抗阻训练对衰老大鼠线粒体介导腓肠肌细胞凋亡通路相关因子表达影响的研究
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摘要
目的:探讨抗阻训练对衰老大鼠线粒体介导腓肠肌细胞凋亡通路相关因子表达的影响。方法:以雄性SD大鼠为研究对象,饲养至18月龄后通过8周抗阻训练干预,分别以无负重,30%、50%、70%最大负重进行间歇跑台运动,坡度为35°,跑速15 m/min,隔天1次。8周末检测腓肠肌ROS、Cyt c和Bcl-2 mRNA表达,并与安静组比较。结果:8周抗阻训练使衰老大鼠腓肠肌ROS、Cyt c减少,Bcl-2mRNA表达增加,其中30%、50%最大负重抗阻训练两组大鼠ROS下降具有统计学意义(P<0.01);30%、50%最大负重抗阻训练两组大鼠腓肠肌Bcl-2 mRNA表达增加具有统计学意义(P<0.01,P<0.05);无负重,30%、50%最大负重抗阻训练3组大鼠腓肠肌Cyt c减少具有统计学意义(P<0.05,P<0.01)。结论:低、中等负重抗阻训练能下调衰老过程中大鼠腓肠肌ROS、Cyt c,促进Bcl-2 mRNA的表达,起到改善线粒体功能的作用,从而预防、延迟细胞凋亡的发生。
Objective To study the effects of resistance training on the factors related to mitochondria-mediated apoptosis pathway in gastrocnemius of aging rats.Methods 18-month old male SD rats underwent 8-week treadmill interval running(at the speed of 15m/min and slope of 35 °)with resistance of 0%,30%,50%,and 70%maximum weight,respectively.The expressions of gastrocnemius R0 S,Cyt c and Bcl-2 mRNA were tested after 8-week training and compared with the sedentary control rats.Results As compared with the control group,the content of gastrocnemius ROS,Cyt c in resistance training groups decreased,especially ROS in 30%and 50%maximum weight resistance training groups(P<0.01)and Cyt c in 0%,30%,50%maximum weight resistance training groups(P< 0.05,P< 0.01),and the Bcl-2 mRNA expression in resistance training groups increased,especially in 30%and 50%maximum weight resistance training groups(P < 0.01,P < 0.05).Conclusion Resistance training with low and moderate intensity lowers the content of ROS and Cyt c and enhances the expression of Bcl-2mRNA in gastrocnemius during the aging process,presenting the improvement of mitochondrial function and postponement of apoptosis occurrence.
Objective To study the effects of resistance training on the factors related to mitochondria-mediated apoptosis pathway in gastrocnemius of aging rats.Methods 18-month old male SD rats underwent 8-week treadmill interval running(at the speed of 15m/min and slope of 35 °)with resistance of 0%,30%,50%,and 70%maximum weight,respectively.The expressions of gastrocnemius R0 S,Cyt c and Bcl-2 mRNA were tested after 8-week training and compared with the sedentary control rats.Results As compared with the control group,the content of gastrocnemius ROS,Cyt c in resistance training groups decreased,especially ROS in 30%and 50%maximum weight resistance training groups(P<0.01)and Cyt c in 0%,30%,50%maximum weight resistance training groups(P< 0.05,P< 0.01),and the Bcl-2 mRNA expression in resistance training groups increased,especially in 30%and 50%maximum weight resistance training groups(P < 0.01,P < 0.05).Conclusion Resistance training with low and moderate intensity lowers the content of ROS and Cyt c and enhances the expression of Bcl-2mRNA in gastrocnemius during the aging process,presenting the improvement of mitochondrial function and postponement of apoptosis occurrence.
引文
[1]Edstrom E,Altun M,Hagglund M,et al.Atrogin-l/MAFBx and MuRFl are downregulated in aging-related loss of skeletal muscle.J Gerontol A Biol Sci Med Sci,2006,61(7):663-674.
[2]闫万军,赵斌,刘丰彬,等.负重跑训练对老龄大鼠肌肉丢失的影响.中国体育科技,2009,45(4):120-124.
[3]Hayes A.Cribb PJ.Effect of whey protein isolate on strength,body composition and muscle hypertrophy during resistance training.Curr Opin Clin Nutr Metab Care,2008,11(1):40-44.
[4]詹启敏.分子肿瘤学.北京:人民卫生出版社,2005:38-103.
[5]Grandemange S,Herzig S,Martinou JC.Mitochondrial dynamics and cancer.Semin Cancer Biol,2009,19(1):50-56.
