高效液相色谱法测定逍遥分散片中甘草苷含量
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摘要
目的建立测定逍遥分散片中甘草苷含量的高效液相色谱法。方法色谱柱采用Agilent SB-C_(18)柱(250 mm×4. 6 mm,5μm),流动相为乙腈-0. 05%醋酸溶液(10∶90),流速为1. 0 mL/min,检测波长为276 nm。结果甘草苷进样量在0. 020 78～0. 207 80μg范围内与峰面积线性关系良好,r=0. 999 9(n=7),平均回收率为97. 41%,RSD为0. 62%(n=6)。结论该方法简便、准确,重复性良好,可用作逍遥分散片的质量控制。
Objective To establish an HPLC method for content determination of liquoritin in Xiaoyao Dispersible Tablets. Methods The Agilent SB-C_(18) column(250 mm × 4. 6 mm,5 μm) was adopted with the mobile phase of acetonitrile-0. 05% acetic acid solution(10 ∶ 90),the flow rate was 1. 0 mL/min, the detection wavelength was 276 nm. Results Liquoritin showed a linear relationship within the range of 0. 020 78-0. 207 80 μg, r = 0. 999 9( n = 7), the average recovery rate was 97. 41%, and RSD was 0. 62%( n = 6).Conclusion The method is simple,accurate and reproducible,which can be used for the quality control of Xiaoyao Dispersible Tablets.
Objective To establish an HPLC method for content determination of liquoritin in Xiaoyao Dispersible Tablets. Methods The Agilent SB-C_(18) column(250 mm × 4. 6 mm,5 μm) was adopted with the mobile phase of acetonitrile-0. 05% acetic acid solution(10 ∶ 90),the flow rate was 1. 0 mL/min, the detection wavelength was 276 nm. Results Liquoritin showed a linear relationship within the range of 0. 020 78-0. 207 80 μg, r = 0. 999 9( n = 7), the average recovery rate was 97. 41%, and RSD was 0. 62%( n = 6).Conclusion The method is simple,accurate and reproducible,which can be used for the quality control of Xiaoyao Dispersible Tablets.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:1357,86-87.
[2]国家药典委员会.中华人民共和国药典(四部)[M].北京:中国医药科技出版社,2015:通则0100.
[3]代玉玲,普俊学,廖荣,等.中药分散片与其他中药口服制剂特点比较[J].中国民族民间医药,2017,26(8):63-65.
[4]张玉龙,王梦月,杨静玉.炙甘草化学成分及药理作用研究进展[J].上海中医药大学学报,2015,29(3):99-102.
[5]刘海燕.《伤寒论》蜜炙甘草临证作用探讨[J].北京中医药,2016,35(9):868-870.
[6]刘,荆鲁.炙甘草汤治疗心律失常研究进展[J].中国中药杂志,2007,32(23):2471-2473.
[7]海青山,郑梅.炙甘草汤临床应用与研究进展[J].中医药导报,2006,12(10):77-78.
[8]高晓南.大剂量白芍、炙甘草配伍对解痉的作用分析[J].亚太传统医药,2016,12(22):17-18.
[9]邰勋,郭英华.炙甘草汤加西药对冠心病心律失常的治疗作用研究[J].中国实用医药,2018,13(12):105-106.
[10]刘巍,鲁卫星.炙甘草汤抗快速性心律失常研究概况[J].中医杂志,2012,53(4):348-351.
[11]韩慧琴,王晓青,尹贻珍.高效液相法测定逍遥丸(水丸)中甘草苷的含量[J].中国药业,2012,21(13):21-22.
[12]郭明晔,张燕玲,李洋,等. HPLC法同时测定甘草及甘草头中4种成分含量[J].世界科学技术-中医药现代化,2014,16(2):358-362.
[13]成英,宋九华. HPLC测定逍遥丸中甘草苷、异甘草素和甘草酸的含量[J].中国现代应用药学,2012,29(2):163-166.
[14]杜蓉,张孟佑. HPLC法测定加味逍遥丸中芍药苷与甘草苷的含量[J].中国药房,2015,26(18):2571-2572.
[2]国家药典委员会.中华人民共和国药典(四部)[M].北京:中国医药科技出版社,2015:通则0100.
[3]代玉玲,普俊学,廖荣,等.中药分散片与其他中药口服制剂特点比较[J].中国民族民间医药,2017,26(8):63-65.
[4]张玉龙,王梦月,杨静玉.炙甘草化学成分及药理作用研究进展[J].上海中医药大学学报,2015,29(3):99-102.
[5]刘海燕.《伤寒论》蜜炙甘草临证作用探讨[J].北京中医药,2016,35(9):868-870.
[6]刘,荆鲁.炙甘草汤治疗心律失常研究进展[J].中国中药杂志,2007,32(23):2471-2473.
[7]海青山,郑梅.炙甘草汤临床应用与研究进展[J].中医药导报,2006,12(10):77-78.
[8]高晓南.大剂量白芍、炙甘草配伍对解痉的作用分析[J].亚太传统医药,2016,12(22):17-18.
[9]邰勋,郭英华.炙甘草汤加西药对冠心病心律失常的治疗作用研究[J].中国实用医药,2018,13(12):105-106.
[10]刘巍,鲁卫星.炙甘草汤抗快速性心律失常研究概况[J].中医杂志,2012,53(4):348-351.
[11]韩慧琴,王晓青,尹贻珍.高效液相法测定逍遥丸(水丸)中甘草苷的含量[J].中国药业,2012,21(13):21-22.
[12]郭明晔,张燕玲,李洋,等. HPLC法同时测定甘草及甘草头中4种成分含量[J].世界科学技术-中医药现代化,2014,16(2):358-362.
[13]成英,宋九华. HPLC测定逍遥丸中甘草苷、异甘草素和甘草酸的含量[J].中国现代应用药学,2012,29(2):163-166.
[14]杜蓉,张孟佑. HPLC法测定加味逍遥丸中芍药苷与甘草苷的含量[J].中国药房,2015,26(18):2571-2572.