枝干番杏组织培养技术
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摘要
以枝干番杏的带叶茎段为外植体,比较不同灭菌方法的灭菌效果,及不同激素浓度对丛生芽诱导及生根的影响。结果表明:本研究中枝干番杏组织培养不经过愈伤组织阶段,芽诱导最适宜的培养基为MS+6-BA 8.325 mg·L~(-1)+NAA 16.65 mg·L~(-1)。芽丛生继代最适培养基为MS+6-BA 2.4 mg·L~(-1)+NAA 9.6 mg·L~(-1),根诱导最适培养基为1/2 MS+6-BA 1.2 mg·L~(-1)+NAA 4.8 mg·L~(-1)。枝干番杏的离体快繁体系有望用在番杏科其他植物的组培中。
The leaf stems of Dicrocaulon ramulosum(L.Bolus)Ihlenf were used as explants to compare the effects of different sterilization methods,as well as the effects of different hormone concentrations on the induction of shoots and roots.The results showed that the tissue culture did not pass through the callus stage.The optimal medium for shoot induction,bud subculture and root induction was MS+6-BA 8.325 mg·L~(-1)+NAA16.65 mg·L~(-1),MS+6-BA 2.4 mg·L~(-1)+NAA 9.6 mg·L~(-1),and 1/2 MS+6-BA 1.2 mg·L~(-1)+ NAA4.8 mg·L~(-1),respectively.The in vitro rapid propagation system of Dicrocaulon ramulosum(L.Bolus)Ihlenf was expected to be used in the tissue culture of other plants within the Aizoaceae family.
The leaf stems of Dicrocaulon ramulosum(L.Bolus)Ihlenf were used as explants to compare the effects of different sterilization methods,as well as the effects of different hormone concentrations on the induction of shoots and roots.The results showed that the tissue culture did not pass through the callus stage.The optimal medium for shoot induction,bud subculture and root induction was MS+6-BA 8.325 mg·L~(-1)+NAA16.65 mg·L~(-1),MS+6-BA 2.4 mg·L~(-1)+NAA 9.6 mg·L~(-1),and 1/2 MS+6-BA 1.2 mg·L~(-1)+ NAA4.8 mg·L~(-1),respectively.The in vitro rapid propagation system of Dicrocaulon ramulosum(L.Bolus)Ihlenf was expected to be used in the tissue culture of other plants within the Aizoaceae family.
引文
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[2] 范丽楠,张宗申,刘平武.生石花种子萌发及幼苗生长最优条件的筛选[J].安徽农业科学,2016(18): 123-126.
[3] 牟豪杰,王燕,吕永平,等.生石花植株离体再生及组培快繁研究[J].安徽农业科学,2016(33): 143-144,181.
[4] 陈香波,罗玉兰,田旗,等.露草的组织培养及植株再生[J].植物生理学通讯,2004(1): 63.
[5] 周静,杨苏文,方小波,等.照波的植物组织培养研究[J].贵州科学,2016,34(6): 13-17.
[6] 吴正景,黄雪娇,王柏,等.风铃玉的组织培养与快速繁殖[J].植物生理学报,2015,51(11): 2013-2016.
[7] 王小红,毕锦华,陆智伟,等.心叶日中花的组织培养与快速繁殖[J].植物生理学通讯,2009,45(8): 800.
[8] 刘红美,方小波,杨苏文,等.龙须海棠组织培养与试管内开花[J].植物生理学通讯,2009,45(7): 692.
[9] 赵海乐,孙国庆,冯凯,等.桃美人景天的组织培养与快速繁殖[J].吉林化工学院学报,2016,33(1): 54-60.
[10] 李春娟,单世华,万书波,等.花生胚小叶外植体再生影响因素研究简报[J].花生学报,2005,34(4): 36-38.