三七根茎中的1个新达玛烷型三萜皂苷
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摘要
目的研究三七Panaxnotoginseng中的微量皂苷成分,为三七化学成分的系统研究、质量控制及安全性评价提供基础。方法综合利用MCI和ODS柱色谱以及制备型高效液相色谱等技术进行分离纯化,通过谱学数据分析以及与文献比对进行化合物结构鉴定。结果从三七根茎中共分离得到12个单体化合物,分别鉴定为三七皂苷P1(1)、三七皂苷T5(2)、人参皂苷Rk3(3)、人参皂苷Rh4(4)、三七皂苷T3(5)、20(S)-原人参三醇(6)、达玛-20(21),24-二烯-3β,6α,12β-三醇(7)、人参皂苷Rg3(8)、七叶胆皂苷XIII(9)、人参皂苷Rk1(10)、人参皂苷Rg5(11)、20(S)-人参皂苷Rh2(12)。结论化合物1为1个新的达玛烷型三萜皂苷。
Objective To study the minor triterpenoid saponins from the roots of Panax notoginseng, which provided basis for the systematic research, quality control and safety evaluation of P. notoginseng. Methods The compounds were isolated and purified by MCI resin, ODS, along with Preparative-HPLC, and the structures were identified by spectroscopic analysis, and comparing with the pubished literature values. Results Twelve monomeric compounds isolated from the roots of P. notoginseng, were identified as notoginsenoside P1(1), notoginsenoside T5(2), ginsenoside Rk3(3), ginsenoside Rh4(4), notoginsenoside T3(5),20(S)-protopanaxatriol(6), dammar 20(21),24-diene-3β,6α,12β-triol(7), ginsenoside Rg3(8), gypenoside XIII(9), ginsenoside Rk1(10), ginsenoside Rg5(11), and 20(S)-ginsenoside Rh2(12). Conclusion Compound 1 is a new dammarane-type triterpenoid saponin.
Objective To study the minor triterpenoid saponins from the roots of Panax notoginseng, which provided basis for the systematic research, quality control and safety evaluation of P. notoginseng. Methods The compounds were isolated and purified by MCI resin, ODS, along with Preparative-HPLC, and the structures were identified by spectroscopic analysis, and comparing with the pubished literature values. Results Twelve monomeric compounds isolated from the roots of P. notoginseng, were identified as notoginsenoside P1(1), notoginsenoside T5(2), ginsenoside Rk3(3), ginsenoside Rh4(4), notoginsenoside T3(5),20(S)-protopanaxatriol(6), dammar 20(21),24-diene-3β,6α,12β-triol(7), ginsenoside Rg3(8), gypenoside XIII(9), ginsenoside Rk1(10), ginsenoside Rg5(11), and 20(S)-ginsenoside Rh2(12). Conclusion Compound 1 is a new dammarane-type triterpenoid saponin.
引文
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[2]王莹,禇扬,李伟,等.三七中皂苷成分及其药理作用的研究进展[J].中草药,2015,46(9):1381-1392.
[3]田会东,郭丽娜,王单单,等.三七主要活性成分作用机制的网络药理学研究[J].药物评价研究,2019,42(1):72-77.
[4]Teng R W,Li H Z,Wang D Z,et al.Hydrolytic reaction of plant extracts to generate molecular diversity:New dammarane glycosides from the mild acid hydrolysate of root saponins of Panax notoginseng[J].Helv Chim Acta,2004,87(5):1270-1278.
[5]Park I H,Kim N Y,Han S B,et al.Three new dammarane glycosides from heat processed ginseng[J].Arch Pharm Res,2002,25(4):428-432.
[6]杨秀伟,李珂珂,周琪乐.人参茎叶中1个新皂苷20(S)-人参皂苷Rf2[J].中草药,2015,46(21):3137-3145.
[7]Ma S G,Jiang Y T,Song S J,et al.Alkaline-degradation products of ginsenosides from leaves and stems of Panax quinquefolium[J].Acta Pharm Sin,2005,40(10):924-930.
[8]李海舟,张颖君,杨崇仁.三七叶化学成分的进一步研究[J].天然产物研究与开发,2006,18(4):549-554.