葫芦巴丸加减方对子宫内膜异位症大鼠相关神经生长因子的影响
|
摘要
目的:建立子宫内膜异位症大鼠模型,探讨葫芦巴丸加减方药理作用及对于子宫内膜异位症模型大鼠神经生长因子的影响。方法:选用雌性SD大鼠54只,随机分为假手术组、空白模型组、阳性药物对照组(孕三烯酮组)和中药低、中、高剂量组。采用自体移植法建立子宫内膜异位症大鼠模型。葫芦巴丸加减方治疗后取大鼠异位子宫内膜,免疫组化法检测异位内膜中神经生长因子(NGF)、络氨酸激酶(trkA)、P75神经营养素受体(P75NTR)的表达。结果:葫芦巴丸加减方可以有效降低异位内膜NGF、trkA、P75 NTR的表达水平(P<0.01)。并且在中药治疗组低、中、高剂量组中比较,中剂量组对NGF和P75 NTR的表达水平结果优于低、高剂量组(P<0.05)。结论:葫芦巴丸加减方治疗子宫内膜异位症的可能机制,是通过抑制NGF、trkA、P75 NTR等神经生长因子起作用。
Objective:By establishing a rat model of endometriosis(EMT)to explore the pharmacologic mechanism of Fenugreek bolus pill and observe the its effects on the expression of NGF,trkA and P75 NTR in rats.Methods:A total of 54 female SD rats were selected and randomly divided into the sham operation group,blank model group,positive drug control group(gestrienone group),low dose,medium dose and high dose groups of traditional Chinese medicine.A rat model of EMT was established by autologous transplantation.After treatment,the expression of NGF,trkA and P75 NTR in the eutopic and ectopic endometrium of rats were detected by immunohistochemistry.Results:Fenugreek bolus pill can effectively reduce the expression levels of ectopic endometrial NGF,trkA and P75 NTR(P<0.01).In addition,compared with the low-dose and high-dose groups,the results of the medium-dose group were better than the above two groups(P<0.05).Conclusion:The mechanism of Fenugreek bolus pill in treating EMT may alleviate pain by inhibiting NGF,trkA,P75 NTR and other nerve growth factors.
Objective:By establishing a rat model of endometriosis(EMT)to explore the pharmacologic mechanism of Fenugreek bolus pill and observe the its effects on the expression of NGF,trkA and P75 NTR in rats.Methods:A total of 54 female SD rats were selected and randomly divided into the sham operation group,blank model group,positive drug control group(gestrienone group),low dose,medium dose and high dose groups of traditional Chinese medicine.A rat model of EMT was established by autologous transplantation.After treatment,the expression of NGF,trkA and P75 NTR in the eutopic and ectopic endometrium of rats were detected by immunohistochemistry.Results:Fenugreek bolus pill can effectively reduce the expression levels of ectopic endometrial NGF,trkA and P75 NTR(P<0.01).In addition,compared with the low-dose and high-dose groups,the results of the medium-dose group were better than the above two groups(P<0.05).Conclusion:The mechanism of Fenugreek bolus pill in treating EMT may alleviate pain by inhibiting NGF,trkA,P75 NTR and other nerve growth factors.
引文
[1]张薇,沈洪沁,黄启玲,等.非动情期SD大鼠子宫内膜异位症模型的建立[J].中国实验动物学报,2004,12(1):21-24.
[2]黄继汉,黄晓晖,陈志扬,等.药理试验中动物间和动物与人体间的等效剂量换算[J].中国临床药理学与治疗学,2004,9(9):1069-1072.
[3]Zhang G,Dmitrie VAN,LIU Y,et al.Endome triosis as a neurovascular condition:estrous variations in innervation,vascularization,and growth factor content of ectopic endometrial cysts in the rat[J].Am J Physiol Regul Integr Comp Physiol,2008,294(1):R162-R171.
[4]Asantea,Tatlorrn.Endome triosis:the role of neuroangiogenesis[J].Annu Rev Physiol,2011,73:163-182.
[5]Hiriart M,Vidahamayo R,Sanchez-Soto MC.Nerve and fibroblast growth factors as modulators of pancreatic beta cell plasticity and insulin secretion[J].Isr Med Assoc,2001,3(2):114-116.
[6]Kajitani T,Maruyama T,Asada H,et al.Possible involvement of nerve growth factor in dysmenorrhea and dyspareunia associated with endometriosis[J].Endocr J,2013,60(10):1155-1164.
