刺五加种苗质量分级标准及其方法的优选
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摘要
目的:研究刺五加种苗质量,制定分级标准,并优选最佳分析方法。方法:观察并测量一年生不同产地(宝清、林口、七台河、东方红、依兰、阿城、饶河及亚布力)刺五加实生苗的株高,径粗、叶面积,根长,叶绿素含量等主要农艺性状。分别采用K-聚类分级法,主成分评价因子K-聚类分析法,标准差法进行分级,以不同级别种苗成活率为指标来评价3种分级方法。结果:直接K-聚类分析方法进行刺五加种苗的质量分级为最优方法,将刺五加种苗分为3级,其中I级种苗株高≥13 cm,茎粗≥0.37 cm,根长≥8 cm,叶面积≥28 cm2,叶绿素含量≤31(SPAD值,下同),主要所属产地为宝清,七台河地区;Ⅱ级种苗株高8～13 cm,茎粗0.30～0.37cm,根长6～8 cm,叶面积13～28 cm2,叶绿素含量31～32,主要所属产地为阿城,东方红,饶河地区;Ⅲ级种苗株高5～8 cm,茎粗0.26～0.30 cm,根长5～6 cm,叶面积5～13 cm2,叶绿素含量32～38,主要所属产地为亚布力、依兰、林口。结论:该实验初步建立了刺五加种苗的质量分级标准,并确定了宝清县和七台河为黑龙江省内育苗选种的优质产区,为刺五加种苗种植及人工栽培提供质量评价依据。
Objective:To study the quality of Acanthopanax senticosus seedlings,develop grading standards and optimize the best analytical method.Method:Observing and measuring the plant height,diameter,leaf area,root length,chlorophyll content and other main agronomic traits of A.senticosus seedlings from different habitats(Baoqing,Qitaihe,Dongfanghong,Yilan,Acheng,Raohe Linkou and Yabuli) in each year.Kcluster grading method,principal component evaluation factor K-cluster analysis method,standard deviation method for grading,three classification methods were evaluated with different levels of seedling survival rate as indicators.Result:The direct K-cluster analysis method was used to determine the quality of A.senticosus seedlings as the best method.The seedlings of A.senticosus were divided into 3 grades,among in the first level the seedling height is≥13 cm,the stem diameter is≥0.37 cm,the root length is≥8 cm,the leaf area is≥28 cm2,the chlorophyll content is≤31,the main origin is Baoqing,Qitaihe area.In the second level the seedling height is8-13 cm,the stem diameter is 0.30-0.37 cm,the root length is 6-8 cm,the leaf area is 13-28 cm2,the chlorophyll content is 31-32,and the main origin is Acheng,Dongfanghong,Raohe area.In the third level the seedling height is 5-8 cm,the stem diameter is 0.26-0.30 cm,the root length is 5-6 cm,the leaf area is 5-13 cm2,the chlorophyll content is 32-38,and the main origin is Yabuli,Yilan and Linkou.Conclusion:In this experiment,the quality grading standards of A.senticosus seedlings were preliminarily established,Baoqing and Qitaihe can be used as a high-quality production area for breeding seeds in the Heilongjiang province.which provided the basis for quality evaluation of A.senticosus seedling planting and artificial cultivation.
