外源褪黑素对高温胁迫下茄子幼苗光合和生理特性的影响
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摘要
以茄子(Solanum melongena)为试材,利用人工气候室进行高温处理,研究外源褪黑素(MT)对高温胁迫下茄子幼苗叶绿素含量、气体交换参数、碳水化合物含量以及相关酶活性的影响。结果表明,与对照相比,高温处理下茄子幼苗叶片叶绿素含量、核酮糖-1,5-二磷酸羧化酶/加氧酶(RuBisCO)活性和净光合速率(P_n)均显著降低,而气孔导度(G_s)、胞间CO_2浓度(C_i)、蒸腾速率(T_r)均显著增加;可溶性糖、蔗糖、果糖含量和蔗糖合成酶(SS)活性显著升高,淀粉含量以及酸性转化酶(AI)、中性转化酶(NI)和蔗糖磷酸合成酶(SPS)活性均显著降低。与单纯高温胁迫相比,叶片喷施外源MT除使C_i降低外,碳水化合物含量及代谢相关酶活性均显著提高,缓解了高温胁迫对茄子幼苗光合色素合成及光合作用的抑制。同时,外源MT能显著降低高温胁迫下茄子幼苗叶片丙二醛(MDA)含量、超氧阴离子(O_2ˉ·)产生速率和过氧化氢(H_2O_2)含量,增强茄子幼苗叶片中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)活性,提高抗坏血酸(AsA)、谷胱甘肽(GSH)、脯氨酸(Pro)、可溶性蛋白质含量。以上结果说明外源MT可通过提高光合色素含量增强茄子植株碳水化合物代谢的能力,调节代谢相关酶活性,抑制高温胁迫条件下茄子幼苗体内活性氧(ROS)的产生,提高抗氧化酶系的活性及抗氧化物质的含量,降低膜质过氧化水平,保护脂膜的完整性,从而提高幼苗抵抗高温胁迫的能力。
This paper studies the effect of exogenous melatonin(MT)on chlorophyll contents,photosynthetic properties and carbohydrate metabolism of eggplant(Solanum melongena)under high temperature treatment in an artificial climate chamber.The results show that compared to the control seedlings,chlorophyll contents,ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBisCO)activity and net photosynthetic rate(P_n)under high temperature treatment were significantly decreased.Stomatal conductance(G_s),intercellular CO_2 concentration(C_i)and transpiration rate(T_r)were significantly increased.The contents of soluble sugar,sucrose and fructose and sucrose synthase(SS)activity were significantly enhanced.Starch content and activities of acid invertase(AI),neutral invertase(NI)and sucrose phosphate synthase(SPS)were significantly decreased.Exogenous MT treatment significantly improved the photosynthesis,carbohydrate contents and activities of the four enzymes in the seedling leaves except decreased C_i compared to the high temperature treatment.The exogenous MT could sinificantly decrease malonaldehyde(MDA)content,superoxide anion producing rate(O_2ˉ·)and hydrogen peroxide(H_2O_2)content,but increase activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and ascorbate peroxidase(APX)as well as contents of ascorbic acid(AsA),glutathione(GSH),proline and soluble protein.The above results show that MT could improve the ability of eggplant seedlings through enhancing chlorophyll contents,regulating carbohydrate metabolism,and promoting the formation of new metabolic balance of eggplant seedlings under high temperature stress,and the results illustrate that pretreatment with MT could inhibit reactive oxygen species(ROS),raise antioxidative enzyme activities and antioxidant contents,and decrease membrane lipid peroxidation to alleviate the damage of high temperature stress to the seedlings.
This paper studies the effect of exogenous melatonin(MT)on chlorophyll contents,photosynthetic properties and carbohydrate metabolism of eggplant(Solanum melongena)under high temperature treatment in an artificial climate chamber.The results show that compared to the control seedlings,chlorophyll contents,ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBisCO)activity and net photosynthetic rate(P_n)under high temperature treatment were significantly decreased.Stomatal conductance(G_s),intercellular CO_2 concentration(C_i)and transpiration rate(T_r)were significantly increased.The contents of soluble sugar,sucrose and fructose and sucrose synthase(SS)activity were significantly enhanced.Starch content and activities of acid invertase(AI),neutral invertase(NI)and sucrose phosphate synthase(SPS)were significantly decreased.Exogenous MT treatment significantly improved the photosynthesis,carbohydrate contents and activities of the four enzymes in the seedling leaves except decreased C_i compared to the high temperature treatment.The exogenous MT could sinificantly decrease malonaldehyde(MDA)content,superoxide anion producing rate(O_2ˉ·)and hydrogen peroxide(H_2O_2)content,but increase activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and ascorbate peroxidase(APX)as well as contents of ascorbic acid(AsA),glutathione(GSH),proline and soluble protein.The above results show that MT could improve the ability of eggplant seedlings through enhancing chlorophyll contents,regulating carbohydrate metabolism,and promoting the formation of new metabolic balance of eggplant seedlings under high temperature stress,and the results illustrate that pretreatment with MT could inhibit reactive oxygen species(ROS),raise antioxidative enzyme activities and antioxidant contents,and decrease membrane lipid peroxidation to alleviate the damage of high temperature stress to the seedlings.
