清达颗粒对自发性高血压大鼠心脏超声指标及病理学形态的影响
|
摘要
目的观察清达颗粒(QDG)对自发性高血压大鼠(SHR)血压、心脏超声指标及病理学检测的影响。方法将12只16周龄SHR大鼠按随机数字表法分为模型组(SHR组)、清达颗粒组(SHR+QDG组),6只同周龄Wistar大鼠为空白组(Wistar组)。SHR+QDG组给予清达颗粒0.8 g/(kg·d),每次灌药1 mL,连续给药8周,SHR组及Wistar组每天给予等量生理盐水。每4周检测1次血压,直到给药后第8周。在清达颗粒给药结束次日,使用彩色超声心动仪测量并记录各组大鼠左室室间隔厚度、左室内径、左室后壁厚度,并计算左室质量、射血分数(EF)和心肌缩短分数(FS)。HE染色法观察各组大鼠心脏病理学改变,Masson染色观察各组大鼠心肌间质及心肌壁内小动脉周围胶原纤维变化,并计算胶原蛋白容积分数(CVF)和心肌小动脉血管周围胶原面积与管腔面积比值(PVCA)。结果与SHR组比较,SHR+QDG组收缩压、舒张压、平均压均显著下降(P <0.05);SHR+QDG组左室后壁厚度、左室质量、CVF、PVCA较SHR组均显著下降(P <0.05),SHR+QDG组左室室间隔厚度、左室内径与SHR组比较差异无统计学意义(P>0.05)。结论清达颗粒可显著降低SHR血压,改善左心室功能并减轻心肌纤维化,对心肌细胞有保护作用。
Objective To investigate the effects of Qingda granules(QDG)on blood pressure,echocardiographic parameters and pathological detection in spontaneously hypertensive rats(SHR).Methods Twelve 16-week-old male SHR were randomly divided into model group(SHR group,n =6)and Qingda granule group(SHR+QDG group,n =6),respectively.Six Wistar rats were used as blank control group(Wistar group).The rats in SHR+QDG group were administered with0.8 g/(kg·d)at a dose of 1 mL each time for 8 weeks.The rats in SHR group and the Wistar group were given the same amount of normal saline per day.The blood pressure was measured every 4 weeks until the 8 th week after administration.The left ventricular septal thickness,left ventricular diameter,and left ventricular posterior wall thickness were measured and recorded by color echocardiography,and left ventricular mass,ejection fraction(EF),and myocardial fractional shortening(FS)were calculated on the day after the end of administration of QDG.The pathological changes of the hearts in rats were observed by Hematoxylin and Eosin(HE)staining.The changes of collagen fibers around the myocardial interstitial and small arterioles in myocardial wall of the rats were observed by Masson staining.The collagen volume fraction(CVF)and perivascular collagen area(PVCA)around the myocardial arterioles were calculated.Results Compared with the SHR group,the systolic,diastolic,and mean pressures were significantly decreased in the SHR+QDG group(P <0.05).The left ventricular posterior wall thickness,left ventricular mass,CVF,and PVCA in SHR+QDG group were significantly lower than those in SHR group(P <0.05).There was no significant difference in left ventricular septal thickness and left ventricular diameter between SHR+QDG group and SHR group(P >0.05).Conclusion QDG can significantly reduce blood pressure,improve left ventricular function,alleviate myocardial fibrosis,and protect cardiomyocytes in SHR.
Objective To investigate the effects of Qingda granules(QDG)on blood pressure,echocardiographic parameters and pathological detection in spontaneously hypertensive rats(SHR).Methods Twelve 16-week-old male SHR were randomly divided into model group(SHR group,n =6)and Qingda granule group(SHR+QDG group,n =6),respectively.Six Wistar rats were used as blank control group(Wistar group).The rats in SHR+QDG group were administered with0.8 g/(kg·d)at a dose of 1 mL each time for 8 weeks.The rats in SHR group and the Wistar group were given the same amount of normal saline per day.The blood pressure was measured every 4 weeks until the 8 th week after administration.The left ventricular septal thickness,left ventricular diameter,and left ventricular posterior wall thickness were measured and recorded by color echocardiography,and left ventricular mass,ejection fraction(EF),and myocardial fractional shortening(FS)were calculated on the day after the end of administration of QDG.The pathological changes of the hearts in rats were observed by Hematoxylin and Eosin(HE)staining.The changes of collagen fibers around the myocardial interstitial and small arterioles in myocardial wall of the rats were observed by Masson staining.The collagen volume fraction(CVF)and perivascular collagen area(PVCA)around the myocardial arterioles were calculated.Results Compared with the SHR group,the systolic,diastolic,and mean pressures were significantly decreased in the SHR+QDG group(P <0.05).The left ventricular posterior wall thickness,left ventricular mass,CVF,and PVCA in SHR+QDG group were significantly lower than those in SHR group(P <0.05).There was no significant difference in left ventricular septal thickness and left ventricular diameter between SHR+QDG group and SHR group(P >0.05).Conclusion QDG can significantly reduce blood pressure,improve left ventricular function,alleviate myocardial fibrosis,and protect cardiomyocytes in SHR.
