三穗鸭ODC1基因多态性与屠体性状关联性分析
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摘要
[目的]寻找与三穗鸭屠体性状相关的遗传标记。[方法]以139只贵州三穗鸭为研究对象,采用PCR-SSCP技术和DNA测序方法,对鸟氨酸脱羧酶1(Ornithine decarboxylase,ODC1)基因进行遗传多态性研究。[结果]在ODC1基因中检测到1个碱基变异,位于第4外显子109 bp处,为A→G突变,且在此位点的优势等位基因型为AA基因型,A为优势等位基因。ODC1基因Ex4处多态性对三穗鸭的腿肌重、半净膛重等屠体性状存在显著影响(P<0.05)。[结论]研究初步推测ODC1基因多态性与屠体性状具有显著的影响,A109G可作为穗鸭屠体性状的遗传标记,为建立三穗鸭的分子辅助标记选择方法提供初步依据,推进选育进程。
[Objective]To look for the molecular genetic makers in relation to carcass traits of Sansui laying duck. [Methods]The candidate genes of Ornithine decarboxylase gene( ODC1) were conducted to polymorphic analysis and association study with carcass traits in population of 139 Sansui ducks in Guizhou province based on PCR- SSCP. [Results]A→G mutation in109 bp of ODC1 gene exon 4 was detected,Allele A was identified as the dominant gene and AA was dominant genotype. Ex4 of ODC1 gene exist significant effect on semi- eviscerated weight and leg muscle weight( P < 0. 05). [Conclusion]The results revealed that SNPs of ODC1 gene had effect on carcass traits in Sansui duck,A109 G site could be used as effective genetic markers for molecular breeding and improved the breeding process.
[Objective]To look for the molecular genetic makers in relation to carcass traits of Sansui laying duck. [Methods]The candidate genes of Ornithine decarboxylase gene( ODC1) were conducted to polymorphic analysis and association study with carcass traits in population of 139 Sansui ducks in Guizhou province based on PCR- SSCP. [Results]A→G mutation in109 bp of ODC1 gene exon 4 was detected,Allele A was identified as the dominant gene and AA was dominant genotype. Ex4 of ODC1 gene exist significant effect on semi- eviscerated weight and leg muscle weight( P < 0. 05). [Conclusion]The results revealed that SNPs of ODC1 gene had effect on carcass traits in Sansui duck,A109 G site could be used as effective genetic markers for molecular breeding and improved the breeding process.
引文
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[11]俞菊华,李红霞,唐永凯,等.鲤鱼鸟氨酸脱羧酶基因的序列特征和表达[J].水生生物学报,2013,37(3):566-571.
[12]俞明月.产蛋性状相关基因标记的研究[D].南京:南京师范大学,2006.
[13]Parsanejad R,Praslickova D,Zadworny D,et al.Ornithine decarboxylase:Haplotype structure and trait associations in white leghorn chickens[J].Poult Sci,2004,83(9):1518-1523.
[14]Y.Uemoto,S.Sato and T.Ohtake.Ornithine decarboxylase gene is a positional candidate gene affecting growth and carcass traits in F2 intercross chickens[J].Poultry Science,2011,90(1):35-41.
[2]徐霞.鸟氨酸脱羧酶(ODC)及转录因子RUNX3在胃癌中表达失调的分子机制研究[D].济南:山东大学,2012.
[3]李友忠,葛圣雷,杨新明,等.鸟氨酸脱羧酶和谷氨酸脱羧酶65在鼻腔鼻窦良恶性病变中的表达及意义[J].肿瘤防治研究,2009,36(12):1020-1023.
[4]Jason A.Zell,Argyrios Ziogas,Natalia Ignatenko,et al.Associations of a polymorphism in the ornithine decarboxylase gene with colorectal cancer survival[J].Cancer,2009,15(19):6208-6216.
[5]Lisa Y.Cho.Jae Jeong Yang.Kwang-Pil Ko,et al.Gene polymorphisms in the ornithine decarboxylase–polyamine pathway modify gastric cancer risk by interaction with isoflavone concentrations[J].Gastric Cancer,2015,18(3):495-503.
[6]Andrew J.Symes,Marte Eilertsen,Michael Millar,et al.Quantitative analysis of BTF3,HINT1,NDRG1 and ODC1 protein overexpression in human prostate cancer tissue[J].PLo S ONE,2013,8(12):1-9.
[7]Raut CP,lzzo F,Marra P.et a1.Significant long-term survival after radiofrequency ablation of unresectable hepatocellular carcinoma in patients with cirrhosis[J].Ann Surg Oncol,2005,12(8):6l6-628.
[8]Uemoto Y,Sato S,Ohtake T,et al.Ornithine decarboxylase gene is a positional candidate gene affecting growth and carcass traits in F2intercross chickens[J].Poultry Science,2011,90(1):35-41.
[9]胡海燕,刘贤锡,姜春英,等.人ODC重组蛋白及其多抗的制备和临床应用[J].中国现代普通外科,2004,7(1):26-30.
[10]李红霞,李建林,唐永凯,等.建鲤ODC1基因型与增重的相关性分析[J].水生生物学报,2014,38(3):414-421.
[11]俞菊华,李红霞,唐永凯,等.鲤鱼鸟氨酸脱羧酶基因的序列特征和表达[J].水生生物学报,2013,37(3):566-571.
[12]俞明月.产蛋性状相关基因标记的研究[D].南京:南京师范大学,2006.
[13]Parsanejad R,Praslickova D,Zadworny D,et al.Ornithine decarboxylase:Haplotype structure and trait associations in white leghorn chickens[J].Poult Sci,2004,83(9):1518-1523.
[14]Y.Uemoto,S.Sato and T.Ohtake.Ornithine decarboxylase gene is a positional candidate gene affecting growth and carcass traits in F2 intercross chickens[J].Poultry Science,2011,90(1):35-41.