栀子醒酒饮品的醒酒及护肝作用
|
摘要
比较不同剂量栀子醒酒饮品对醉酒小鼠爬杆和转轮能力,肝肾病理切片,以及血液中谷草转氨酶(AST)、谷丙转氨酶(ALT)、肝脏中超氧化物歧化酶(SOD)、细胞色素P4502E1(CYP2E1)含量的影响,以考察饮品对醉酒小鼠的醒酒及酒精性肝脏损伤小鼠的护肝作用。转轮结果显示,低、高剂量组掉落次数显著减少、第一次掉落时间增加(p<0.05);爬杆结果显示,低、高剂量组爬杆等级显著降低、爬杆时间增加(p <0.05)。给药组能减少醉酒小鼠肝细胞脂肪性变,减轻肝脏细胞肿胀,缓解炎细胞浸润,减少小鼠肾小管水样性变;与模型组比较,低、中剂量组血液中AST值分别降低了33.13%、29.54%、ALT值分别减少了39.41%、38.91%,高剂量组肝内SOD含量增加了30.34%,给药组对CYP2E1影响较小,上述结果说明该醒酒饮品对醉酒小鼠的有一定的醒酒作用,并对小鼠的酒精性肝脏损伤有一定的保护作用。
In order to investigate the sobering effects of the different doses of sobering drinks and the protection of alcohol-induced liver injury on temulentia mice, the abilities of rods and runners, biochemical and histological examinations of blood, liver and kidney were compared. The results showed that the number of drops and level of climbing in low and high dose groups were less than those in model group(p<0.05), and the first drop time and climbing time were longer than those(p<0.05). Compared with model group, the vacuolar degeneration was reduced, the cell swelling and inflammation were lessened, and the renal tubular water degeneration of kidney was decreased in the drug-administered group. And the values of AST were diseased by 33.13% and 29.54%, as well as the values of ALT were reduced by 39.41%and 38.91% in low and middle dose groups. The content of SOD was increased by 30.34% in high dose group, and the administered groups had less influence on CYP2 E1. The findings indicated that the sobering drink has a certain sobering effect on temulentia mice, and has certain protective effects on alcohol-induced liver injury in mice.
In order to investigate the sobering effects of the different doses of sobering drinks and the protection of alcohol-induced liver injury on temulentia mice, the abilities of rods and runners, biochemical and histological examinations of blood, liver and kidney were compared. The results showed that the number of drops and level of climbing in low and high dose groups were less than those in model group(p<0.05), and the first drop time and climbing time were longer than those(p<0.05). Compared with model group, the vacuolar degeneration was reduced, the cell swelling and inflammation were lessened, and the renal tubular water degeneration of kidney was decreased in the drug-administered group. And the values of AST were diseased by 33.13% and 29.54%, as well as the values of ALT were reduced by 39.41%and 38.91% in low and middle dose groups. The content of SOD was increased by 30.34% in high dose group, and the administered groups had less influence on CYP2 E1. The findings indicated that the sobering drink has a certain sobering effect on temulentia mice, and has certain protective effects on alcohol-induced liver injury in mice.
