α-萘乙酸甲酯对马铃薯贮藏期营养品质的影响及残留动态研究
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摘要
为研究贮藏期间α-萘乙酸甲酯(MENA)对马铃薯品质营养功能的影响及其残留动态变化,以"山东潍坊菜用马铃薯"为试材,分别用质量浓度40、80 mg/kg的MENA溶液进行喷洒处理,以清水处理作为对照,置于4℃条件下贮藏,定期(30 d)检测马铃薯的腐烂率、发芽率、干物质、淀粉、VC、还原糖、蛋白质、绿原酸含量及MENA残留量等品质指标。结果表明,与对照相比MENA能减低贮藏期马铃薯的腐烂率和发芽率,有利于马铃薯外观形态的保持;MENA能延缓马铃薯贮藏期间VC含量的降低(p<0.05)且使蛋白质含量升高(p<0.05),能使还原糖含量显著升高(p<0.05)并提升绿原酸水平(p<0.05);贮藏期间MENA残留未检出,提示MENA作为抑芽剂使用其残留风险较低。
Aiming to evaluate the effects of MENA on the quality nutrition function of potato tubers and its residual dynamics during storage,vegetable processing potato tubers from Weifang city in Shandong province were taken as the test material. The sprout inhibitor α-naphthyl acetate( MENA) was applied to potato tubers by a direct spray application at 40 and80 mg/kg for two treatment groups while the spraying water was for the control group.These groups were storaged at 4 ℃ for 150 days.Decay rate,sprout rate,reducing sugar,starch,dry matter,protein,vitamin C and chlorogenic acid content of potato tubers were detected every 30 days.The results showed that MENA could reduce potato tubers decay rate and sprout rate during storage,and could maintain the appearance of potato tubers compared with the control group.MENA could delay the reduction of VCcontent( p < 0.05) and raise protein content( p < 0.05) during potato tubers storage,it also could significantly increase the content of reducing sugar( p < 0.05) and raise the level of chlorogenic acid( p < 0.05). Residue concentrations of MENA on potato tubers were not detected during storage,suggesting that MENA had a lower risk of residue as a sprout inhibitor.
Aiming to evaluate the effects of MENA on the quality nutrition function of potato tubers and its residual dynamics during storage,vegetable processing potato tubers from Weifang city in Shandong province were taken as the test material. The sprout inhibitor α-naphthyl acetate( MENA) was applied to potato tubers by a direct spray application at 40 and80 mg/kg for two treatment groups while the spraying water was for the control group.These groups were storaged at 4 ℃ for 150 days.Decay rate,sprout rate,reducing sugar,starch,dry matter,protein,vitamin C and chlorogenic acid content of potato tubers were detected every 30 days.The results showed that MENA could reduce potato tubers decay rate and sprout rate during storage,and could maintain the appearance of potato tubers compared with the control group.MENA could delay the reduction of VCcontent( p < 0.05) and raise protein content( p < 0.05) during potato tubers storage,it also could significantly increase the content of reducing sugar( p < 0.05) and raise the level of chlorogenic acid( p < 0.05). Residue concentrations of MENA on potato tubers were not detected during storage,suggesting that MENA had a lower risk of residue as a sprout inhibitor.
引文
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[15]刁小琴,关海宁.响应面优化超声辅助提取马铃薯皮中绿原酸工艺[J].食品工业,2014(9):8-12.
[16]陈连才,王殿友,李萍,等.采用气相色谱法测定α-萘乙酸含量[J].高师理科学刊,2000(3):46-48.
[17]纳添仓,阮建平,唐小兰,等.马铃薯贮藏的方式与技术[J].青海农林科技,2002(3):34-35.
[18]孙一峰,黄文榜.α—萘乙酸及其甲酯的合成及应用研究[J].广西化工,1997(2):34-39.
[19]吴晓玲,任晓月,陈彦云,等.贮藏温度对马铃薯营养物质含量及酶活性的影响[J].江苏农业科学,2012,40(5):220-222.
[20]冯利波,吴林科,买买提.托合提苏莱曼.贮藏期间马铃薯几个主要加工品质指标的变化[J].中国马铃薯,2006(2):88-90.
[21]朱旭.贮藏温度和堆码高度对克新1号马铃薯贮藏损失及品质影响的研究[D].吉林:吉林大学,2014.
[22]Claassen P A M,Budde M A W,Van Calker M H.Increase in phosphorylase activity during cold-induced sugar accumulation in potato tubers[J].Potato Research,1993,36(3):205-217.
[23]陈彦云.马铃薯贮藏期间干物质、还原糖、淀粉含量的变化[J].中国农学通报,2006(4):84-87.
[24]巩慧玲,赵萍,杨俊丰.马铃薯贮藏期间淀粉和还原糖含量的变化及回温处理的影响[J].食品工业科技,2008(2):277-279.
[25]吴婷.壳聚糖处理对采后马铃薯块茎发芽及品质影响[D].杭州:浙江工商大学,2016.
[26]Dale M F B,Griffiths D W,Todd D T.Effects of genotype,environment,and postharvest storage on the total ascorbate content of potato(Solanum tuberosum)tubers[J].Journal of Agricultural and Food Chemistry,2003,51(1):244-248.
[27]范芳,刘家伟.α-萘乙酸甲酯对土豆发芽生长影响[J].广东石油化工学院学报,2013,23(6):27-30.
[28]刘杰.低温胁迫下柑桔生理生化变化及差异蛋白质的表达[D].湖南:湖南农业大学,2006.
[29]Lewis C E,Walker J R L,Lancaster J E,et al.Determination of anthocyanins,flavonoids and phenolic acids in potatoes.I:Coloured cultivars of Solanum tuberosum L[J].Journal of the Science of Food and Agriculture,1998,77(1):45-57.