引文
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Hu Z,Fan J,Xie Y,et al(2016).Comparative photosynthetic and metabolic analyses reveal mechanism of improved cold stress tolerance in bermudagrass by exogenous melatonin.Plant Physiol Bioch,100:94-104
Jiang C,Zu C(2015).Advances in melatonin and its roles in abiotic stress resistance in plants.Biotechnol Bull,31(4):47-55(in Chinese with English abstract)[姜超强,祖朝龙(2015).褪黑素与植物抗逆性研究进展.生物技术通报,31(4):47-55]
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Liu J,Wang W,Wang L,et al(2015).Exogenous melatonin improves seedling health index and drought tolerance in tomato.Plant Growth Regul,77:317-326
Lorenzen JH,Lafra AM.Effect of heat stress on enzymes that affect sucrose levels in potato shoots.J Am Soc Hortic Sci,121:1152-1156
Osório ML,Osório J,Romano A(2013).Photosynthesis,energy partitioning,and metabolic adjustments of the endangered Cistaceae species Tuberaria major under high temperature and drought.Photosynthetica,51(1):75-84
Sang QQ,Shu S,Shan X,et al(2016).Effects of exogenous spermidine on antioxidant system of tomato seedlings exposed to high temperature stress.Russ J Plant Physiol,63(5):645-655
Shi H,Tan DX,Reiter RJ,et al(2015).Melatonin induces class A1 heat-shock factors(HSFA1s)and their possible involvement of thermotolerance in Arabidopsis.J Pineal Res,58(3):335-342
Suleman P,Redha A,Afzal M,et al(2012).Temperature-induced changes of malondialdehyde,heat-shock proteins in relation to chlorophyll fluorescence and photosynthesis in Conocarpus lancifolius(Engl.).Acta Physiol Plant,35:1223-1231
Sun SN,Wang Q,Sun CC,et al(2017).Response and adaptation of photosynthesis of cucumber seedlings to high temperature stress.Chin J Appl Ecol,28(5):1603-1610(in Chinesewith English abstract)[孙胜楠,王强,孙晨晨等(2017).黄瓜幼苗光合作用对高温胁迫的响应与适应.应用生态学报,28(5):1603-1610]
Sun Y,Guo S,Xu G(2015).Effect of root-zone hypoxia stress on carbohydrate contents of muskmelon seedlings.Acta Bot Boreal-Occident Sin,35(1):168-174(in Chinese with English abstract)[孙艳军,郭世荣,徐刚(2015).根际低氧胁迫对网纹甜瓜幼苗植株碳水化合物含量的影响.西北植物学报,35(1):168-174]
Tan DX,Manchester LC,Helton P,et al(2007).Phytoremediative capacity of plants enriched with melatonin.Plant Signal Behav,2:514-516
Wang R,Yang XL,Xu H,et al(2016).Research progress of melatonin biosynthesis and metabolism in higher plants.Plant Physiol J,52(5):615-627(in Chinese with English abstract)[王蕊,杨小龙,须晖等(2016).高等植物褪黑素的合成和代谢研究进展.植物生理学报,52(5):615-627]
Wu X,Zhu ZW,Zhang AD,et al(2017).Effects of exogenous melatonin on the growth,photosynthesis and antioxidant system in eggplant(Solanum melongena L.)seedlings under low temperature stress.Acta Bot Boreal-Occident Sin,37(12):2427-2434(in Chinese with English abstract)[吴雪霞,朱宗文,张爱冬等(2017).外源褪黑素对低温胁迫下茄子幼苗生长及其光合作用和抗氧化系统的影响.西北植物学报,37(12):2427-2434]
Wu XX,Zhu YL,Zhu WM,et al(2006).Physiological effects of exogenous nitric oxide in tomato seedlings under NaCl stress.Sci Agr Sin,39(3):575-581(in Chinese with English abstract)[吴雪霞,朱月林,朱为民等(2006).外源一氧化氮对NaCl胁迫下番茄幼苗生理影响.中国农业科学,39(3):575-581]
