外源SA对李花抗坏血酸-谷胱甘肽循环的影响
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摘要
为研究外源水杨酸(SA)对李花抗坏血酸-谷胱甘肽循环以及对李花抗寒性的影响,以‘大石早生李’‘安哥诺李’2个品种为试材,以喷水处理为对照,研究了喷施适宜浓度的外源水杨酸(SA)对-2℃低温处理4h后2个品种的李花H_2O_2质量摩尔浓度以及对抗坏血酸-谷胱甘肽循环中酶及抗氧化物质的影响。结果表明:0.20、0.15 mmol·L~(-1)SA可以分别降低‘大石早生李’‘安哥诺李’的H_2O_2质量摩尔浓度,显著增强谷胱甘肽过氧化物酶(GPX)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)、脱氢抗坏血酸还原酶(DHAR)活性,提高抗坏血酸(AsA)、谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)、总抗坏血酸、总谷胱甘肽质量摩尔浓度和AsA/DHA、GSH/GSSG。低温胁迫下,AsA-GSH循环受到破坏,但喷施适宜浓度的外源SA可以显著增强李花的抗氧化酶活性,提高抗氧化物质质量摩尔浓度,降低李花的膜质过氧化作用,降低低温对细胞膜的破坏,维持AsA-GSH循环系统的稳定性,从而一定程度上提高李花的抗寒性。
To investigate the effects of exogenous SA hormones on AsA-GSH cycle and the cold resistance of plum flower,with water as control,we studied the effects of exogenous SA in the proper concentration on the peroxide content and ascorbate-glutathione cycle of Prunus salicina‘Oishiwas’and‘Angeleno’flowers under-2℃chilling stress.The 0.2 mmol·L~(-1) SA for P.salicina‘Oishiwas’,0.15 mmol·L~(-1)SA for‘Angeleno’could reduce the H_2O_2 content and significantly enhance the activities of APX,DHAR,GR and GPX.The contents of AsA,GSH,GSSG,AsA+DHA,GSH+GSSG and the rations of AsA/DHA,GSH/GSSG were higher than those of the control.Therefore,low temperature stress could block AsA-GSH cycle.Compared with the control,the application of suitable content of exogenous SA could significantly enhance activities of antioxidant enzymes,enhance contents of antioxidants,reduce the membrane lipid peroxidation as well as the destruction of cell membrane,and maintain the stability of AsA-GSH cycle system to improve the cold resistance of plum flowers.
To investigate the effects of exogenous SA hormones on AsA-GSH cycle and the cold resistance of plum flower,with water as control,we studied the effects of exogenous SA in the proper concentration on the peroxide content and ascorbate-glutathione cycle of Prunus salicina‘Oishiwas’and‘Angeleno’flowers under-2℃chilling stress.The 0.2 mmol·L~(-1) SA for P.salicina‘Oishiwas’,0.15 mmol·L~(-1)SA for‘Angeleno’could reduce the H_2O_2 content and significantly enhance the activities of APX,DHAR,GR and GPX.The contents of AsA,GSH,GSSG,AsA+DHA,GSH+GSSG and the rations of AsA/DHA,GSH/GSSG were higher than those of the control.Therefore,low temperature stress could block AsA-GSH cycle.Compared with the control,the application of suitable content of exogenous SA could significantly enhance activities of antioxidant enzymes,enhance contents of antioxidants,reduce the membrane lipid peroxidation as well as the destruction of cell membrane,and maintain the stability of AsA-GSH cycle system to improve the cold resistance of plum flowers.
引文
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[21]李秀,巩彪,徐坤.外源NO对高温胁迫下姜叶片活性氧代谢的影响[J].园艺学报,2014,41(2):277-284.
[22]LIN Y,LIU Z,SHI Q,et al.Exogenous nitric oxide(NO)increased antioxidant capacity of cucumber hypocotyl and radicle under salt stress[J].Scientia Horticulturae,2012,142(4):118-127.
[23]SHALATA A,MITTOVA V,VOLOKITA M,et al.Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative stress:The root antioxidative system[J].Physiologia Plantarum,2001,112(4):487-494.
