壳寡糖通便功能的研究
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摘要
研究壳寡糖对小鼠通便功能。将昆明雄性小鼠按体重随机分成空白对照组、模型对照组和3个剂量组。剂量组分别经口给予低、中、高剂量(99.2、198.4、396.8 mg/(kg·d))壳寡糖溶液;空白对照组和模型对照组给予蒸馏水,干预时间为7 d。用盐酸洛哌丁胺造小鼠便秘模型。观察各组小鼠的小肠墨汁推进率、首粒排黑便时间、6 h黑便粒数和6 h黑便重量以及粪便含水量。结果表明:与模型对照组比,壳寡糖中剂量组小肠墨汁推进率和6 h黑便重量显著增加(p<0.05);壳寡糖高剂量组小肠墨汁推进率极显著增加(p<0.01)、首粒排黑便时间极显著缩短(p<0.01)、6 h黑便重量与6 h黑便粒数显著增加(p<0.05);在粪便含水量上,各剂量组同空白对照组、模型对照组比无显著性差异。壳寡糖能有效增强小鼠肠道蠕动并促进排便,具有通便功能。
To investigate the laxative effect of chitooligosaccharide in mice. Kunming male mice were randomly divided into blank control group,model control group and three dose groups. Dose groups were given low,medium and high doses of chitooligosaccharide solution [99.2,198.4,396.8 mg/(kg·d)],while the blank control group and model control group were given distilled water,with 7 day intervention. Mouse model was made with loperamide hydrochloride. Ink propulsion rate in the small intestine,the time of defecation of the first black feces,the defecation amount and weights in six hours,and the moisture content of stool were observed in each group. Results showed that:compared with the model control group,the medium dose of chitooligosaccharide could increase intestinal propulsive rate and the dejection weights in six hours significantly(p<0.05);the high dose of chitooligosaccharide could significantly increase intestinal propulsive rate(p <0.01),the dejection amount and weights in six hours(p<0.05),and significantly shorten the time of defecation of the first black feces(p<0.01). There was no significant difference between the dose group and the blank control group and model control group in the water content of feces. The chitooligosaccharide significantly improves mice's defecation and intestinal peristalsis with laxative effect.
To investigate the laxative effect of chitooligosaccharide in mice. Kunming male mice were randomly divided into blank control group,model control group and three dose groups. Dose groups were given low,medium and high doses of chitooligosaccharide solution [99.2,198.4,396.8 mg/(kg·d)],while the blank control group and model control group were given distilled water,with 7 day intervention. Mouse model was made with loperamide hydrochloride. Ink propulsion rate in the small intestine,the time of defecation of the first black feces,the defecation amount and weights in six hours,and the moisture content of stool were observed in each group. Results showed that:compared with the model control group,the medium dose of chitooligosaccharide could increase intestinal propulsive rate and the dejection weights in six hours significantly(p<0.05);the high dose of chitooligosaccharide could significantly increase intestinal propulsive rate(p <0.01),the dejection amount and weights in six hours(p<0.05),and significantly shorten the time of defecation of the first black feces(p<0.01). There was no significant difference between the dose group and the blank control group and model control group in the water content of feces. The chitooligosaccharide significantly improves mice's defecation and intestinal peristalsis with laxative effect.
