基于AMDIS和保留指数结合的GC-MS技术定性鉴别醋炙前后香附-艾叶药对中挥发油化学成分的变化
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摘要
目的从药对的整体角度切入,以调经止痛的功效为评价模型,来探究醋炙对香附-艾叶药对整体配伍的影响。方法采用GC-MS结合AMDIS与保留指数鉴别方法并联合主成分分析寻找共性规律的研究策略,全面系统地分析了醋炙前后香附-艾叶药对中挥发油化学成分的变化。结果香附-艾叶(生品)挥发油中共鉴别出54种化学成分,而香附-艾叶(醋品)挥发油中共鉴别出52种化学成分。醋炙前后的香附-艾叶药对与醋炙前后的单味药中挥发油化学成分的变化并不一致。从整体来看,在醋香附-醋艾叶药对的挥发油中,多数低沸点化合物的含量降低,而高沸点化合物无明显变化,且大部分低沸点化合物主要归属于艾叶,大部分高沸点化合物主要归属于香附,α-香附酮的相对含量也有所增加。在醋香附-醋艾叶药对的挥发油中含量明显增加的化合物多为去氧倍半萜类化合物、长链脂肪醇和长链脂肪酮,而萜类氧化衍生物的含量则有所降低。结论采用GC-MS结合AMDIS与保留指数鉴别方法并联合主成分分析寻找共性规律的研究策略,可全面、科学和快速地鉴别醋炙前后香附-艾叶药对中挥发油的差异。从整体上看,醋炙使得香附-艾叶药对中挥发油的毒性减小,安全性增加,稳固并小幅增强了调经止痛的功效。
OBJECTIVE The effects of vinegar-processing on overall compatibility of Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair were investigated based on assessment model of regulation on painful menstruation as efficacy from overall angle of the herb-pair.METHODS The experimental strategy to look for common regularity of GC-MS technology combined with principal component analysis and identification methods based on AMDIS and retention index was used to analyze the changes of chemical constituents from volatile oils between crude and vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair comprehensively and systematically.RESULTS Fifty-four chemical components had been identified in volatile oils of crude Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair while fifty-two chemical components had been identified in volatile oils of vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair.In addition,The changes of chemical constituents from volatile oils in crude and vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair were inconsistent with those in crude and vinegar-processed Cyperi Rhizoma(Artemisiae Argyi Folium).On the whole,in volatile oils of vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair,the content of most low boiling point compounds belonging to Artemisiae Argyi Folium was decreased while the content of most high boiling point compounds belonging to Cyperi Rhizoma had no obvious change,and the relative content ofα-cyperone was increased to some extent.The compounds with significantly increasing contents in volatile oils of vinegar-processed Cyperi RhizomaArtemisiae Argyi Folium herb-pair were deoxy-sesquiterpenoids,long chain fatty alcohols,and long chain aliphatic ketones.Nevertheless the content of terpenoid oxide derivatives was decreased.CONCLUSION By employing the strategy to look for common regularity of GC-MS technology combined with principal component analysis and identification methods based on AMDIS and retention index,we could identify the differences of chemical constituents from volatile oils between crude and vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair comprehensively,scientifically,and rapidly.Overall,the toxicity of volatile oils in Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair was decreased,the safety was increased,and the efficacy of regulation on painful menstruation was strengthened after vinegar-processing.The strategy used in this paper would be a certain reference significance for analyzing other complex systems on Chinese herbal medicines.
OBJECTIVE The effects of vinegar-processing on overall compatibility of Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair were investigated based on assessment model of regulation on painful menstruation as efficacy from overall angle of the herb-pair.METHODS The experimental strategy to look for common regularity of GC-MS technology combined with principal component analysis and identification methods based on AMDIS and retention index was used to analyze the changes of chemical constituents from volatile oils between crude and vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair comprehensively and systematically.RESULTS Fifty-four chemical components had been identified in volatile oils of crude Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair while fifty-two chemical components had been identified in volatile oils of vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair.In addition,The changes of chemical constituents from volatile oils in crude and vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair were inconsistent with those in crude and vinegar-processed Cyperi Rhizoma(Artemisiae Argyi Folium).On the whole,in volatile oils of vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair,the content of most low boiling point compounds belonging to Artemisiae Argyi Folium was decreased while the content of most high boiling point compounds belonging to Cyperi Rhizoma had no obvious change,and the relative content ofα-cyperone was increased to some extent.The compounds with significantly increasing contents in volatile oils of vinegar-processed Cyperi RhizomaArtemisiae Argyi Folium herb-pair were deoxy-sesquiterpenoids,long chain fatty alcohols,and long chain aliphatic ketones.Nevertheless the content of terpenoid oxide derivatives was decreased.CONCLUSION By employing the strategy to look for common regularity of GC-MS technology combined with principal component analysis and identification methods based on AMDIS and retention index,we could identify the differences of chemical constituents from volatile oils between crude and vinegar-processed Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair comprehensively,scientifically,and rapidly.Overall,the toxicity of volatile oils in Cyperi Rhizoma-Artemisiae Argyi Folium herb-pair was decreased,the safety was increased,and the efficacy of regulation on painful menstruation was strengthened after vinegar-processing.The strategy used in this paper would be a certain reference significance for analyzing other complex systems on Chinese herbal medicines.
引文
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[2]王坚,陈鸿平,刘友平,等.应用自动质谱退卷积定性系统拆分中药挥发油总离子流谱图中的重叠峰-以青皮为例[J].中国中药杂志,2013,38(10):1564-1569.WANG J,CHEN HP,LIU YP,et al.Deconvolution of overlapped peaks in total ion chromatogram of essential oil from Citri Reticulatae Pericarpium Viride by automated mass spectral deconvolution anddentification system[J].China J Chin Mater Med,2013,38(10):1564-1569.
[3]李宝丽,邓建玲,蔡欣,等.顶空固相微萃取-气质联用结合主成分分析研究纯葡萄汁的香气成分[J].中国食品学报,2016,16(4):258-270.LI BL,DENG JL,CAI X,et al.Studies on aroma components in pure grape juice by HS-SPME-GC-MS coupled with PCA[J].J Chin Instit Food Sci Tech,2016,16(4):258-270.
[4]迟雪洁,王会,黄伟,等.艾叶不同组分发挥镇痛作用的安全范围研究[J].中国药物警戒,2012,9(6):330-332.CHI XJ,WANG H,HUANG W,et al.The analgesic effect study on margin of safety on different components from folium artemisiae argyi[J].Chin J Pharmacovigil,2012,9(6):330-332.
[5]丁平,田友清,陈国胜,等.香附油滴丸抗炎镇痛作用及其物质基础研究[J].中国实验方剂学杂志,2013,19(20):172-176.DING P,TIAN YQ,CHEN GS,et al.Study on anti-inflammatory and analgesic activities of Xiangfuyou Diwan and its material basis[J].Chin J Exper Tradit Med Form,2013,19(20):172-176.