[2]宋俊伟.三种长效基肥对李果园土壤养分及果实产量品质的影响[D].保定:河北农业大学,2015.
[3]HEINO P,SANDMAN G,Lang V,et al.Abscisic acid deficiency prevents development of freezing tolerance in Arabidopsis thaliana(L.)Heynh[J].Theoretical and Applied Genetics,1990,79(6):801-806.
[4]张俊环,王玉柱,孙浩元,等.外源水杨酸对低温下杏花抗氧化酶和CBF转录因子表达的影响[J].植物生理学报,2014.50(2):171-177.
[5]NOCTOR G,FOYER C H.Ascorbate and glutathione:keeping active oxygen under control[J].Annual Review of Plant Physiology&Plant Molecular Biology,1998,49:249-279.
[6]孟庆瑞.杏花器官霜冻害生理机制研究[D].保定:河北农业大学,2009.
[7]林植芳,李双顺,林桂珠,等.衰老叶片和叶绿体中H2O2的累积与膜脂过氧化的关系[J].植物生理学报,1988,14(1):12-16.
[8]沈文飚,徐朗莱,叶茂炳,等.抗坏血酸过氧化物酶活性测定的探讨[J].植物生理学通讯,1996,32(3):203-205.
[9]MA F,CHENG L.The sun-exposed peel of apple fruit has higher xanthophyll cycle-dependent thermal dissipation and antioxidants of the ascorbate-glutathione pathway than the shaded peel[J].Plant Science,2003,165(4):819-827.
[10]张景萍,吴珍龄.几种植物中谷胱甘肽过氧化物酶活性测定[J].广西农业科学,2004,35(3):177-178.
[11]CAKMAK I,MARSCHNER H.Magnesium deficiency and high light intensity enhance activities of superoxide dismutase,ascorbate peroxidase,and glutathione reductase in bean leaves[J].Plant Physiology,1992,98(4):1222-1227.
[12]ELLMAN G L.Tissue sulfhydryl groups[J].Archives of Biochemistry&Biophysics,1959,82(1):70.
[13]MEYER A J.The integration of glutathione homeostasis and redox signaling[J].Journal of Plant Physiology,2008,165(13):1390-1403.
[14]SEN C K,PACKER L.Antioxidant and redox regulation of gene transcription[M].Pittsburgh:Academic Press,1996.
[15]孟庆瑞,王文凤,梁隐泉,等.杏品种花器官过冷却点及结冰点的研究[J].中国农业科学,2008,41(4):1128-1133.
[16]宁超,孟庆瑞,李淑贤,等.抗霜冻仁用杏优株花器官抗寒性的比较研究[J].河北农业大学学报,2010,33(3):37-41.
[17]郑小林,陈燕,敬国兴,等.草酸处理对札果采后果实As AGSH循环系统的影响[J].园艺学报,2011,38(9):1633-1640.
[18]JIN Y H,TAO D L,HAO Z Q,et al.Environmental stresses and redox status of ascorbate[J].Acta Botanica Sinica,2003,45(7):795-801.
[19]王聪,朱月林,杨立飞,等.Na Cl胁迫对菜用大豆种子抗坏血酸-谷胱甘肽循环的影响[J].植物营养与肥料学报,2010,16(5):1209-1216.
[20]FOYER C H,NOCTOR G.Ascorbate and glutathione:the heart of the redox hub[J].Plant Physiology,2011,155:2-18.
[21]李秀,巩彪,徐坤.外源NO对高温胁迫下姜叶片活性氧代谢的影响[J].园艺学报,2014,41(2):277-284.
[22]LIN Y,LIU Z,SHI Q,et al.Exogenous nitric oxide(NO)increased antioxidant capacity of cucumber hypocotyl and radicle under salt stress[J].Scientia Horticulturae,2012,142(4):118-127.
[23]SHALATA A,MITTOVA V,VOLOKITA M,et al.Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative stress:The root antioxidative system[J].Physiologia Plantarum,2001,112(4):487-494.