硒对邻苯二甲酸酯亚慢性暴露雄性大鼠肝脏的保护作用
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摘要
目的:研究邻苯二甲酸酯(DEHP)亚慢性暴露对雄性大鼠肝脏的影响及硒对大鼠肝脏的保护作用。方法:将77只雄性大鼠随机分为7组,分别设置为对照组(饲喂基础饲料)、3个DEHP染毒组(染毒剂量分别为300、600和900mg/kg)、3个硒干预组(在相应剂量的染毒组中加入1mg/kg酵母硒)。染毒8周,染毒期末,大鼠空腹16h后称量大鼠体质量、采血进行生化检测以及称量肝脏质量。结果:与对照组相比,600和900mg/kgDEHP染毒组大鼠的平均体质量明显降低(P<0.05);900mg/kgDEHP染毒组大鼠的体质量增量、总摄食量、总食物利用率、明显降低(P<0.01或P<0.05);各DEHP染毒组、硒干预组的肝脏平均质量、肝脏系数均明显降低(P<0.01)。600mg/kgDEHP染毒后的硒干预组大鼠体质量增量较相应的染毒组增加(P<0.05)。生化指标检测结果显示,与对照组相比,600、900mg/kgDEHP染毒组,300、600mg/kgDEHP染毒后的硒干预组大鼠ALT浓度明显升高(P<0.05);900mg/kgDEHP染毒组,300、600和900mg/kgDEHP染毒后的硒干预组大鼠AST浓度明显升高(P<0.01);600、900mg/kgDEHP染毒组大鼠TP浓度明显升高(P<0.01);900mg/kgDEHP染毒组,300mg/kgDEHP染毒后的硒干预组大鼠ALB浓度明显升高(P<0.01);900mg/kgDEHP染毒组大鼠GLB浓度明显升高(P<0.01)。900mg/kgDEHP染毒后的硒干预组大鼠ALT、AST、TP、GLB均明显低于相应的染毒组(P<0.01或P<0.05)。结论:DEHP的亚慢性暴露对雄性大鼠的体质量与生长产生一定的影响,可能造成肝脏肿胀,对肝脏功能产生影响;而硒的干预可能在一定程度上缓解DEHP对肝脏的毒性作用。
OBJECTIVE: To investigate effects of sub-chronic exposure to diethylhexyl phthalate(DEHP) on livers of male rats and protective effect of selenium on the livers. METHODS:Seventy-seven male rats were randomly divided into 7 groups:control group(feeding basal feed),3 DEHP-treatment groups(diluted doses of 300,600 and 900 mg/kg,respectively),and 3 Selenium intervention groups(1 mg/kg yeast selenium was added to the corresponding dose of the exposure group). After 8 weeks of exposure,the rats were weighed,blood samples were collected for biochemical detection and the excised liver was weighed after 16 hours fasting. RESULTS:Compared with the control group,the mean weight of livers and coefficient of the liver were significantly increased in each of the exposed groups and the intervention group(P<0.01). In addition,the quality of the 600 mg/kg treated group was significantly reduced(P<0.05);the 900 mg/kg treated group was significantly lower in the body weight gain index(P<0.01),and significantly lower than the control group in the total food intake and utilization index(P<0.05);Compared with the treated group,the Se+300 mg/kg intervention group showed a significant decrease in weight gain(P<0.05). The results of biochemical indicators showed that,compared with the control group,the 600 mg/kg treated group showed a significant increase in TP(P<0.01);the 900 mg/kg treated group showed a significant increase in all five indicators(P<0.01);The Se+300 mg/kg intervention group significantly increased the ALT index(P<0.01),and significantly increased the ALT and AST indexes(P<0.01). The Se+600 mg/kg intervention group significantly increased the ALT index(P<0.05). There was a significant increase in AST index(P<0.01). The Se+900 mg/kg intervention group showed significantly increased AST,TP and GLB(P<0.01). Compared with the treated group,the intervention group was lower than the corresponding exposure group,and the Se+900 mg/kg intervention group showed a significant decrease in TP and GLB indicators(P<0.01). CONCLUSION:Sub-chronic exposure of DEHP affected body weight and growth,and liver function as well as caused liver swelling. The intervention of selenium effectively alleviated the toxic effect of DEHP on the liver.
