液质联用技术在中药质量控制中的应用
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摘要
中医药是中华民族宝贵的文化遗产,为中华民族的健康和繁衍做出了不可磨灭的贡献。中药独特的疗效及其对疑难杂症治疗的突出效果在世界范围内产生了积极的影响。但复杂的中药物质,对中药质量控制提出了很大的挑战,不利于中药走向现代化和国际化。在中药质量控制的众多分析检测手段中,液质联用技术具有选择性强、灵敏度高、分离能力好等优点而广泛应用。现以液质联用技术为切入点,主要阐述单味中药、中药复方制剂的主要成分或特征性成分的鉴别与含量测定,同时阐述部分非法添加成分检查方法等内容,拟为中药质量控制的进一步研究提供依据。
Traditional Chinese medicine( TCM) is a precious cultural heritage of China,which has made indelible contributions to the health and development of China. The effects of Chinese herbal medicine and its unique advantages on the treatment of difficult miscellaneous diseases have a positive impact in the world. However,Chinese herbal medicine has a unique theoretical system and complex material basis,which poses a great challenge to the quality control of Chinese herbal medicine,and it is not conducive to the modernization and internationalization of Chinese herbal medicine. Among many analytical methods for the quality control of Chinese herbal medicine,liquid chromatography coupled with mass spectrometry is widely used because of its high selectivity,high sensitivity and good separation ability. In this paper,liquid chromatography-mass spectrometry technology as the starting point,the determination and identification of the content or characteristic for single herb and compound Chinese herbal preparation were mainly elaborated. The determination of illegal addition inspection methods was also elaborated. The paper provided basic references for further research of quality control of Chinese herbal medicine.
Traditional Chinese medicine( TCM) is a precious cultural heritage of China,which has made indelible contributions to the health and development of China. The effects of Chinese herbal medicine and its unique advantages on the treatment of difficult miscellaneous diseases have a positive impact in the world. However,Chinese herbal medicine has a unique theoretical system and complex material basis,which poses a great challenge to the quality control of Chinese herbal medicine,and it is not conducive to the modernization and internationalization of Chinese herbal medicine. Among many analytical methods for the quality control of Chinese herbal medicine,liquid chromatography coupled with mass spectrometry is widely used because of its high selectivity,high sensitivity and good separation ability. In this paper,liquid chromatography-mass spectrometry technology as the starting point,the determination and identification of the content or characteristic for single herb and compound Chinese herbal preparation were mainly elaborated. The determination of illegal addition inspection methods was also elaborated. The paper provided basic references for further research of quality control of Chinese herbal medicine.
引文
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[4]Lan K,Zhang Y,Yang J,et al.Simple quality assessment approach for herbal extracts using high performance liquid chromatography-UV based metabolomics platform[J].J Chromatogr A,2010,1217(8):1414-8.
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[7]汪铜芳,张咏梅,李姗姗,等.高效液相色谱法测定川芎-白芷药对不同比例配伍中阿魏酸含量[J].辽宁中医药大学学报,2017,19(3):44-46.
[8]Chen J,Zhou J,Wei S,et al.Effect of a traditional Chinese medicine prescription Quzhuotongbi decoction on hyperuricemia model rats studied by using serum metabolomics based on gas chromatography-mass spectrometry[J].J Chromatogr B Analyt Technol Biomed Life Sci,2016,1026:272-278.
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[12]张加余,乔延江,张倩,等.液质联用技术在天然产物结构鉴定中的应用进展[J].药物分析杂志,2013,33(2):349-354.
[13]熊少祥,赵镇文,蒲丹,等.液相基质辅助激光解吸电离-傅里叶变换离子回旋共振质谱研究[J].分析化学,2004,32(1):14-18.
[14]尤娇娇,彭志红,韩凤梅.HPLC-MS/MS法同时测定平卧菊三七水提物中9种主要有效成分[J].中草药,2017,37(2):294-298.
[15]张晨曦,刘素香,赵艳敏,等.基于液质联用技术的连翘化学成分分析[J].中草药,2016,47(12):2053-2060.
[16]宋青青,赵云芳,张娜,等.生地黄HPLC指纹图谱的建立及其HPLC-ESI-MS分析[J].中草药,2016,47(23):4247-4252.
[17]董昕,刘敏,赵靖霞,等.传统中药川乌炮制前后的离子阱质谱研究[J].药学实践杂志,2009,27(5):349-352.
[18]徐昱婷,张学博,苏静,等.超高效液相色谱-串联四级杆质谱法测定中药材(饮片)中非法添加6种水溶性红色素通用方法的研究[J].世界科学技术-中医药现代化,2016,18(5):911-918.
[19]于泓,胡青,张甦,等.超高效液相色谱-四级杆-飞行时间串联质谱法检测中药及保健食品中21种非法添加的降脂类药物[J].食品安全质量检测学报,2016,7(7):2704-2709.
[20]张甦,胡青,孙健,等.液质联用检测中成药和保健食品中非法添加的48种抗生素类成分[J].中成药,2015,37(3):542-548.
[21]胡莹,吴啟南,郑啸,等.LC-MS/MS法同时测定金樱子中7种主要成分的含量[J].中药材,2016,39(12):2798-2800.
[22]吕亚男,谭光国.芎归汤主要化学成分的HPLC-TOF-MS分析[J].药学实践杂志,2015,33(1):49-52,71.
[23]刘昌孝,陈士林,肖小河,等.中药质量标志物(Q-Marker):中药产品质量控制的新概念[J].中草药,2016,47(9):1443-1457.
