高容量阴离子交换磁性微球的制备及其对蛋白质的吸附性能
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摘要
为了获得高容量的阴离子交换吸附剂,本研究以Fe_3O_4磁性纳米粒子为基质,在其表面包覆聚多巴胺,然后与树枝状大分子聚乙烯亚胺反应,制得多氨基化磁性微球。此微球的离子交换容量为9.1 mmol/g。采用透射电镜、红外光谱和热重分析对材料进行了表征。以β-酪蛋白和牛血清蛋白(BSA)为模型蛋白,采用静态吸附法研究了此微球对蛋白质的吸附性能,在最佳条件下测定了微球对蛋白质的静态吸附等温线。结果表明,此微球对蛋白质的最佳吸附时间为2h,缓冲溶液的最佳pH值为7.0。在此条件下,对β-酪蛋白和BSA的最大吸附容量分别为237.5和204.5μg/mg,而对溶菌酶和核糖核酸酶A几乎不产生吸附。实验结果表明,此微球对酸性蛋白具有选择性,在蛋白质分离纯化方面具有良好的应用前景。
A high capacity anion exchange magnetic absorbent was prepared by immobilization of hyperbranched polyethyleneimine( PEI) on polydopamine-coated Fe_3O_4 microspheres(Fe_3O_4@pDA) and the ion exchange capacity was determined as 9.1 mmol/g. The absorbent was characterized by transmission electron microscope( TEM),infrared spectroscopy( IR) and thermogravimetric analysis( TGA). Moreover,the adsorption behavior of the absorbent for protein was carefully investigated by static adsorption using bovine serum albumin( BSA) and β-casein as models and then the static isotherms were determined under the optimum conditions. It was shown that the highest adsorption amount for protein was obtained when the adsorption time was 2h and the p H was 7.0. Under the optimum conditions,the saturated adsorption capacity towards β-casein and BSA was determined as high as 237.5 and 204.5 μg/mg,respectively. Comparatively,the adsorbent exhibited little adsorption towards lysozyme and Rnase A,showing highly selectivity towards acidic protein. Therefore,the adsorbent exhibited potential in the separation and purification of protein from real samples.
A high capacity anion exchange magnetic absorbent was prepared by immobilization of hyperbranched polyethyleneimine( PEI) on polydopamine-coated Fe_3O_4 microspheres(Fe_3O_4@pDA) and the ion exchange capacity was determined as 9.1 mmol/g. The absorbent was characterized by transmission electron microscope( TEM),infrared spectroscopy( IR) and thermogravimetric analysis( TGA). Moreover,the adsorption behavior of the absorbent for protein was carefully investigated by static adsorption using bovine serum albumin( BSA) and β-casein as models and then the static isotherms were determined under the optimum conditions. It was shown that the highest adsorption amount for protein was obtained when the adsorption time was 2h and the p H was 7.0. Under the optimum conditions,the saturated adsorption capacity towards β-casein and BSA was determined as high as 237.5 and 204.5 μg/mg,respectively. Comparatively,the adsorbent exhibited little adsorption towards lysozyme and Rnase A,showing highly selectivity towards acidic protein. Therefore,the adsorbent exhibited potential in the separation and purification of protein from real samples.
