人参提取物对Ⅰ型干扰素及下游基因表达的调节作用
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摘要
目的探讨人参提取物对Ⅰ型干扰素及下游基因表达的调节作用。方法培养THP-1细胞并加入人参提取物后,ELISA检测干扰素-α、干扰素-β分泌水平,Real-time PCR检测BST-2、APOBEC3G表达。结果与空白组比较,人参提取物组干扰素-α分泌水平显著提高(P<0. 01),但对干扰素-β无明显影响(P>0. 05);与对照siRNA组比较,该组BST-2、APOBEC3G表达显著提高(P<0. 01),并与干扰素-α诱导呈正相关。结论人参提取物可通过Toll样受体途径诱导单核细胞分泌干扰素-α,从而提高抗病毒因子表达。
AIM To explore the regulatory effects of Ginseng Radix et Rhizoma extract on expressions of type Ⅰ interferons and downstream genes. METHODS After the addition of Ginseng Radix et Rhizoma extract into cultured THP-1 cells,the detection of interferon-α,interferon-β secretion levels and BST-2,APOBEC3 G expressions by ELISA and Real-time PCR were practiced,respectively. RESULTS Ginseng Radix et Rhizoma extract group exhibited significantly increased interferon-α secretion level( P<0. 01) without obvious effect on that of interferon-β( P>0. 05) when compared with the blank group; and it also demonstrated markedly increased BST-2,APOBEC3 G expressions( P<0. 01),showing a positive correlation with interferon-α induction when compared with siRNA control group. CONCLUSION Ginseng Radix et Rhizoma extract can induce interferon-α secretion of monocytes via Toll-like receptor pathway and thus improve the antiviral factors expressions.
AIM To explore the regulatory effects of Ginseng Radix et Rhizoma extract on expressions of type Ⅰ interferons and downstream genes. METHODS After the addition of Ginseng Radix et Rhizoma extract into cultured THP-1 cells,the detection of interferon-α,interferon-β secretion levels and BST-2,APOBEC3 G expressions by ELISA and Real-time PCR were practiced,respectively. RESULTS Ginseng Radix et Rhizoma extract group exhibited significantly increased interferon-α secretion level( P<0. 01) without obvious effect on that of interferon-β( P>0. 05) when compared with the blank group; and it also demonstrated markedly increased BST-2,APOBEC3 G expressions( P<0. 01),showing a positive correlation with interferon-α induction when compared with siRNA control group. CONCLUSION Ginseng Radix et Rhizoma extract can induce interferon-α secretion of monocytes via Toll-like receptor pathway and thus improve the antiviral factors expressions.
引文
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[13]黄蓉.核黄素磷酸钠联合干扰素治疗小儿疱疹性咽峡炎的疗效观察[J].现代药物与临床,2016,31(2):186-189.
[14]方燕.丙种球蛋白及干扰素治疗手足口病并发病毒性脑炎疗效观察[J].中国实用神经疾病杂志,2015,18(15):30-31.
[15]袁建丽,夏阳.扶正解毒祛湿汤联合重组人干扰素治疗宫颈HPV感染45例疗效观察[J].湖南中医杂志,2015,31(12):61-62.
[16]丁刘刚,郭晓蕾,马忠华,等.人参辅酶Q10复配制剂增强小鼠免疫力的实验研究[J].实用预防医学,2017,24(2):225-227.
[17]高文芹,贾力,赵余庆.人参的抗癌作用及其机制研究进展[J].药物评价研究,2011,34(1):53-58.
[18] Guo F,Mead J,Aliya N,et al. RO 90-7501 enhances TLR3and RLR agonist induced antiviral response[J]. PLoS One,2012,7(10):e42583.
[19] Trapp S,Derby N R,Singer R,et al. Double-stranded RNA analog poly(I∶C)inhibits human immunodeficiency virus amplification in dendritic cells via typeⅠinterferon-mediated activation of APOBEC3G[J]. J Virol,2009,83(2):884-895.
[20] Van D N,Goff D,Katsura C,et al. The interferon-induced protein BST-2/CD317 restricts release of virions from infected cells and is down-regulated from the cell surface by HIV-1 Vpu[J]. Cell Host Microbe,2008,3(4):245-252.
