CXCR1基因编码区SNPs与荷斯坦牛泌乳性能和繁殖性状关联分析
|
摘要
研究旨在探索CXCR1(Chemokine(C-X-C motif)receptor 1)基因编码区SNPs与荷斯坦牛泌乳性状及部分繁殖性状间关系。用飞行时间质谱法检测865头荷斯坦牛CXCR1基因编码区c.642 A>G、c.816 C>A、c.980 A>G和c.1068 G>A 4个SNPs位点,同时收集采样牛2010年12月至2012年7月繁殖指标和2010年至2014年奶牛生产性能测定(Dairy herd improvement,DHI)记录。采用多因素方差分析法分析CXCR1基因CDS区SNPs基因型与测定日产奶量、乳脂率、乳蛋白率等泌乳性状和产犊间隔、产后首配泌乳天数、配种次数和妊娠泌乳天数关系。结果表明,CXCR1-642 A>G和CXCR1-980 A>G对乳中体细胞评分(Somatic cell score,SCS)影响显著(P<0.05),c.642 A>G、c.816 C>A、c.980 A>G和c.1068 G>A对乳中乳脂率、蛋白率和总固体影响显著(P<0.05)。CXCR1-980 A>G和CXCR1-1068 G>A位点AA型个体测定日产奶量显著高于AG和GG型(P<0.05),但CXCR1-980 A>G AA型乳蛋白率、SCS和总固体显著低于AG和GG型(P<0.05)。CXCR1-816 C>A位点对产犊间隔影响达显著水平(P=0.033),AC基因型个体产犊间隔显著低于AA型个体。而CXCR1-642 A>G、CXCR1-980 A>G和CXCR1-1068 G>A位点对产犊间隔、产后首配泌乳天数、配种次数以及妊娠泌乳天数均无显著性关联(P>0.05)。CXCR1-980 A>G和CXCR1-1068 G>A对荷斯坦牛产奶量和乳品质存在显著遗传效应,CXCR1 c.816 C>A位点对产犊间隔影响显著。CXCR1基因CDS区SNP可作为提高产奶量和乳品质、缩短产犊间隔候选分子标记之一。
The purpose of this study was to explore the association between the single nucleotide polymorphisms(SNPs) in the coding region of CXCR1(Chemokine(C-X-C motif) receptor 1) gene and milk production traits and partial reproductive traits of Holstein cattle. 4 SNPs in the CXCR1 gene coding region(c.642, A>G, c.816 C>A, c.980 A>G and c.1068 G>A) were detected by time-of-flight mass spectrometry. The recordings about calving intervals, days in milk at first breeding for every parity,breeding times and days in milk after pregnancy of 865 cattle was were collected in a large dairy farm from Dec. 2010 to July 2012. The DHI recordings were collected from 2010 to 2014. The association among the SNPs and milk production traits, calving intervals, days in milk after first breeding, breeding times and days in milk after pregnancy was analyzed by Multivariate analysis of variance. The results showed that CXCR1-642 A>G and CXCR1-980 A>G had significant effects on SCS in milk(P<0.05),c.642 A>G, c.816 C>A, c.980 A>G and c.1068 G>A had significant effects on fat percentage, milk protein percentage and total solids in milk(P<0.05). For CXCR1-980 A>G or CXCR1-1068 G>A, the daily milk yield of the individual with AA genotype was significantly higher than that of AG and GG(P<0.05), but milk protein percentage, total solids and SCS was significantly lower than that of AG and GG for CXCR1-980 A>G(P<0.05). CXCR1-816 C>A had significant effects on calving intervals(P=0.033),the calving intervals of individuals with AC genotype are significantly shorter than that with AA genotype.CXCR1-642 A>G, CXCR1-980 A>Ga and CXCR1-1068 G>A had no effect on calving intervals, days in milk after first breeding, breeding times and days in milk after pregnancy(P>0.05). CXCR1-980 A>G or CXCR1-1068 G>A had significant genetic effect. CXCR1 c.816 C>A had significant effect on calving interval. The SNPs in coding region of CXCR1 gene could provide reference for selective breeding about improving the milk yield and quality and shortening calving interval in Chinese Holstein.
