PCR-DGGE技术在城镇供排水系统微生物多样性研究中的应用
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摘要
聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术已广泛应用于微生物分子生态学领域,成为研究微生物多样性常用的分子检测工具之一。在总结归纳PCR-DGGE技术的原理和特点的基础上,综述了PCR-DGGE技术在城镇供水系统和污水处理系统中微生物多样性分析等方面的研究进展,并展望了该技术今后的发展方向。
Polymerase Chain Reaction-denaturing Gradient Gel Electrophoresis(PCR-DGGE)technology has been widely applied in the field of microbial molecular ecology and has become one of the most popular molecular detection tools for microbial diversity research.On the basis of summarizing the principles and characteristics of PCR-DGGE technology,this paper reviews the research progress of PCR-DGGE in the analysis of microbial diversity in urban water supply and sewage treatment systems and looks forward to the future development direction of the technology.
Polymerase Chain Reaction-denaturing Gradient Gel Electrophoresis(PCR-DGGE)technology has been widely applied in the field of microbial molecular ecology and has become one of the most popular molecular detection tools for microbial diversity research.On the basis of summarizing the principles and characteristics of PCR-DGGE technology,this paper reviews the research progress of PCR-DGGE in the analysis of microbial diversity in urban water supply and sewage treatment systems and looks forward to the future development direction of the technology.
引文
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[25]Tian J,Lu J,Zhang Y,et al.Microbial community structures and dynamics in the O3/BAC drinking water treatment process[J].International Journal of Environmental Research&Public Health,2014,11(6):6281-6290.
[26]Ma G,Pei H,Hu W,et al.Study on microbial community structures in drinking water sludge by PCR-DGGE[J].Journal of Pure&Applied Microbiology,2013,7(1):467-477.
[27]Douterelo I,Sharpe R L,Boxall J B.Influence of hydraulic regimes on bacterial community structure and composition in an experimental drinking water distribution system[J].Water Research,2013,47(2):503-516.
[28]Roz·ej A,Cydzik-Kwiatkowska A,Kowalska B,et al.Structure and microbial diversity of biofilms on different pipe materials of a model drinking water distribution systems[J].World Journal of Microbiology&Biotechnology,2015,31(1):37-47.
[29]Roeder R S,Lenz J,Tarne P,et al.Long-term effects of disinfectants on the community composition of drinking water biofilms[J].International Journal of Hygiene and Environmental Health,2010,213(3):183-189.
[30]Aell H,Qi R,Yang M.Comparison of bacterial community structures in two systems of a sewage treatment plant using PCR-DGGE analysis[J].Journal of Environmental Sciences,2011,23(12):2049-2054.
[31]Boon N,De Windt W,Verstraete W,et al.Evaluation of nested PCR-DGGE(denaturing gradient gel electrophoresis)with group-specific 16SrRNA primers for the analysis of bacterial communities from different wastewater treatment plants[J].Fems Microbiology Ecology,2002,39(2):101-112.
[32]Liu X,Ren N,Yuan Y.Performance of a periodic anaerobic baffled reactor fed on Chinese traditional medicine industrial wastewater[J].Bioresource Technology,2009,100(1):104-110.
[33]牟洁,孙宝盛,陈谊.利用PCR-DGGE研究膜生物反应器中微生物的群落结构[J].环境科学学报,2010,30(4):729-734.
[34]Liu J,Zuo W,Zhang J,et al.Shifts in microbial community structure and diversity in a MBR combined with worm reactors treating synthetic wastewater[J].Journal of Environmental Sciences,2017,54(4):246-255.
[35]Xin W,Gong B,Jian Z,et al.Efficient simultaneous partial nitrification,anammox and denitrification(SNAD)system equipped with a real-time dissolved oxygen(DO)intelligent control system and microbial community shifts of different substrate concentrations[J].Water Research,2017,119:201-211.
[36]Li Y,Zhou J,Gong B,et al.Cometabolic degradation of lincomycin in a Sequencing Batch Biofilm Reactor(SBBR)and its microbial community[J].Bioresource Technology,2016,214:589-595.
[37]朱砺之,黄娟,傅大放,等.人工湿地生态系统中的微生物作用及PCR-DGGE技术的应用[J].安全与环境工程,2012,19(2):26-30.
[38]Adrados B,Sánchez O,Arias C A,et al.Microbial communities from different types of natural wastewater treatment systems:Vertical and horizontal flow constructed wetlands and biofilters[J].Water Research,2014,55(10):304-312.
[39]郭冀峰,徐成浩,王俞舒.PCR-DGGE技术在稳定塘底泥分析中的初步应用[J].安徽农业科学,2010,38(24):13318-13319.
