摘要
目的考察γ-聚谷氨酸体外抗氧化活性及对透明质酸酶的抑制活性。方法利用DPPH法测定高相对分子质量(Mr)(140万)和低Mr(10万)γ-聚谷氨酸的DPPH自由基清除率曲线,以IC_(50)值作为评价试样清除DPPH自由基能力的指标,并与抗氧化剂Vc磷酸酯镁和α-熊果苷比较,评价两种Mrγ-聚谷氨酸的抗氧化性;同时利用DNS法测定以上两种M_r的γ-聚谷氨酸对透明质酸酶的抑制活性。结果 140万Mrγ-聚谷氨酸清除DPPH自由基的IC_(50)值为25.5 mg/mL,略优于10万Mrγ-聚谷氨酸(IC_(50) 30.5 mg/mL)。两者清除自由基的效果约为Vc磷酸酯镁(IC_(50) 13 mg/mL)的一半,低于α-熊果苷(IC_(50) 0.016 mg/mL);另外,两种M_rγ-聚谷氨酸在低浓度(0.008%)条件下即可抑制约50%透明质酸酶活性,且其抑制率呈浓度依赖性。结论γ-聚谷氨酸是一种优良的化妆品原料,除具有超强的保湿作用之外,还具有中等程度的抗氧化活性及良好的透明质酸酶抑制活性,表明其在功能性化妆品中具有广阔的应用前景。
Objective To investigate the anti-oxidant activity of γ-polyglutamic acid(γ-PGA) and its inhibitory activity against hyaluronidase in vitro.Methods Taking IC_(50) as the evaluating indicator of DPPH free radical scavenging capability,the scavenging rate curves of γ-polyglutamic acid with high and low Mr were determined.Vc magnesium phosphate and α-arbutin were used as control.The inhibitory activity of γ-polyglutamic acid with high and low Mragainst hyaluronidase was determined by DNS method.Results The IC_(50) value of γ-polyglutamic acid with Mr of 1.4×10~6 was 25.5 mg/mL,slightly better than that of γ-polyglutamic acid with Mr of 100×10~3(IC_(50) 30.5 mg/mL).The abilities to scavenge free radicals were about half of that of Vc magnesium phosphate(IC_(50) 13 mg/mL),and were lower than that of α-arbutin(IC_(50) 0.016 mg/mL).In addition,two kinds of γ-polyglutamic acid could inhibit about 50 % activity of hyaluronidase under low concentration(0.008 %),and the inhibition rate was concentration dependent.Conclusionγ-polyglutamic acid is an excellent cosmetic raw material with a general degree of anti-oxidant activity and favourable inhibitory activity against hyaluronidase besides its strong moisturizing effect,indicating a broad application aspect in the functional cosmetics.
引文
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