引文
[1]曹颖,郭倩倩,李廷利,等.瑶山肝宝胶囊对小鼠解酒及防醉作用研究[J].亚太传统医药,2012,8(5):16-17CHAO Ying,GUO Qian-qian,LI Yan-li,Study on the effect of Yaoshan Ganbao capsule on hangover and anti-drunk in mice[J].Asia-Pacific Traditional Medicine,2012,8(5):16-17
[2]吉宁,刘敏,徐建伟,等.有害使用酒精对健康的危害[J].中国慢性病预防与控制,2017,25(9):714-717JI Ning,LIU Min,XU Jian-wei,et al.Harmful use of alcohol for health hazards[J].Chinese Journal of Prevention and Control of Chronic Non-communicable Diseases,2017,25(9):714-717
[3]黄承繁,韦驱,韦为.酒依赖患者记忆状况及其相关因素分析[J].四川精神卫生,2008,3:171-172HUANG Cheng-fan,WEI Qu,WEI Wei.Analysis of memory status and related factors in patients with alcohol dependence[J].Sichuan Mental Healtb,2008,3:171-172
[4]苑函.动物醉酒实验模型的研究[D].北京:中国农业大学,2005YUAN Han.Research on experimental model of drunkenness[D].Beijing:China Agricultural University,2005
[5]刘婕,李晓健,肇晖.下丘脑室旁核中CART 55-102在创伤应激诱导的大鼠饮酒行为中的作用[J].复旦学报(医学版),2018,45(5):682-688LIU Jie,LI Xiao-jian,ZHAO Hui.The role of CART 55-102in hypothalamic paraventricular nucleus on trauma-induced drinking behavior in rats[J].Fudan University Journal of Medical Sciences,2018,45(5):682-688
[6]万亮琴,张子剑,谭琰,等.栀子及栀子苷抗炎作用机制的最新研究进展[J].现代中药研究与实践,2017,31(3):80-83WAN Liang-qin,ZHANG Zi-jian,TAN Yan,et al.Advances in studies on anti-inflammatory mechanism of Gardenia jasminoide and geniposide[J].Research and Practice on Chinese Medicines,2017,31(3):80-83
[7]付田,蒲蔷,谭健,等.栀子京尼平苷对小鼠急性酒精性肝损伤的保护作用[J].中药药理与临床,2007,3:25-27FU Tian,PU Qiang,TAN Jian,et al.Protective effect of scorpion genipin on acute alcoholic liver injury in mice[J].Pharmacology and Clinics of Chinese Materia Medica,2007,3:25-27
[8]张丹参,沈丽霞,薛贵平,等.栀子苷对急性乙醇中毒致小鼠学习记忆障碍的改善作用[J].河北北方学院学报(医学版),2010,27(2):18-20ZHANG Dan-shen,SHEN Li-xia,XUE Gui-ping,et al.The protective effects of geniposide on memory impaired mice induced by acute alcoholic intoxication[J].Journal of Hebei North University(Medical Edition),2010,27(2):18-20
[9]漆乐媛,张风波,肖日传,等.栀子油对小鼠急性酒性肝损伤的保护作用[J].中国油脂,2018,43(8):76-80QI Le-yuan,ZHANG Feng-bo,XIAO Ri-chuan,et al.Protective effect of gardenia oil against alcohol-induced acute hepatic injury in mice[J].China Oils and Fats,2018,43(8):76-80
[10]杨战锋,李晓勇,周百中,等.栀子大黄汤对酒精性脂肪肝大鼠的保作用[J].吉林大学学报(医学版),2017,43(3):555-560,669YANG Zhan-feng,LI Xiao-yong,ZHOU Bai-zhong,et al.Protective effect of Zhizi Dahuangtang on rats with alcoholic fatty liver[J].Journal of Jilin University(Medicine Edition),2017,43(3):555-560,669
[11]曾学文.茵陈栀子汤治疗酒精性肝炎50例[J].现代中西医结合杂志,2010,19(15):1866-1867ZENG Xue-wen.Treatment of 50 cases of alcoholic hepatitis with Yinchenzhizi decoction.[J].Modern Journal of Integrated Traditional Chinese and Western Medicine,2010,19(15):1866-1867
[12]李超,倪宏,王浙东.运动训练对发育期大鼠反复惊厥所致认知损害的干预及机制研究[J].中国康复医学杂志,2010,25(3):205-210LI Chao,NI Hong,WANG Zhe-dong.The long-term effects of physical exercises on recurrent convulsion-induced cognitive deficits in developing rats and it’s mechanism[J].China Journal of Rehabilitation Medicine,2010,25(3):205-210