[40]Hassanshahian M,Bayat Z,Cappello S,et al.Comparison the effects of bioaugmentation versus biostimulation on marine microbial community by PCR-DGGE:A mesocosm scale[J].Journal of Environmental Sciences,2016,43(5):136-146.
[41]Badia-Fabregat M,Lucas D,Tuomivirta T,et al.Study of the effect of the bacterial and fungal communities present in real wastewater effluents on the performance of fungal treatments[J].Science of the Total Environment,2017,579:366-377.
[2]Oliver J D.The viable but nonculturable state in bacteria[J].Journal of Microbiology,2005,43(1):93-100.
[3]Fischer S G,Lerman L S.Length-independent sepa-ration of DNA restriction fragments in two-dimensional gel electrophoresis[J].Cell,1979,16(1):191-200.
[4]Muyzer G,Waal E C D,Uitterlinden A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16SrRNA[J].Appl.Environ.Microbiol.,1993,59(3):695-700.
[5]Jousset A,Lara E,Nikolausz M,et al.Application of the denaturing gradient gel electrophoresis(DGGE)technique as an efficient diagnostic tool for ciliate communities in soil[J].Science of the Total Environment,2010,408(5):1221-1225.
[6]He Y,Chi J,Qi Y.Response of bacterial community structure to disappearance of phenanthrene and pyrene from sediment with different submerged macrophytes[J].Ecological Engineering,2016,91:207-211.
[7]Ruan A D,Liu C X.Analysis of effect of nicotine on microbial community structure in sediment using PCR-DGGE fingerprinting[J].Water Science and Engineering,2015,8(4):309-314.
[8]Diez B,Pedros-Alio C,Marsh T L,et al.Application of denaturing gradient gel electrophoresis(DGGE)to study the diversity of marine picoeukaryotic assemblages and comparison of DGGEwith other molecular techniques[J].Applied and Environmental Microbiology,2001,67(7):2942-2951.
[9]Richards T A,Vepritskiy A A,Gouliamova D E,et al.The molecular diversity of freshwater picoeukaryotes from an oligotrophic lake reveals diverse,distinctive and globally dispersed lineages[J].Environmental Microbiology,2005,7(9):1413-1425.
[10]Massana R,Guillou L,Diez B,et al.Unveiling the organisms behind novel eukaryotic ribosomal DNA sequences from the ocean[J].Applied and Environmental Microbiology,2002,68(9):4554-4558.
[11]蔡珉敏,田伟,王瑞霞,等.氧气与盐度对港西某油井本源微生物的影响[J].安全与环境工程,2014,21(3):7-11.
[12]Chang J J,Wu S Q,Liang K,et al.Comparative study of microbial community structure in integrated vertical-flow constructed wetlands for treatment of domestic and nitrified wastewaters[J].Environmental Science&Pollution Research International,2015,22(5):3518-3527.
[13]吴敏娜,武亚琦,屈艳,等.四种小鼠肠道微生物DNA提取方法比较[J].生态学杂志,2015,34(4):1183-1188.
[14]Korbie D J,Mattick J S.Touchdown PCR for increased specificity and sensitivity in PCR amplification[J].Nature Protocols,2008,3(9):1452-1456.
[15]邢德峰,任南琪,宋业颖,等.DG-DGGE分析产氢发酵系统微生物群落动态及种群多样性[J].生态学报,2005,25(7):1818-1823.
[16]James J B,Sherman T D,Devereux R.Analysis of bacterial communities in seagrass bed sediments by double-gradient denaturing gradient gel electrophoresis of PCR-amplified 16SrRNAgenes[J].Microb.Ecol.,2006,52(4):655-661.
[17]Sharaff M,Kamat S,Archana G.Analysis of copper tolerant rhizobacteria from the industrial belt of Gujarat,western India for plant growth promotion in metal polluted agriculture soils[J].Ecotoxicology&Environmental Safety,2017,138:113-121.
[18]Ding X,Wu C,Huang J,et al.Eubacterial and archaeal community characteristics in the man-made pit mud revealed by combined PCR-DGGE and FISH analyses[J].Food Research International,2014,62(8):1047-1053.
[19]He Q,Zhu Y,Fan L,et al.Effects of C/N ratio on nitrous oxide production from nitrification in a laboratory-scale biological aerated filter reactor[J].Water Science&Technology,2017,75(6):1270-1280.
[20]Ye W J,Tan J,Liu X L,et al.Temporal variability of cyanobacterial populations in the water and sediment samples of Lake Taihu as determined by DGGE and real-time PCR[J].Harmful Algae,2011,10(5):472-479.
