小麦矮秆种质山农11069-5矮秆基因的遗传分析及分子定位
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摘要
小麦矮秆种质山农11069-5株高65 cm左右,综合农艺性状较好。本研究对山农11069-5的赤霉酸反应和矮秆性状的遗传特点进行了鉴定分析,并对其矮秆基因进行了分子标记定位。结果表明,山农11069-5的矮秆性状受一个不完全显性主效基因控制,在苗期外施赤霉酸反应不敏感;利用1980对SSR、EST-SSR引物对山农11069-5的矮秆基因进行了连锁标记分析,筛选出3个连锁标记KSUM062、Wmc89和Wmc48,它们与矮秆基因的遗传距离分别为1.1 c M、3.4 c M和4.3 c M,其中KSUM062是一个EST-SSR标记;将山农11069-5的矮秆基因定位在染色体4BS上。
Shannong11069-5 is a dwarf wheat germplasm about 65 cm in length with good comprehensive agronomic traits. In this research, we carried out identifying GA response and genetic characteristics of dwarf traits in wheat dwarf germplasm of Shannong 11069-5, as well as mapping the dwarfing gene in Shannong 11069-5based on molecular markers. The results showed that the dwarf gene in Shannong11069-5 might be controlled by a partial dominant major gene; this dwarf gene was insensitive to GA treatment as well. 1980 pairs of SSR and EST-SSR primers were used to screen the dwarf gene in Shannong11069-5, and 3 linked markers Wmc48,Wmc89, and KSUM062 were obtained in this research. The genetic distance between these markers and the dwarf locus is about 1.1 c M、3.4 c M and 4.3 c M, respectively. The dwarf locus of Shannong11069-5 was mapped on4 BS in wheat chromosome.
Shannong11069-5 is a dwarf wheat germplasm about 65 cm in length with good comprehensive agronomic traits. In this research, we carried out identifying GA response and genetic characteristics of dwarf traits in wheat dwarf germplasm of Shannong 11069-5, as well as mapping the dwarfing gene in Shannong 11069-5based on molecular markers. The results showed that the dwarf gene in Shannong11069-5 might be controlled by a partial dominant major gene; this dwarf gene was insensitive to GA treatment as well. 1980 pairs of SSR and EST-SSR primers were used to screen the dwarf gene in Shannong11069-5, and 3 linked markers Wmc48,Wmc89, and KSUM062 were obtained in this research. The genetic distance between these markers and the dwarf locus is about 1.1 c M、3.4 c M and 4.3 c M, respectively. The dwarf locus of Shannong11069-5 was mapped on4 BS in wheat chromosome.
引文
Brner A.,Rder M.,and Korzun V.,1997,Comparative molecular mapping of GA insensitive Rht loci on chromosomes4B and 4D of common wheat(Triticum aestivum L.),Theor.Appl.Genet.,95(7):1133-1137
Chen G.L.,2011,Genetic analysis and molecular marker studyof a novel dwarfing gene in wheat Thinopyrum ponticumgermplasm line,Thesis for M.S.,Shandong AgriculturalUniversity,Supervisor:Li X.F.,pp.5(陈桂玲,2011,小麦-长穗偃麦草矮秆种质系的遗传分析与矮秆基因标记定位研究,硕士学位论文,山东农业大学,导师:李兴峰,pp.5)
