γ-聚谷氨酸体外抗氧化性及抑制透明质酸酶活性的研究
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摘要
目的考察γ-聚谷氨酸体外抗氧化活性及对透明质酸酶的抑制活性。方法利用DPPH法测定高相对分子质量(Mr)(140万)和低Mr(10万)γ-聚谷氨酸的DPPH自由基清除率曲线,以IC_(50)值作为评价试样清除DPPH自由基能力的指标,并与抗氧化剂Vc磷酸酯镁和α-熊果苷比较,评价两种Mrγ-聚谷氨酸的抗氧化性;同时利用DNS法测定以上两种M_r的γ-聚谷氨酸对透明质酸酶的抑制活性。结果 140万Mrγ-聚谷氨酸清除DPPH自由基的IC_(50)值为25.5 mg/mL,略优于10万Mrγ-聚谷氨酸(IC_(50) 30.5 mg/mL)。两者清除自由基的效果约为Vc磷酸酯镁(IC_(50) 13 mg/mL)的一半,低于α-熊果苷(IC_(50) 0.016 mg/mL);另外,两种M_rγ-聚谷氨酸在低浓度(0.008%)条件下即可抑制约50%透明质酸酶活性,且其抑制率呈浓度依赖性。结论γ-聚谷氨酸是一种优良的化妆品原料,除具有超强的保湿作用之外,还具有中等程度的抗氧化活性及良好的透明质酸酶抑制活性,表明其在功能性化妆品中具有广阔的应用前景。
Objective To investigate the anti-oxidant activity of γ-polyglutamic acid(γ-PGA) and its inhibitory activity against hyaluronidase in vitro.Methods Taking IC_(50) as the evaluating indicator of DPPH free radical scavenging capability,the scavenging rate curves of γ-polyglutamic acid with high and low Mr were determined.Vc magnesium phosphate and α-arbutin were used as control.The inhibitory activity of γ-polyglutamic acid with high and low Mragainst hyaluronidase was determined by DNS method.Results The IC_(50) value of γ-polyglutamic acid with Mr of 1.4×10~6 was 25.5 mg/mL,slightly better than that of γ-polyglutamic acid with Mr of 100×10~3(IC_(50) 30.5 mg/mL).The abilities to scavenge free radicals were about half of that of Vc magnesium phosphate(IC_(50) 13 mg/mL),and were lower than that of α-arbutin(IC_(50) 0.016 mg/mL).In addition,two kinds of γ-polyglutamic acid could inhibit about 50 % activity of hyaluronidase under low concentration(0.008 %),and the inhibition rate was concentration dependent.Conclusionγ-polyglutamic acid is an excellent cosmetic raw material with a general degree of anti-oxidant activity and favourable inhibitory activity against hyaluronidase besides its strong moisturizing effect,indicating a broad application aspect in the functional cosmetics.
Objective To investigate the anti-oxidant activity of γ-polyglutamic acid(γ-PGA) and its inhibitory activity against hyaluronidase in vitro.Methods Taking IC_(50) as the evaluating indicator of DPPH free radical scavenging capability,the scavenging rate curves of γ-polyglutamic acid with high and low Mr were determined.Vc magnesium phosphate and α-arbutin were used as control.The inhibitory activity of γ-polyglutamic acid with high and low Mragainst hyaluronidase was determined by DNS method.Results The IC_(50) value of γ-polyglutamic acid with Mr of 1.4×10~6 was 25.5 mg/mL,slightly better than that of γ-polyglutamic acid with Mr of 100×10~3(IC_(50) 30.5 mg/mL).The abilities to scavenge free radicals were about half of that of Vc magnesium phosphate(IC_(50) 13 mg/mL),and were lower than that of α-arbutin(IC_(50) 0.016 mg/mL).In addition,two kinds of γ-polyglutamic acid could inhibit about 50 % activity of hyaluronidase under low concentration(0.008 %),and the inhibition rate was concentration dependent.Conclusionγ-polyglutamic acid is an excellent cosmetic raw material with a general degree of anti-oxidant activity and favourable inhibitory activity against hyaluronidase besides its strong moisturizing effect,indicating a broad application aspect in the functional cosmetics.
引文
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[2]王卫国,王卫,赵永亮,等.γ-聚谷氨酸的研究及应用进展[J].河南工业大学学报,2016,37(2):117-122.
[3]王浩,杨丽萍,乔君,等.γ-聚谷氨酸的研究进展[J].山东食品发酵,2011,4:30-34.
[4]刘霞,刘飞,刘少英,等.聚谷氨酸的保湿功效及安全性评价[J].日用化学工业,2015,45(5):275-278.
[5]郑雨.化妆品原料抗氧化剂[J].日用化学品科学,2010,33(12):52-54.
[6]李春阳,许时婴,王璋.DPPH法测定葡萄籽原花青素清除自由基的能力[J].食品与生物技术学报,2006,25(2):102-106.
[7]刘帅涛,陶慧林,李锦艳.4种黄酮小分子对DPPH自由基的清除作用及构效关系研究[J].分析测试学报,2012,31(1):71-75.
[8]张志国,陈锦屏,邵秀芝,等.红枣核类黄酮清除DPPH自由基活性研究[J].食品科学,2007,28(2):67-70.
[9]杨素珍,阚洪玲,张天民.透明质酸在美容化妆品方面的应用[J].食品与药品,2010,12(7):275-277.
[10]陈祥娥,凌沛学.透明质酸与化妆品[J].食品与药品,2010,12(7):278-280.
[11]王晓玉.重组人透明质酸酶PH-20在毕赤酵母中的表达,发酵条件优化及性质研究[D].山东:山东大学,2011.