藏系绵羊mtDNA和Y染色体遗传多样性的检测与系统进化关系的分析
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摘要
为了评估青海地区藏系绵羊各类群的遗传结构与分子进化关系,试验选择青海地区四个典型的地理生态类群(山谷型、欧拉型、高原型和扎什加型)藏羊,提取血液基因组DNA后,分别对线粒体D-loop区序列和Y染色体Sry基因序列进行特异性扩增并测序。结果表明:D-loop序列的核苷酸多样性为0.031±0.004,单倍型多样性为0.994±0.006;而Sry序列的核苷酸多样性为0.046±0.005,单倍体多样性为0.861±0.044。说明高原型藏羊在母系遗传上更接近扎什加型藏羊,而在父系遗传上扎什加型藏羊与其他三个类群间的隔离较为明显,三个类群在父系遗传上的基因交流更为频繁。
To evaluate the genetic structure and phylogenic relationships of Tibetan sheep breeds in Qinghai Plateau, the study obtained blood samples from four distributed geographic ecotype populations(Valley Type,Euler Type, Plateau Type and Zhashijia Type). The displacement-loop(D-loop) region on mitochondrial DNA(mtDNA) and the sex determining region Y(Sry) gene sequences on Y chromosome were cloned and sequenced. The results showed that the nucleotide diversity and haplotype diversity were 0.031±0.004 and 0.994±0.006 based on D-loop sequences, and 0.046±0.005 and 0.861±0.044 based on Sry sequences, respectively. The phylogenetic analysis revealed that the Plateau type populations were closely related to Zhashijia Type populations maternally, while Zhashijia Type populations exhibited obvious isolation with other three populations paternally. In contrast, the gene communication among three populations more frequently in the patrilineal genetics.
To evaluate the genetic structure and phylogenic relationships of Tibetan sheep breeds in Qinghai Plateau, the study obtained blood samples from four distributed geographic ecotype populations(Valley Type,Euler Type, Plateau Type and Zhashijia Type). The displacement-loop(D-loop) region on mitochondrial DNA(mtDNA) and the sex determining region Y(Sry) gene sequences on Y chromosome were cloned and sequenced. The results showed that the nucleotide diversity and haplotype diversity were 0.031±0.004 and 0.994±0.006 based on D-loop sequences, and 0.046±0.005 and 0.861±0.044 based on Sry sequences, respectively. The phylogenetic analysis revealed that the Plateau type populations were closely related to Zhashijia Type populations maternally, while Zhashijia Type populations exhibited obvious isolation with other three populations paternally. In contrast, the gene communication among three populations more frequently in the patrilineal genetics.
引文
[1] ZHAO Y, ZHAO E, ZHANG N, et al. Mitochondrial DNA diversity, origin, and phylogenic relationships of three Chinese large-fat-tailed sheep breeds [J]. Trop Anim Health Prod,2011,43(7):1405-1410.
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[5] GUANGXIN E, YONG-JU Z, RI-SU N, et al. Meta-analysis evidence of maternal lineages in Chinese Tibetan sheep using mtDNA D-loop panel [J]. Mitochondrial DNA A DNA Mapp Seq Anal, 2017,28(4):579-583.
[6] NIEMI M,BL?UER A,ISO-TOURU T, et al. Mitochondrial DNA and Y-chromosomal diversity in ancient populations of domestic sheep (ovis aries) in Finland: comparison with contemporary sheep breeds [J]. Genet Sel Evol,2013,45:2.
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[8] CHEN S Y, DUAN Z Y, SHA T, et al. Origin, genetic diversity, and population structure of Chinese domestic sheep [J]. Gene,2006,376(2):216-223.
[9] LIU J, DING X, ZENG Y, et al. Genetic diversity and phylogenetic evolution of Tibetan sheep based on mtDNA D-loop sequences [J]. PLoS One,2016,11(7):e0159308.
