,et al.(2016).Effects of Five Endophytic Fungi on the Growth of Cunninghamia lanceolata Seedlings [J].Chinese Journal of Tropical Crops,37(4):685-689.(in Chinese)
[12] 宋文玲,戴传超,刘晓珍,等.不同内生真菌对菊花产量和品质的影响[J].中药材,2010,33(1):4-7.SONG Wen-ling,DAI Zhuang-chao,LIU Xiao-zhen,et al.(2010).Effects of Different Endophytic Fungi on Chrysanthemum Yield and Quality [J].Journal of Chinese Medicinal Materials,33(1):4-7.(in Chinese)
[13] 唐坤,李标,郭顺星.一株促进丹参生长和提高丹酚酸含量的活性内生真菌[J].菌物学报,2014,33(03):594-600.TANG Kun,LI Biao,GUO Shun-xing.(2014).An active endophytic fungus that promotes the growth of Salvia miltiorrhiza and increases salvianolic acid content [J].Mycosystema,33(3):594-600.(in Chinese)
[14] CARADUS J R.Genetic control of phosphorus uptake and pho-sphorus status in plants[J].Genetic manipulation of crop plantsto enhance integrated nutrient management in cropping systems,1995,15(1):55-74.
[15] Ju,L.T.,Han,Q.C.,& Jun,T.J.(2002).Studies on relationship between the character parameters of root and yield formation in rice.Scientia Agricultura Sinica,35(11),1416-1419.