干旱胁迫下热带樱花叶片与气孔形态变化特征研究
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摘要
为研究热带樱花在海南岛园林绿化中的推广与应用,针对其抗逆适应性进行干旱胁迫试验。研究设4个水分干旱梯度试验,采用改良指甲油印迹法测定其叶片气孔的形态特征,观察叶片形态变化。结果表明:(1)随着干旱时间延长,降低了其叶面积与叶片含水量,干旱处理30 d变化最明显;(2)随干旱程度的加重,叶片气孔密度、气孔周长等呈减小趋势;(3)干旱下气孔密度与其开放度有显著相关性(R2=0.038 3,p<0.05,N=36)。干旱条件对海南岛种植热带樱花有很大影响。
The purpose of this study is to do application and promotion of tropical cherry blossom in Hainan Island.The study set up 4 water drought gradient tests,using the improved method to determine the morphological characteristics of leaf stomata.The main test showed that:(1)Long time drought affected leaf area and leaf water content for the most obvious change of thirtieth days;(2)With the drought,stomatal density,stomatal perimeter decreased;(3)Under drought stress and stomatal density of openness has a significant correlation(R~2=0.038 3,p<0.05,N=36).The drought conditions had a great influence on the planting of tropical cherry blossoms in Hainan Island.
The purpose of this study is to do application and promotion of tropical cherry blossom in Hainan Island.The study set up 4 water drought gradient tests,using the improved method to determine the morphological characteristics of leaf stomata.The main test showed that:(1)Long time drought affected leaf area and leaf water content for the most obvious change of thirtieth days;(2)With the drought,stomatal density,stomatal perimeter decreased;(3)Under drought stress and stomatal density of openness has a significant correlation(R~2=0.038 3,p<0.05,N=36).The drought conditions had a great influence on the planting of tropical cherry blossoms in Hainan Island.
引文
[1]石培华,冷石林,梅旭荣.气孔导度、表面温度的环境响应模型研究[J].中国农业气象,1995(5):51-54.
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[3]张守仁,高荣孚.白杨派新无性系气孔生理生态特性的研究[J].生态学报,1998(4):24-27,29.
[4]王曙光,李中青,贾寿山,等.小麦叶片气孔性状与产量和抗旱性的关系[J].应用生态学报,2013,24(6):1 609-1 614.
[5]熊慧,马承恩,李乐,等.不同生境条件下蕨类和被子植物的气孔形态特征及其对光强变化的响应[J].植物生态学报,2014,38(8):868-877.
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[7]张庆华,曾祥国,向发云,等.干旱胁迫对草莓苗期叶片光合特性的影响[J].湖北农业科学,2016(23):6 147-6 150.
[8]张斌,周广柱,聂义丰,等.干旱胁迫对银杏幼苗叶片光合性状的影响[J].江苏农业科学,2016(5):202-205.
[9]李真真,张莉,李思,等.玉米叶片气孔及花环和维管束结构对水分胁迫的响应[J].应用生态学报,2014,25(10):2 944-2 950.
[10]王兰香,侯智慧,侯丽霞,等.H2O2介导的H2S产生参与干旱诱导的拟南芥气孔关闭[J].植物学报,2012,47(3):217-225.
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[13]Sven-Erik Jacobsen,Fulai Liu,Christian Richardt Jensen.Does root-sourced ABA play a role for regulation of stomata under drought in quinoa(Chenopodium quinoa Willd.)[J].Scientia Horticulturae,2009,122:281-287.
[14]张立荣,牛海山,汪诗平,等.增温与放牧对矮嵩草草甸4种植物气孔密度和气孔长度的影响[J].生态学报,2010,30(24):6 961-6 969.
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[16]李淑娴,吴沙沙,黄俊婷,等.粉花山扁豆种子萌发与贮藏特性[J].森林与环境学报,2015,35(4):364-369.
[17]邱琼,杨德军,陈显兵,等.不同处理方法对粉花山扁豆种子发芽率的影响[J].西部林业科学,2011(1):80-82.
[18]段云峰,王幼宁,李霞.一种获得叶片表皮观察气孔的简易方法及其应用[J].华北农学报,2008(S1):73-76.
[19]Seemann J R,Critchley C.Effects of salt stress on the growth,ion content,stomatal behaviour and photosynthetic capacity of a salt-sensitive species,Phaseolus vulgaris L.[J].Planta,1985,164(2):151-162.
[20]Dong Y,Wang C P,Han X,et al.A novel b HLH transcription factor Peb HLH35 from Populus euphratica confers drought tolerance through regulating stomatal development,photosynthesis and growth in Arabidopsis[J].Biochemical and Biophysical Research Communications,2014,450:453-458.
