三白草不同提取物抗肠道病毒71型活性的研究
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摘要
目的:研究三白草不同提取物的抗病毒活性。方法:采用细胞体外实验的方法,应用CPE结合MTT的方法,以半数细胞毒性浓度(TC50)、半数治疗浓度(EC50)治疗指数(TI值)为研究目标,以利巴韦林为阳性对照药,研究三白草不同提取物对肠道病毒71型(EV71)的抑制作用。结果:高温水浸泡提取、水超声提取、75%乙醇超声提取和丙酮冷浸提取的四种供试品的治疗指数分别是211. 09、9. 72、43. 69、8. 36。阳性对照利巴韦林的治疗指数为28. 36。结论:四种供试品在体外均对EV71有抑制作用,其中以高温水浸泡提取的效果最好。
Objective: To explore the antiviral activity of different extractions of Saururus chinensis( Lour.) Baill.Methods: We investigated the antiviral activity of different extractions of Saururus chinensis( Lour.) Baill through evaluating the half cell toxicity concentration( TC50),half therapeutic concentration(EC50) and therapeutic index(TI) of each extractions in EV71 infected models by using the cytopathic effects( CPE) and MTT assays. Ribavirin was served as a positive reference drug. Results: All the extractons of Saururus chinensis( Lour.) Baill had inhibitory effect on EV71 infection. The TC50 of hot water extraction,water ultrasound extraction,75% alcohol ultrasound extraction and acetone soaking extraction was 2. 74 mg/m L,2. 84 mg/m L,2. 89 mg/m L,2. 18 mg/m L,respectively. EC50 was 12. 98 μg/m L,292. 32 μg/m L,66. 14 μg/m L,260. 85 μg/m L,respectively. TI was 211. 09,9. 72,43. 69,8. 36,respectively. Conclusion: All the extractions of Saururus chinensis( Lour.) Baill can block EV71 infection. Among the four extractions,hot water extraction possessed the most effective antiviral activity with a TI value of 211. 09,which was much more higher than those of the positive control of ribavirin.
Objective: To explore the antiviral activity of different extractions of Saururus chinensis( Lour.) Baill.Methods: We investigated the antiviral activity of different extractions of Saururus chinensis( Lour.) Baill through evaluating the half cell toxicity concentration( TC50),half therapeutic concentration(EC50) and therapeutic index(TI) of each extractions in EV71 infected models by using the cytopathic effects( CPE) and MTT assays. Ribavirin was served as a positive reference drug. Results: All the extractons of Saururus chinensis( Lour.) Baill had inhibitory effect on EV71 infection. The TC50 of hot water extraction,water ultrasound extraction,75% alcohol ultrasound extraction and acetone soaking extraction was 2. 74 mg/m L,2. 84 mg/m L,2. 89 mg/m L,2. 18 mg/m L,respectively. EC50 was 12. 98 μg/m L,292. 32 μg/m L,66. 14 μg/m L,260. 85 μg/m L,respectively. TI was 211. 09,9. 72,43. 69,8. 36,respectively. Conclusion: All the extractions of Saururus chinensis( Lour.) Baill can block EV71 infection. Among the four extractions,hot water extraction possessed the most effective antiviral activity with a TI value of 211. 09,which was much more higher than those of the positive control of ribavirin.
引文
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[7]Jisuk L,Myoungsook H,Youngchoong K,et al. Lignan,sesquilignans and dilignans,novel HIV-1 protease and cytopathic effect inhibitors purified from the rhizomes of Saururus chinensis.[J]. Antiviral Research,2010,85(2):425-428.
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[11]陈宏降,王绪楠,陈建伟,等.三白草酮超临界CO2萃取工艺优化及三白草超临界萃取物体外抗肿瘤活性[J].中成药,2017,39(8):1601-1604.
[12]Cui H,Xu B,Wu T,et al. Potential antiviral lignans from the roots of Saururus chinensis with activity against Epstein-Barr virus lytic replication[J]. Journal of Natural Products,2014,77(1):100-110.
[13]Li Z H,Li C M,Ling P,et al. Ribavirin Reduces Mortality in Enterovirus 71-Infected Mice by Decreasing Viral Replication[J]. Journal of Infectious Diseases,2008,197(6):854-857.
[14]Zhang G,Zhou F,Gu B,et al. In vitro and in vivo evaluation of ribavirin and pleconaril antiviral activity against enterovirus 71 infection[J]. Archives of Virology,2012,157(4):669-679.
[15]Kamar N,Izopet J,Tripon S,et al. Ribavirin for chronic hepatitis E virus infection in transplant recipients[J]. New England Journal of Medicine,2014,370(12):1111-1120.
[16]Jiang Y C,Hui F,Lin Y C,et al. New strategies against drug resistance to herpes simplex virus[J]. Journal of oral science,2016,8(1):1-6.
[17]Law A H,Yang C L,Lau A S,et al. Antiviral effect of forsythoside A from Forsythia suspensa(Thunb.)Vahl fruit against influenza A virus through reduction of viral M1 protein[J].Journal of Ethnopharmacology,2017,209:236-247.