引文
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[6]Jemal A,Siegel R,Xu J,et al.Cancer statistics,2010[J].Ca A Cancer Journal for Clinicians,2010,60(5):277-300
[7]Ghadirian P,Maisonneuve P,Perret C,et al.Epidemiology of sociodemographic characteristics,lifestyle,medical history,and colon cancer:a case-control study among French Canadians in Montreal[J].Cancer Detection&Prevention,1998,22(5):396-404
[8]Belsey J,Greenfield S,Candy D,et al.Systematic review:impact of constipation on quality of life in adults and children[J].Alimentary Pharmacology&Therapeutics,2010,31(9):938-949
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[10]杨怡.饮食干预对功能性便秘疗效维持的影响[D].北京:北京中医药大学,2016
[11]Buddington R K,Kapadia C,Neumer F,et al.Oligofructose Provides Laxation for Irregularity Associated with Low Fiber Intake.[J].Nutrients,2017,1372(9):1-11
[12]Wang L,Pan M,Li D,et al.Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice[J].Journal of Functional Foods,2017,38:486-496
[13]程明明,黄苇.西番莲果皮中膳食纤维的降脂保肝及润肠通便功能[J].食品科学,2017,38(11):202-207
[14]Rao S S,Yu S,Fedewa A.Systematic review:dietary fibre and FODMAP-restricted diet in the management of constipation and irritable bowel syndrome[J].Aliment Pharmacol Ther,2015,41(12):1256-1270
[15]Chmielewska A,Szajewska H.Systematic review of randomised controlled trials:Probiotics for functional constipation[J].World Journal of Gastroenterology,2010,16(1):69-75
[16]Chassagne P,Ducrotte P,Garnier P,et al.Tolerance and Long-Term Efficacy of Polyethylene Glycol 4000(ForlaxR)Compared to Lactulose in Elderly Patients with Chronic Constipation[J].Journal of Nutrition Health&Aging,2017,21(4):429-439
[17]Dipalma J A,Cleveland M V,Mcgowan J,et al.A Randomized,Multicenter,Placebo-Controlled Trial of Polyethylene Glycol Laxative for Chronic Treatment of Chronic Constipation[J].American Journal of Gastroenterology,2007,102(7):1436-1441
[18]沃联群,方琳,杨明华,等.番泻叶不同煎煮时间的水提物对小鼠便秘模型的影响[J].中药新药与临床药理,2013,24(5):449-451
[19]张春玲.芦荟预防与治疗慢传输型便秘的作用及机制研究[D].济南:山东大学,2008
[20]Barish C F,Drossman D,Johanson J F,et al.Efficacy and safety of lubiprostone in patients with chronic constipation[J].Digestive Diseases&Sciences,2010,55(4):1090-1097
[21]Grider J R,Foxxorenstein A E,Jin J G.5-Hydroxytryptamine4 receptor agonists initiate the peristaltic reflex in human,rat,and guinea pig intestine.[J].Gastroenterology,1998,115(2):370-380
[22]彭皓,赵芳芳,张艳芳,等.糖工程与壳寡糖的概述及其应用研究进展[J].饲料与畜牧:新饲料,2012(11):14-18
[23]中华人民共和国卫生部.卫监发[2003]42号保健食品检验与评价技术规范[S].北京:人民卫生出版社,2003:153-161
[24]Zhu F,Xu S,Zhang Y,et al.Total Glucosides of Paeony Promote Intestinal Motility in Slow Transit Constipation Rats through Amelioration of Interstitial Cells of Cajal:[J].Plos One,2016,11(8):1-10
[25]褚慰,付小林,樊智慧,等.低聚果糖对便秘模型小鼠的通便功能的研究[J].中国药物与临床,2010,10(10):1135-1136
[26]张俊黎,王宇,于红霞,等.低聚果糖对动物通便功能研究[J].预防医学情报杂志,2007,23(5):534-536
[27]王鑫,朱婧,刘静,等.低聚木糖润肠通便及调节肠道菌群功能的研究[J].食品工业科技,2015,36(14):359-362
[28]汪雨亭,陈挺强,王娟.香蕉低聚糖的通便功能评价[J].食品工业科技,2017,38(3):357-364