Devos K.M.,Millan T.,and Gale M.D.,1993,Comparative RFLPmaps of the homoeologous group-2 chromosomes of wheat,rye and barley,Theor.Appl.Genet.,85(6-7):784-792
Ellis M.H.,Spielmeyer W.,Gale K.R.,Rebetzke G.J.,andRichards R.A.,2002,“Perfect”markers for the Rht-B1band Rht-D1b dwarfing genes in wheat,Theor.Appl.Genet.,105(6-7):1038-1042
Fu Y.,Wu J.H.,Wang C.Y.,Zhang B.J.,and Ji W.Q.,2011,Genetic and SSR analysis of dwarf gene in new wheatgermplasm N0381D,Mailei Zuowu Xuebao(Journal ofTriticeae Crops),31(3):411-415(付颖,吴金华,王长有,张保军,吉万全,2011,小麦新种质N0381D矮秆基因的遗传与SSR标记分析,麦类作物学报,31(3):411-415)
Jia J.Z.,Ding S.K.,Li Y.H.,Zhang H.,and Qi X.G.,1994,Chromosomal location of dwarfing genes in Wanyuan 50-2,a new dwarf line of wheat(Tr.aestivum),Zuowu Xuebao(Acta Agronomica Sinica),20(3):297-301(贾继增,丁寿康,李月华,张辉,齐秀改,1994,小麦新品系宛原50-2矮秆基因的染色体定位,作物学报,20(3):297-301)
Kang S.H.,2009,SSR targeting an d analysis of wheat dwarfinggene,Thesis for M.S.,Hebei Normal University,Supervisor:Bai F.,and Li X.P.,pp.21(康苏花,2009,小麦矮秆基因的SSR定位与分析,硕士学位论文,河北师范大学,导师:柏峰,李杏普,pp.21)
Lan S.Q.,and Li X.P.,2008,Progress on studies of molecular markerof Rht dwarfing genes in wheat,Hebei Nongye Kexue(Journal of Hebei Agricultural Sciences),12(1):69-71,74(兰素缺,李杏普,2008,小麦Rht矮秆基因的分子标记研究进展,河北农业科学,12(1):69-71,74)
Sun S.G.,Li Y.L.,Lu M.,Zhang J.,Wang X.J.,Wu J.,Zhao J.X.,Yang Q.H.,Liu S.H.,and Chen X.H.,2013,Distribution ofdwarfing genes in 67 American wheat cultivars detectedby molecular markers,Mailei Zuowu Xuebao(Journal ofTriticeae Crops),33(6):1087-1092(孙树贵,李艳丽,鲁敏,张军,王秀娟,武军,赵继新,杨群慧,刘淑会,陈新宏,2013,67份美国小麦品种矮秆基因的分子标记检测,麦类作物学报,33(6):1087-1092)
Sun X.P.,2001,Molecular markers linked to genes affectingplant height in wheat using a doubled-haploid population,Thesis for M.S.,Shanxi Agricultural University,Supervisor:Li R.Z.,pp.3-6(孙希平,2001,小麦株高基因的分子标记,硕士学位论文,山西农业大学,导师:李润植,pp.3-6)
Wan P.,Zhou Q.W.,Ma Z.Q.,Chen P.D.,and Liu D.J.,2001,Molecular markers linked to dwarf gene Rht3 in wheat,Yichuan Xuebao(Acta Genetica Sinica),28(11):1028-1033(万平,周青文,马正强,陈佩度,刘大钧,2001,小麦矮秆基因Rht3的RAPD和RFLP标记分析,遗传学报,28(11):1028-1033)
Wang G.,Hu T.Z.,Li X.J.,Dong N.,Feng S.W.,Li G.,Zhang L.L.,and Ru Z.G.,2012,Detection of dwarfing genes inwheat variety AK58 and its parents,He'nan Nongye Kexue(Journal of Henan Agricultural Sciences),41(9):22-25(王刚,胡铁柱,李小军,董娜,冯素伟,李淦,张立琳,茹振钢,2012,小麦新品种百农矮抗58及其亲本矮秆基因的检测,河南农业科学,41(9):22-25)
Wang Y.Y.,Zhang H.P.,Lai D.E.,Zhao Q.X.,Chang C.,and MaC.X.,2013,Detection of dwarf genes in 257 wheat varietyresources using molecular markers,Anhui Nongye DaxueXuebao(Journal of Anhui Agricultural University),40(5):860-866(王玉叶,张海萍,来得娥,赵秋霞,常成,马传喜,2013,257份小麦品种资源中矮秆基因的分子检测,安徽农业大学学报,40(5):860-866)