Xia Y(2013).The effects of elevated CO2 with high temperature stress on the metabolismo carbohydrate and starch in greenhouse-cucumber(dissertation).Hohhot:Inner Mongolia Agricultural University(in Chinese with English abstract)[夏永恒(2013).CO2加富条件下高温对温室黄瓜糖和淀粉代谢的影响(学位论文).呼和浩特:内蒙古农业大学]
Xu C(2013).Effects of high temperature stress on light energy distribution and antioxidant activy of eggplant seedlings(dissertation).Hangzhou:Zhejiang Agriculture and Forestry University(in Chinese with English abstract)[徐春香(2013).高温胁迫对茄子幼苗光能分配及抗氧化活性的影响(学位论文).杭州:浙江农林大学]
Xu W(2016).The role and mechanism of melatonin in regulating thermotolerance in tomato(dissertation).Hangzhou:Zhejiang University(in Chinese with English abstract)[须文(2016).褪黑素在调控番茄耐热性中的作用及机制(学位论文).杭州:浙江大学]
Xu XD,Sun Y,Guo XQ,et al(2010).Effects of exogenous melatonin on ascorbate metabolism system in cucumber seedlings under high temperature stress.Chin J Appl Ecol,21(10):2580-2586(in Chinese with English abstract)[徐向东,孙艳,郭晓芹等(2010).褪黑素对高温胁迫下黄瓜幼苗抗坏血酸代谢系统的影响.应用生态学报,21(10):2580-2586]
Xu XD,Sun Y,Guo XQ,et al(2011).Effect of exgenous melatonin on photosynthesis and chlorophyll fluorescence parameters in leaves of cucumber seedlings under high temperature stress.J Nucl Agr Sci,25(1):179-184(in Chinese with English abstract)[徐向东,孙艳,郭晓芹等(2011).高温胁迫下外源褪黑素对黄瓜幼苗光合作用及叶绿素荧光的影响.核农学报,25(1):179-184]
Xu XD,Sun Y,Sun B,et al(2010).Effects of exogenous melatonin on active oxygen metabolism in cucumber seedlings under high emperature stress.Chin J Appl Ecol,21(5):1295-1300(in Chinese with English abstract)[徐向东,孙艳,孙波等(2010).高温胁迫下外源褪黑素对黄瓜幼苗活性氧代谢的影响.应用生态学报,21(5):1295-1300]
Yu X,Li J,Xie Y,et al(2016).Effects of exogenous melatonin on photosynthesis and antioxidant activity of eggplant seedlings under high temperature stress.J Hunan Agr Univ-Nat Sci,42(5):496-499(in Chinese with English abstract)[余雪娜,李婧娴,谢永东等(2016).外源褪黑素对高温胁迫下茄子幼苗光合及抗氧化特性的影响.湖南农业大学学报(自然科学版),42(5):496-499]
Zeng Q,Xu Z,Zhang E,et al(2017).Effects of exogenous melatonin on physiological characteristics of cabbage seedlings under high temperature stress.North Hortic,20:12-17(in Chinese with English abstract)[曾庆栋,许忠民,张恩慧等(2017).外源褪黑素对高温胁迫下甘蓝幼苗生理特性的影响.北方园艺,20:12-17]
Zhang J,Li TL,Xu J(2008).Changes of carbohydrate metabolism of tomato leaves under high temperature in daytime in solar greenhouse.Acta Hortic Sin,35(4):529-534(in Chinese with English abstract)[张洁,李天来,徐晶.高昼温对日光温室番茄叶片碳水化合物代谢的影响.园艺学报,35(4):529-534]
Zhao N(2012).Effects of exogenous melatonin on carbohydrate and nitrate metabolism in cucumber seedling leaves under high temperature stress(dissertation).Yangling:Northwest A&F University(in Chinese with English abstract)[赵娜(2012).外源褪黑素对高温胁迫条件下黄瓜幼苗碳氮代谢的影响(学位论文).杨凌:西北农林科技大学]
Zhao Y,Wang D,Zhao X(2014).Resent advance on melatonin in plants.Acta Bot Boreal-Occident Sin,34(1):196-205(in Chinese with English abstract)[赵燕,王东华,赵曦阳(2014).植物中褪黑素的研究进展.西北植物学报,34(1):196-205]
Zhou H,Guo S,An Y,et al(2016).Exogenous spermidine delays chlorophyll metabolism in cucumber leaves(Cucumis sativus L.)under high temperature stress.Acta Physiol Plant,38(9):224