OBJECTIVE: To investigate effects of sub-chronic exposure to diethylhexyl phthalate(DEHP) on livers of male rats and protective effect of selenium on the livers. METHODS:Seventy-seven male rats were randomly divided into 7 groups:control group(feeding basal feed),3 DEHP-treatment groups(diluted doses of 300,600 and 900 mg/kg,respectively),and 3 Selenium intervention groups(1 mg/kg yeast selenium was added to the corresponding dose of the exposure group). After 8 weeks of exposure,the rats were weighed,blood samples were collected for biochemical detection and the excised liver was weighed after 16 hours fasting. RESULTS:Compared with the control group,the mean weight of livers and coefficient of the liver were significantly increased in each of the exposed groups and the intervention group(P<0.01). In addition,the quality of the 600 mg/kg treated group was significantly reduced(P<0.05);the 900 mg/kg treated group was significantly lower in the body weight gain index(P<0.01),and significantly lower than the control group in the total food intake and utilization index(P<0.05);Compared with the treated group,the Se+300 mg/kg intervention group showed a significant decrease in weight gain(P<0.05). The results of biochemical indicators showed that,compared with the control group,the 600 mg/kg treated group showed a significant increase in TP(P<0.01);the 900 mg/kg treated group showed a significant increase in all five indicators(P<0.01);The Se+300 mg/kg intervention group significantly increased the ALT index(P<0.01),and significantly increased the ALT and AST indexes(P<0.01). The Se+600 mg/kg intervention group significantly increased the ALT index(P<0.05). There was a significant increase in AST index(P<0.01). The Se+900 mg/kg intervention group showed significantly increased AST,TP and GLB(P<0.01). Compared with the treated group,the intervention group was lower than the corresponding exposure group,and the Se+900 mg/kg intervention group showed a significant decrease in TP and GLB indicators(P<0.01). CONCLUSION:Sub-chronic exposure of DEHP affected body weight and growth,and liver function as well as caused liver swelling. The intervention of selenium effectively alleviated the toxic effect of DEHP on the liver.
引文
[1]白小舰.华南地区典型饮用水源地邻苯二甲酸酯的健康风险评估研究[D].兰州:兰州交通大学,2013.
[2]HEUDORF U,MERSCH-SUNDERMANN V,ANGERER J.Phthalates:toxicology and exposure[J].Int J Hyg Environ Health,2007,210(5):623-634.
[3]刘瑞菁,贺永健,刘焕,等.DEHP对大鼠肝组织及肝细胞色素P450酶系的影响[J].中国环境科学,2017,37(7):2749-2754.
[4]LO D,WANG Y T,WU M C.Hepatoprotective effect of silymarin on di(2-ethylhexyl)phthalate(DEHP)induced injury in liver FL83B cells[J].Environ Toxicol Pharmacol,2014,38(1):112-118.
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[6]王媛.富硒酵母对玉米赤霉烯酮致染毒雄性小鼠生殖系统损伤保护作用的研究[D].沈阳:沈阳农业大学,2016.
[7]LIU Z J,FU X,HUANG W,et al.Photodynamic effect and mechanism study of selenium-enriched phycocyanin from Spirulina platensis against liver tumours[J].J Photochem Photobiol B,2018,180(3):89-97.
[8]DALGAARD T S,BRIENS M,ENGBERG R M,et al.The influence of selenium and selenoproteins on immune responses of poultry and pigs[J].Anim Feed Sci Technol,2018,238(4):73-83.
[9]孙建新,安娟,连军.影响实验动物脏器重量及脏器系数因素分析[J].实验动物科学,2009,26(1):49-51.
[10]陈文婕,戴红,陈敏,等.邻苯二甲酸二乙基己酯(DEHP)对小白鼠肝脏毒性及脂质过氧化损伤[J].生态毒理学报,2012,7(1):93-98.
[11]KHATTAB H,FOUAD A,HAMZA M,et al.Relation of ALTand AST levels to the histopathological changes in liver biopsies of patients with chronic hepatitis C genotype 4[J].Arab JGastroenterol,2015,16(2):50-53.
[12]王昕,王岩,秦顺义,等.不同剂量硒蛋白粉对大鼠血常规指标和血液生化指标的影响[J].中国畜牧兽医,2014,41(7):148-152.
[13]GE J,HAN B Y,HU H J,et al.Epigallocatechin-3-O-Gallate Protects Against Hepatic Damage and Testicular Toxicity in Male Mice Exposed to Di-(2-Ethylhexyl)Phthalate[J].J Med Food,2015,18(7):753-761.
[14]罗育春,陈大兰.肝硬化患者血清谷丙转氨酶、谷草转氨酶和总胆红素与肝纤维化标志物的相关性研究[J].实用预防医学,2011,18(8):1542-1544.
[15]邱辅佑,冯学冠,黄慈花.肝病患者血清前白蛋白与白蛋白检测的临床价值[J].中国热带医学,2008,8(11):1927-1928.
[16]CROASDELL A,DUFFNEY PF,KIM N,et al.PPARγand the innate immune system mediate the resolution of inflammation[J/OL].PPAR Res,2015.doi://10.1155/2015/549691.