Worland A.J.,1986,Gibberellic acid insensitive dwarfing genesin southern european wheats,Euphytica,35(3):857-866
Xu J.Q.,He F.,Cui F.,Qi X.L.,Yu L.,Zhao C.H.,and Wang H.G.,2012,Molecular markers localization of powderymildew resistance gene in Tritileymus translocation lineShannong 6343,Fenzi Zhiwu Yuzhong(Molecular PlantBreeding),10(1):86-91(徐金秋,何方,崔法,亓晓蕾,余利,赵春华,王洪刚,2012,小滨麦易位系山农6343抗白粉病基因的分子标记定位,分子植物育种,10(1):86-91)
Xu X.B.,Zhang A.M.,Li X.H.,and Sun Y.T.,2001,Utilization ofdwarfing resources and development of dwarfing genesstudy in wheat,Henongxue Bao(Acta Agriculturae Nucleatae Sinica),15(3):188-192(徐相波,张爱民,李新华,孙永堂,2001,小麦矮源的利用和矮秆基因的研究进展,核农学报,15(3):188-192)
Yang Y.,2013,Identification of SSR markers linked to newwheat dwarf gene and analysis of cell wall in Hua Dwarf01,Thesis for M.S.,Huazhong Agricultural University,Supervisor:Sun D.F.,pp.19-23(杨悦,2013,小麦新矮源华矮01矮秆基因的SSR标记及其细胞壁成分的分析,硕士学位论文,华中农业大学,导师:孙东发,pp.19-23)
Zhao H.,2004,Advances in researches and applications of wheatRht genes,Heibei Nongye Kexue(Journal of Hebei Agricultural Sciences),8(4):96-99(赵和,2004,小麦矮秆基因研究和利用现状,河北农业科学,8(4):96-99)
Chen G.L.,2011,Genetic analysis and molecular marker studyof a novel dwarfing gene in wheat Thinopyrum ponticumgermplasm line,Thesis for M.S.,Shandong AgriculturalUniversity,Supervisor:Li X.F.,pp.5(陈桂玲,2011,小麦-长穗偃麦草矮秆种质系的遗传分析与矮秆基因标记定位研究,硕士学位论文,山东农业大学,导师:李兴峰,pp.5)
Devos K.M.,Millan T.,and Gale M.D.,1993,Comparative RFLPmaps of the homoeologous group-2 chromosomes of wheat,rye and barley,Theor.Appl.Genet.,85(6-7):784-792
Ellis M.H.,Spielmeyer W.,Gale K.R.,Rebetzke G.J.,andRichards R.A.,2002,“Perfect”markers for the Rht-B1band Rht-D1b dwarfing genes in wheat,Theor.Appl.Genet.,105(6-7):1038-1042
Fu Y.,Wu J.H.,Wang C.Y.,Zhang B.J.,and Ji W.Q.,2011,Genetic and SSR analysis of dwarf gene in new wheatgermplasm N0381D,Mailei Zuowu Xuebao(Journal ofTriticeae Crops),31(3):411-415(付颖,吴金华,王长有,张保军,吉万全,2011,小麦新种质N0381D矮秆基因的遗传与SSR标记分析,麦类作物学报,31(3):411-415)
Jia J.Z.,Ding S.K.,Li Y.H.,Zhang H.,and Qi X.G.,1994,Chromosomal location of dwarfing genes in Wanyuan 50-2,a new dwarf line of wheat(Tr.aestivum),Zuowu Xuebao(Acta Agronomica Sinica),20(3):297-301(贾继增,丁寿康,李月华,张辉,齐秀改,1994,小麦新品系宛原50-2矮秆基因的染色体定位,作物学报,20(3):297-301)
Kang S.H.,2009,SSR targeting an d analysis of wheat dwarfinggene,Thesis for M.S.,Hebei Normal University,Supervisor:Bai F.,and Li X.P.,pp.21(康苏花,2009,小麦矮秆基因的SSR定位与分析,硕士学位论文,河北师范大学,导师:柏峰,李杏普,pp.21)
Lan S.Q.,and Li X.P.,2008,Progress on studies of molecular markerof Rht dwarfing genes in wheat,Hebei Nongye Kexue(Journal of Hebei Agricultural Sciences),12(1):69-71,74(兰素缺,李杏普,2008,小麦Rht矮秆基因的分子标记研究进展,河北农业科学,12(1):69-71,74)