[7]Barcena de Arellano ML,Arnold J,Lang H,et al.Evidence of neurotrophic events due to peritoneal endometriotic lesions[J].Cytokine,2013,62(2):253-261.
[8]Arnold J,Barcenade Arellano ML,Ruster C,et al.Imbalance between sympathetic and sesory innervation in peritoneal endometriosis[J].Brain Behav Immun,2012,26(1):132-141.
[9]卢娇.神经生长因子与子宫内膜异位症痛经的相关性研究进展[J].医学综述,2011,17(6):831-833.
[10]李建勇,瞿全新,李宝江,等.神经生长因子受体在子宫腺肌病中的表达及意义[J].第三军医大学学报,2010,32(14):1570-1572.
[11]李晓燕,冷金花,郎景和.NGF及其受体trkA及p75NTR在子宫内膜异位症患者在位内膜中的表达及其与内异症疼痛的关系[J].现代妇产科进展,2012,20(6):435-439.
[12]Tokushige N,Markham R,Russell P,et al.Effects of hormonal treatment on nerve fibers in endometrium and myometrium in women with endometriosis[J].Fertil Steril,2008,90(5):1589-1598.
[13]Shi Z,Arai KY,Jin W,et al.Expression of nerve growth factor and its receptors NTRK1 and TNFRSF1B is regulated by estrogen and progesterone in the uteri of golden hamsters[J].Biol Reprod,2006,74(5):850-856.
[14]Chalar C,Richeri A,Viettro L,et al.Plasticity in developing rat uterine sensory nerves:the role of NGF and trkA[J].Cell Tissue Res,2003,314(2):191-205.
[2]黄继汉,黄晓晖,陈志扬,等.药理试验中动物间和动物与人体间的等效剂量换算[J].中国临床药理学与治疗学,2004,9(9):1069-1072.
[3]Zhang G,Dmitrie VAN,LIU Y,et al.Endome triosis as a neurovascular condition:estrous variations in innervation,vascularization,and growth factor content of ectopic endometrial cysts in the rat[J].Am J Physiol Regul Integr Comp Physiol,2008,294(1):R162-R171.
[4]Asantea,Tatlorrn.Endome triosis:the role of neuroangiogenesis[J].Annu Rev Physiol,2011,73:163-182.
[5]Hiriart M,Vidahamayo R,Sanchez-Soto MC.Nerve and fibroblast growth factors as modulators of pancreatic beta cell plasticity and insulin secretion[J].Isr Med Assoc,2001,3(2):114-116.
[6]Kajitani T,Maruyama T,Asada H,et al.Possible involvement of nerve growth factor in dysmenorrhea and dyspareunia associated with endometriosis[J].Endocr J,2013,60(10):1155-1164.
[7]Barcena de Arellano ML,Arnold J,Lang H,et al.Evidence of neurotrophic events due to peritoneal endometriotic lesions[J].Cytokine,2013,62(2):253-261.
[8]Arnold J,Barcenade Arellano ML,Ruster C,et al.Imbalance between sympathetic and sesory innervation in peritoneal endometriosis[J].Brain Behav Immun,2012,26(1):132-141.
[9]卢娇.神经生长因子与子宫内膜异位症痛经的相关性研究进展[J].医学综述,2011,17(6):831-833.
[10]李建勇,瞿全新,李宝江,等.神经生长因子受体在子宫腺肌病中的表达及意义[J].第三军医大学学报,2010,32(14):1570-1572.
[11]李晓燕,冷金花,郎景和.NGF及其受体trkA及p75NTR在子宫内膜异位症患者在位内膜中的表达及其与内异症疼痛的关系[J].现代妇产科进展,2012,20(6):435-439.
[12]Tokushige N,Markham R,Russell P,et al.Effects of hormonal treatment on nerve fibers in endometrium and myometrium in women with endometriosis[J].Fertil Steril,2008,90(5):1589-1598.
[13]Shi Z,Arai KY,Jin W,et al.Expression of nerve growth factor and its receptors NTRK1 and TNFRSF1B is regulated by estrogen and progesterone in the uteri of golden hamsters[J].Biol Reprod,2006,74(5):850-856.
[14]Chalar C,Richeri A,Viettro L,et al.Plasticity in developing rat uterine sensory nerves:the role of NGF and trkA[J].Cell Tissue Res,2003,314(2):191-205.