基于质谱技术的神经肽研究进展
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摘要
神经肽是一类重要的内源活性物质,在神经系统中发挥重要的作用,并连接大脑和其他神经器官。基于质谱技术的神经肽组学研究旨在对神经肽进行大规模研究,在分子水平上得到重要信息,进一步加深对神经系统调控机制以及神经疾病致病机理的理解。文中综述了利用质谱技术进行神经肽研究的基本策略,包括样品处理、定性定量方法以及质谱成像等研究进展。
Neuropeptides are an important class of endogenous bioactive substances involved in the function of the nervous system, and connect the brain and other neural and peripheral organs. Mass spectrometry-based neuropeptidomics are designed to study neuropeptides in a large-scale manner and obtain important molecular information to further understand the mechanism of nervous system regulation and the pathogenesis of neurological diseases. This review summarizes the basic strategies for the study of neuropeptides using mass spectrometry, including sample preparation and processing, qualitative and quantitative methods, and mass spectrometry imagining.
Neuropeptides are an important class of endogenous bioactive substances involved in the function of the nervous system, and connect the brain and other neural and peripheral organs. Mass spectrometry-based neuropeptidomics are designed to study neuropeptides in a large-scale manner and obtain important molecular information to further understand the mechanism of nervous system regulation and the pathogenesis of neurological diseases. This review summarizes the basic strategies for the study of neuropeptides using mass spectrometry, including sample preparation and processing, qualitative and quantitative methods, and mass spectrometry imagining.
引文
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[27]Ross PL,Huang YN,Marchese JN,et al.Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents.Mol Cell Proteom,2004,3(12):1154-1169.
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[30]Hui LM,Xiang F,Zhang YZ,et al.Mass spectrometric elucidation of the neuropeptidome of a crustacean neuroendocrine organ.Peptides,2012,36(2):230-239.
[31]Ong SE,Blagoev B,Kratchmarova I,et al.Stable isotope labeling by amino acids in cell culture,SILAC,as a simple and accurate approach to expression proteomics.Mol Cell Proteom,2002,1(5):376-386.
[32]Boersema PJ,Aye TT,Van Veen TAB,et al.Triplex protein quantification based on stable isotope labeling by peptide dimethylation applied to cell and tissue lysates.Proteomics,2008,8(22):4624-4632.
[33]Kosanam H,Ramagiri S,Dass C.Quantification of endogenousα-andγ-endorphins in rat brain by liquid chromatography-tandem mass spectrometry.Anal Biochem,2009,392(1):83-89.
[34]Gessel MM,Norris JL,Caprioli RM.MALDIimaging mass spectrometry:spatial molecular analysis to enable a new age of discovery.JProteomics,2014,107:71-82.
[35]De Keyser SS,Kutz-Naber KK,Schmidt JJ,et al.Imaging mass spectrometry of neuropeptides in decapod crustacean neuronal tissues.J Proteome Res,2007,6(5):1782-1791.
[36]Ou Yang CZ,Chen BM,Li LJ.High throughput in situ DDA analysis of neuropeptides by coupling novel multiplex mass spectrometric imaging(MSI)with gas-phase fractionation.J Am Soc Mass Spectr,2015,26(12):1992-2001.
[37]Shariatgorji M,Nilsson A,Goodwin RJA,et al.Deuterated matrix-assisted laser desorption ionization matrix uncovers masked mass spectrometry imaging signals of small molecules.Aanl Chem,2012,84(16):7152-7157.
[38]Porta T,Grivet C,Kraemer T,et al.Single hair cocaine consumption monitoring by mass spectrometric imaging.Anal Chem,2011,83(11):4266-4272.
[2]Clynen E,Swijsen A,Raijmakers M,et al.Neuropeptides as targets for the development of anticonvulsant drugs.Mol Neurobiol,2014,50(2):626-646.
[3]Peters EMJ,Ericson ME,Hosoi J,et al.Neuropeptide control mechanisms in cutaneous biology:physiological and clinical significance.JInvest Dermatol,2006,126(9):1937-1947.
[4]H?kfelt T,Bartfai T,Bloom F.Neuropeptides:opportunities for drug discovery.Lancet Neurol,2003,2(8):463-472.
[5]Nelson MD,Trojanowski NF,George-Raizen JB,et al.The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans.Nat Commun,2013,4:2846.
[6]Che FY,Lim J,Pan H,et al.Quantitative neuropeptidomics of microwave-irradiated mouse brain and pituitary.Mol Cell Proteom,2005,4(9):1391-1405.
[7]Sturm RM,Dowell JA,Li L.Rat brain neuropeptidomics:tissue collection,protease inhibition,neuropeptide extraction,and mass spectrometric analysis.Methods Mol Biol,2010,615:217-226.
[8]Zhang XZ,Petruzziello F,Zani F,et al.High identification rates of endogenous neuropeptides from mouse brain.J Proteome Res,2012,11(5):2819-2827.
[9]Sturm RM,Greer T,Woodards N,et al.Mass spectrometric evaluation of neuropeptidomic profiles upon heat stabilization treatment of neuroendocrine tissues in crustaceans.J Proteome Res,2013,12(2):743-752.
[10]Secher A,Kelstrup CD,Conde-Frieboes KW,et al.Analytic framework for peptidomics applied to large-scale neuropeptide identification.Nat Commun,2016,7:11436.
[11]Chen RB,Hui LM,Cape SS,et al.Comparative neuropeptidomic analysis of food intake via a multifaceted mass spectrometric approach.Acs Chem Neurosci,2010,1(3):204-214.
[12]van Wanseele Y,de Prins A,de Bundel D,et al.Challenges for the in vivo quantification of brain neuropeptides using microdialysis sampling and LC-MS.Bioanalysis,2016,18(8):1965-1985.
