羊IL-2和OrfV-F1L基因疫苗联合免疫小鼠的免疫应答研究
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摘要
为评价pVAX1-IL-2和pVAX1-F1L联合免疫小鼠诱导的体液和细胞免疫应答,分别将p VAX1-F1L+pVAX1-IL-2、pVAX1-F1L、pVAX1空载体、PBS对照组通过后腿肌肉注射的方式免疫KM系小鼠,采用ELISA方法检测免疫小鼠血清中OrfV特异性抗体以及Th1型(IL-2、IFN-γ和TNF-α)、Th2型(IL-4、IL-6和IL-10)细胞因子;MTT法检测小鼠脾淋巴细胞增殖反应。结果显示,pVAX1-F1L+pVAX1-IL-2联合免疫组小鼠血清抗体效价及IL-2、IFN-γ和TNF-α细胞因子水平均显著高于pVAX1-F1L组,与p VAX1组、PBS组相比差异极显著(P<0.01);pVAX1-F1L+pVAX1-IL-2联合免疫组小鼠血清IL-4、IL-6和IL-10细胞因子水平均显著高于pVAX1组和PBS组,与pVAX1-F1L组相比差异不显著(P>0.05);且pVAX1-F1L+pVAX1-IL-2免疫组的脾淋巴细胞增殖水平高于pVAX1-F1L组、pVAX1组和PBS组(P<0.01)。结果表明,p VAX1-IL-2和pVAX1-F1L联合免疫小鼠,能诱导小鼠产生OrfV特异性体液免疫和细胞免疫,p VAX1-IL-2诱导的免疫反应以细胞免疫应答为主,且能促进Th1型细胞因子的分泌。
To evaluate the humoral and cellular immune responses induced in mice co-immunized with p VAX1-IL-2 and p VAX1-F1 L,KM mice were immunized with p VAX1-F1 L +p VAX1-IL-2,p VAX1-F1 L,p VAX1 empty vector and PBS control group by intramuscular injection of the hind leg,respectively.ELISA was used to detect Orf V-specific antibodies and Th1 type(IL-2,IFN-γ and TNF-α) and Th2 type(IL-4,IL-6 and IL-10)cytokines in the serum of the immunized mice.Splenic lymphocyte proliferation in the mice was detected by the MTT assay.In result,the serum antibody titers and levels of IL-2,IFN-γ,and TNF-α cytokines in the mice immunized with p VAX1-F1 L+p VAX1-IL-2 were significantly higher than those in the p VAX1-F1 L group,compared with the p VAX1 group and the PBS group.The difference between the groups was extremely significant(P<0.01).The levels of serum IL-4,IL-6,and IL-10 cytokines in the p VAX1-F1 L+p VAX1-IL-2 combined group were significantly higher than those in the p VAX1 group and the PBS group.Compared with the p VAX1-F1 L immunized group,the difference was not significant(P>0.05);and the p VAX1-F1 L+p VAX1-IL-2 immunized group had higher levels of spleen lymphocyte proliferation than the p VAX1-F1 L immunized group,the p VAX1 group and the PBS group(P<0.01).The mice immunized with p VAX1-IL-2 and p VAX1-F1 L could induce Orf V-specific humoral and cellular immunity.The immune responses induced by p VAX1-IL-2 were mainly cellular immune responses,which promoted the secretion of Th1 cytokines.The above-mentioned results provide the basis for further development of Orf V nucleic acid vaccines.