The purpose of this study was to explore the association between the single nucleotide polymorphisms(SNPs) in the coding region of CXCR1(Chemokine(C-X-C motif) receptor 1) gene and milk production traits and partial reproductive traits of Holstein cattle. 4 SNPs in the CXCR1 gene coding region(c.642, A>G, c.816 C>A, c.980 A>G and c.1068 G>A) were detected by time-of-flight mass spectrometry. The recordings about calving intervals, days in milk at first breeding for every parity,breeding times and days in milk after pregnancy of 865 cattle was were collected in a large dairy farm from Dec. 2010 to July 2012. The DHI recordings were collected from 2010 to 2014. The association among the SNPs and milk production traits, calving intervals, days in milk after first breeding, breeding times and days in milk after pregnancy was analyzed by Multivariate analysis of variance. The results showed that CXCR1-642 A>G and CXCR1-980 A>G had significant effects on SCS in milk(P<0.05),c.642 A>G, c.816 C>A, c.980 A>G and c.1068 G>A had significant effects on fat percentage, milk protein percentage and total solids in milk(P<0.05). For CXCR1-980 A>G or CXCR1-1068 G>A, the daily milk yield of the individual with AA genotype was significantly higher than that of AG and GG(P<0.05), but milk protein percentage, total solids and SCS was significantly lower than that of AG and GG for CXCR1-980 A>G(P<0.05). CXCR1-816 C>A had significant effects on calving intervals(P=0.033),the calving intervals of individuals with AC genotype are significantly shorter than that with AA genotype.CXCR1-642 A>G, CXCR1-980 A>Ga and CXCR1-1068 G>A had no effect on calving intervals, days in milk after first breeding, breeding times and days in milk after pregnancy(P>0.05). CXCR1-980 A>G or CXCR1-1068 G>A had significant genetic effect. CXCR1 c.816 C>A had significant effect on calving interval. The SNPs in coding region of CXCR1 gene could provide reference for selective breeding about improving the milk yield and quality and shortening calving interval in Chinese Holstein.
引文
[1]马彦男,贺鹏迦,朱静,等.GHR基因F279Y位点突变对中国荷斯坦牛泌乳性状的影响[J].中国应用生理学杂志,2013,29(5):400-404.
[2]张润锋,陈宏,雷初朝,等.IGFBP-3基因PCR-RFLP多态性与中国荷斯坦牛泌乳性状的相关分析[J].中国畜牧杂志,2006,42(3):9-11.
[3]方远洋,宋雪梅,姜俊芳,等.IL8基因3'UTR SNPs与中国荷斯坦牛泌乳性状的关联分析[J].浙江农业学报,2015,27(3):338-342.
[4]Hoekstraa J,Vander Lugh A W,Vander W J,et al.Genetic and phenotypic parameters for milk production and fertility traits in upgraded dairy cattle[J].Livestock Production Science,1994,40(3):225-232.
[5]梁春年,阎萍,郭宪,等.牛繁殖性状候选基因的研究进展[J].中国畜牧兽医,201 l,38(8):103-106.
[6]于孟虎.荷斯坦牛体尺体重与繁殖性状的相关分析[J].中国奶牛,2002(4):30-31.
[7]郭翔羽,郭刚,李锡智,等.北京地区荷斯坦青年牛繁殖性状影响因素分析[J].中国奶牛,2011(24):1-6.
[8]廖想想,陈丹,张美荣,等.产犊季节和胎次对荷斯坦牛部分繁殖指标的影响[J].中国奶牛,2013(11):20-23.
[9]李艳华,王海浪,朱玉林,等.高产荷斯坦牛繁殖障碍的诱因分析及应对策略[J].中国奶牛,2014(15):18-22.
[10]汪聪勇,苏银池,陈江凌,等.荷斯坦牛的繁殖性状及影响因素分析[J].家畜生态学报,2015,36(10):45-48.
[11]周妍.荷斯坦奶牛繁殖性状相关的分子标记筛选与鉴定[D].武汉:华中农业大学,2013.
[12]李鹏,何举,赵国丽,等.荷斯坦牛Leptin基因外显子2多态性与繁殖力性状的相关性研究[J].中国奶牛,2011(6):4-7.
[13]赵佳强,周妍,于倩楠,等.中国荷斯坦奶牛Fox O1基因多态性及与繁殖性状关系研究[J].东北农业大学学报,2013,44(6):54-57.