[13]Fletcher HJ,Stenken JA.An in vitro comparison of microdialysis relative recovery of Met-and Leu-enkephalin using cyclodextrins and antibodies as affinity agents.Anal Chim Acta,2008,620(1/2):170-175.
[14]Schmerberg CM,Li LJ.Mass spectrometric detection of neuropeptides using affinity-enhanced microdialysis with antibody-coated magnetic nanoparticles.Anal Chem,2013,85(2):915-922.
[15]Behrens HL,Chen RB,Li LJ.Combining microdialysis,nano LC-MS,and MALDI-TOF/TOFto detect neuropeptides secreted in the crab,Cancer borealis.Anal Chem,2008,80(18):6949-6958.
[16]Jia CX,Lietz CB,Ye H,et al.A multi-scale strategy for discovery of novel endogenous neuropeptides in the crustacean nervous system.JProteomics,2013,91:1-12.
[17]Ye H,Wang JX,Zhang ZC,et al.Defining the neuropeptidome of the spiny Lobster panulirus interruptus brain using a multidimensional mass spectrometry-based platform.J Proteome Res,2015,14(11):4776-4791.
[18]Ma MM,Bors EK,Dickinson ES,et al.Characterization of the Carcinus maenas neuropeptidome by mass spectrometry and functional genomics.Gen Comp Endocr,2009,161(3):320-334.
[19]Hayakawa E,Menschaert G,de Bock PJ,et al.Improving the identification rate of endogenous peptides using electron transfer dissociation and collision-induced dissociation.J Proteome Res,2013,12(12):5410-5421.
[20]Southey BR,Amare A,Zimmerman TA,et al.Neuro Pred:a tool to predict cleavage sites in neuropeptide precursors and provide the masses of the resulting peptides.Nucleic Acids Res,2006,34:W267-W272.
[21]F?lth M,Sk?ld K,Norrman M,et al.Swe Pep,a database designed for endogenous peptides and mass spectrometry.Mol Cell Proteom,2006,5(6):998-1005.
[22]Ma B,Zhang KZ,Hendrie C,et al.PEAKS:powerful software for peptide de novo sequencing by tandem mass spectrometry.Rapid Commun Mass Spectrom,2003,17(20):2337-2342.
[23]Fu Q,Li LJ.De novo sequencing of neuropeptides using reductive isotopic methylation and investigation of ESI QTOF MS/MS fragmentation pattern of neuropeptides with N-terminal dimethylation.Anal Chem,2005,77(23):7783-7795.
[24]Frese CK,Boender AJ,Mohammed S,et al.Profiling of diet-induced neuropeptide changes in rat brain by quantitative mass spectrometry.Anal Chem,2013,85(9):4594-4604.
[25]Lee JE,Zamdborg L,Southey BR,et al.Quantitative peptidomics for discovery of circadian-related peptides from the rat suprachiasmatic nucleus.J Proteome Res,2013,12(2):585-593.
[26]Dayon L,Hainard A,Licker V,et al.Relative quantification of proteins in human cerebrospinal fluids by MS/MS using 6-plex isobaric tags.Anal Chem,2008,80(8):2921-2931.
[27]Ross PL,Huang YN,Marchese JN,et al.Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents.Mol Cell Proteom,2004,3(12):1154-1169.
[28]Frost DC,Greer T,Li LJ.High-resolution enabled12-plex Di Leu isobaric tags for quantitative proteomics.Anal Chem,2015,87(3):1646-1654.
[29]Chen RB,Xiao MM,Buchberger A,et al.Quantitative neuropeptidomics study of the effects of temperature change in the Crab Cancer borealis.J Proteome Res,2014,13(12):5767-5776.
[30]Hui LM,Xiang F,Zhang YZ,et al.Mass spectrometric elucidation of the neuropeptidome of a crustacean neuroendocrine organ.Peptides,2012,36(2):230-239.
[31]Ong SE,Blagoev B,Kratchmarova I,et al.Stable isotope labeling by amino acids in cell culture,SILAC,as a simple and accurate approach to expression proteomics.Mol Cell Proteom,2002,1(5):376-386.
[32]Boersema PJ,Aye TT,Van Veen TAB,et al.Triplex protein quantification based on stable isotope labeling by peptide dimethylation applied to cell and tissue lysates.Proteomics,2008,8(22):4624-4632.
[33]Kosanam H,Ramagiri S,Dass C.Quantification of endogenousα-andγ-endorphins in rat brain by liquid chromatography-tandem mass spectrometry.Anal Biochem,2009,392(1):83-89.
[34]Gessel MM,Norris JL,Caprioli RM.MALDIimaging mass spectrometry:spatial molecular analysis to enable a new age of discovery.JProteomics,2014,107:71-82.
[35]De Keyser SS,Kutz-Naber KK,Schmidt JJ,et al.Imaging mass spectrometry of neuropeptides in decapod crustacean neuronal tissues.J Proteome Res,2007,6(5):1782-1791.
[36]Ou Yang CZ,Chen BM,Li LJ.High throughput in situ DDA analysis of neuropeptides by coupling novel multiplex mass spectrometric imaging(MSI)with gas-phase fractionation.J Am Soc Mass Spectr,2015,26(12):1992-2001.
[37]Shariatgorji M,Nilsson A,Goodwin RJA,et al.Deuterated matrix-assisted laser desorption ionization matrix uncovers masked mass spectrometry imaging signals of small molecules.Aanl Chem,2012,84(16):7152-7157.
[38]Porta T,Grivet C,Kraemer T,et al.Single hair cocaine consumption monitoring by mass spectrometric imaging.Anal Chem,2011,83(11):4266-4272.