[10] 罗玉柱, 成述儒, BATSUURI L,等. 用mtDNA D-环序列探讨蒙古和中国绵羊的起源及遗传多样性[J]. 遗传学报, 2005, 32(12):1256-1265.
[11] ZHANG G, VAHIDI S M, MA Y H, et al. Limited polymorphisms of two Y-chromosomal SNPs in Chinese and Iranian sheep [J]. Anim Genet,2012,43(4):479-480.
[12] ZHANG M, PENG W F, YANG G L, et al. Y chromosome haplotype diversity of domestic sheep (Ovis aries) in northern Eurasia [J]. Anim Genet,2014,45(6):903-907.
[13] 赵君, 才让闹日, 郭淑珍,等. 欧拉羊与山谷型藏羊杂交F1代羔羊补饲兼放牧育肥试验效果观察[J]. 畜牧兽医杂志, 2011, 30(3):24-25.
[14] WANG X, CHEN H, LEI C Z. Genetic diversity and phylogenetic analysis of the mtDNA D-loop region in Tibetan sheep [J]. Asian-Australas J Anim Sci,2007,20(3):313-315.
[2] 郭万春, 郭占泽. 青海藏羊体尺、毛特性及相关性研究[J]. 黑龙江畜牧兽医, 2014(09上):77-79.
[3] ZHAO E, YU Q, ZHANG N, et al. Mitochondrial DNA diversity and the origin of Chinese indigenous sheep [J]. Trop Anim Health Prod,2013,45(8):1715-1722.
[4] 晁燕, 毛学荣, 雷良煜,等. 藏系绵羊mtDNA遗传结构及多样性研究[J]. 家畜生态学报, 2015, 36(10):16-21.
[5] GUANGXIN E, YONG-JU Z, RI-SU N, et al. Meta-analysis evidence of maternal lineages in Chinese Tibetan sheep using mtDNA D-loop panel [J]. Mitochondrial DNA A DNA Mapp Seq Anal, 2017,28(4):579-583.
[6] NIEMI M,BL?UER A,ISO-TOURU T, et al. Mitochondrial DNA and Y-chromosomal diversity in ancient populations of domestic sheep (ovis aries) in Finland: comparison with contemporary sheep breeds [J]. Genet Sel Evol,2013,45:2.
[7] ZENG X C, CHEN H Y, HUI W Q, et al. Genetic diversity measures of 8 local sheep breeds in northwest of China for genetic resource conservation [J]. Asian-Australas J Anim Sci,2010,23(12):1552-1556.
[8] CHEN S Y, DUAN Z Y, SHA T, et al. Origin, genetic diversity, and population structure of Chinese domestic sheep [J]. Gene,2006,376(2):216-223.
[9] LIU J, DING X, ZENG Y, et al. Genetic diversity and phylogenetic evolution of Tibetan sheep based on mtDNA D-loop sequences [J]. PLoS One,2016,11(7):e0159308.
[10] 罗玉柱, 成述儒, BATSUURI L,等. 用mtDNA D-环序列探讨蒙古和中国绵羊的起源及遗传多样性[J]. 遗传学报, 2005, 32(12):1256-1265.
[11] ZHANG G, VAHIDI S M, MA Y H, et al. Limited polymorphisms of two Y-chromosomal SNPs in Chinese and Iranian sheep [J]. Anim Genet,2012,43(4):479-480.
[12] ZHANG M, PENG W F, YANG G L, et al. Y chromosome haplotype diversity of domestic sheep (Ovis aries) in northern Eurasia [J]. Anim Genet,2014,45(6):903-907.
[13] 赵君, 才让闹日, 郭淑珍,等. 欧拉羊与山谷型藏羊杂交F1代羔羊补饲兼放牧育肥试验效果观察[J]. 畜牧兽医杂志, 2011, 30(3):24-25.
[14] WANG X, CHEN H, LEI C Z. Genetic diversity and phylogenetic analysis of the mtDNA D-loop region in Tibetan sheep [J]. Asian-Australas J Anim Sci,2007,20(3):313-315.