[14]Paape M,Menrzad J,Zhao X,et al.Defense of the bovine mammary gland by polymorpho-nuclear neutrophil leukocytes[J].Journal of Mammary Gland Biology and Neoplasia,2002,7(2):109-121.
[15]Bannerman D D,Paae M J,Lee J W,et al.Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection[J].Clinical&Diagnostic Laboratory Immunology,2004,11(3):463-472.
[16]Leyva-baca I,Schenkel F,Sharma B S,et al.Identification of single nucleotide polymorphisms in the bovine CCL2,IL8,CCR2and IL8RA genes and their association with health and production in Canadian Holsteins[J].Animal Genetics,2007,38(3):198-202.
[17]Youngerman S M,Saxton A M,Pighetti G M.Novel single nucleotide polymorphism and haplotypes within the bovine CXCR2 gene[J].Immunogenetics,2004,56(5):355-359.
[18]Galv?o K N,Pighetti G M,Chenog S H,et al.Association between interleukin-8 receptor-α(CXCR1)polymorphism and disease incidence,production,reproduction,and survival in Holstein cows[J].Journal of Dairy Science,2011,94(4):2083-2091.
[19]周雷,王洪梅,王长法,等.中国荷斯坦牛CXCR1基因第二外显子新SNPs与乳腺炎的关联分析[J].畜牧兽医学报,2011,42(8):1063-1070.
[20]张美荣,廖想想,陈丹,等.澳系进口荷斯坦奶牛CXCR1基因遗传多态性与泌乳性状及体细胞评分的关联性分析[J].中国兽医学报,2014,34(1):159-164.
[21]王梦琦,倪炜,张慧敏,等.中国荷斯坦牛CXCR1基因编码区SNP突变对临床乳房炎和生产寿命的影响[J].中国农业科学,2017,50(12):2359-2370.
[22]Pighetti G M,Kojima C J,Wojakiewicz L,et al.The bovine CXCR1 gene is highly polymorphic[J].Veterinary Immunology and Immunopathology,2011,145(1/2):464-470.
[23]官久强,王洪梅,王长法,等.中国荷斯坦牛CXCR1基因编码区的遗传多态性[J].西北农林科技大学学报:自然科学版,2009,37(6):47-52.
[24]Leyva-Baca I,Schenkel F,Martin J,et al.Polymorphisms in the5'upstream regin of the CXCR1 chemokine receptor gene,and their association with somatic cell score in Holstein cattle in Canada[J].Journal of Dairy Science,2008,91(1):407-417.
[25]Goertz I,Baes C,Weimann C,et al.Association between single nucleotide polymorphisms in the CXCR1 gene and somatic cell score in Holstein dairy cattle[J].Journal of Dairy Science,2009,92(8):4018-22.
[26]叶东东,张孔杰,毛波涛,等.影响新疆地区荷斯坦牛产犊间隔的因素分析[J].新疆农业科学,2010,47(8):1643-1646.
[27]毛永江,陈仁金,陈莹,等.利用多因子降维法研究CXCR1和IL-8基因交互作用与中国荷斯坦牛乳房炎的关系[J].中国农业科学,2011,44(9):1908-1915.
[28]官永强.奶牛CXCR1及CXCR2基因多态性与乳腺炎相关性研究[D].成都:四川农业大学,2009.
[2]张润锋,陈宏,雷初朝,等.IGFBP-3基因PCR-RFLP多态性与中国荷斯坦牛泌乳性状的相关分析[J].中国畜牧杂志,2006,42(3):9-11.
[3]方远洋,宋雪梅,姜俊芳,等.IL8基因3'UTR SNPs与中国荷斯坦牛泌乳性状的关联分析[J].浙江农业学报,2015,27(3):338-342.
[4]Hoekstraa J,Vander Lugh A W,Vander W J,et al.Genetic and phenotypic parameters for milk production and fertility traits in upgraded dairy cattle[J].Livestock Production Science,1994,40(3):225-232.
[5]梁春年,阎萍,郭宪,等.牛繁殖性状候选基因的研究进展[J].中国畜牧兽医,201 l,38(8):103-106.
[6]于孟虎.荷斯坦牛体尺体重与繁殖性状的相关分析[J].中国奶牛,2002(4):30-31.
[7]郭翔羽,郭刚,李锡智,等.北京地区荷斯坦青年牛繁殖性状影响因素分析[J].中国奶牛,2011(24):1-6.