[30]徐晓梅,杨署光.苯丙氨酸解氨酶研究进展[J].安徽农业科学,2009,37(31):15115-15119,15122.
[31]王礼群,刘硕,杨春贤,等.鲜切甘薯不同部位褐变机理差异研究[J].食品科学,:1-8.
[2]张路.氯苯胺灵对马铃薯贮藏作用效果及残留研究[D].内蒙古:内蒙古农业大学,2011.
[3]程建新.环境温度对贮藏初期马铃薯块茎生理的影响[A].中国作物学会马铃薯专业委员会、河北省农业厅、张家口市人民政府.2016年中国马铃薯大会论文集[C].中国作物学会马铃薯专业委员会、河北省农业厅、张家口市人民政府:,2016:5.
[4]Sonnewald U.Control of potato tuber sprouting.[J].Trends in Plant Science,2001,6(8):33-35.
[5]李守强,田世龙,颉敏华,等.氯苯胺灵在马铃薯中残留及降解动态的研究[J].保鲜与加工,2009,9(2):35-37.
[6]陈彦云,刘成敏,郑学平,等.马铃薯抑芽剂研制效果实验[J].中国马铃薯,2001(5):284-285.
[7]Danielslake B J,Prange R K,Nowak J,et al.Sprout development and processing quality changes in potato tubers stored under ethylene:1.Effects of ethylene concentration[J].American Journal of Potato Research,2005,82(5):389-397.
[8]李彦杰,杨俊年,刘仁华,等.芽期和发酵期马铃薯中龙葵素含量动态及组分分析[J].黑龙江畜牧兽医,2014(13):114-116.
[9]阚琳玮.不同处理方式对马铃薯品质的影响研究[D].郑州:河南工业大学,2014.
[10]于延申.抑芽剂和防腐剂在马铃薯贮藏上的应用[J].蔬菜科技,2015(7):46-47.梅文泉,隋启君,佴注,等.马铃薯块茎中还原糖测定的一种方法[J].云南农业科技,2003(3):23-24.
[11]Yada R Y,Coffin R H,Keenan M K,et al.The effect of maleic hydrazide(potassium salt)on potato yield,sugar content and chip color of Kennebec and Norchip cultivars[J].American Potato Journal,1991,68(10):705-709.
[12]葛霞.马铃薯抑芽剂的初选研究[A].中国作物学会马铃薯专业委员会、河北省农业厅、张家口市人民政府.2016年中国马铃薯大会论文集[C].中国作物学会马铃薯专业委员会、河北省农业厅、张家口市人民政府:,2016:7.
[13]裴明黎.植物生长调节剂MENA和MH对马铃薯采后贮藏品质的影响及安全性评价[D].杭州:浙江大学,2010.
[14]梅文泉,隋启君,佴注,等.马铃薯块茎中还原糖测定的一种方法[J].云南农业科技,2003(3):23-24.
[15]刁小琴,关海宁.响应面优化超声辅助提取马铃薯皮中绿原酸工艺[J].食品工业,2014(9):8-12.
[16]陈连才,王殿友,李萍,等.采用气相色谱法测定α-萘乙酸含量[J].高师理科学刊,2000(3):46-48.
[17]纳添仓,阮建平,唐小兰,等.马铃薯贮藏的方式与技术[J].青海农林科技,2002(3):34-35.
[18]孙一峰,黄文榜.α—萘乙酸及其甲酯的合成及应用研究[J].广西化工,1997(2):34-39.
[19]吴晓玲,任晓月,陈彦云,等.贮藏温度对马铃薯营养物质含量及酶活性的影响[J].江苏农业科学,2012,40(5):220-222.
[20]冯利波,吴林科,买买提.托合提苏莱曼.贮藏期间马铃薯几个主要加工品质指标的变化[J].中国马铃薯,2006(2):88-90.
[21]朱旭.贮藏温度和堆码高度对克新1号马铃薯贮藏损失及品质影响的研究[D].吉林:吉林大学,2014.
[22]Claassen P A M,Budde M A W,Van Calker M H.Increase in phosphorylase activity during cold-induced sugar accumulation in potato tubers[J].Potato Research,1993,36(3):205-217.
[23]陈彦云.马铃薯贮藏期间干物质、还原糖、淀粉含量的变化[J].中国农学通报,2006(4):84-87.
[24]巩慧玲,赵萍,杨俊丰.马铃薯贮藏期间淀粉和还原糖含量的变化及回温处理的影响[J].食品工业科技,2008(2):277-279.
[25]吴婷.壳聚糖处理对采后马铃薯块茎发芽及品质影响[D].杭州:浙江工商大学,2016.
[26]Dale M F B,Griffiths D W,Todd D T.Effects of genotype,environment,and postharvest storage on the total ascorbate content of potato(Solanum tuberosum)tubers[J].Journal of Agricultural and Food Chemistry,2003,51(1):244-248.
[27]范芳,刘家伟.α-萘乙酸甲酯对土豆发芽生长影响[J].广东石油化工学院学报,2013,23(6):27-30.
[28]刘杰.低温胁迫下柑桔生理生化变化及差异蛋白质的表达[D].湖南:湖南农业大学,2006.
[29]Lewis C E,Walker J R L,Lancaster J E,et al.Determination of anthocyanins,flavonoids and phenolic acids in potatoes.I:Coloured cultivars of Solanum tuberosum L[J].Journal of the Science of Food and Agriculture,1998,77(1):45-57.
[30]徐晓梅,杨署光.苯丙氨酸解氨酶研究进展[J].安徽农业科学,2009,37(31):15115-15119,15122.
[31]王礼群,刘硕,杨春贤,等.鲜切甘薯不同部位褐变机理差异研究[J].食品科学,:1-8.