高乌甲素通过抑制小胶质细胞活化抑制炎症性疼痛
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摘要
目的探讨高乌甲素对炎症性疼痛的缓解作用及机制研究。方法采用完全弗氏佐剂(CFA)诱导大鼠验证疼痛模型,并测定其机械性刺激缩爪域值和热痛觉过敏阈值,评估高乌甲素预处理及后处理的疼痛缓解效果;利用免疫光、荧光定量PCR、Western blot法测定脊髓腰膨大L_(4-5)段GFAP及OX-42的表达情况,并进行统计学分析。结果①高乌甲素预处理组比后处理组有更好的疼痛缓解效果。②与正常组比较,溶剂组中GFAP及OX-42表达量显著升高(P<0.05)。③高乌甲素处理组能降低脊髓腰膨大L_(4-5)段OX-42的表达(P<0.05),然而对GFAP的表达没有显著差异(P>0.05)。结论高乌甲素能抑制小胶质细胞的活化,进而缓解炎症性疼痛。
Objective To investigate the alleviation effect and mechanism of lappaconitine on inflammatory pain.Methods The rat model of pain was induced by complete Freund's adjuvant(CFA),and the mechanical stimulation of the paw withdrawal field and thermal hyperalgesia threshold was determined to evaluate the pain relief effect of lappaconitine pretreatment and post treatment; Immunofluorescence,real-time PCR and Western blot were used to determine the expression of GFAP and OX-42 in L_(4-5) segment of spinal cord lumbar enlargement and statistical analysis.Results The lappaconitine pretreatment group had better pain relief effect than the post treatment group.Compared with the normal group,the expression levels of GFAP and OX-42 in the vehicle group were significantly increased(P<0.05).The lappaconitine treatment group could reduce the expression of OX-42 in L_(4-5) segment of spinal cord lumbar enlargement(P<0.05),but there was no significant difference in the expression of GFAP(P>0.05).Conclusion Lappaconitine inhibits the activation of microglia,which in turn relieves inflammatory pain.
Objective To investigate the alleviation effect and mechanism of lappaconitine on inflammatory pain.Methods The rat model of pain was induced by complete Freund's adjuvant(CFA),and the mechanical stimulation of the paw withdrawal field and thermal hyperalgesia threshold was determined to evaluate the pain relief effect of lappaconitine pretreatment and post treatment; Immunofluorescence,real-time PCR and Western blot were used to determine the expression of GFAP and OX-42 in L_(4-5) segment of spinal cord lumbar enlargement and statistical analysis.Results The lappaconitine pretreatment group had better pain relief effect than the post treatment group.Compared with the normal group,the expression levels of GFAP and OX-42 in the vehicle group were significantly increased(P<0.05).The lappaconitine treatment group could reduce the expression of OX-42 in L_(4-5) segment of spinal cord lumbar enlargement(P<0.05),but there was no significant difference in the expression of GFAP(P>0.05).Conclusion Lappaconitine inhibits the activation of microglia,which in turn relieves inflammatory pain.
引文
[1]高丽丽,康爽.高乌甲素在术后镇痛中的应用进展[J].黑龙江医学,2015,39(8):990-992.
[2]刘铭佩.高乌甲素对炎症性疼痛的镇痛作用[J].中国药物与临床,2003,3(3):244-245.
[3]Tanga FY,Nutilemcmenemy N,Deleo JA.The CNS role of Toll-like receptor 4 in innate neuroimmunity and painful neuropathy[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(16):5856-5861.
[4]Chaplan SR ,Bach FW ,Pogrel JW ,et al.Quantitative assessment of tactile allodynia in the rat paw[J].J Neurosci Methods,1994,53(1):55-63.
[5]Wieseler-Frank J,Maier SF,Watkins LR.Glial activation and pathological pain[J].Neurochemistry International,2004,45(2-3):389-395.
[6]Jha MK,Jeon S,Suk K.Glia as a Link between Neuroinflammation and Neuropathic Pain[J].Immune Network,2012,12(2):41-47.
[7]Skaper SD,Giusti P,Facci L.Microglia and mast cells:two tracks on the road to neuroinflammation.[J].Faseb Journal Official Publication of the Federation of American Societies for Experimental Biology,2012,26(8):3103.
[2]刘铭佩.高乌甲素对炎症性疼痛的镇痛作用[J].中国药物与临床,2003,3(3):244-245.
[3]Tanga FY,Nutilemcmenemy N,Deleo JA.The CNS role of Toll-like receptor 4 in innate neuroimmunity and painful neuropathy[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(16):5856-5861.
[4]Chaplan SR ,Bach FW ,Pogrel JW ,et al.Quantitative assessment of tactile allodynia in the rat paw[J].J Neurosci Methods,1994,53(1):55-63.
[5]Wieseler-Frank J,Maier SF,Watkins LR.Glial activation and pathological pain[J].Neurochemistry International,2004,45(2-3):389-395.
[6]Jha MK,Jeon S,Suk K.Glia as a Link between Neuroinflammation and Neuropathic Pain[J].Immune Network,2012,12(2):41-47.
[7]Skaper SD,Giusti P,Facci L.Microglia and mast cells:two tracks on the road to neuroinflammation.[J].Faseb Journal Official Publication of the Federation of American Societies for Experimental Biology,2012,26(8):3103.