[24]周秀娟,李燕芳,陈莹,等.基于UPLC-Q Exactive四级杆-轨道阱液质联用法快速建立清热灵颗粒中潜在中药质量标志物(QMarker)成分库[J].中草药,2017,48(1):67-74.
[25]宋祥珍,李莉,张自强.非法添加止咳平喘类药物中药制剂的HPLC-MS/MS检测系统研究[J].药物分析杂志,2010,30(10):1822-1828.
[26]Shi F,Guo C,Gong L,et al.Application of a high resolution benchtop quadrupole-Orbitrap mass spectrometry for the rapidscreening,confirmation and quantification of illegal adulterated phosphodiesterase-5 inhibitorsin herbal medicines and dietary supplements[J].J Chromatogr A,2014,1344:91-98.
[2]刘荣霞,叶敏,果德安,等.中药质量控制研究的思路与方法[J].中国天然药物,2006,4(5):332-337.
[3]佘金燕,任平远,马小兵,等.中药材樟脑质量标准研究[J].亚太传统医药,2016,12(15):49-51.
[4]Lan K,Zhang Y,Yang J,et al.Simple quality assessment approach for herbal extracts using high performance liquid chromatography-UV based metabolomics platform[J].J Chromatogr A,2010,1217(8):1414-8.
[5]Zhu Q,Xu X,Huang Y,et al.Field enhancement sample stacking for analysis of organic acids in traditional Chinese medicine by capillary electrophoresis[J].J Chromatogr A,2012,1246:35-9.
[6]徐双双,王尉,贺天雨.高速逆流色谱法分离纯化红景天苷、葛根素和淫羊藿苷3种标准样品[J].食品安全质量检测学报,2016,7(11):4323-4328.
[7]汪铜芳,张咏梅,李姗姗,等.高效液相色谱法测定川芎-白芷药对不同比例配伍中阿魏酸含量[J].辽宁中医药大学学报,2017,19(3):44-46.
[8]Chen J,Zhou J,Wei S,et al.Effect of a traditional Chinese medicine prescription Quzhuotongbi decoction on hyperuricemia model rats studied by using serum metabolomics based on gas chromatography-mass spectrometry[J].J Chromatogr B Analyt Technol Biomed Life Sci,2016,1026:272-278.
[9]Kruve A,Rebane R,Kipper K,et al.Tutorial review on validation of liquid chromatography-mass spectrometry methods:part II[J].Anal Chim Acta,2015,870:8-28.
[10]张莉,张丽萍,孙成春.液-质联用技术在中药研究中的应用[J].解放军药学学报,2010,26(6):558-561.
[11]靳淑敏,韩茹,董振咏,等.液质联用技术在中药制剂分析中的应用进展[J].河北医药,2015,37(5):725-728.
[12]张加余,乔延江,张倩,等.液质联用技术在天然产物结构鉴定中的应用进展[J].药物分析杂志,2013,33(2):349-354.
[13]熊少祥,赵镇文,蒲丹,等.液相基质辅助激光解吸电离-傅里叶变换离子回旋共振质谱研究[J].分析化学,2004,32(1):14-18.
[14]尤娇娇,彭志红,韩凤梅.HPLC-MS/MS法同时测定平卧菊三七水提物中9种主要有效成分[J].中草药,2017,37(2):294-298.
[15]张晨曦,刘素香,赵艳敏,等.基于液质联用技术的连翘化学成分分析[J].中草药,2016,47(12):2053-2060.
[16]宋青青,赵云芳,张娜,等.生地黄HPLC指纹图谱的建立及其HPLC-ESI-MS分析[J].中草药,2016,47(23):4247-4252.
[17]董昕,刘敏,赵靖霞,等.传统中药川乌炮制前后的离子阱质谱研究[J].药学实践杂志,2009,27(5):349-352.
[18]徐昱婷,张学博,苏静,等.超高效液相色谱-串联四级杆质谱法测定中药材(饮片)中非法添加6种水溶性红色素通用方法的研究[J].世界科学技术-中医药现代化,2016,18(5):911-918.
[19]于泓,胡青,张甦,等.超高效液相色谱-四级杆-飞行时间串联质谱法检测中药及保健食品中21种非法添加的降脂类药物[J].食品安全质量检测学报,2016,7(7):2704-2709.
[20]张甦,胡青,孙健,等.液质联用检测中成药和保健食品中非法添加的48种抗生素类成分[J].中成药,2015,37(3):542-548.
[21]胡莹,吴啟南,郑啸,等.LC-MS/MS法同时测定金樱子中7种主要成分的含量[J].中药材,2016,39(12):2798-2800.
[22]吕亚男,谭光国.芎归汤主要化学成分的HPLC-TOF-MS分析[J].药学实践杂志,2015,33(1):49-52,71.
[23]刘昌孝,陈士林,肖小河,等.中药质量标志物(Q-Marker):中药产品质量控制的新概念[J].中草药,2016,47(9):1443-1457.
[24]周秀娟,李燕芳,陈莹,等.基于UPLC-Q Exactive四级杆-轨道阱液质联用法快速建立清热灵颗粒中潜在中药质量标志物(QMarker)成分库[J].中草药,2017,48(1):67-74.
[25]宋祥珍,李莉,张自强.非法添加止咳平喘类药物中药制剂的HPLC-MS/MS检测系统研究[J].药物分析杂志,2010,30(10):1822-1828.
[26]Shi F,Guo C,Gong L,et al.Application of a high resolution benchtop quadrupole-Orbitrap mass spectrometry for the rapidscreening,confirmation and quantification of illegal adulterated phosphodiesterase-5 inhibitorsin herbal medicines and dietary supplements[J].J Chromatogr A,2014,1344:91-98.