[6]Van Houten B.Woshner V,Santos JH.Role of mitochondrial DNA in toxic responses to oxidative stress.DNA Repair(Amst),2006,5(2):145-152.
[7]曾季平,王立祥,夏文,等.活性氧在氯化锰致PC12细胞凋亡中的作用.中华职业病劳动卫生杂志,2006,24(3):157-159.
[8]Dirks AJ,Leeu WE,Nburgh C.The role of apoptosis in agerelated skeletal muscle atrophy.Sports Med,2005,35(6):473-483.
[9]Li M,Hou G,Huang D-N,et al.Muscle atrophy and oxidative stress.Chin J Biochem Mol Biol,2009,25(5).-415-420.
[10]漆正堂,贺杰,张媛,等.65%~75%最大强度的耐力运动对老龄小鼠骨骼肌线粒体氧化应激与膜电位的影响.体育科学,2010,10(30):46-51.
[11]Bo H,Jiang N,Ma GD,et al.Regulation of mitochondrial uncoupling respiration during exercise in rat heart:Role of reactive oxygen species(ROS)and uncoupling protein2.Free Radic Biol Med,2008,44(7):1373-1381.
[12]Van Houten B.Woshner V,Santos JH.Role of mitochondrial DNA in toxic responses to oxidative stress.DNA Repair(Amst),2006,5(2):145-152.
[13]Song W.Kwak HB.Lawler JM.Exercise training attenuates age-induced changes in apoptotic signaling in rat skeletal muscle.Antioxid Redox Signal,2006,8(3-4):517-528.
[14]张晓妮,冷传礼,宋扬.IP6诱导HT-29细胞凋亡中线粒体通路的实验研究.齐鲁医学杂志,2009,8,24(4):319-323.
[15]Siu PM.Bryner RW.Maryn JK,et al.Apoptotic adaptations from exercise training in skeletal and cardiac muscles.Faseb,20O4,18(lO):1150-1152.
[2]闫万军,赵斌,刘丰彬,等.负重跑训练对老龄大鼠肌肉丢失的影响.中国体育科技,2009,45(4):120-124.
[3]Hayes A.Cribb PJ.Effect of whey protein isolate on strength,body composition and muscle hypertrophy during resistance training.Curr Opin Clin Nutr Metab Care,2008,11(1):40-44.
[4]詹启敏.分子肿瘤学.北京:人民卫生出版社,2005:38-103.
[5]Grandemange S,Herzig S,Martinou JC.Mitochondrial dynamics and cancer.Semin Cancer Biol,2009,19(1):50-56.
[6]Van Houten B.Woshner V,Santos JH.Role of mitochondrial DNA in toxic responses to oxidative stress.DNA Repair(Amst),2006,5(2):145-152.
[7]曾季平,王立祥,夏文,等.活性氧在氯化锰致PC12细胞凋亡中的作用.中华职业病劳动卫生杂志,2006,24(3):157-159.
[8]Dirks AJ,Leeu WE,Nburgh C.The role of apoptosis in agerelated skeletal muscle atrophy.Sports Med,2005,35(6):473-483.
[9]Li M,Hou G,Huang D-N,et al.Muscle atrophy and oxidative stress.Chin J Biochem Mol Biol,2009,25(5).-415-420.
[10]漆正堂,贺杰,张媛,等.65%~75%最大强度的耐力运动对老龄小鼠骨骼肌线粒体氧化应激与膜电位的影响.体育科学,2010,10(30):46-51.
[11]Bo H,Jiang N,Ma GD,et al.Regulation of mitochondrial uncoupling respiration during exercise in rat heart:Role of reactive oxygen species(ROS)and uncoupling protein2.Free Radic Biol Med,2008,44(7):1373-1381.
[12]Van Houten B.Woshner V,Santos JH.Role of mitochondrial DNA in toxic responses to oxidative stress.DNA Repair(Amst),2006,5(2):145-152.
[13]Song W.Kwak HB.Lawler JM.Exercise training attenuates age-induced changes in apoptotic signaling in rat skeletal muscle.Antioxid Redox Signal,2006,8(3-4):517-528.
[14]张晓妮,冷传礼,宋扬.IP6诱导HT-29细胞凋亡中线粒体通路的实验研究.齐鲁医学杂志,2009,8,24(4):319-323.
[15]Siu PM.Bryner RW.Maryn JK,et al.Apoptotic adaptations from exercise training in skeletal and cardiac muscles.Faseb,20O4,18(lO):1150-1152.