Zhu WM,Ding HD,Qi NM,et al(2005).Effects of Cd2+stress on ascorbate-glutathione cycle metabolism in tomato seedlings.Acta Agr Boreali-Sin,20(3):50-53(in Chinesewith English abstract)[朱为民,丁海东,齐乃敏等(2005).Cd2+胁迫对番茄幼苗抗坏血酸-谷胱甘肽循环代谢的影响.华北农学报,20(3):50-53]
Bian FE,Sun YJ,Niu YJ,et al(2017).Effects of root-applied melatonin on photosystem II in grape leaves under heat stress.Plant Physiol J,53(2):257-263(in Chinese with English abstract)[卞凤娥,孙永江,牛彦杰等(2017).高温胁迫下根施褪黑素对葡萄叶片叶绿素荧光特性的影响.植物生理学报,53(2):257-263]
Ding F,Liu B,Zhang S(2017).Exogenous melatonin ameliorates cold-induced damage in tomato plants.Sci Hortic,219:264-271
Gong B,Shi QH(2017).Review of melatonin in horticultural crops.Sci Agr Sin,50(12):2326-2337(in Chinese with English abstract)[巩彪,史庆华(2017).园艺作物褪黑素的研究进展.中国农业科学,50(12):2326-2337]
Guo XX,Li XF,Zhu HF,et al(2015).Effects of waterlogging stress on ascorbate-glutathione cycle in Brassica campetris ssp.chinensis.Plant Physiol J,51(12):2181-2187(in Chinese with English abstract)[郭欣欣,李晓锋,朱红芳等(2015).淹水胁迫对不结球白菜抗坏血酸-谷胱甘肽循环的影响.植物生理学报,51(12):2181-2187]
Hu Z,Fan J,Xie Y,et al(2016).Comparative photosynthetic and metabolic analyses reveal mechanism of improved cold stress tolerance in bermudagrass by exogenous melatonin.Plant Physiol Bioch,100:94-104
Jiang C,Zu C(2015).Advances in melatonin and its roles in abiotic stress resistance in plants.Biotechnol Bull,31(4):47-55(in Chinese with English abstract)[姜超强,祖朝龙(2015).褪黑素与植物抗逆性研究进展.生物技术通报,31(4):47-55]
Lian Y,Liu F,Tian S,et al(2017).Advances of research on genetics and breeding of eggplant during“the Twelfth Five-Year Plan”in China.China Veget,2:14-22(in Chinese)[连勇,刘富中,田时炳等(2017).“十二五”我国茄子遗传育种研究进展.中国蔬菜,2:14-22]
Liu J,Wang W,Wang L,et al(2015).Exogenous melatonin improves seedling health index and drought tolerance in tomato.Plant Growth Regul,77:317-326
Lorenzen JH,Lafra AM.Effect of heat stress on enzymes that affect sucrose levels in potato shoots.J Am Soc Hortic Sci,121:1152-1156
Osório ML,Osório J,Romano A(2013).Photosynthesis,energy partitioning,and metabolic adjustments of the endangered Cistaceae species Tuberaria major under high temperature and drought.Photosynthetica,51(1):75-84
Sang QQ,Shu S,Shan X,et al(2016).Effects of exogenous spermidine on antioxidant system of tomato seedlings exposed to high temperature stress.Russ J Plant Physiol,63(5):645-655
Shi H,Tan DX,Reiter RJ,et al(2015).Melatonin induces class A1 heat-shock factors(HSFA1s)and their possible involvement of thermotolerance in Arabidopsis.J Pineal Res,58(3):335-342
Suleman P,Redha A,Afzal M,et al(2012).Temperature-induced changes of malondialdehyde,heat-shock proteins in relation to chlorophyll fluorescence and photosynthesis in Conocarpus lancifolius(Engl.).Acta Physiol Plant,35:1223-1231
Sun SN,Wang Q,Sun CC,et al(2017).Response and adaptation of photosynthesis of cucumber seedlings to high temperature stress.Chin J Appl Ecol,28(5):1603-1610(in Chinesewith English abstract)[孙胜楠,王强,孙晨晨等(2017).黄瓜幼苗光合作用对高温胁迫的响应与适应.应用生态学报,28(5):1603-1610]