[2] Brennan K,Bowie A G. Activation of host pattern recognition receptors by viruses[J]. Curr Opin Microbiol,2010,13(4):503-507.
[3] Fernández-Suárez X M,Rigden D J,Galperin M Y. The 2014nucleic acids research database issue and an updated NAR online molecular biology database collection[J]. Nucleic Acids Res,2014,42(D1):D1-D6.
[4] Song J H,Choi H J,Song H H,et al. Antiviral activity of ginsenosides against coxsackievirus B3,enterovirus 71,and human rhinovirus 3[J]. J Ginseng Res,2014,38(3):173-179.
[5] Lee M H,Seo D J,Kang J H,et al. Expression of antiviral cytokines in Crandell-Reese feline kidney cells pretreated with Korean red ginseng extract or ginsenosides[J]. Food Chem Toxicol,2014,70:19-25.
[6] Lee M H,Lee B H,Lee S,et al. Reduction of hepatitis A virus on FRhK-4 cells treated with Korean red ginseng extract and ginsenosides[J]. J Food Sci,2013,78(9):M1412-M1415.
[7] Kang L J,Choi Y J,Lee S G. Stimulation of TRAF6/TAK1degradation and inhibition of JNK/AP-1 signalling by ginsenoside Rg3 attenuates hepatitis B virus replication[J]. Int J Biochem Cell Biol,2013,45(11):2612-2621.
[8] Yang H,Oh K H,Kim H J,et al. Ginsenoside-Rb2 and 20(S)-ginsenoside-Rg3 from Korean red ginseng prevent rotavirus infection in newborn mice[J]. J Microbiol Biotechnol,2018,28(3):391-396.
[9] Chan L Y,Kwok H H,Chan R W,et al. Dual functions of ginsenosides in protecting human endothelial cells against influenza H9N2-induced inflammation and apoptosis[J]. J Ethnopharmacol,2011,137(3):1542-1546.
[10] Lee M H,Lee B H,Jung J Y,et al. Antiviral effect of Korean red ginseng extract and ginsenosides on murine norovirus and feline calicivirus as surrogates for human norovirus[J]. J Ginseng Res,2011,35(4):429-435.
[11] Cho Y K,Kim J E. Effect of Korean Red Ginseng intake on the survival duration of human immunodeficiency virus type 1patients[J]. J Ginseng Res,2017,41(1):222-226.
[12]葛迎春.恩替卡韦联合长效干扰素治疗乙肝的临床研究[J].中国卫生标准管理,2015,6(9):202-203.
[13]黄蓉.核黄素磷酸钠联合干扰素治疗小儿疱疹性咽峡炎的疗效观察[J].现代药物与临床,2016,31(2):186-189.
[14]方燕.丙种球蛋白及干扰素治疗手足口病并发病毒性脑炎疗效观察[J].中国实用神经疾病杂志,2015,18(15):30-31.
[15]袁建丽,夏阳.扶正解毒祛湿汤联合重组人干扰素治疗宫颈HPV感染45例疗效观察[J].湖南中医杂志,2015,31(12):61-62.
[16]丁刘刚,郭晓蕾,马忠华,等.人参辅酶Q10复配制剂增强小鼠免疫力的实验研究[J].实用预防医学,2017,24(2):225-227.
[17]高文芹,贾力,赵余庆.人参的抗癌作用及其机制研究进展[J].药物评价研究,2011,34(1):53-58.
[18] Guo F,Mead J,Aliya N,et al. RO 90-7501 enhances TLR3and RLR agonist induced antiviral response[J]. PLoS One,2012,7(10):e42583.
[19] Trapp S,Derby N R,Singer R,et al. Double-stranded RNA analog poly(I∶C)inhibits human immunodeficiency virus amplification in dendritic cells via typeⅠinterferon-mediated activation of APOBEC3G[J]. J Virol,2009,83(2):884-895.
[20] Van D N,Goff D,Katsura C,et al. The interferon-induced protein BST-2/CD317 restricts release of virions from infected cells and is down-regulated from the cell surface by HIV-1 Vpu[J]. Cell Host Microbe,2008,3(4):245-252.