[21]田鑫,于广文.干旱胁迫对水稻叶片气孔密度的影响[J].辽宁农业科学,2010(2):26-28.
[22]Wang S G,Jia S S,D Z,et al.Mapping QTLs for stomatal density and size under drought stress in wheat(Triticum aestivum L.)[J].Journal of Integrative Agriculture,2016,15:1 955-1 967.
[23]刘世鹏,刘济明,曹娟云,等.干旱胁迫下枣树叶片表皮气孔分布及特征分析[J].安徽农业科学,2006(7):1 315-1 318.
[2]董树亭,胡昌浩,周关印.玉米叶片气孔导度、蒸腾和光合特性研究[J].玉米科学,1993(2):41-44.
[3]张守仁,高荣孚.白杨派新无性系气孔生理生态特性的研究[J].生态学报,1998(4):24-27,29.
[4]王曙光,李中青,贾寿山,等.小麦叶片气孔性状与产量和抗旱性的关系[J].应用生态学报,2013,24(6):1 609-1 614.
[5]熊慧,马承恩,李乐,等.不同生境条件下蕨类和被子植物的气孔形态特征及其对光强变化的响应[J].植物生态学报,2014,38(8):868-877.
[6]王晶晶,莫伟平,贾文锁,等.干旱条件下葡萄叶片气孔导度和水势与节位变化的关系[J].中国农业科学,2013,46(10):2 151-2 158.
[7]张庆华,曾祥国,向发云,等.干旱胁迫对草莓苗期叶片光合特性的影响[J].湖北农业科学,2016(23):6 147-6 150.
[8]张斌,周广柱,聂义丰,等.干旱胁迫对银杏幼苗叶片光合性状的影响[J].江苏农业科学,2016(5):202-205.
[9]李真真,张莉,李思,等.玉米叶片气孔及花环和维管束结构对水分胁迫的响应[J].应用生态学报,2014,25(10):2 944-2 950.
[10]王兰香,侯智慧,侯丽霞,等.H2O2介导的H2S产生参与干旱诱导的拟南芥气孔关闭[J].植物学报,2012,47(3):217-225.
[11]Yoko Tanaka,Tomoe Nose,Yusuke Jikumaru,et al.ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves[J].The Plant Journal:for Cell and Molecular Biology,2013,74(3):448-457.
[12]Mekonnen D W,Flügge U I,Ludewig F.Gamma-aminobutyric acid depletion affects stomata closure and drought tolerance of Arabidopsis thaliana[J].Plant Science An International Journal of Experimental Plant Biology,2016,245:25-34.
[13]Sven-Erik Jacobsen,Fulai Liu,Christian Richardt Jensen.Does root-sourced ABA play a role for regulation of stomata under drought in quinoa(Chenopodium quinoa Willd.)[J].Scientia Horticulturae,2009,122:281-287.
[14]张立荣,牛海山,汪诗平,等.增温与放牧对矮嵩草草甸4种植物气孔密度和气孔长度的影响[J].生态学报,2010,30(24):6 961-6 969.
[15]程政宁主编,《中国’99昆明世界园艺博览会园艺百科全书》编辑委员会编.中国’99昆明世界园艺博览会园艺百科全书[M].北京:中国林业出版社,2000:718.
[16]李淑娴,吴沙沙,黄俊婷,等.粉花山扁豆种子萌发与贮藏特性[J].森林与环境学报,2015,35(4):364-369.
[17]邱琼,杨德军,陈显兵,等.不同处理方法对粉花山扁豆种子发芽率的影响[J].西部林业科学,2011(1):80-82.
[18]段云峰,王幼宁,李霞.一种获得叶片表皮观察气孔的简易方法及其应用[J].华北农学报,2008(S1):73-76.
[19]Seemann J R,Critchley C.Effects of salt stress on the growth,ion content,stomatal behaviour and photosynthetic capacity of a salt-sensitive species,Phaseolus vulgaris L.[J].Planta,1985,164(2):151-162.
[20]Dong Y,Wang C P,Han X,et al.A novel b HLH transcription factor Peb HLH35 from Populus euphratica confers drought tolerance through regulating stomatal development,photosynthesis and growth in Arabidopsis[J].Biochemical and Biophysical Research Communications,2014,450:453-458.
[21]田鑫,于广文.干旱胁迫对水稻叶片气孔密度的影响[J].辽宁农业科学,2010(2):26-28.
[22]Wang S G,Jia S S,D Z,et al.Mapping QTLs for stomatal density and size under drought stress in wheat(Triticum aestivum L.)[J].Journal of Integrative Agriculture,2016,15:1 955-1 967.
[23]刘世鹏,刘济明,曹娟云,等.干旱胁迫下枣树叶片表皮气孔分布及特征分析[J].安徽农业科学,2006(7):1 315-1 318.