[13]张凤杰,王德良,李红,等.酒中生物胺对小鼠醉酒行为及脑中5-羟色胺含量的影响[J].酿酒科技,2015,2:40-44ZHANG Feng-jie,WANG De-liang,LI Hong,et al.Effect of biogenic amine in liquor on mice’s drunken behavior and the5-ht content in their brains[J].Liquor-Making Science&Technology,2015,2:40-44
[14]张志毕,张媛,于浩飞,等.余甘子提取物对小鼠急性酒精肝损伤的保护作用研究[J].食品工业科技,2017,38(5):350-356ZHANG Zhi-bi,ZHANG Yuan,YU Hao-fei,et al.Protection and mechanism of extractum phyllanthus emblica on acute alcohol-induced liver injury in mice[J].Science and Technology of Food Industry,2017,38(5):350-356
[15]赵雪珂,程明亮.茅台酒对大鼠肝组织中CYP2E1基因及蛋白质表达的影响[J].贵阳医学院学报,2013,38(06):573-577ZHAO Xue-ke,CHENG Ming-liang.Effect of Maotai liquor on CYP2E1 expression of hepatic tissues of rats[J].J Guiyang Med Coll,2013,38(6):573-577
[16]Tung M L,Natalia N.CYP2E1 and oxidant stress in alcoholic and non-alcoholic fatty liver disease[J].Journal of Hepatology,2013,58(2):395-398
[17]陈浩凡,胡瑜,王权,等.养肝解酒方解酒功能及对急性肝损伤的保护作用[J].中药材,2014,37(4):660-663CHEN Hao-fan,HU Yu,WANG Quan,et al.The function of hangover in nourishing liver and hangover and its protective effect on acute liver injury[J].Journal of Chinese Medicinal Materials,2014,37(4):660-663
[18]Meng X,Zha D,Ma T,et al.Hepatoprotective effects of geniposide in a rat model of nonalcoholic steatohepatitis[J].Journal of Pharmacy and Pharmacology,2011,63(4):587-593
[19]冯筱懿,田婧卓,易艳,等.栀子苷对大鼠的肾脏毒性作用[J].中国实验方剂学杂志,2016,22(10):118-121FENG Xiao-yi,TIAN Jing-zhuo,YI Yan,et al.Nephrotoxicity effect of gardenoside in rats[J].Chinese Journal of Experimental Traditional Medicine,2016,22(10):118-121
[20]王清然,邓中平.栀子肝脏毒性研究进展[J].中成药,2016,38(6):1351-1354WANG Qing-ran,DENG Zhong-ping.Progress in research on liver toxicity of gardenia[J].Chinese Traditional Patent Medicine,2016,38(6):1351-1354
[2]吉宁,刘敏,徐建伟,等.有害使用酒精对健康的危害[J].中国慢性病预防与控制,2017,25(9):714-717JI Ning,LIU Min,XU Jian-wei,et al.Harmful use of alcohol for health hazards[J].Chinese Journal of Prevention and Control of Chronic Non-communicable Diseases,2017,25(9):714-717
[3]黄承繁,韦驱,韦为.酒依赖患者记忆状况及其相关因素分析[J].四川精神卫生,2008,3:171-172HUANG Cheng-fan,WEI Qu,WEI Wei.Analysis of memory status and related factors in patients with alcohol dependence[J].Sichuan Mental Healtb,2008,3:171-172
[4]苑函.动物醉酒实验模型的研究[D].北京:中国农业大学,2005YUAN Han.Research on experimental model of drunkenness[D].Beijing:China Agricultural University,2005
[5]刘婕,李晓健,肇晖.下丘脑室旁核中CART 55-102在创伤应激诱导的大鼠饮酒行为中的作用[J].复旦学报(医学版),2018,45(5):682-688LIU Jie,LI Xiao-jian,ZHAO Hui.The role of CART 55-102in hypothalamic paraventricular nucleus on trauma-induced drinking behavior in rats[J].Fudan University Journal of Medical Sciences,2018,45(5):682-688
[6]万亮琴,张子剑,谭琰,等.栀子及栀子苷抗炎作用机制的最新研究进展[J].现代中药研究与实践,2017,31(3):80-83WAN Liang-qin,ZHANG Zi-jian,TAN Yan,et al.Advances in studies on anti-inflammatory mechanism of Gardenia jasminoide and geniposide[J].Research and Practice on Chinese Medicines,2017,31(3):80-83
[7]付田,蒲蔷,谭健,等.栀子京尼平苷对小鼠急性酒精性肝损伤的保护作用[J].中药药理与临床,2007,3:25-27FU Tian,PU Qiang,TAN Jian,et al.Protective effect of scorpion genipin on acute alcoholic liver injury in mice[J].Pharmacology and Clinics of Chinese Materia Medica,2007,3:25-27
