动物头部动脉及脑动脉血管铸型方法的建立
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摘要
采用新鲜绵羊头、兔头各10个,自制硬头软管灌注装置,通过缓压适量灌注方法、带颅骨及带头骨腐蚀方法和延长腐蚀时间等综合方法制作血管铸型标本.结果表明:试验获得了精美、完整的绵羊脑及脑外周血管铸型和兔头部动脉血管铸型,采用该方法可成功获得小型哺乳动物血管铸型标本.
This study was carried out to improve the experimental method for artery casting mould of small mammal head and brain and provide an excellent research solution for the investigation of animal head and brain vessels.After the fresh heads and brains from 10 sheep and 10 rabbits were prepared,the self-invented device,which was characterized by a hard tip and a soft tube,was adopted,and the comprehensive methods including high pressure and proper perfusion quantity method,corrosion containing skull method and elongating time corrosion method were used to make the vessel casting mould for head and brain arteries.The perfect,intact casting mould of head and periphery vessel of sheep were obtained,as well as the head artery casting mould of rabbits.On the base of a series of improvements in experimental methods,the vessel casting mould of small mammal could be successfully obtained.
This study was carried out to improve the experimental method for artery casting mould of small mammal head and brain and provide an excellent research solution for the investigation of animal head and brain vessels.After the fresh heads and brains from 10 sheep and 10 rabbits were prepared,the self-invented device,which was characterized by a hard tip and a soft tube,was adopted,and the comprehensive methods including high pressure and proper perfusion quantity method,corrosion containing skull method and elongating time corrosion method were used to make the vessel casting mould for head and brain arteries.The perfect,intact casting mould of head and periphery vessel of sheep were obtained,as well as the head artery casting mould of rabbits.On the base of a series of improvements in experimental methods,the vessel casting mould of small mammal could be successfully obtained.
引文
[1]李忠华,王兴梅.解剖学技术[M].2版.北京:人民卫生出版社,1997:123-137
[2]于频,刘正津.解剖学技术[M].北京:人民卫生出版社,1985:194-232
[3]程明亮,袁耀华.人体全身血管铸型标本的研制[J].解剖学研究,2004.26(3):239-240
[4]张卫东,郎庆玲.人血管的ABS铸型[J].佳木斯医学院学报,1995,18(2):7-8
[5]纪建松,龚建平,王天佑.两种铸型标本制作方法的比较[J].中国血液流变学杂志,2003,13(1):26-28
[6]洪辉文,吴坤成,李泽宇,等.全身动脉铸型制作方法的改进[J].中国临床解剖学杂志,2007,25(4):478-479
[7]张海,徐连第,殷利武.动物铸型标本的价值及研制中填充剂的选择[J].内蒙古师范大学学报,2010,23(11):154-156
[8]《家畜解剖标本制作法》编写组编.家畜解剖标本制作法[M].北京:农业出版社,1977:98-105
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[10]陈雄林,王琦,刘瑜琦,等.兔动脉铸型标本制作方法[J].九江学院学报,2006,3(6):77-78
[11]赵建红,俞红贤,刘英,等.绵羊支气管动脉立体铸型方法研究[J].甘肃农业大学学报,2007,6(3):38-40
[12]吴坤成,李泽宇,刘畅,等.脑血管铸型标本的设计与制作[J].解剖学杂志,2005,28(6):643-649
[13]吴坤成,李泽宇,刘畅,等.颅内外动脉铸型标本的设计与制作[J].中国临床解剖学杂志,2006,24(1):111-112
[14]秦智勇,陈衔城,马端,等.幼猪颈内动脉系统脑血管解剖特点与脑栓塞模型的制作[J].中华实验外科杂志,1999,16(1):1-4
[15]盛希忠,刘作勤.家猪头颈部血管解剖学研究及其在人脑血管病研究中的意义[J].中国比较医学杂志,2006,16(4):208-211
[2]于频,刘正津.解剖学技术[M].北京:人民卫生出版社,1985:194-232
[3]程明亮,袁耀华.人体全身血管铸型标本的研制[J].解剖学研究,2004.26(3):239-240
[4]张卫东,郎庆玲.人血管的ABS铸型[J].佳木斯医学院学报,1995,18(2):7-8
[5]纪建松,龚建平,王天佑.两种铸型标本制作方法的比较[J].中国血液流变学杂志,2003,13(1):26-28
[6]洪辉文,吴坤成,李泽宇,等.全身动脉铸型制作方法的改进[J].中国临床解剖学杂志,2007,25(4):478-479
[7]张海,徐连第,殷利武.动物铸型标本的价值及研制中填充剂的选择[J].内蒙古师范大学学报,2010,23(11):154-156
[8]《家畜解剖标本制作法》编写组编.家畜解剖标本制作法[M].北京:农业出版社,1977:98-105
[9]李鹏,宋泽龙,刘阳,等.家兔全身动脉铸型标本的制作[J].滨州医学院学报,2010,33(3):196-197
[10]陈雄林,王琦,刘瑜琦,等.兔动脉铸型标本制作方法[J].九江学院学报,2006,3(6):77-78
[11]赵建红,俞红贤,刘英,等.绵羊支气管动脉立体铸型方法研究[J].甘肃农业大学学报,2007,6(3):38-40
[12]吴坤成,李泽宇,刘畅,等.脑血管铸型标本的设计与制作[J].解剖学杂志,2005,28(6):643-649
[13]吴坤成,李泽宇,刘畅,等.颅内外动脉铸型标本的设计与制作[J].中国临床解剖学杂志,2006,24(1):111-112
[14]秦智勇,陈衔城,马端,等.幼猪颈内动脉系统脑血管解剖特点与脑栓塞模型的制作[J].中华实验外科杂志,1999,16(1):1-4
[15]盛希忠,刘作勤.家猪头颈部血管解剖学研究及其在人脑血管病研究中的意义[J].中国比较医学杂志,2006,16(4):208-211