摘要
香花槐对城市不良环境有抗性,抗病力强,是造林的优良树种。分别对香花槐外植体灭菌时间、外植体初代培养、外植体继代培养进行初步研究,结果表明:利用0.1%升汞进行灭菌,在3.5~4.0 min最适宜;最佳初代培养的培养基为A6:MS+BA0.5+IBA0.1+蔗糖25 g+琼脂6 g;最佳继代培养的培养基为MS+BA0.3+I AA0.3+蔗糖30 g+琼脂6 g。
Rohinia idaho is an excellent tree species for afforestation because of its resistance to urban adverse environment and strong disease resistance. The sterilization time of rohinia idaho explants, the primary culture of explants and the secondary culture of explants were studied respectively. Results show that using 0.1% mercuric chloride to sterilization, in 3.5 ~ 4.0 min optimum; The optimal medium for primary culture was MS+BA0.5+IBA0.1+ sucrose 25 g+ AGAR 6 g; The optimal medium for secondary culture was B6:MS+BA0.3+I AA0.3+ sucrose 30 g+ AGAR 6 g.
引文
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