Objective:To study the quality of Acanthopanax senticosus seedlings,develop grading standards and optimize the best analytical method.Method:Observing and measuring the plant height,diameter,leaf area,root length,chlorophyll content and other main agronomic traits of A.senticosus seedlings from different habitats(Baoqing,Qitaihe,Dongfanghong,Yilan,Acheng,Raohe Linkou and Yabuli) in each year.Kcluster grading method,principal component evaluation factor K-cluster analysis method,standard deviation method for grading,three classification methods were evaluated with different levels of seedling survival rate as indicators.Result:The direct K-cluster analysis method was used to determine the quality of A.senticosus seedlings as the best method.The seedlings of A.senticosus were divided into 3 grades,among in the first level the seedling height is≥13 cm,the stem diameter is≥0.37 cm,the root length is≥8 cm,the leaf area is≥28 cm2,the chlorophyll content is≤31,the main origin is Baoqing,Qitaihe area.In the second level the seedling height is8-13 cm,the stem diameter is 0.30-0.37 cm,the root length is 6-8 cm,the leaf area is 13-28 cm2,the chlorophyll content is 31-32,and the main origin is Acheng,Dongfanghong,Raohe area.In the third level the seedling height is 5-8 cm,the stem diameter is 0.26-0.30 cm,the root length is 5-6 cm,the leaf area is 5-13 cm2,the chlorophyll content is 32-38,and the main origin is Yabuli,Yilan and Linkou.Conclusion:In this experiment,the quality grading standards of A.senticosus seedlings were preliminarily established,Baoqing and Qitaihe can be used as a high-quality production area for breeding seeds in the Heilongjiang province.which provided the basis for quality evaluation of A.senticosus seedling planting and artificial cultivation.
引文
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[11]马伟,张美琦,徐姣,等.短瓣金莲花种子及种苗质量分级标准[J].东北林业大学学报,2018,46(8):56-59.
[12]刘洪见,郑坚,张旭乐,等.主成分分析法在杂交石竹种苗质量评价上的应用[J].农业科技通讯,2017(12):157-160.
[13]Inoue Y,Guérif,M,Baret F,et al.Simple and robust methods for remote sensing of canopy chlorophyll content:a comparative analysis of hyperspectral data for different types of vegetation[J].Plant Cell Environ,2016,39(12):2609-2623.
[14]Thomidis T,Zioziou E,Koundouras S,et al.Effect of prohexadione-Ca on leaf chlorophyll content,gas exchange,berry size and composition,wine quality and disease susceptibility in Vitisvinifera L.cv Xinomavro[J].Sci Hortic,2018,238:369-374.
[2]汪松,解焱.中国物种红色名录.第一卷[M].北京高等教育出版社,2004:101.
[3]高娜,孙永军,张建军,等.中药材种子种苗质量分级标准研究进展[J].中国中医药信息杂志,2018,25(4):129-132.
[4]禹娟红,张尚智.黄芪种子种苗质量标准研究进展[J].中兽医医药杂志,2018,37(4):95-96.
[5]沈宏伟,张爽,李佳宾,等.不同浓度的外源NO对刺五加种子激素及酶含量变化的影响[J].中国实验方剂学杂志,2019,doi:10.13422/j.cnki.syfjx.20190110.
[6]丁晓纲,刘喻娟,张晓珊,等.不同浓度指数施肥对美丽异木棉等3个树种幼苗生长的影响[J].生态环境学报,2013,22(4):619-624.
[7]李俊仁,陈秀珍,梁凌玲,等.岗梅种苗质量分级标准研究[J].种子,2016,35(3):115-118.
[8]孙长生,朱虹,龙祥友.山豆根种苗质量的分级指标探讨[J].贵州农业科学,2016,44(1):140-142.
[9]张玉,戚莹雪,王蕾,等.丹参种子种苗质量标准研究进展[J].中国种业,2018(6):8-12.
[10]李颖,黄璐琦,张小波,等.中药材种子种苗繁育基地建设进展概况[J].中国中药杂志,2017,42(22):4262-4265.
[11]马伟,张美琦,徐姣,等.短瓣金莲花种子及种苗质量分级标准[J].东北林业大学学报,2018,46(8):56-59.
[12]刘洪见,郑坚,张旭乐,等.主成分分析法在杂交石竹种苗质量评价上的应用[J].农业科技通讯,2017(12):157-160.
[13]Inoue Y,Guérif,M,Baret F,et al.Simple and robust methods for remote sensing of canopy chlorophyll content:a comparative analysis of hyperspectral data for different types of vegetation[J].Plant Cell Environ,2016,39(12):2609-2623.
[14]Thomidis T,Zioziou E,Koundouras S,et al.Effect of prohexadione-Ca on leaf chlorophyll content,gas exchange,berry size and composition,wine quality and disease susceptibility in Vitisvinifera L.cv Xinomavro[J].Sci Hortic,2018,238:369-374.