To evaluate the humoral and cellular immune responses induced in mice co-immunized with p VAX1-IL-2 and p VAX1-F1 L,KM mice were immunized with p VAX1-F1 L +p VAX1-IL-2,p VAX1-F1 L,p VAX1 empty vector and PBS control group by intramuscular injection of the hind leg,respectively.ELISA was used to detect Orf V-specific antibodies and Th1 type(IL-2,IFN-γ and TNF-α) and Th2 type(IL-4,IL-6 and IL-10)cytokines in the serum of the immunized mice.Splenic lymphocyte proliferation in the mice was detected by the MTT assay.In result,the serum antibody titers and levels of IL-2,IFN-γ,and TNF-α cytokines in the mice immunized with p VAX1-F1 L+p VAX1-IL-2 were significantly higher than those in the p VAX1-F1 L group,compared with the p VAX1 group and the PBS group.The difference between the groups was extremely significant(P<0.01).The levels of serum IL-4,IL-6,and IL-10 cytokines in the p VAX1-F1 L+p VAX1-IL-2 combined group were significantly higher than those in the p VAX1 group and the PBS group.Compared with the p VAX1-F1 L immunized group,the difference was not significant(P>0.05);and the p VAX1-F1 L+p VAX1-IL-2 immunized group had higher levels of spleen lymphocyte proliferation than the p VAX1-F1 L immunized group,the p VAX1 group and the PBS group(P<0.01).The mice immunized with p VAX1-IL-2 and p VAX1-F1 L could induce Orf V-specific humoral and cellular immunity.The immune responses induced by p VAX1-IL-2 were mainly cellular immune responses,which promoted the secretion of Th1 cytokines.The above-mentioned results provide the basis for further development of Orf V nucleic acid vaccines.
引文
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[15]王洪光,郝飞,汤德元,等.伪狂犬病毒gE基因疫苗在动物体内诱导的免疫应答及IL-2对其作用影响的研究[J].畜牧兽医学报,2014,45(9):1488-1496.WANG Hong-guang,HAO Fei,TANG De-yuan,et al.Immune responses of pseudorabies virus gE DNA vaccine in animals and the immune effects of interleuki-2 gene on it[J].Chinese Journal of Animal and Veterinary Sciences,2014,45(9):1488-1496.(in Chinese)
[16]FERNáNDEZ-RUIZ M,HUMAR A1,BALUCH A2,et al.Baseline serum interleukin-6 to interleukin-2 ratio is associated with the response to seasonal trivalent influenza vaccine in solid organ transplant recipients[J].Vaccine,2015,33(51):7176-7182.
[17]李世芳,刘泽众,常惠芸.细胞因子佐剂研究进展[J].中国畜牧兽医,2017,44(1):296-301.LI Shi-fang,LIU Ze-zhong,CHANG Hui-yun.Research progress on cytokine adjuvants[J].China Animal Husbandry&Veterinary Medicine,2017,44(1):296-301.(in Chinese)
[2]鲜思美,胡廷帅,熊朝丽,等.贵州省羊口疮的调查研究[J].动物医学进展,2012,33(11):73-76.XIAN Si-mei,HU Ting-shuai,XIONG Chao-li,et al.Investigation on Orf in Guizhou Province[J].Progress in Veterinary Medicine,2012,33(11):73-76.(in Chinese)
[3]田婷婷,李杰,姚运亮,等.羊口疮病毒灭活疫苗的制备及免疫效果观察[J].动物医学进展,2013,34(6):17-20.TIAN Ting-ting,LI Jie,YAO Yun-liang,et al.Preparation of Orf virus inactivated vaccine and assessment of immune effect[J].Progress in Veterinary Medicine,2013,34(6):17-20.(in Chinese)
[4]张蕾,汪嘉,方小楠,等.核酸疫苗的作用及我国目前的研究现状[J].放射免疫学杂志,2013,26(4):432-436.ZHANG Lei,WANG Jia,FANG Xiao-nan,et al.The role of nucleic acid vaccine and the present research status in China[J].Journal of Radioimmunology,2013,26(4):432-436.(in Chinese)
[5]闫丰超,邵佳,窦永喜.羊口疮病毒分子生物学的研究进展[J].中国兽医科学,2013,43(1):103-109.YAN Feng-chao,SHAO Jia,DOU Yong-xi.Progress in molecular biology of orf virus[J].Chinese Veterinary Science,2013,43(1):103-109.(in Chinese)
[6]刘嫒,杨钰,鲜思美,等.羊口疮病毒贵州株F1L基因克隆及生物信息学分析[J].中国畜牧兽医,2015,42(1):53-60.LIU Ai,YNAG Yu,XIAN Si-mei,et al.Cloning and bioinformatic analysis of F1L gene of Orf virus from Guizhou Province[J].China Animal Husbandry&Veterinary Medicine,2015,42(1):53-60.(in Chinese)
[7]赵定亮,单风平.白细胞介素-2最新研究进展[J].微生物学杂志,2013,33(4):77-83.ZHAO Ding-liang,SHAN Feng-ping.The latest research progress of interleukin-2[J].Journal of Microbiology,2013,33(4):77-83.(in Chinese)
[8]ZHANG H X,QIU Y Y,ZHAO Y H,et al.Immunogenicity of oral vaccination with Lactococcus lactis derived vaccine candidate antigen(UreB)of Helicobacter pylori fused with the human interleukin 2 as adjuvant[J].Molecular and Cellular Probes,2014,28(1):25-30.
