青海高原毛肉兼用半细毛羊血液蛋白多态性研究
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摘要
为了研究青海高原毛肉兼用半细毛羊血液蛋白多态性,试验采用聚丙烯酰胺凝胶垂直平板电泳法研究了29只青海高原毛肉兼用半细毛羊的HB、ES、AMY1、AMY2、AMY3及TF 6个蛋白基因位点的多态性,并计算和分析了青海高原毛肉兼用半细毛羊的遗传变异系数。结果表明:被检青海高原毛肉兼用半细毛羊血液蛋白的6个基因位点上,除AMY1和AMY3呈现单态外,其余均存在多态性;遗传变异系数中,平均基因均质度、平均基因杂合度、平均有效等位基因数分别为0.524 8,0.400 4,2.150 7。说明青海高原毛肉兼用细毛羊血液蛋白6个基因位点上,除AMY1和AMY3呈单态外,其余均存在多态性。
To study the blood protein polymorphism in the Qinghai Plateau semi- fine wool sheep for dual purpose wool / meat production,a method for vertical polyacrylamide slab gel electrophoresis was used to study the polymorphisms of the genetic loci of six proteins including hemoglobin( HB),esterase( ES),amylase( AMY1,AMY2,AMY3) and transferring( TF) from 29 Qinghai Plateau semi- fine wool sheep. And the coefficients of the genetic variation of the Qinghai Plateau semi- fine wool sheep were calculated and analyzed. The results showed that on the examined the genetic loci of the six blood proteins in the Qinghai Plateau semi- fine wool sheep,in addition to AMY1 and AMY3 showing monomorphism,the rest were polymorphic. Among the coefficients of genetic variation,the average gene homogeneity,average gene heterozygosity,and average effective number of alleles were 0. 524 8,0. 400 4,and 2. 150 7,respectively. The results indicate that on the examined the genetic loci of the six blood proteins in the Qinghai Plateau semi- fine wool sheep,in addition to AMY1 and AMY3 showing a single state,the rest are polymorphic.
To study the blood protein polymorphism in the Qinghai Plateau semi- fine wool sheep for dual purpose wool / meat production,a method for vertical polyacrylamide slab gel electrophoresis was used to study the polymorphisms of the genetic loci of six proteins including hemoglobin( HB),esterase( ES),amylase( AMY1,AMY2,AMY3) and transferring( TF) from 29 Qinghai Plateau semi- fine wool sheep. And the coefficients of the genetic variation of the Qinghai Plateau semi- fine wool sheep were calculated and analyzed. The results showed that on the examined the genetic loci of the six blood proteins in the Qinghai Plateau semi- fine wool sheep,in addition to AMY1 and AMY3 showing monomorphism,the rest were polymorphic. Among the coefficients of genetic variation,the average gene homogeneity,average gene heterozygosity,and average effective number of alleles were 0. 524 8,0. 400 4,and 2. 150 7,respectively. The results indicate that on the examined the genetic loci of the six blood proteins in the Qinghai Plateau semi- fine wool sheep,in addition to AMY1 and AMY3 showing a single state,the rest are polymorphic.
引文
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[15]张才骏.青海半细毛羊血液生化遗传多态性的研究[J].青海畜牧兽医杂志,2004,34(1):3-5.
[2]郭朝辉,才尕,贾玉刚.青海半细毛羊血红蛋白多态性的研究[J].草与畜杂志,1998(4):18-19.
[3]王应安,张寿,张勤文,等.青海毛肉兼用半细毛羊妊娠期外周血液中孕酮和雌二醇含量的测定[J].中国草食动物,1999(3):8-9.
[4]张才骏,张武学,张更利,等.青海省绵羊血红蛋白多态性的研究[J].青海畜牧兽医学院学报,1993,10(2):8-13.
[5]佐佐木清纲.家畜的血液型及其应用[M].李世安,译.上海:上海科学技术出版社,1982.
[6]铃木正三,池本卯典,向山明孝.比较血型学[M].程光潮,韩建林,杨华林,等译.北京:中国科学技术出版社,1991.
[7]胡能书,万国贤.同工酶技术及其应用[M].长沙:湖南科学技术出版社,1985.
[8]张才骏,张武学,张贞林,等.青海细毛羊血清淀粉酶的多态性研究[J].中国养羊,1994(4):22-24.
[9]张才骏.青海绵羊血清铁传递蛋白的电泳研究[J].动物医学进展,1997,18(3):23-26.
[10]张才骏,杨殿斌.青南藏羊血清酯酶的多态性[J].中国养羊,1992(4):31-33.
[11]张才骏,王勇,卢福山.欧拉羊血清酯酶多态性及其与血液指标的联系[J].青海畜牧兽医杂志,2009,39(1):1-3.
[12]张才骏,王勇,卢福山.欧拉羊血清淀粉酶同工酶酶谱的研究[J].青海畜牧兽医杂志,2007,37(1):3-5.
[13]刘霞,马海明,门正明.兰州大尾羊转铁蛋白多态性研究[J].甘肃农业大学学报,1999,34(4):358-360.
[14]杨俊年,崔泰保,鄢,等.小尾寒羊血浆转铁蛋白多态性与生长性状关系的研究[J].甘肃农业大学学报,2004,39(4):378-380.
[15]张才骏.青海半细毛羊血液生化遗传多态性的研究[J].青海畜牧兽医杂志,2004,34(1):3-5.