耐寒和非耐寒橡胶树幼苗叶片抗氧化及抗坏血酸-谷胱甘肽循环对低温胁迫的反应差异
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摘要
【目的】研究低温胁迫下,耐寒和非耐寒橡胶树幼苗叶片抗氧化系统及抗坏血酸-谷胱甘肽循环的反应差异。【方法】以橡胶树耐寒无性系‘云研77-4’和非耐寒无性系‘热垦501’为材料进行低温胁迫试验。在橡胶树幼苗生长至2蓬叶时,进行4℃低温胁迫处理及28℃最适温度处理,设定3个时间梯度:1、12、24 h。处理完成后取第二轮叶片测定其抗氧化酶活性以及抗坏血酸-谷胱甘肽循环变化。【结果】随着低温胁迫处理时间的延长,2个无性系的H2O2含量均呈现先增加后降低的趋势,耐寒无性系‘云研77-4’的增减幅度大于非耐寒无性系‘热垦501’;与对照相比,2个无性系的SOD、POD、CAT等抗氧化酶活力以及As A含量、GSH含量均呈现出下降-上升-下降的趋势,‘云研77-4’的抗氧化酶活性以及As A含量、GSH含量高于‘热垦501’;GSH-PX活力呈现先下降后上升的变化趋势,与GSH含量的变化趋势相反。【结论】研究结果显示耐寒和非耐寒无性系橡胶树叶片抗氧化和抗坏血酸-谷胱甘肽循环系统在低温胁迫下的反应差异,表明叶片抗氧化及抗坏血酸–谷胱甘肽循环系统与橡胶树幼苗耐寒性密切相关。
【Objective】The aim of this study was to compare the response difference of antioxidant and ascorbate-glutathione cycle system in the leaves of cold tolerant and non-cold tolerant rubber seedlings to low temperature stress.【Method】The experiment was conducted using the leaves of cold-resistant'Yunyan 77-4'and cold-sensitive 'Reken 501 'as materials. Cold treatments( 4 ℃) were performed when the seedlings developed two extension units and the leaves were completely mature,and temperature treatments( 28 ℃) were regarded as controls. The leaf samples of two rubber tree clones were collected for determining the antioxidant enzyme activity and ascorbate glutathione cycle after 1,12,and 24 hours,respectively.【Result】The results showed that under low temperature,the H2 O2 contents of two rubber tree clones showed increased at first,and then decreased with the extension of treatment time,and the range of increase or decrease in cold-resistant'Yunyan 77-4'was more greater than the cold-sensitive'Reken 501'. Compared with controls,the activities of superoxide dismutase( SOD),peroxidase( POD) and catalase( CAT),the contents of ascorbic acid( As A) and glutathione( GSH) under low temperature in the two rubber clones were decreased at first,then increased and decreased in the end. And the activities of SOD,POD,CAT and the contents of As A and GSH of'Yunyan 77-4'were more than that of'Reken 501',whereas the activity of glutathione peroxidase( GSH-PX) was increased at first and then decreased,on the contrary with the trends of GSH content. 【Conclusion】The response difference of antioxidant and ascorbate-glutathione cycle system in the two rubber tree clones under low temperature stress indicated that antioxidant activity and ascorbate glutathione cycle were closely related to freezing resistance of rubber tree seedling.
【Objective】The aim of this study was to compare the response difference of antioxidant and ascorbate-glutathione cycle system in the leaves of cold tolerant and non-cold tolerant rubber seedlings to low temperature stress.【Method】The experiment was conducted using the leaves of cold-resistant'Yunyan 77-4'and cold-sensitive 'Reken 501 'as materials. Cold treatments( 4 ℃) were performed when the seedlings developed two extension units and the leaves were completely mature,and temperature treatments( 28 ℃) were regarded as controls. The leaf samples of two rubber tree clones were collected for determining the antioxidant enzyme activity and ascorbate glutathione cycle after 1,12,and 24 hours,respectively.【Result】The results showed that under low temperature,the H2 O2 contents of two rubber tree clones showed increased at first,and then decreased with the extension of treatment time,and the range of increase or decrease in cold-resistant'Yunyan 77-4'was more greater than the cold-sensitive'Reken 501'. Compared with controls,the activities of superoxide dismutase( SOD),peroxidase( POD) and catalase( CAT),the contents of ascorbic acid( As A) and glutathione( GSH) under low temperature in the two rubber clones were decreased at first,then increased and decreased in the end. And the activities of SOD,POD,CAT and the contents of As A and GSH of'Yunyan 77-4'were more than that of'Reken 501',whereas the activity of glutathione peroxidase( GSH-PX) was increased at first and then decreased,on the contrary with the trends of GSH content. 【Conclusion】The response difference of antioxidant and ascorbate-glutathione cycle system in the two rubber tree clones under low temperature stress indicated that antioxidant activity and ascorbate glutathione cycle were closely related to freezing resistance of rubber tree seedling.
引文
[1]安泽伟,陈根辉,程汉,等.橡胶树冷应答转录组c DNA-AFLP分析[J].林业科学,2010(3):62-67.