[8]廖想想,陈丹,张美荣,等.产犊季节和胎次对荷斯坦牛部分繁殖指标的影响[J].中国奶牛,2013(11):20-23.
[9]李艳华,王海浪,朱玉林,等.高产荷斯坦牛繁殖障碍的诱因分析及应对策略[J].中国奶牛,2014(15):18-22.
[10]汪聪勇,苏银池,陈江凌,等.荷斯坦牛的繁殖性状及影响因素分析[J].家畜生态学报,2015,36(10):45-48.
[11]周妍.荷斯坦奶牛繁殖性状相关的分子标记筛选与鉴定[D].武汉:华中农业大学,2013.
[12]李鹏,何举,赵国丽,等.荷斯坦牛Leptin基因外显子2多态性与繁殖力性状的相关性研究[J].中国奶牛,2011(6):4-7.
[13]赵佳强,周妍,于倩楠,等.中国荷斯坦奶牛Fox O1基因多态性及与繁殖性状关系研究[J].东北农业大学学报,2013,44(6):54-57.
[14]Paape M,Menrzad J,Zhao X,et al.Defense of the bovine mammary gland by polymorpho-nuclear neutrophil leukocytes[J].Journal of Mammary Gland Biology and Neoplasia,2002,7(2):109-121.
[15]Bannerman D D,Paae M J,Lee J W,et al.Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection[J].Clinical&Diagnostic Laboratory Immunology,2004,11(3):463-472.
[16]Leyva-baca I,Schenkel F,Sharma B S,et al.Identification of single nucleotide polymorphisms in the bovine CCL2,IL8,CCR2and IL8RA genes and their association with health and production in Canadian Holsteins[J].Animal Genetics,2007,38(3):198-202.
[17]Youngerman S M,Saxton A M,Pighetti G M.Novel single nucleotide polymorphism and haplotypes within the bovine CXCR2 gene[J].Immunogenetics,2004,56(5):355-359.
[18]Galv?o K N,Pighetti G M,Chenog S H,et al.Association between interleukin-8 receptor-α(CXCR1)polymorphism and disease incidence,production,reproduction,and survival in Holstein cows[J].Journal of Dairy Science,2011,94(4):2083-2091.
[19]周雷,王洪梅,王长法,等.中国荷斯坦牛CXCR1基因第二外显子新SNPs与乳腺炎的关联分析[J].畜牧兽医学报,2011,42(8):1063-1070.
[20]张美荣,廖想想,陈丹,等.澳系进口荷斯坦奶牛CXCR1基因遗传多态性与泌乳性状及体细胞评分的关联性分析[J].中国兽医学报,2014,34(1):159-164.
[21]王梦琦,倪炜,张慧敏,等.中国荷斯坦牛CXCR1基因编码区SNP突变对临床乳房炎和生产寿命的影响[J].中国农业科学,2017,50(12):2359-2370.
[22]Pighetti G M,Kojima C J,Wojakiewicz L,et al.The bovine CXCR1 gene is highly polymorphic[J].Veterinary Immunology and Immunopathology,2011,145(1/2):464-470.
[23]官久强,王洪梅,王长法,等.中国荷斯坦牛CXCR1基因编码区的遗传多态性[J].西北农林科技大学学报:自然科学版,2009,37(6):47-52.
[24]Leyva-Baca I,Schenkel F,Martin J,et al.Polymorphisms in the5'upstream regin of the CXCR1 chemokine receptor gene,and their association with somatic cell score in Holstein cattle in Canada[J].Journal of Dairy Science,2008,91(1):407-417.
[25]Goertz I,Baes C,Weimann C,et al.Association between single nucleotide polymorphisms in the CXCR1 gene and somatic cell score in Holstein dairy cattle[J].Journal of Dairy Science,2009,92(8):4018-22.
[26]叶东东,张孔杰,毛波涛,等.影响新疆地区荷斯坦牛产犊间隔的因素分析[J].新疆农业科学,2010,47(8):1643-1646.
[27]毛永江,陈仁金,陈莹,等.利用多因子降维法研究CXCR1和IL-8基因交互作用与中国荷斯坦牛乳房炎的关系[J].中国农业科学,2011,44(9):1908-1915.
[28]官永强.奶牛CXCR1及CXCR2基因多态性与乳腺炎相关性研究[D].成都:四川农业大学,2009.