[18]Peng M,Watanabe S,Chan K W,et al. Luteolin restricts dengue virus replication through inhibition of the proprotein convertase furin[J]. Antiviral Research,2017,143:176-185.
[19]Zhang Q,Zhao B,Chen X,et al. GS-9620 inhibits enterovirus 71 replication mainly through the NF-κB and PI3K-AKT signaling pathways[M]. Antiviral Res,2018.
[20]Shao P,Wu X,Li H,et al. Clinical significance of inflammatory cytokine and chemokine expression in hand,foot and mouth disease[J]. Molecular Medicine Reports,2017,15(5):2859-2866.
[21]Khong W X,Foo D G,Trasti S L,et al. Sustained high levels of interleukin-6 contribute to the pathogenesis of enterovirus71 in a neonate mouse model[J]. Journal of Virology,2011,85(7):3067-3076.
[2]Lee K Y. Enterovirus 71 infection and neurological complications[J]. Korean Journal of Pediatrics,2016,59(10):395-401.
[3]Cox J A,Hiscox J A,Solomon T,et al. Immunopathogenesis and Virus-Host Interactions of Enterovirus 71 in Patients with Hand,Foot and Mouth Disease[M]. Frontiers in Microbiology,2017:8.
[4]Yi E J,Shin Y J,Kim J H,et al. Enterovirus 71 infection and vaccines.[J]. Clinical&Experimental Vaccine Research,2017,6(1):4-14.
[5]Choi M,Kim E,Lee H,et al. Inhibitory effects of Saururus chinensis(LOUR.)BAILL on the development of atopic dermatitis-like skin lesions in NC/Nga mice[J]. Biological&Pharmaceutical Bulletin,2008,31(1):51-56.
[6]陈宏降,陈建伟,李祥.三白草不同药用部位HPLC抗炎药效指纹图谱研究[J].中药材,2017,9(40):2116-2120.
[7]Jisuk L,Myoungsook H,Youngchoong K,et al. Lignan,sesquilignans and dilignans,novel HIV-1 protease and cytopathic effect inhibitors purified from the rhizomes of Saururus chinensis.[J]. Antiviral Research,2010,85(2):425-428.
[8]田蕾,李晓艳,徐云霞,等.三白草抗单纯疱疹病毒作用及机制[J].中国中药杂志,2012,37(11):1642-1645.
[9]Chen X,Wang C,Xu L,et al. A Laboratory Evaluation of Medicinal Herbs Used in China for the Treatment of Hand,Foot,and Mouth Disease[J]. Evidence-Based Complementray and Alternative Medicine,2013(1):504-563.
[10]国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2015:12,224.
[11]陈宏降,王绪楠,陈建伟,等.三白草酮超临界CO2萃取工艺优化及三白草超临界萃取物体外抗肿瘤活性[J].中成药,2017,39(8):1601-1604.
[12]Cui H,Xu B,Wu T,et al. Potential antiviral lignans from the roots of Saururus chinensis with activity against Epstein-Barr virus lytic replication[J]. Journal of Natural Products,2014,77(1):100-110.
[13]Li Z H,Li C M,Ling P,et al. Ribavirin Reduces Mortality in Enterovirus 71-Infected Mice by Decreasing Viral Replication[J]. Journal of Infectious Diseases,2008,197(6):854-857.
[14]Zhang G,Zhou F,Gu B,et al. In vitro and in vivo evaluation of ribavirin and pleconaril antiviral activity against enterovirus 71 infection[J]. Archives of Virology,2012,157(4):669-679.
[15]Kamar N,Izopet J,Tripon S,et al. Ribavirin for chronic hepatitis E virus infection in transplant recipients[J]. New England Journal of Medicine,2014,370(12):1111-1120.
[16]Jiang Y C,Hui F,Lin Y C,et al. New strategies against drug resistance to herpes simplex virus[J]. Journal of oral science,2016,8(1):1-6.
[17]Law A H,Yang C L,Lau A S,et al. Antiviral effect of forsythoside A from Forsythia suspensa(Thunb.)Vahl fruit against influenza A virus through reduction of viral M1 protein[J].Journal of Ethnopharmacology,2017,209:236-247.
[18]Peng M,Watanabe S,Chan K W,et al. Luteolin restricts dengue virus replication through inhibition of the proprotein convertase furin[J]. Antiviral Research,2017,143:176-185.
[19]Zhang Q,Zhao B,Chen X,et al. GS-9620 inhibits enterovirus 71 replication mainly through the NF-κB and PI3K-AKT signaling pathways[M]. Antiviral Res,2018.
[20]Shao P,Wu X,Li H,et al. Clinical significance of inflammatory cytokine and chemokine expression in hand,foot and mouth disease[J]. Molecular Medicine Reports,2017,15(5):2859-2866.
[21]Khong W X,Foo D G,Trasti S L,et al. Sustained high levels of interleukin-6 contribute to the pathogenesis of enterovirus71 in a neonate mouse model[J]. Journal of Virology,2011,85(7):3067-3076.