引文
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2 Farrokhzad H,Kikhavani T,Monnaie F,Ashrafizadeh S N,Koeckelberghs G,Gerven T V,Bruggen B V.J.Mem.Sci.,2015,474:167-174
3 LI Jing,ZHU Yan.Chinese J.Anal.Chem.,2013,41(3):400-405李晶,朱岩.分析化学,2013,41(3):400-405
4 Wang W,He M,Wang C,Wei Y.Anal.Chim.Acta,2015,886:66-74
5 XU Li-Li,Zh ONG Ming-Hua,CHEN Xiao-Jing.Chinese Journal of Chromatography,2015,5:461-467许丽丽,衷明华,陈小静.色谱,2015,5:461-467
6 Li X,Liu Y,Sun Y.Biochem.Eng.J.,2017,126:50-57
7 LI Yuan-Yuan,WEI Man-Man,CHEN Dong,MA Yu-Long.Chinese J.Anal.Chem.,2015,43(6):876-880李媛媛,韦嫚嫚,陈桐,马玉龙.分析化学,2015,43(6):876-880
8 WANG Fu-Qiang,CHANG Su-Ping,DAI Xiao-Jun,GONG Bo-Lin.Chinese J.Anal.Chem.,2013,41(3):415-420王富强,常素萍,戴小军,龚波林.分析化学,2013,41(3):415-420
9 Li S,Sun Y,Shi Q H.Biochem.Eng.J.,2015,103:122-129
10 Meng J,Cao J,Xu R,Wang Z,Sun R.J.Mater.Chem.A,2016,4(30):11656-11665
11 Wang C,Sun Y.J.Chromatogr.A,2013,1307:73-79
12 Tao S,Wang C,Sun Y.J.Chromatogr.A,2014,1359:76-83
13 Yu LL,Tao S P,Dong X Y,Sun Y.J.Chromatogr.A,2013,1305:76-84
14 Hong Y,Liu N,Wei W,Yu L,Ma G,Sun Y.J.Chromatogr.A,2014,1342:30-36
15 Zhang R,Li Q,Gao Y,Li J,Huang Y,Song C,Zhou W,Ma G,Su Z.J.Chromatogr.A,2014,1343:109-118
16 Xu L,Qi X,Li X,Bai Y,Liu H.Talanta,2016,146:714-726
17 SUN Lin-Lin,ZHOU Ye-Hong,WANG Fei,SHUANG Shao-Min,DONG Chuan.Chinese J.Appl.Chem.,2015,32(1):110-117孙琳琳,周叶红,王斐,双少敏,董川.应用化学,2015,32(1):110-117
18 SHAN Bing-Hui,WANG Chao-Zhan,WEI Yin-Mao.Chinese J.Anal.Chem.,2016,44(7):1119-1124单丙辉,王超展,卫引茂.分析化学,2016,44(7):1119-1124
19 Zhu H,Yao H,Xia K,Liu J,Yin X,Zhang W,Pan J.Chem.Eng.J.,2018,346:317-328
20 Jiang D,Li X,Ma J,Jia Q.Talanta,2018,180:368-375
21 Chen Y,Xiong Z,Peng L,Gan Y,Zhao Y,Shen J,Qian J,Zhang L,Zhang W.ACS Appl.Mater.Interfaces,2015,7(30):16338-16347
22 Fang W,Chen X,Zheng N.J.Mater.Chem.,2010,20(39):8624-8630
23 Liu R,Guo Y,Odusote G,Qu F,Priestley R.ACS Appl.Mater.Interfaces,2013,5:9167-9171
24 Deng S,Ting Y P.Langmuir,2005,21:5940-5948
25 Liu Y,Ai K,Lu L.Chem.Rev.,2014,114:5057-5115
26 Li Q X,Wen J Y,Koon G N,En T K,Guo D F.Macromolecules,2010,43:8336-8339
27 Cao J,Zhang X,He X,Chen L,Zhang Y.J.Mater.Chem.B,2013,1:3625-3632
28 SU Ri-Na,XIE Ming-Xue,LI Hai-Jie,DENG Qi-Liang.Chinese Journal of Chromatography,2016,34(6):545-549苏日娜,谢明雪,李海杰,邓启良.色谱,2016,34(6):545-549
29 WANG E-Na,DUAN Shu-Jing,HE Xi-Wen,CHEN Lang-Xin,LI Wen-You,ZHANG Yu-Kui.Acta Chimica Sinica,2010,68(23):2421-2428王婀娜,段淑静,何锡文,陈朗星,李文友,张玉奎.化学学报,2010,68(23):2421-2428
30 LU Hui-Li,LIN Dong-Qiang,YAO Shan-Jing.Journal of Chemical Industry and Engineering(China),2011,62(11):3165-3170卢慧丽,林东强,姚善泾.化工学报,2011,62(11):3165-3170