摘要
目的:采用超高效液相色谱法(UPLC)测定豌豆尖中类黄酮水解产物槲皮素和山奈酚的含量。方法:采用Waters ACQUITY UPLC BEH Shield RP18色谱柱(100 mm×2.1 mm,1.7μm),以乙腈-0.1%冰醋酸水溶液(60:40)为流动相,流速为0.4 m L/min,槲皮素检测波长为371 nm,山奈酚检测波长为366 nm,柱温为30℃,进样量2.0μL。结果:槲皮素和山奈酚在1.5 min时间内达到基线分离,分别在(0.03~0.18)mg/m L、(0.015~0.15)mg/m L范围内与峰面积线性关系良好,平均回收率分别为101.37%、100.64%,RSD分别为0.84%、0.37%。结论:该方法快速、准确、稳定,可用于豌豆尖中槲皮素和山奈酚的含量测定。
Objective: To establish an UPLC method for simultaneous determination of quercetin and kaempferol in flavonoids hydrolysate from pea shoots. Methods: The sample solution was separated on a Waters ACQUITY UPLC BEH Shield RP18(100 mm×2.1 mm, 1.7 μm) column with acetonitrile-0.1% glacial acid solution(60:40) as mobile phase at a flow rate of 0.4 m L/min. The detection wavelength of quercetin was set at 371 nm and kaempferol's detection wavelength was set at 366 nm, the column temperature was set at 30 ℃ and the injection volume was 2 μL. Results: Quercetin and kaempferol were well separated in 1.5 minutes and had good linear relationship within the range of(0.03~0.18)mg/m L and(0.015~0.15)mg/m L respectively. The average recovery rate of quercetin and kaempferol were 101.37% and 100.64% respectively, the RSD were 0.84% and 0.37% respectively. Conclusion: The method is fast, accurate and stable for the determination of quercetin and kaempferol in pea shoots.
引文
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