基于离子液体辅助氯霉素分子印迹聚合物的制备及应用
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摘要
以氯霉素(CAP)为模板,2-乙烯基吡啶(2-Vp)为功能单体,四氢呋喃和离子液体1-丁基-3-甲基咪唑四氟硼酸盐[BMIm]BF4的混合溶液为反应溶剂,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,合成了氯霉素的分子印迹及非印迹聚合物。优化功能单体、不同溶剂对印迹聚合物吸附性能的影响,结果表明,以2-乙烯基吡啶为功能单体,四氢呋喃和离子液体[BMIm]BF4(体积比1∶1)作为反应溶剂合成的分子印迹聚合物对氯霉素具有高的吸附容量,良好的特异性识别性能。氯霉素分子印迹聚合物的印迹因子为2.6,进行吸附-解吸附循环5次后,氯霉素印迹聚合物的性能稳定,可重复使用。将制备的氯霉素分子印迹聚合物作为富集材料,应用于鸡蛋样品中氯霉素的检测,回收率可达62.3%~81.1%,准确性好。
In this work, the molecularly imprinted polymers( MIPs) for chloramphenicol( CAP)( CAP- MIPs) were prepared by ionic liquids assistance using 2-vinylpyridine as functional monomer,ethylene glycol dimethacrylate( EGDMA) as the cross-linker and 2,2'-azobisisobutyronitrile( AIBN)as the initiator in the mixture of tetrahydrofuran and ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate( [BMIm]BF_4) as the porogenic solvent. The effects of functional monomer,progenic solvent on the binding capacity of MIPs were optimized. The MIPs possessed a high capacity and a favorable selectivity for CAP in the preparation of MIPs using 2-vinylpyridine as functional monomer and ionic liquid( [BMIm]BF_4)( 1 ∶ 1,by volume) as polymeric solvent. The imprinting factor of CAP- MIPs reached to 2. 6. The reusability of CAP- MIPs without any deterioration in capacity was demonstrated for five adsorption-desorption cycles. The developed method was applied in the enrichment and determination of CAP in spiked egg samples using CAP- MIPs as the adsorbent,with CAP recoveries of 62. 3%- 81. 1%.
In this work, the molecularly imprinted polymers( MIPs) for chloramphenicol( CAP)( CAP- MIPs) were prepared by ionic liquids assistance using 2-vinylpyridine as functional monomer,ethylene glycol dimethacrylate( EGDMA) as the cross-linker and 2,2'-azobisisobutyronitrile( AIBN)as the initiator in the mixture of tetrahydrofuran and ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate( [BMIm]BF_4) as the porogenic solvent. The effects of functional monomer,progenic solvent on the binding capacity of MIPs were optimized. The MIPs possessed a high capacity and a favorable selectivity for CAP in the preparation of MIPs using 2-vinylpyridine as functional monomer and ionic liquid( [BMIm]BF_4)( 1 ∶ 1,by volume) as polymeric solvent. The imprinting factor of CAP- MIPs reached to 2. 6. The reusability of CAP- MIPs without any deterioration in capacity was demonstrated for five adsorption-desorption cycles. The developed method was applied in the enrichment and determination of CAP in spiked egg samples using CAP- MIPs as the adsorbent,with CAP recoveries of 62. 3%- 81. 1%.
引文
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[3]Huang J F,Zhang H J,Feng Y Q.J.Agric.Food Chem.,2006,54:9279-9286.
[4]Huang J X,Hu Y F,Pan J L,Xu Z G,Li G K.Sci.China B(黄健祥,胡玉斐,潘加亮,许志刚,李攻科.中国科学B辑),2009,39:733-746.
[5]Wen Y Y,Chen L,Li J H,Liu D Y,Chen L X.Tr AC,Trends Anal.Chem.,2014,59:26-41.
[6]Kaufmann A,Butcher P,Maden K,Walker S,Widmer M.Anal.Chim.Acta,2015,862:41-52.
[7]Zhang Y,Li G K.J.Instrum.Anal.(张毅,李攻科.分析测试学报),2008,27(2):215-221.
[8]Hu Y F,Li G K.J.Instrum.Anal.(胡玉斐,李攻科.分析测试学报),2009,28(8):989-994.
[9]Chen L,Xu S,Li J.Chem.Soc.Rev.,2011,40:2922-2942.
[10]Mena M L,AgüíL,Martinez-Ruiz P,Yáňez-Sedeno P,Reviejo A J,Pingarrón J M.Anal.Bioanal.Chem.,2003,376:18-25.
[11]Shi X,Wu A,Zhang S,Li R,Zhang D.J.Chromatogr.B,2007,850:24-30.
[12]Shi X Z,Song S Q,Sun A L,Liu J H,Li D X,Chen J.Analyst,2012,137:3381-3389.
[13]Passerini S,Appeteccha G B.MRS Bull.,2013,38(7):540-547.
[14]Poole C F,Lenca N.Tr AC,Trends Anal.Chem.,2015,71:144-156.
[15]Wang H F,Zhu Y Z,Yan X P,Gao R Y,Zheng J Y.Adv.Mater.,2006,18:3266-3270.
[16]Sun X L,He J,Cai G R,Lin A Q,Zheng W J,Liu X,Chen L X,He X W,Zhang Y K.J.Sep.Sci.,2010,33:3786-3793.
[17]He C Y,Long Y Y,Pan J L,Li K,Liu F.Talanta,2008,74:1126-1131.