生物信息学法分析葛根芩连汤治疗心血管系统疾病的分子机理
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摘要
目的利用生物信息学法探讨葛根芩连汤治疗心血管系统疾病的分子机理。方法利用在线数据库检索葛根芩连汤组方中药所含化合物,采用Pub Chem筛选化合物对应的靶蛋白,将靶蛋白汇总后导入蛋白相互作用分析系统,分析靶蛋白涉及的心血管疾病相关分子网络及生物学通路。结果共筛选得到了265个葛根芩连汤靶蛋白,构建得到两个与心血管疾病相关的分子网络,15条涉及心血管疾病的生物学通路,其中,显著性最高的3个生物学通路为Endothelin-1 signaling、Renin-angiotensin signaling、P2Y purigenic receptor signaling pathway。结论葛根芩连汤能够通过多靶点、多途径改善心血管系统病变,分析结果为深入阐明葛根芩连汤治疗心血管疾病的分子机理奠定基础,并提供了进一步研究的参考方向。
OBJECTIVE To explore the molecular mechanism of Gegen qinlian decoction in treatment of cardiovascular diseases by Bioinformatic analysis method. METHODS Focusing on the relationship between Gegen qinlian and angiocardiopathy,compounds and their target proteins were retrieved and gathered,then IPA was used to build the target proteins interaction network as well as biological pathways. RESULTS A total of 265 target proteins were collected,and 2 molecular interaction networks and 15 biological pathways were built which were related cardiovascular diseases. The top 3 biological pathways were Endothelin-1 signaling,Renin-angiotensin signaling and P2 Y purigenic receptor signaling pathway.CONCLUSION Gegenqinlian decoction can improve pathological changes of cardiovascular system through multi-target and pathways. The results provided the theoretical basis and reference for revealing the molecular mechanism of Gegen qinlian decoction in treatment of cardiovascular diseases.
OBJECTIVE To explore the molecular mechanism of Gegen qinlian decoction in treatment of cardiovascular diseases by Bioinformatic analysis method. METHODS Focusing on the relationship between Gegen qinlian and angiocardiopathy,compounds and their target proteins were retrieved and gathered,then IPA was used to build the target proteins interaction network as well as biological pathways. RESULTS A total of 265 target proteins were collected,and 2 molecular interaction networks and 15 biological pathways were built which were related cardiovascular diseases. The top 3 biological pathways were Endothelin-1 signaling,Renin-angiotensin signaling and P2 Y purigenic receptor signaling pathway.CONCLUSION Gegenqinlian decoction can improve pathological changes of cardiovascular system through multi-target and pathways. The results provided the theoretical basis and reference for revealing the molecular mechanism of Gegen qinlian decoction in treatment of cardiovascular diseases.
引文
[1]陈丽红,唐于平,王强.葛根芩连汤的现代研究进展[J].中草药,2010,41(4):附8-附12.
[2]张晓菲,吕冠华.葛根芩连汤临床应用概述[J].中医药临床杂志,2016,28(12):1818-1820.
[3]侯敏娜,侯少平,刘峰,等.基于整合药理学平台的反左金丸镇痛作用的分子机制研究[J].华西药学杂志,2018,33(5):544-547.
[4]梁光义,曹佩分,徐必学,等. HPLC测定葛根芩连汤不同煎液中葛根素的含量[J].华西药学杂志,2001,16(5):371-373.
[5]李洪涛,吴宗贵.环氧合酶与动脉粥样硬化[J].心血管病学进展,2004,25(1):62-65.
[6]吴应涛,谭华清,唐湘宇. NF-κB心血管疾病中的双重作用因子[J].中国循证心血管医学杂志,2017,9(3):371-373.
[7]韩雨薇,张媛媛,赖秀英,等.中药对一氧化氮合酶/一氧化氮系统调节作用的研究进展[J].中国药科大学学报,2017,48(1):8-15.
[8]秦春焕,曹衡,李红莉. PPARβ/δ在兔动脉粥样硬化模型中的表达及作用[J].中国分子心脏病学杂志,2014,14(5):1080-1084.
[9]化冰,赵慧强,李虹伟.高敏心肌肌钙蛋白在冠心病中的应用研究进展[J].心血管病学进展,2017,38(1):15-20.
[10]蔡景景,富路.肝X受体与心力衰竭[J].心血管病学进展,2017,38(1):119-122.
[11]王新,徐锦涛,孙书芳,等.葛根芩连汤及葛根素对脂多糖诱导小鼠小肠粘膜微血管内皮细胞分泌一氧化氮和内皮素的影响[J].中国预防兽医学报,2017,39(4):277-280.
[12]林善锬.值得关注的几个肾素血管紧张素系统新进展[J].中华高血压杂志,2017,25(3):213-215.
[13]李颖,宋淑亮,梁浩,等. P2Y12受体及其抑制剂与急性冠脉综合症[J].生命的化学,2016,36(5):639-644.
[2]张晓菲,吕冠华.葛根芩连汤临床应用概述[J].中医药临床杂志,2016,28(12):1818-1820.
[3]侯敏娜,侯少平,刘峰,等.基于整合药理学平台的反左金丸镇痛作用的分子机制研究[J].华西药学杂志,2018,33(5):544-547.
[4]梁光义,曹佩分,徐必学,等. HPLC测定葛根芩连汤不同煎液中葛根素的含量[J].华西药学杂志,2001,16(5):371-373.
[5]李洪涛,吴宗贵.环氧合酶与动脉粥样硬化[J].心血管病学进展,2004,25(1):62-65.
[6]吴应涛,谭华清,唐湘宇. NF-κB心血管疾病中的双重作用因子[J].中国循证心血管医学杂志,2017,9(3):371-373.
[7]韩雨薇,张媛媛,赖秀英,等.中药对一氧化氮合酶/一氧化氮系统调节作用的研究进展[J].中国药科大学学报,2017,48(1):8-15.
[8]秦春焕,曹衡,李红莉. PPARβ/δ在兔动脉粥样硬化模型中的表达及作用[J].中国分子心脏病学杂志,2014,14(5):1080-1084.
[9]化冰,赵慧强,李虹伟.高敏心肌肌钙蛋白在冠心病中的应用研究进展[J].心血管病学进展,2017,38(1):15-20.
[10]蔡景景,富路.肝X受体与心力衰竭[J].心血管病学进展,2017,38(1):119-122.
[11]王新,徐锦涛,孙书芳,等.葛根芩连汤及葛根素对脂多糖诱导小鼠小肠粘膜微血管内皮细胞分泌一氧化氮和内皮素的影响[J].中国预防兽医学报,2017,39(4):277-280.
[12]林善锬.值得关注的几个肾素血管紧张素系统新进展[J].中华高血压杂志,2017,25(3):213-215.
[13]李颖,宋淑亮,梁浩,等. P2Y12受体及其抑制剂与急性冠脉综合症[J].生命的化学,2016,36(5):639-644.