Sun S.G.,Li Y.L.,Lu M.,Zhang J.,Wang X.J.,Wu J.,Zhao J.X.,Yang Q.H.,Liu S.H.,and Chen X.H.,2013,Distribution ofdwarfing genes in 67 American wheat cultivars detectedby molecular markers,Mailei Zuowu Xuebao(Journal ofTriticeae Crops),33(6):1087-1092(孙树贵,李艳丽,鲁敏,张军,王秀娟,武军,赵继新,杨群慧,刘淑会,陈新宏,2013,67份美国小麦品种矮秆基因的分子标记检测,麦类作物学报,33(6):1087-1092)
Sun X.P.,2001,Molecular markers linked to genes affectingplant height in wheat using a doubled-haploid population,Thesis for M.S.,Shanxi Agricultural University,Supervisor:Li R.Z.,pp.3-6(孙希平,2001,小麦株高基因的分子标记,硕士学位论文,山西农业大学,导师:李润植,pp.3-6)
Wan P.,Zhou Q.W.,Ma Z.Q.,Chen P.D.,and Liu D.J.,2001,Molecular markers linked to dwarf gene Rht3 in wheat,Yichuan Xuebao(Acta Genetica Sinica),28(11):1028-1033(万平,周青文,马正强,陈佩度,刘大钧,2001,小麦矮秆基因Rht3的RAPD和RFLP标记分析,遗传学报,28(11):1028-1033)
Wang G.,Hu T.Z.,Li X.J.,Dong N.,Feng S.W.,Li G.,Zhang L.L.,and Ru Z.G.,2012,Detection of dwarfing genes inwheat variety AK58 and its parents,He'nan Nongye Kexue(Journal of Henan Agricultural Sciences),41(9):22-25(王刚,胡铁柱,李小军,董娜,冯素伟,李淦,张立琳,茹振钢,2012,小麦新品种百农矮抗58及其亲本矮秆基因的检测,河南农业科学,41(9):22-25)
Wang Y.Y.,Zhang H.P.,Lai D.E.,Zhao Q.X.,Chang C.,and MaC.X.,2013,Detection of dwarf genes in 257 wheat varietyresources using molecular markers,Anhui Nongye DaxueXuebao(Journal of Anhui Agricultural University),40(5):860-866(王玉叶,张海萍,来得娥,赵秋霞,常成,马传喜,2013,257份小麦品种资源中矮秆基因的分子检测,安徽农业大学学报,40(5):860-866)
Worland A.J.,1986,Gibberellic acid insensitive dwarfing genesin southern european wheats,Euphytica,35(3):857-866
Xu J.Q.,He F.,Cui F.,Qi X.L.,Yu L.,Zhao C.H.,and Wang H.G.,2012,Molecular markers localization of powderymildew resistance gene in Tritileymus translocation lineShannong 6343,Fenzi Zhiwu Yuzhong(Molecular PlantBreeding),10(1):86-91(徐金秋,何方,崔法,亓晓蕾,余利,赵春华,王洪刚,2012,小滨麦易位系山农6343抗白粉病基因的分子标记定位,分子植物育种,10(1):86-91)
Xu X.B.,Zhang A.M.,Li X.H.,and Sun Y.T.,2001,Utilization ofdwarfing resources and development of dwarfing genesstudy in wheat,Henongxue Bao(Acta Agriculturae Nucleatae Sinica),15(3):188-192(徐相波,张爱民,李新华,孙永堂,2001,小麦矮源的利用和矮秆基因的研究进展,核农学报,15(3):188-192)
Yang Y.,2013,Identification of SSR markers linked to newwheat dwarf gene and analysis of cell wall in Hua Dwarf01,Thesis for M.S.,Huazhong Agricultural University,Supervisor:Sun D.F.,pp.19-23(杨悦,2013,小麦新矮源华矮01矮秆基因的SSR标记及其细胞壁成分的分析,硕士学位论文,华中农业大学,导师:孙东发,pp.19-23)
Zhao H.,2004,Advances in researches and applications of wheatRht genes,Heibei Nongye Kexue(Journal of Hebei Agricultural Sciences),8(4):96-99(赵和,2004,小麦矮秆基因研究和利用现状,河北农业科学,8(4):96-99)