[21]Wang X,Li Z,Su J,et al.Lysis of a red-tide causing alga,Alexandrium tamarense,caused by bacteria from its phycosphere[J].Biological Control,2010,52(2):123-130.
[22]Yang C Y,Li Y,Zhou Y,et al.Bacterial community dynamics during a bloom caused by Akashiwo sanguineain the Xiamen sea area,China[J].Harmful Algae,2012,20(14):132-141.
[23]Ishii N,Ishida S,Kagami M.PCR primers for assessing community structure of aquatic fungi including Chytridiomycota and Cryptomycota[J].Fungal Ecology,2015,13:33-43.
[24]张锡辉,王爽,王慧,等.水厂处理工艺中的微生物群落结构特征研究[J].中国给水排水,2007,23(13):36-40.
[25]Tian J,Lu J,Zhang Y,et al.Microbial community structures and dynamics in the O3/BAC drinking water treatment process[J].International Journal of Environmental Research&Public Health,2014,11(6):6281-6290.
[26]Ma G,Pei H,Hu W,et al.Study on microbial community structures in drinking water sludge by PCR-DGGE[J].Journal of Pure&Applied Microbiology,2013,7(1):467-477.
[27]Douterelo I,Sharpe R L,Boxall J B.Influence of hydraulic regimes on bacterial community structure and composition in an experimental drinking water distribution system[J].Water Research,2013,47(2):503-516.
[28]Roz·ej A,Cydzik-Kwiatkowska A,Kowalska B,et al.Structure and microbial diversity of biofilms on different pipe materials of a model drinking water distribution systems[J].World Journal of Microbiology&Biotechnology,2015,31(1):37-47.
[29]Roeder R S,Lenz J,Tarne P,et al.Long-term effects of disinfectants on the community composition of drinking water biofilms[J].International Journal of Hygiene and Environmental Health,2010,213(3):183-189.
[30]Aell H,Qi R,Yang M.Comparison of bacterial community structures in two systems of a sewage treatment plant using PCR-DGGE analysis[J].Journal of Environmental Sciences,2011,23(12):2049-2054.
[31]Boon N,De Windt W,Verstraete W,et al.Evaluation of nested PCR-DGGE(denaturing gradient gel electrophoresis)with group-specific 16SrRNA primers for the analysis of bacterial communities from different wastewater treatment plants[J].Fems Microbiology Ecology,2002,39(2):101-112.
[32]Liu X,Ren N,Yuan Y.Performance of a periodic anaerobic baffled reactor fed on Chinese traditional medicine industrial wastewater[J].Bioresource Technology,2009,100(1):104-110.
[33]牟洁,孙宝盛,陈谊.利用PCR-DGGE研究膜生物反应器中微生物的群落结构[J].环境科学学报,2010,30(4):729-734.
[34]Liu J,Zuo W,Zhang J,et al.Shifts in microbial community structure and diversity in a MBR combined with worm reactors treating synthetic wastewater[J].Journal of Environmental Sciences,2017,54(4):246-255.
[35]Xin W,Gong B,Jian Z,et al.Efficient simultaneous partial nitrification,anammox and denitrification(SNAD)system equipped with a real-time dissolved oxygen(DO)intelligent control system and microbial community shifts of different substrate concentrations[J].Water Research,2017,119:201-211.
[36]Li Y,Zhou J,Gong B,et al.Cometabolic degradation of lincomycin in a Sequencing Batch Biofilm Reactor(SBBR)and its microbial community[J].Bioresource Technology,2016,214:589-595.
[37]朱砺之,黄娟,傅大放,等.人工湿地生态系统中的微生物作用及PCR-DGGE技术的应用[J].安全与环境工程,2012,19(2):26-30.
[38]Adrados B,Sánchez O,Arias C A,et al.Microbial communities from different types of natural wastewater treatment systems:Vertical and horizontal flow constructed wetlands and biofilters[J].Water Research,2014,55(10):304-312.
[39]郭冀峰,徐成浩,王俞舒.PCR-DGGE技术在稳定塘底泥分析中的初步应用[J].安徽农业科学,2010,38(24):13318-13319.
[40]Hassanshahian M,Bayat Z,Cappello S,et al.Comparison the effects of bioaugmentation versus biostimulation on marine microbial community by PCR-DGGE:A mesocosm scale[J].Journal of Environmental Sciences,2016,43(5):136-146.
[41]Badia-Fabregat M,Lucas D,Tuomivirta T,et al.Study of the effect of the bacterial and fungal communities present in real wastewater effluents on the performance of fungal treatments[J].Science of the Total Environment,2017,579:366-377.