[29]Mendlik K,Albrecht J A,Schnepf M.Effects of Fructooligofructoses Chain Length on the Bifidobacteria of the Human Colon:A Pilot Study[J].Food&Nutrition Sciences,2012,3(12):1615-1618
[30]Han S H,Hong K B,Kim E Y,et al.Effect of dual-type oligosaccharides on constipation in loperamide-treated rats[J].Nutrition Research&Practice,2016,10(6):583-589
[31]Kwon J I,Park Y,Dong O N,et al.Complex-oligosaccharide composed of galacto-oligosaccharide and lactulose ameliorates loperamide-induced constipation in rats[J/OL].(2018-01-05)[2018-03-06].https://link.springer.com/article/10.1007/s10068-017-0300-2
[32]Wang L,Hu L,Yan S,et al.Effects of different oligosaccharides at various dosages on the composition of gut microbiota and shortchain fatty acids in mice with constipation[J].Food&Function,2017,8(5):1966-1978
[33]魏涛,赵建元,高兆兰,等.低聚木糖调节BALB/c小鼠肠道菌群及润肠通便作用研究[J].安徽农业科学,2013,41(1):159-160
[34]Wang L,Pan M,Li D,et al.Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice[J].Journal of Functional Foods,2017,38:486-496
[35]Li T,Lu X,Yang X.Evaluation of clinical safety and beneficial effects of stachyose-enrichedα-galacto-oligosaccharides on gut microbiota and bowel function in humans[J].Food&Function,2017,8(1):262-269
[36]Eswaran S,Muir J,Chey W D.Fiber and functional gastrointestinal disorders.[J].American Journal of Gastroenterology,2013,108(5):718-727
[2]郭晓峰,柯美云,潘国宗,等.北京地区成人慢性便秘整群、分层、随机流行病学调查及其相关因素分析[J].中华消化杂志,2002,22(10):637-638
[3]熊理守,陈旻湖,陈惠新,等.广东省社区人群慢性便秘的流行病学研究[J].中华消化杂志,2004,24(8):488-491
[4]刘智勇,杨关根,沈忠,等.杭州市城区便秘流行病学调查[J].中华消化杂志,2004,24(7)435-436
[5]申杰,叶希韵,沈菊,等.壳寡糖对高脂血症小鼠降血脂及肝脏保护的作用[J].西北农林科技大学学报(自然科学版),2007,35(9):35-38
[6]Jemal A,Siegel R,Xu J,et al.Cancer statistics,2010[J].Ca A Cancer Journal for Clinicians,2010,60(5):277-300
[7]Ghadirian P,Maisonneuve P,Perret C,et al.Epidemiology of sociodemographic characteristics,lifestyle,medical history,and colon cancer:a case-control study among French Canadians in Montreal[J].Cancer Detection&Prevention,1998,22(5):396-404
[8]Belsey J,Greenfield S,Candy D,et al.Systematic review:impact of constipation on quality of life in adults and children[J].Alimentary Pharmacology&Therapeutics,2010,31(9):938-949
[9]Martin B C,Barghout V,Cerulli A.Direct medical costs of constipation in the United States.[J].Managed Care Interface,2006,19(12):43-49
[10]杨怡.饮食干预对功能性便秘疗效维持的影响[D].北京:北京中医药大学,2016
[11]Buddington R K,Kapadia C,Neumer F,et al.Oligofructose Provides Laxation for Irregularity Associated with Low Fiber Intake.[J].Nutrients,2017,1372(9):1-11
[12]Wang L,Pan M,Li D,et al.Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice[J].Journal of Functional Foods,2017,38:486-496
[13]程明明,黄苇.西番莲果皮中膳食纤维的降脂保肝及润肠通便功能[J].食品科学,2017,38(11):202-207
[14]Rao S S,Yu S,Fedewa A.Systematic review:dietary fibre and FODMAP-restricted diet in the management of constipation and irritable bowel syndrome[J].Aliment Pharmacol Ther,2015,41(12):1256-1270
[15]Chmielewska A,Szajewska H.Systematic review of randomised controlled trials:Probiotics for functional constipation[J].World Journal of Gastroenterology,2010,16(1):69-75