引文
[1]陈伟伟,高润霖,刘力生,等.《中国心血管病报告2016》概要[J].中国循环杂志,2017,32(6):521-530.
[2]林凡礼.中国门诊高血压患者血压达标及靶器官损害筛查现状研究[D].上海:复旦大学,2013.
[3]郭士魁,陈可冀,张家鹏,等.关于高血压病中医分型的讨论[J].中医杂志,1960(3):4-5.
[4]曾雪慧.高血压中医证候特点及方药研究[D].北京:中国中医科学院,2007.
[5]UEDA H,MIYAWAKI M,HIRAOKA H.High-normal blood pressure is associated with new-onset electrocardiographic left ventricular hypertrophy[J].Journal of Human Hypertension,2015,29(1):9-13.
[6]SALAKO B L,OGAH O S,ADEBIYI A A,et al.Blood pressure control and left ventricular hypertrophy in hypertensive Nigerians[J].Annals of Cardiac Anaesthesia,2009,8(3):156-162.
[7]段练,熊兴江,王阶.高血压左室肥厚的病机及治疗[J].中华中医药杂志,2014(8):2566-2570.
[8]李颖,黄桢.中医药对H型高血压左室肥厚的临床研究进展[J].中西医结合心脑血管病杂志,2017,15(15):1843-1845.
[9]褚剑锋,吴广文,郑良扑,等.清眩降压汤对自发性高血压大鼠(SHR)大脑保护作用的实验研究[J].中医学报,2011,26(12):1462-1465.
[10]褚剑锋.清眩降压汤对SHR血压及心脏的影响[J].中西医结合心脑血管病杂志,2009,7(5):553-554.
[11]褚剑锋,吴广文,郑国华,等.清眩降压汤对自发性高血压模型大鼠肾脏的保护作用[J].中医杂志,2012,53(5):420-423.
[12]XIAO F,HE F,CHEN H,et al.Qingxuan Jiangya Decoction reverses vascular remodeling by inducing vascular smooth muscle cell apoptosis in spontaneously hypertensive rats[J].Molecules,2016,21(7):956.
[13]陈宏伟,包丽亚,黄月,等.清眩降压汤控制自发性高血压大鼠血压升高与抑制心肾大血管损害的药效学研究[J].康复学报,2017,27(2):28-32.
[2]林凡礼.中国门诊高血压患者血压达标及靶器官损害筛查现状研究[D].上海:复旦大学,2013.
[3]郭士魁,陈可冀,张家鹏,等.关于高血压病中医分型的讨论[J].中医杂志,1960(3):4-5.
[4]曾雪慧.高血压中医证候特点及方药研究[D].北京:中国中医科学院,2007.
[5]UEDA H,MIYAWAKI M,HIRAOKA H.High-normal blood pressure is associated with new-onset electrocardiographic left ventricular hypertrophy[J].Journal of Human Hypertension,2015,29(1):9-13.
[6]SALAKO B L,OGAH O S,ADEBIYI A A,et al.Blood pressure control and left ventricular hypertrophy in hypertensive Nigerians[J].Annals of Cardiac Anaesthesia,2009,8(3):156-162.
[7]段练,熊兴江,王阶.高血压左室肥厚的病机及治疗[J].中华中医药杂志,2014(8):2566-2570.
[8]李颖,黄桢.中医药对H型高血压左室肥厚的临床研究进展[J].中西医结合心脑血管病杂志,2017,15(15):1843-1845.
[9]褚剑锋,吴广文,郑良扑,等.清眩降压汤对自发性高血压大鼠(SHR)大脑保护作用的实验研究[J].中医学报,2011,26(12):1462-1465.
[10]褚剑锋.清眩降压汤对SHR血压及心脏的影响[J].中西医结合心脑血管病杂志,2009,7(5):553-554.
[11]褚剑锋,吴广文,郑国华,等.清眩降压汤对自发性高血压模型大鼠肾脏的保护作用[J].中医杂志,2012,53(5):420-423.
[12]XIAO F,HE F,CHEN H,et al.Qingxuan Jiangya Decoction reverses vascular remodeling by inducing vascular smooth muscle cell apoptosis in spontaneously hypertensive rats[J].Molecules,2016,21(7):956.
[13]陈宏伟,包丽亚,黄月,等.清眩降压汤控制自发性高血压大鼠血压升高与抑制心肾大血管损害的药效学研究[J].康复学报,2017,27(2):28-32.