[9]PASSALACQUA R,CAMINITI C,BUTI S,et al.Adjuvant low-dose interleukin-2(IL-2)plus interferon-α(IFN-α)in operable renal cell carcinoma(RCC):a phaseⅢ,randomized,multicentre trial of the Italian Oncology Group for Clinical Research(GOIRC)[J].Journal of Immunotherapy,2014,37(9):440-447.
[10]刘嫒,冯将,鲜思美,等.羊口疮病毒F1L和B2L基因真核表达质粒构建及其在MDBK细胞中的表达[J].中国人兽共患病学报,2015,31(12):1124-1128.LIU Ai,FENG Jiang,XIAN Si-mei,et al.Construction and expression of recombinant plasmids of OrfV F1L and B2L genes in MDBK cells[J].Chinese Journal of Zoonoses,2015,31(12):1124-1128.(in Chinese)
[11]SCAGLIARINI A,CIULLI S,BATTILANI M,et al.Characterisation of immunodominant protein encoded by the F1L gene of orf virus strains isolated in Italy[J].Archives of Virology,2002,147(10):1989-1995.
[12]GALLINA L,SCAGLIARINL A,CIULII S,et al.Prosperi cloning and express ion of the Orf virus F1L gene,possible use as a subunit vaccine[J].Veterinary Research Communications,2004,28(1):291-293.
[13]ZHAO K,HE W,GAO W,et al.Orf virus DNA vaccines expressing ORFV 011 and ORFV 059 chimeric protein enhances immunogenicity[J].Virology Journal,2011,8(1):562.
[14]丁忠庆,沈荣显,相文华,等.IL-2和绵羊梅迪-维斯纳病病毒核心蛋白Gag核酸疫苗联合免疫小鼠的免疫应答[J].中国预防兽医学报,2007,29(3):204-207.DING Zhong-qing,SHEN Rong-xian,XIANG Wen-hua,et al.The immune response in mice inoculated with maedi-visna virus Gag gene DNA vaccine and IL-2plasmid[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(3):204-207.(in Chinese)
[15]王洪光,郝飞,汤德元,等.伪狂犬病毒gE基因疫苗在动物体内诱导的免疫应答及IL-2对其作用影响的研究[J].畜牧兽医学报,2014,45(9):1488-1496.WANG Hong-guang,HAO Fei,TANG De-yuan,et al.Immune responses of pseudorabies virus gE DNA vaccine in animals and the immune effects of interleuki-2 gene on it[J].Chinese Journal of Animal and Veterinary Sciences,2014,45(9):1488-1496.(in Chinese)
[16]FERNáNDEZ-RUIZ M,HUMAR A1,BALUCH A2,et al.Baseline serum interleukin-6 to interleukin-2 ratio is associated with the response to seasonal trivalent influenza vaccine in solid organ transplant recipients[J].Vaccine,2015,33(51):7176-7182.
[17]李世芳,刘泽众,常惠芸.细胞因子佐剂研究进展[J].中国畜牧兽医,2017,44(1):296-301.LI Shi-fang,LIU Ze-zhong,CHANG Hui-yun.Research progress on cytokine adjuvants[J].China Animal Husbandry&Veterinary Medicine,2017,44(1):296-301.(in Chinese)