贻贝粘蛋白降解的关键酶
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摘要
针对贻贝粘蛋白产品的应用范围,对于评分中的蛋白水解环境进行了文献调研,结果显示,丝氨酸蛋白酶是皮肤中主要的蛋白酶类,表明可以利用胰蛋白酶对于贻贝粘蛋白的降解特性进行研究,为贻贝粘蛋白产品开发和应用提供依据。
According to the application scope of mussel adhesive protein products, the environment of protein hydrolysis in skin was investigated. Results showed that serine protease is the major enzyme in the skin. So trypsin can be used to study the degradation of mussel adhesive protein. It will provide basic data for the development and application of mussel adhesive protein.
According to the application scope of mussel adhesive protein products, the environment of protein hydrolysis in skin was investigated. Results showed that serine protease is the major enzyme in the skin. So trypsin can be used to study the degradation of mussel adhesive protein. It will provide basic data for the development and application of mussel adhesive protein.
引文
[1]Haeshin L,Norbert F.S,Phillip B.M.Single-molecule mechanics of mussel adhesion[J].Pnas,206,103(35):129-1303.
[2]Waite J.H,Benedict C.V.Asay of Dihydroxyphenylalanine(Dopa)in Invertebrate Structural Proteins[J].Methods in Enzymology,1984,107:397-413.
[3]Yu M,HWANG J,DEMING T J.Role of L-3,4-Dihydroxyphenylalanine in Mussel Adhesive Proteins[J].J Am Chem Soc,19,121:5825-5826.
[4]胡学智,王俊.蛋白酶生产和应用的进展[J].工业微生物,2008,38(4).
[5]徐晓峰(导师:冷云伟).重组胰蛋白酶大肠杆菌的发酵与表达[D].中国矿业大学硕士论文,2016.
[6]De Veer,Simon J,Furio,et al.Proteases:common culprits in human skin disorders.Trends in Molecular Medicine,2014,20(3):166-178.
[7]Kasparek P,et al.KLK5 and KLK7 Ablation Fully Rescues Lethality of Netherton Syndrome-Like Phenotype[J].PLOSGenetics,2017,13,1.
[8]Prassas I.,Eissa A.,Poda G.,et al.Unleashing the therapeutic potential of human kallikrein-related serine proteases[J].Nature Reviews Drug Discovery,2015(14):183-202.
[9]邓菊云.微生物碱性蛋白酶研究进展[J].现代食品科技,2008,24(3).
[10]汪世华,王文勇,黄益洲,等.丝氨酸蛋白酶研究进展[J].福建农业学报,2007,22(4):453-456.
[2]Waite J.H,Benedict C.V.Asay of Dihydroxyphenylalanine(Dopa)in Invertebrate Structural Proteins[J].Methods in Enzymology,1984,107:397-413.
[3]Yu M,HWANG J,DEMING T J.Role of L-3,4-Dihydroxyphenylalanine in Mussel Adhesive Proteins[J].J Am Chem Soc,19,121:5825-5826.
[4]胡学智,王俊.蛋白酶生产和应用的进展[J].工业微生物,2008,38(4).
[5]徐晓峰(导师:冷云伟).重组胰蛋白酶大肠杆菌的发酵与表达[D].中国矿业大学硕士论文,2016.
[6]De Veer,Simon J,Furio,et al.Proteases:common culprits in human skin disorders.Trends in Molecular Medicine,2014,20(3):166-178.
[7]Kasparek P,et al.KLK5 and KLK7 Ablation Fully Rescues Lethality of Netherton Syndrome-Like Phenotype[J].PLOSGenetics,2017,13,1.
[8]Prassas I.,Eissa A.,Poda G.,et al.Unleashing the therapeutic potential of human kallikrein-related serine proteases[J].Nature Reviews Drug Discovery,2015(14):183-202.
[9]邓菊云.微生物碱性蛋白酶研究进展[J].现代食品科技,2008,24(3).
[10]汪世华,王文勇,黄益洲,等.丝氨酸蛋白酶研究进展[J].福建农业学报,2007,22(4):453-456.