[16]Chassagne P,Ducrotte P,Garnier P,et al.Tolerance and Long-Term Efficacy of Polyethylene Glycol 4000(ForlaxR)Compared to Lactulose in Elderly Patients with Chronic Constipation[J].Journal of Nutrition Health&Aging,2017,21(4):429-439
[17]Dipalma J A,Cleveland M V,Mcgowan J,et al.A Randomized,Multicenter,Placebo-Controlled Trial of Polyethylene Glycol Laxative for Chronic Treatment of Chronic Constipation[J].American Journal of Gastroenterology,2007,102(7):1436-1441
[18]沃联群,方琳,杨明华,等.番泻叶不同煎煮时间的水提物对小鼠便秘模型的影响[J].中药新药与临床药理,2013,24(5):449-451
[19]张春玲.芦荟预防与治疗慢传输型便秘的作用及机制研究[D].济南:山东大学,2008
[20]Barish C F,Drossman D,Johanson J F,et al.Efficacy and safety of lubiprostone in patients with chronic constipation[J].Digestive Diseases&Sciences,2010,55(4):1090-1097
[21]Grider J R,Foxxorenstein A E,Jin J G.5-Hydroxytryptamine4 receptor agonists initiate the peristaltic reflex in human,rat,and guinea pig intestine.[J].Gastroenterology,1998,115(2):370-380
[22]彭皓,赵芳芳,张艳芳,等.糖工程与壳寡糖的概述及其应用研究进展[J].饲料与畜牧:新饲料,2012(11):14-18
[23]中华人民共和国卫生部.卫监发[2003]42号保健食品检验与评价技术规范[S].北京:人民卫生出版社,2003:153-161
[24]Zhu F,Xu S,Zhang Y,et al.Total Glucosides of Paeony Promote Intestinal Motility in Slow Transit Constipation Rats through Amelioration of Interstitial Cells of Cajal:[J].Plos One,2016,11(8):1-10
[25]褚慰,付小林,樊智慧,等.低聚果糖对便秘模型小鼠的通便功能的研究[J].中国药物与临床,2010,10(10):1135-1136
[26]张俊黎,王宇,于红霞,等.低聚果糖对动物通便功能研究[J].预防医学情报杂志,2007,23(5):534-536
[27]王鑫,朱婧,刘静,等.低聚木糖润肠通便及调节肠道菌群功能的研究[J].食品工业科技,2015,36(14):359-362
[28]汪雨亭,陈挺强,王娟.香蕉低聚糖的通便功能评价[J].食品工业科技,2017,38(3):357-364
[29]Mendlik K,Albrecht J A,Schnepf M.Effects of Fructooligofructoses Chain Length on the Bifidobacteria of the Human Colon:A Pilot Study[J].Food&Nutrition Sciences,2012,3(12):1615-1618
[30]Han S H,Hong K B,Kim E Y,et al.Effect of dual-type oligosaccharides on constipation in loperamide-treated rats[J].Nutrition Research&Practice,2016,10(6):583-589
[31]Kwon J I,Park Y,Dong O N,et al.Complex-oligosaccharide composed of galacto-oligosaccharide and lactulose ameliorates loperamide-induced constipation in rats[J/OL].(2018-01-05)[2018-03-06].https://link.springer.com/article/10.1007/s10068-017-0300-2
[32]Wang L,Hu L,Yan S,et al.Effects of different oligosaccharides at various dosages on the composition of gut microbiota and shortchain fatty acids in mice with constipation[J].Food&Function,2017,8(5):1966-1978
[33]魏涛,赵建元,高兆兰,等.低聚木糖调节BALB/c小鼠肠道菌群及润肠通便作用研究[J].安徽农业科学,2013,41(1):159-160
[34]Wang L,Pan M,Li D,et al.Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice[J].Journal of Functional Foods,2017,38:486-496
[35]Li T,Lu X,Yang X.Evaluation of clinical safety and beneficial effects of stachyose-enrichedα-galacto-oligosaccharides on gut microbiota and bowel function in humans[J].Food&Function,2017,8(1):262-269
[36]Eswaran S,Muir J,Chey W D.Fiber and functional gastrointestinal disorders.[J].American Journal of Gastroenterology,2013,108(5):718-727