Sun Y,Guo S,Xu G(2015).Effect of root-zone hypoxia stress on carbohydrate contents of muskmelon seedlings.Acta Bot Boreal-Occident Sin,35(1):168-174(in Chinese with English abstract)[孙艳军,郭世荣,徐刚(2015).根际低氧胁迫对网纹甜瓜幼苗植株碳水化合物含量的影响.西北植物学报,35(1):168-174]
Tan DX,Manchester LC,Helton P,et al(2007).Phytoremediative capacity of plants enriched with melatonin.Plant Signal Behav,2:514-516
Wang R,Yang XL,Xu H,et al(2016).Research progress of melatonin biosynthesis and metabolism in higher plants.Plant Physiol J,52(5):615-627(in Chinese with English abstract)[王蕊,杨小龙,须晖等(2016).高等植物褪黑素的合成和代谢研究进展.植物生理学报,52(5):615-627]
Wu X,Zhu ZW,Zhang AD,et al(2017).Effects of exogenous melatonin on the growth,photosynthesis and antioxidant system in eggplant(Solanum melongena L.)seedlings under low temperature stress.Acta Bot Boreal-Occident Sin,37(12):2427-2434(in Chinese with English abstract)[吴雪霞,朱宗文,张爱冬等(2017).外源褪黑素对低温胁迫下茄子幼苗生长及其光合作用和抗氧化系统的影响.西北植物学报,37(12):2427-2434]
Wu XX,Zhu YL,Zhu WM,et al(2006).Physiological effects of exogenous nitric oxide in tomato seedlings under NaCl stress.Sci Agr Sin,39(3):575-581(in Chinese with English abstract)[吴雪霞,朱月林,朱为民等(2006).外源一氧化氮对NaCl胁迫下番茄幼苗生理影响.中国农业科学,39(3):575-581]
Xia Y(2013).The effects of elevated CO2 with high temperature stress on the metabolismo carbohydrate and starch in greenhouse-cucumber(dissertation).Hohhot:Inner Mongolia Agricultural University(in Chinese with English abstract)[夏永恒(2013).CO2加富条件下高温对温室黄瓜糖和淀粉代谢的影响(学位论文).呼和浩特:内蒙古农业大学]
Xu C(2013).Effects of high temperature stress on light energy distribution and antioxidant activy of eggplant seedlings(dissertation).Hangzhou:Zhejiang Agriculture and Forestry University(in Chinese with English abstract)[徐春香(2013).高温胁迫对茄子幼苗光能分配及抗氧化活性的影响(学位论文).杭州:浙江农林大学]
Xu W(2016).The role and mechanism of melatonin in regulating thermotolerance in tomato(dissertation).Hangzhou:Zhejiang University(in Chinese with English abstract)[须文(2016).褪黑素在调控番茄耐热性中的作用及机制(学位论文).杭州:浙江大学]
Xu XD,Sun Y,Guo XQ,et al(2010).Effects of exogenous melatonin on ascorbate metabolism system in cucumber seedlings under high temperature stress.Chin J Appl Ecol,21(10):2580-2586(in Chinese with English abstract)[徐向东,孙艳,郭晓芹等(2010).褪黑素对高温胁迫下黄瓜幼苗抗坏血酸代谢系统的影响.应用生态学报,21(10):2580-2586]
Xu XD,Sun Y,Guo XQ,et al(2011).Effect of exgenous melatonin on photosynthesis and chlorophyll fluorescence parameters in leaves of cucumber seedlings under high temperature stress.J Nucl Agr Sci,25(1):179-184(in Chinese with English abstract)[徐向东,孙艳,郭晓芹等(2011).高温胁迫下外源褪黑素对黄瓜幼苗光合作用及叶绿素荧光的影响.核农学报,25(1):179-184]
Xu XD,Sun Y,Sun B,et al(2010).Effects of exogenous melatonin on active oxygen metabolism in cucumber seedlings under high emperature stress.Chin J Appl Ecol,21(5):1295-1300(in Chinese with English abstract)[徐向东,孙艳,孙波等(2010).高温胁迫下外源褪黑素对黄瓜幼苗活性氧代谢的影响.应用生态学报,21(5):1295-1300]
Yu X,Li J,Xie Y,et al(2016).Effects of exogenous melatonin on photosynthesis and antioxidant activity of eggplant seedlings under high temperature stress.J Hunan Agr Univ-Nat Sci,42(5):496-499(in Chinese with English abstract)[余雪娜,李婧娴,谢永东等(2016).外源褪黑素对高温胁迫下茄子幼苗光合及抗氧化特性的影响.湖南农业大学学报(自然科学版),42(5):496-499]
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