[17]WANG G,LIU L,ZHANG Y,et al.Activation of PPARγattenuates LPS-induced acute lung injury by inhibition of HMGB1-RAGE levels[J].Eur J Pharmacol,2014,726(3):27-32.
[18]ZHANG WANG,SHEN X Y,ZHANG W W,et al.Di-(2-ethylhexyl)phthalate could disrupt the insulin signaling pathway in l iver o f S D r ats a nd L 02 c ells v ia P PAR g amma[J].T oxicol Appl Pharmacol,2017,316(2):17-26.
[19]张俊杰.硒的生理功能及富硒强化食品的研究进展[J].微量元素与健康研究,2006,23(3):58-60.
[2]HEUDORF U,MERSCH-SUNDERMANN V,ANGERER J.Phthalates:toxicology and exposure[J].Int J Hyg Environ Health,2007,210(5):623-634.
[3]刘瑞菁,贺永健,刘焕,等.DEHP对大鼠肝组织及肝细胞色素P450酶系的影响[J].中国环境科学,2017,37(7):2749-2754.
[4]LO D,WANG Y T,WU M C.Hepatoprotective effect of silymarin on di(2-ethylhexyl)phthalate(DEHP)induced injury in liver FL83B cells[J].Environ Toxicol Pharmacol,2014,38(1):112-118.
[5]CHEN H,ZHANG W,RUI B B,et al.Di(2-ethylhexyl)phthalate exacerbates non-alcoholic fatty liver in rats and its potential mechanisms[J].Environ Toxicol Pharmacol,2016,42(3):38-44.
[6]王媛.富硒酵母对玉米赤霉烯酮致染毒雄性小鼠生殖系统损伤保护作用的研究[D].沈阳:沈阳农业大学,2016.
[7]LIU Z J,FU X,HUANG W,et al.Photodynamic effect and mechanism study of selenium-enriched phycocyanin from Spirulina platensis against liver tumours[J].J Photochem Photobiol B,2018,180(3):89-97.
[8]DALGAARD T S,BRIENS M,ENGBERG R M,et al.The influence of selenium and selenoproteins on immune responses of poultry and pigs[J].Anim Feed Sci Technol,2018,238(4):73-83.
[9]孙建新,安娟,连军.影响实验动物脏器重量及脏器系数因素分析[J].实验动物科学,2009,26(1):49-51.
[10]陈文婕,戴红,陈敏,等.邻苯二甲酸二乙基己酯(DEHP)对小白鼠肝脏毒性及脂质过氧化损伤[J].生态毒理学报,2012,7(1):93-98.
[11]KHATTAB H,FOUAD A,HAMZA M,et al.Relation of ALTand AST levels to the histopathological changes in liver biopsies of patients with chronic hepatitis C genotype 4[J].Arab JGastroenterol,2015,16(2):50-53.
[12]王昕,王岩,秦顺义,等.不同剂量硒蛋白粉对大鼠血常规指标和血液生化指标的影响[J].中国畜牧兽医,2014,41(7):148-152.
[13]GE J,HAN B Y,HU H J,et al.Epigallocatechin-3-O-Gallate Protects Against Hepatic Damage and Testicular Toxicity in Male Mice Exposed to Di-(2-Ethylhexyl)Phthalate[J].J Med Food,2015,18(7):753-761.
[14]罗育春,陈大兰.肝硬化患者血清谷丙转氨酶、谷草转氨酶和总胆红素与肝纤维化标志物的相关性研究[J].实用预防医学,2011,18(8):1542-1544.
[15]邱辅佑,冯学冠,黄慈花.肝病患者血清前白蛋白与白蛋白检测的临床价值[J].中国热带医学,2008,8(11):1927-1928.
[16]CROASDELL A,DUFFNEY PF,KIM N,et al.PPARγand the innate immune system mediate the resolution of inflammation[J/OL].PPAR Res,2015.doi://10.1155/2015/549691.
[17]WANG G,LIU L,ZHANG Y,et al.Activation of PPARγattenuates LPS-induced acute lung injury by inhibition of HMGB1-RAGE levels[J].Eur J Pharmacol,2014,726(3):27-32.
[18]ZHANG WANG,SHEN X Y,ZHANG W W,et al.Di-(2-ethylhexyl)phthalate could disrupt the insulin signaling pathway in l iver o f S D r ats a nd L 02 c ells v ia P PAR g amma[J].T oxicol Appl Pharmacol,2017,316(2):17-26.
[19]张俊杰.硒的生理功能及富硒强化食品的研究进展[J].微量元素与健康研究,2006,23(3):58-60.