[8]张丹参,沈丽霞,薛贵平,等.栀子苷对急性乙醇中毒致小鼠学习记忆障碍的改善作用[J].河北北方学院学报(医学版),2010,27(2):18-20ZHANG Dan-shen,SHEN Li-xia,XUE Gui-ping,et al.The protective effects of geniposide on memory impaired mice induced by acute alcoholic intoxication[J].Journal of Hebei North University(Medical Edition),2010,27(2):18-20
[9]漆乐媛,张风波,肖日传,等.栀子油对小鼠急性酒性肝损伤的保护作用[J].中国油脂,2018,43(8):76-80QI Le-yuan,ZHANG Feng-bo,XIAO Ri-chuan,et al.Protective effect of gardenia oil against alcohol-induced acute hepatic injury in mice[J].China Oils and Fats,2018,43(8):76-80
[10]杨战锋,李晓勇,周百中,等.栀子大黄汤对酒精性脂肪肝大鼠的保作用[J].吉林大学学报(医学版),2017,43(3):555-560,669YANG Zhan-feng,LI Xiao-yong,ZHOU Bai-zhong,et al.Protective effect of Zhizi Dahuangtang on rats with alcoholic fatty liver[J].Journal of Jilin University(Medicine Edition),2017,43(3):555-560,669
[11]曾学文.茵陈栀子汤治疗酒精性肝炎50例[J].现代中西医结合杂志,2010,19(15):1866-1867ZENG Xue-wen.Treatment of 50 cases of alcoholic hepatitis with Yinchenzhizi decoction.[J].Modern Journal of Integrated Traditional Chinese and Western Medicine,2010,19(15):1866-1867
[12]李超,倪宏,王浙东.运动训练对发育期大鼠反复惊厥所致认知损害的干预及机制研究[J].中国康复医学杂志,2010,25(3):205-210LI Chao,NI Hong,WANG Zhe-dong.The long-term effects of physical exercises on recurrent convulsion-induced cognitive deficits in developing rats and it’s mechanism[J].China Journal of Rehabilitation Medicine,2010,25(3):205-210
[13]张凤杰,王德良,李红,等.酒中生物胺对小鼠醉酒行为及脑中5-羟色胺含量的影响[J].酿酒科技,2015,2:40-44ZHANG Feng-jie,WANG De-liang,LI Hong,et al.Effect of biogenic amine in liquor on mice’s drunken behavior and the5-ht content in their brains[J].Liquor-Making Science&Technology,2015,2:40-44
[14]张志毕,张媛,于浩飞,等.余甘子提取物对小鼠急性酒精肝损伤的保护作用研究[J].食品工业科技,2017,38(5):350-356ZHANG Zhi-bi,ZHANG Yuan,YU Hao-fei,et al.Protection and mechanism of extractum phyllanthus emblica on acute alcohol-induced liver injury in mice[J].Science and Technology of Food Industry,2017,38(5):350-356
[15]赵雪珂,程明亮.茅台酒对大鼠肝组织中CYP2E1基因及蛋白质表达的影响[J].贵阳医学院学报,2013,38(06):573-577ZHAO Xue-ke,CHENG Ming-liang.Effect of Maotai liquor on CYP2E1 expression of hepatic tissues of rats[J].J Guiyang Med Coll,2013,38(6):573-577
[16]Tung M L,Natalia N.CYP2E1 and oxidant stress in alcoholic and non-alcoholic fatty liver disease[J].Journal of Hepatology,2013,58(2):395-398
[17]陈浩凡,胡瑜,王权,等.养肝解酒方解酒功能及对急性肝损伤的保护作用[J].中药材,2014,37(4):660-663CHEN Hao-fan,HU Yu,WANG Quan,et al.The function of hangover in nourishing liver and hangover and its protective effect on acute liver injury[J].Journal of Chinese Medicinal Materials,2014,37(4):660-663
[18]Meng X,Zha D,Ma T,et al.Hepatoprotective effects of geniposide in a rat model of nonalcoholic steatohepatitis[J].Journal of Pharmacy and Pharmacology,2011,63(4):587-593
[19]冯筱懿,田婧卓,易艳,等.栀子苷对大鼠的肾脏毒性作用[J].中国实验方剂学杂志,2016,22(10):118-121FENG Xiao-yi,TIAN Jing-zhuo,YI Yan,et al.Nephrotoxicity effect of gardenoside in rats[J].Chinese Journal of Experimental Traditional Medicine,2016,22(10):118-121
[20]王清然,邓中平.栀子肝脏毒性研究进展[J].中成药,2016,38(6):1351-1354WANG Qing-ran,DENG Zhong-ping.Progress in research on liver toxicity of gardenia[J].Chinese Traditional Patent Medicine,2016,38(6):1351-1354