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[11]Rao P S,Saraswathyamma C K,Sethuraj M R.Studies on the relationship between yield and meteorological parameters of para rubber tree(Hevea brasiliensis)[J].Agricultural&Forest Meteorology,1998,90(3):235-245.
[12]刘世红,田耀华,魏丽萍,等.西双版纳30个橡胶树品种的低温半致死温度及低温对抗氧化系统的影响[J].植物生理学报,2011,47(5):505-511.
[13]刘鸿先,曾韶西,李平.植物抗寒性与酶系统多态性的关系[J].植物生理学通讯,1981,6(6):6-11.
[14]杨慧菊,郭华春.马铃薯幼苗低温胁迫的生理响应及品种耐寒性综合评价[J].西南农业学报,2016,29(11):2560-2566.
[15]张怡,路铁刚.植物中的活性氧研究概述[J].生物技术进展,2011,1(4):242-248.
[16]赵卫星,刘喜存,李晓慧,等.甜瓜幼苗对逆境胁迫的生理响应及抗逆性综合评价[J].西南农业学报,2017,30(2):322-326.
[17]Gill S S,Tuteja N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiol Biochem,2010,48(12):909-930.
[18]刘丰娇,蔡冰冰,孙胜楠,等.富氢水浸种增强黄瓜幼苗耐冷性的作用及其生理机制[J].中国农业科学,2017,50(5):881-889.
[19]Kidd P B,Young M W,Siggia E D.Temperature compensation and temperature sensation in the circadian clock[J].Proceedings of the National Academy of Sciences of the United States of America,2015,112(46):6284-6292.
[20]Seo P J,Mas P.Stressing the role of the plant circadian clock[J].Trends in Plant Science,2015,20(4):230-237.
[2]Vinod K K,Meena J R,Reddy Y A N,et al.Ontogenetic variations in flush development are indicative of low temperature tolerance in Hevea brasiliensis clones[J].Annals of Forest Research,2010,53(2):95-105.
[3]刘少军,周广胜,房世波.中国橡胶树种植气候适宜性区划[J].中国农业科学,2015,48(12):2335-2345.
[4]张勇,李芹,王树明,等.滇东南植胶区2013/2014年冬春橡胶树寒害调研报告[J].热带农业科学,2015,35(2):36-41.
[5]王祥军,李维国,高新生,等.巴西橡胶树响应低温逆境的生理特征及其调控机制[J].植物生理学报,2012,48(4):318-324.
[6]Loudet O,Hasegawa P M.Abiotic stress,stress combinations and crop improvement potential[J].Plant Journal,2017,90(5):837-838.
[7]李琲琲,刘志萍,张凤英,等.耐盐和非耐盐大麦幼苗叶片抗氧化及抗坏血酸-谷胱甘肽循环系统对Na Cl胁迫的反应差异[J].植物营养与肥料学报,2017,23(3):712-720.
[8]莫廷辉,黄俊生,张影波,等.橡胶树不同品种抗寒性综合鉴定[J].热带作物学报,2009,30(5):637-643.
[9]敖硕昌,和丽岗,肖桂秀,等.橡胶树高产抗寒材料云研77-2、云研77-4的选育[J].云南热作科技,1998(2):4-9.
[10]吴绍华,王建霄,陈月异,等.巴西橡胶树Hb MAPK1基因的克隆及表达分析[J].热带作物学报,2015,36(9):1580-1586.
[11]Rao P S,Saraswathyamma C K,Sethuraj M R.Studies on the relationship between yield and meteorological parameters of para rubber tree(Hevea brasiliensis)[J].Agricultural&Forest Meteorology,1998,90(3):235-245.
[12]刘世红,田耀华,魏丽萍,等.西双版纳30个橡胶树品种的低温半致死温度及低温对抗氧化系统的影响[J].植物生理学报,2011,47(5):505-511.
[13]刘鸿先,曾韶西,李平.植物抗寒性与酶系统多态性的关系[J].植物生理学通讯,1981,6(6):6-11.
[14]杨慧菊,郭华春.马铃薯幼苗低温胁迫的生理响应及品种耐寒性综合评价[J].西南农业学报,2016,29(11):2560-2566.
[15]张怡,路铁刚.植物中的活性氧研究概述[J].生物技术进展,2011,1(4):242-248.
[16]赵卫星,刘喜存,李晓慧,等.甜瓜幼苗对逆境胁迫的生理响应及抗逆性综合评价[J].西南农业学报,2017,30(2):322-326.
[17]Gill S S,Tuteja N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiol Biochem,2010,48(12):909-930.
[18]刘丰娇,蔡冰冰,孙胜楠,等.富氢水浸种增强黄瓜幼苗耐冷性的作用及其生理机制[J].中国农业科学,2017,50(5):881-889.
[19]Kidd P B,Young M W,Siggia E D.Temperature compensation and temperature sensation in the circadian clock[J].Proceedings of the National Academy of Sciences of the United States of America,2015,112(46):6284-6292.
[20]Seo P J,Mas P.Stressing the role of the plant circadian clock[J].Trends in Plant Science,2015,20(4):230-237.